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Protein

Ras-related protein Rab-13

Gene

RAB13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.6 Publications

Activity regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab may be activated by DENND1C, a guanine exchange factor. Activated in response to insulin.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1445148 Translocation of SLC2A4 (GLUT4) to the plasma membrane
R-HSA-8873719 RAB geranylgeranylation
R-HSA-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-13
Alternative name(s):
Cell growth-inhibiting gene 4 protein
Gene namesi
Name:RAB13
ORF Names:GIG4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143545.8
HGNCiHGNC:9762 RAB13
MIMi602672 gene
neXtProtiNX_P51153

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22T → N: Dominant negative. 1 Publication1
Mutagenesisi67Q → L: Constitutively active mutant locked in the active GTP-bound form. Impairs transports of cargo from the trans-Golgi network to the recycling endosomes and alters the assembly of functional tight junctions. 1 Publication1

Organism-specific databases

DisGeNETi5872
OpenTargetsiENSG00000143545
PharmGKBiPA34103

Polymorphism and mutation databases

DMDMi1710016

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211821 – 200Ras-related protein Rab-13Add BLAST200
PropeptideiPRO_0000370756201 – 203Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei178PhosphoserineCombined sources1
Modified residuei200Cysteine methyl esterSequence analysis1
Lipidationi200S-geranylgeranyl cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP51153
MaxQBiP51153
PaxDbiP51153
PeptideAtlasiP51153
PRIDEiP51153
ProteomicsDBi56286

PTM databases

iPTMnetiP51153
PhosphoSitePlusiP51153
SwissPalmiP51153

Expressioni

Tissue specificityi

Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.

Inductioni

Up-regulated during osteoclast differentiation.1 Publication

Gene expression databases

BgeeiENSG00000143545 Expressed in 97 organ(s), highest expression level in cortex of kidney
CleanExiHS_RAB13
ExpressionAtlasiP51153 baseline and differential
GenevisibleiP51153 HS

Organism-specific databases

HPAiHPA003996

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL2; competes with RAB8A and is involved in tight junctions assembly. Interacts (GTP-bound form) with MICALL1. Interacts (GTP-bound form) with MICAL1, MICAL3, MICALCL, EHBP1 and EHBP1L1; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible. Interacts with PRKACA; downstream effector of RAB13 involved in tight junction assembly. Interacts with GRB2; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA. Interacts (isoprenylated form) with PDE6D; dissociates RAB13 from membranes. Interacts with BICDL2/BICDR2. Interacts with LEPROT and LEPROTL1.6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi111810, 23 interactors
DIPiDIP-40268N
IntActiP51153, 14 interactors
MINTiP51153
STRINGi9606.ENSP00000357564

Structurei

3D structure databases

ProteinModelPortaliP51153
SMRiP51153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOVERGENiHBG009351
InParanoidiP51153
KOiK06109
OMAiENFFLAW
OrthoDBiEOG091G0LA6
PhylomeDBiP51153
TreeFamiTF314097

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P51153-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV
60 70 80 90 100
DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ
110 120 130 140 150
NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET
160 170 180 190 200
SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC

SLG
Length:203
Mass (Da):22,774
Last modified:October 1, 1996 - v1
Checksum:i141621CB998178DA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WWB9A0A087WWB9_HUMAN
Ras-related protein Rab-13
RAB13
122Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75593 mRNA Translation: CAA53266.1
AY423722 mRNA Translation: AAS00485.1
AF498948 mRNA Translation: AAM21096.1
AK291580 mRNA Translation: BAF84269.1
BT019700 mRNA Translation: AAV38506.1
BT019701 mRNA Translation: AAV38507.1
AL358472 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53249.1
CH471121 Genomic DNA Translation: EAW53250.1
BC000799 mRNA Translation: AAH00799.1
BC009227 mRNA Translation: AAH09227.2
BC073168 mRNA Translation: AAH73168.2
CCDSiCCDS1058.1
PIRiA49647
RefSeqiNP_001258967.1, NM_001272038.1
NP_002861.1, NM_002870.3
UniGeneiHs.151536

Genome annotation databases

EnsembliENST00000368575; ENSP00000357564; ENSG00000143545
GeneIDi5872
KEGGihsa:5872
UCSCiuc001fdt.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75593 mRNA Translation: CAA53266.1
AY423722 mRNA Translation: AAS00485.1
AF498948 mRNA Translation: AAM21096.1
AK291580 mRNA Translation: BAF84269.1
BT019700 mRNA Translation: AAV38506.1
BT019701 mRNA Translation: AAV38507.1
AL358472 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW53249.1
CH471121 Genomic DNA Translation: EAW53250.1
BC000799 mRNA Translation: AAH00799.1
BC009227 mRNA Translation: AAH09227.2
BC073168 mRNA Translation: AAH73168.2
CCDSiCCDS1058.1
PIRiA49647
RefSeqiNP_001258967.1, NM_001272038.1
NP_002861.1, NM_002870.3
UniGeneiHs.151536

3D structure databases

ProteinModelPortaliP51153
SMRiP51153
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111810, 23 interactors
DIPiDIP-40268N
IntActiP51153, 14 interactors
MINTiP51153
STRINGi9606.ENSP00000357564

PTM databases

iPTMnetiP51153
PhosphoSitePlusiP51153
SwissPalmiP51153

Polymorphism and mutation databases

DMDMi1710016

Proteomic databases

EPDiP51153
MaxQBiP51153
PaxDbiP51153
PeptideAtlasiP51153
PRIDEiP51153
ProteomicsDBi56286

Protocols and materials databases

DNASUi5872
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368575; ENSP00000357564; ENSG00000143545
GeneIDi5872
KEGGihsa:5872
UCSCiuc001fdt.3 human

Organism-specific databases

CTDi5872
DisGeNETi5872
EuPathDBiHostDB:ENSG00000143545.8
GeneCardsiRAB13
HGNCiHGNC:9762 RAB13
HPAiHPA003996
MIMi602672 gene
neXtProtiNX_P51153
OpenTargetsiENSG00000143545
PharmGKBiPA34103
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOVERGENiHBG009351
InParanoidiP51153
KOiK06109
OMAiENFFLAW
OrthoDBiEOG091G0LA6
PhylomeDBiP51153
TreeFamiTF314097

Enzyme and pathway databases

ReactomeiR-HSA-1445148 Translocation of SLC2A4 (GLUT4) to the plasma membrane
R-HSA-8873719 RAB geranylgeranylation
R-HSA-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiRAB13 human
GeneWikiiRAB13
GenomeRNAii5872
PROiPR:P51153
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143545 Expressed in 97 organ(s), highest expression level in cortex of kidney
CleanExiHS_RAB13
ExpressionAtlasiP51153 baseline and differential
GenevisibleiP51153 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB13_HUMAN
AccessioniPrimary (citable) accession number: P51153
Secondary accession number(s): A8K6B5
, D3DV67, Q5U0A6, Q6GPG6, Q96GU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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