UniProtKB - P51150 (RAB7A_MOUSE)
Ras-related protein Rab-7a
Rab7a
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 22 | GTPBy similarity | 8 | |
Nucleotide bindingi | 34 – 40 | GTPBy similarity | 7 | |
Nucleotide bindingi | 63 – 67 | GTPBy similarity | 5 | |
Nucleotide bindingi | 125 – 128 | GTPBy similarity | 4 | |
Nucleotide bindingi | 156 – 157 | GTPBy similarity | 2 |
GO - Molecular functioni
- GDP binding Source: MGI
- GTPase activity Source: MGI
- GTP binding Source: MGI
- Rac GTPase binding Source: MGI
- retromer complex binding Source: MGI
GO - Biological processi
- autophagosome assembly Source: MGI
- bone resorption Source: MGI
- early endosome to late endosome transport Source: UniProtKB
- endosome to lysosome transport Source: MGI
- epidermal growth factor catabolic process Source: MGI
- intracellular protein transport Source: MGI
- lipid catabolic process Source: UniProtKB-KW
- lipophagy Source: GO_Central
- negative regulation of exosomal secretion Source: MGI
- phagosome acidification Source: UniProtKB
- phagosome-lysosome fusion Source: UniProtKB
- positive regulation of exosomal secretion Source: MGI
- positive regulation of protein catabolic process Source: MGI
- positive regulation of viral process Source: MGI
- protein targeting to lysosome Source: MGI
- protein to membrane docking Source: MGI
- retrograde transport, endosome to Golgi Source: MGI
- viral release from host cell Source: MGI
Keywordsi
Biological process | Autophagy, Lipid degradation, Lipid metabolism, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-6798695, Neutrophil degranulation R-MMU-8854214, TBC/RABGAPs R-MMU-8873719, RAB geranylgeranylation R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-7a |
Gene namesi | Name:Rab7a Synonyms:Rab7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105068, Rab7 |
Subcellular locationi
Lysosome
- Lysosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
Endosome
- Late endosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
- Endosome membrane By similarity
Other locations
- phagosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
- Melanosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
- autophagosome membrane By similarity; Peripheral membrane protein Curated; Cytoplasmic side Curated
- Lipid droplet 1 Publication
- Cytoplasmic vesicle 1 Publication
Note: Colocalizes with OSBPL1A at the late endosome (By similarity). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity). Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity). Lipid droplet localization is increased upon ADRB2 stimulation (PubMed:23708524).By similarity1 Publication
Cytosol
- cytosol Source: GOC
Endosome
- endosome Source: MGI
- endosome membrane Source: MGI
- late endosome Source: MGI
- late endosome membrane Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: MGI
Lysosome
- extrinsic component of lysosome membrane Source: GO_Central
- lysosome Source: MGI
Other locations
- alveolar lamellar body Source: MGI
- anchored component of synaptic vesicle membrane Source: MGI
- autophagosome membrane Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
- lipid droplet Source: GO_Central
- melanosome membrane Source: UniProtKB-SubCell
- phagocytic vesicle Source: UniProtKB
- phagocytic vesicle membrane Source: UniProtKB-SubCell
- phagophore assembly site membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasmic vesicle, Endosome, Lipid droplet, Lysosome, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000121122 | 2 – 207 | Ras-related protein Rab-7aAdd BLAST | 206 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineBy similarity | 1 | |
Modified residuei | 72 | PhosphoserineCombined sources | 1 | |
Lipidationi | 205 | S-geranylgeranyl cysteineBy similarity | 1 | |
Modified residuei | 207 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 207 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | P51150 |
jPOSTi | P51150 |
PaxDbi | P51150 |
PeptideAtlasi | P51150 |
PRIDEi | P51150 |
PTM databases
iPTMneti | P51150 |
PhosphoSitePlusi | P51150 |
SwissPalmi | P51150 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000079477, Expressed in secondary oocyte and 317 other tissues |
ExpressionAtlasi | P51150, baseline and differential |
Genevisiblei | P51150, MM |
Interactioni
Subunit structurei
Interacts with NTRK1/TRKA (By similarity), RILP (By similarity), PSMA7 (By similarity), RNF115 (PubMed:12972561) and FYCO1 (By similarity).
Interacts with the PIK3C3/VPS34-PIK3R4 complex (By similarity). The GTP-bound form interacts with OSBPL1A and RAC1 (By similarity).
Interacts with CLN3 (By similarity).
Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity).
Interacts with C9orf72 (PubMed:24549040). Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159).
Interacts with CLN5 (By similarity).
Interacts with PLEKHM1 (via N- and C-terminus) (PubMed:27777970).
Interacts with RUFY4 (By similarity).
Interacts with PRPH; the interaction is direct (PubMed:23179371).
Interacts with VPS13A (By similarity).
By similarity5 PublicationsGO - Molecular functioni
- Rac GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202553, 43 interactors |
DIPi | DIP-60513N |
IntActi | P51150, 15 interactors |
MINTi | P51150 |
STRINGi | 10090.ENSMUSP00000109230 |
Miscellaneous databases
RNActi | P51150, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P51150 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 37 – 45 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0394, Eukaryota |
GeneTreei | ENSGT00940000155864 |
HOGENOMi | CLU_041217_10_6_1 |
InParanoidi | P51150 |
OMAi | DGDNCAC |
OrthoDBi | 1172019at2759 |
PhylomeDBi | P51150 |
TreeFami | TF105605 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV
60 70 80 90 100
MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD
110 120 130 140 150
SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP
160 170 180 190 200
YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA
SAESCSC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SVG9 | A0A0N4SVG9_MOUSE | RAB7, member RAS oncogene family | Rab7 | 98 | Annotation score: | ||
A0A0N4SVR6 | A0A0N4SVR6_MOUSE | RAB7, member RAS oncogene family | Rab7 | 88 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 145 | S → R in CAA61797 (PubMed:8547311).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89650 mRNA Translation: CAA61797.1 AK005004 mRNA Translation: BAB23738.1 BC004597 mRNA Translation: AAH04597.1 BC086793 mRNA Translation: AAH86793.1 |
CCDSi | CCDS39552.1 |
PIRi | S62733 |
RefSeqi | NP_001280581.1, NM_001293652.1 NP_001280582.1, NM_001293653.1 NP_001280583.1, NM_001293654.1 NP_001280584.1, NM_001293655.1 NP_033031.2, NM_009005.3 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89650 mRNA Translation: CAA61797.1 AK005004 mRNA Translation: BAB23738.1 BC004597 mRNA Translation: AAH04597.1 BC086793 mRNA Translation: AAH86793.1 |
CCDSi | CCDS39552.1 |
PIRi | S62733 |
RefSeqi | NP_001280581.1, NM_001293652.1 NP_001280582.1, NM_001293653.1 NP_001280583.1, NM_001293654.1 NP_001280584.1, NM_001293655.1 NP_033031.2, NM_009005.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5Z2M | X-ray | 2.14 | A/C | 1-176 | [»] | |
SMRi | P51150 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202553, 43 interactors |
DIPi | DIP-60513N |
IntActi | P51150, 15 interactors |
MINTi | P51150 |
STRINGi | 10090.ENSMUSP00000109230 |
PTM databases
iPTMneti | P51150 |
PhosphoSitePlusi | P51150 |
SwissPalmi | P51150 |
Proteomic databases
EPDi | P51150 |
jPOSTi | P51150 |
PaxDbi | P51150 |
PeptideAtlasi | P51150 |
PRIDEi | P51150 |
Protocols and materials databases
ABCDi | P51150, 21 sequenced antibodies |
Antibodypediai | 1885, 349 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 19349 |
MGIi | MGI:105068, Rab7 |
Phylogenomic databases
eggNOGi | KOG0394, Eukaryota |
GeneTreei | ENSGT00940000155864 |
HOGENOMi | CLU_041217_10_6_1 |
InParanoidi | P51150 |
OMAi | DGDNCAC |
OrthoDBi | 1172019at2759 |
PhylomeDBi | P51150 |
TreeFami | TF105605 |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-6798695, Neutrophil degranulation R-MMU-8854214, TBC/RABGAPs R-MMU-8873719, RAB geranylgeranylation R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
BioGRID-ORCSi | 19349, 10 hits in 21 CRISPR screens |
ChiTaRSi | Rab7, mouse |
PROi | PR:P51150 |
RNActi | P51150, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000079477, Expressed in secondary oocyte and 317 other tissues |
ExpressionAtlasi | P51150, baseline and differential |
Genevisiblei | P51150, MM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB7A_MOUSE | |
Accessioni | P51150Primary (citable) accession number: P51150 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | April 13, 2004 | |
Last modified: | December 2, 2020 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families