UniProtKB - P51003 (PAPOA_HUMAN)
Poly(A) polymerase alpha
PAPOLA
Functioni
Catalytic activityi
- EC:2.7.7.19
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | ATPBy similarity | 1 | |
Metal bindingi | 113 | Magnesium 1; catalyticBy similarity | 1 | |
Metal bindingi | 113 | Magnesium 2; catalyticBy similarity | 1 | |
Metal bindingi | 115 | Magnesium 1; catalyticBy similarity | 1 | |
Metal bindingi | 115 | Magnesium 2; catalyticBy similarity | 1 | |
Metal bindingi | 167 | Magnesium 2; catalyticBy similarity | 1 | |
Binding sitei | 167 | ATPBy similarity | 1 | |
Binding sitei | 228 | ATPBy similarity | 1 | |
Binding sitei | 237 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 100 – 102 | ATPBy similarity | 3 | |
Nucleotide bindingi | 113 – 115 | ATPBy similarity | 3 | |
Nucleotide bindingi | 246 – 247 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- manganese ion binding Source: UniProtKB
- polynucleotide adenylyltransferase activity Source: UniProtKB
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- mRNA 3'-end processing Source: Reactome
- mRNA polyadenylation Source: UniProtKB
- mRNA splicing, via spliceosome Source: Reactome
- regulation of mRNA 3'-end processing Source: UniProtKB
- RNA polyadenylation Source: UniProtKB
- termination of RNA polymerase II transcription Source: Reactome
Keywordsi
Molecular function | RNA-binding, Transferase |
Biological process | mRNA processing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P51003 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination R-HSA-77595, Processing of Intronless Pre-mRNAs |
SABIO-RKi | P51003 |
SIGNORi | P51003 |
Names & Taxonomyi
Protein namesi | Recommended name: Poly(A) polymerase alpha (EC:2.7.7.19)Short name: PAP-alpha Alternative name(s): Polynucleotide adenylyltransferase alpha |
Gene namesi | Name:PAPOLA Synonyms:PAP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000090060.17 |
HGNCi | HGNC:14981, PAPOLA |
MIMi | 605553, gene |
neXtProti | NX_P51003 |
Subcellular locationi
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10914 |
OpenTargetsi | ENSG00000090060 |
PharmGKBi | PA32932 |
Miscellaneous databases
Pharosi | P51003, Tbio |
Chemistry databases
DrugBanki | DB02153, 3-sulfino-L-alanine DB01860, Cordycepin Triphosphate DB03896, Triphosphoric acid |
Polymorphism and mutation databases
BioMutai | PAPOLA |
DMDMi | 59803092 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000051612 | 2 – 745 | Poly(A) polymerase alphaAdd BLAST | 744 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | PhosphoserineCombined sources | 1 | |
Modified residuei | 24 | PhosphoserineCombined sources | 1 | |
Cross-linki | 444 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)Curated | ||
Cross-linki | 445 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)Curated | ||
Cross-linki | 506 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)Curated | ||
Cross-linki | 507 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)Curated | ||
Modified residuei | 537 | PhosphoserineBy similarity | 1 | |
Modified residuei | 558 | PhosphoserineCombined sources | 1 | |
Modified residuei | 641 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 650 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 736 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 736 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Modified residuei | 738 | PhosphoserineCombined sources | 1 | |
Modified residuei | 740 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 740 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P51003 |
jPOSTi | P51003 |
MassIVEi | P51003 |
MaxQBi | P51003 |
PaxDbi | P51003 |
PeptideAtlasi | P51003 |
PRIDEi | P51003 |
ProteomicsDBi | 56276 [P51003-1] 56277 [P51003-2] |
PTM databases
iPTMneti | P51003 |
MetOSitei | P51003 |
PhosphoSitePlusi | P51003 |
Expressioni
Gene expression databases
Bgeei | ENSG00000090060, Expressed in testis and 242 other tissues |
ExpressionAtlasi | P51003, baseline and differential |
Genevisiblei | P51003, HS |
Organism-specific databases
HPAi | ENSG00000090060, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
Found in a complex with CPSF1, FIP1L1 and PAPOLA.
Interacts with AHCYL1 and FIP1L1; the interaction with AHCYL1 seems to increase interaction with FIP1L1 (PubMed:19224921).
Interacts with NUDT21; the interaction is diminished by acetylation.
Interacts with KPNB1; the interaction promotes PAP nuclear import and is inhibited by acetylation of PAP (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 153 | Interaction with RNABy similarity | 1 | |
Sitei | 158 | Interaction with RNABy similarity | 1 | |
Sitei | 328 | Interaction with RNABy similarity | 1 | |
Sitei | 399 | Interaction with RNABy similarity | 1 | |
Sitei | 524 | Interaction with RNABy similarity | 1 |
Protein-protein interaction databases
BioGRIDi | 116119, 55 interactors |
CORUMi | P51003 |
DIPi | DIP-27610N DIP-40865N |
IntActi | P51003, 17 interactors |
MINTi | P51003 |
STRINGi | 9606.ENSP00000216277 |
Miscellaneous databases
RNActi | P51003, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 508 – 643 | Ser/Thr-richAdd BLAST | 136 | |
Regioni | 677 – 745 | Required for interaction with NUDT21Add BLAST | 69 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 490 – 507 | Nuclear localization signal 1By similarityAdd BLAST | 18 | |
Motifi | 650 – 665 | Nuclear localization signal 2By similarityAdd BLAST | 16 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 53 – 57 | Poly-Glu | 5 | |
Compositional biasi | 504 – 507 | Poly-Lys | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2245, Eukaryota |
GeneTreei | ENSGT00940000154598 |
HOGENOMi | CLU_011511_0_0_1 |
InParanoidi | P51003 |
OMAi | MGIVVRH |
PhylomeDBi | P51003 |
TreeFami | TF300842 |
Family and domain databases
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR043519, NT_sf IPR011068, NuclTrfase_I-like_C IPR007012, PolA_pol_cen_dom IPR007010, PolA_pol_RNA-bd_dom IPR014492, PolyA_polymerase IPR002934, Polymerase_NTP_transf_dom |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF04928, PAP_central, 1 hit PF04926, PAP_RNA-bind, 1 hit |
PIRSFi | PIRSF018425, PolyA_polymerase, 1 hit |
SUPFAMi | SSF55003, SSF55003, 1 hit SSF81301, SSF81301, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPFPVTTQGS QQTQPPQKHY GITSPISLAA PKETDCVLTQ KLIETLKPFG
60 70 80 90 100
VFEEEEELQR RILILGKLNN LVKEWIREIS ESKNLPQSVI ENVGGKIFTF
110 120 130 140 150
GSYRLGVHTK GADIDALCVA PRHVDRSDFF TSFYDKLKLQ EEVKDLRAVE
160 170 180 190 200
EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS
210 220 230 240 250
LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKRHNIYSNI LGFLGGVSWA
260 270 280 290 300
MLVARTCQLY PNAIASTLVH KFFLVFSKWE WPNPVLLKQP EECNLNLPVW
310 320 330 340 350
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMVEE FKQGLAITDE
360 370 380 390 400
ILLSKAEWSK LFEAPNFFQK YKHYIVLLAS APTEKQRLEW VGLVESKIRI
410 420 430 440 450
LVGSLEKNEF ITLAHVNPQS FPAPKENPDK EEFRTMWVIG LVFKKTENSE
460 470 480 490 500
NLSVDLTYDI QSFTDTVYRQ AINSKMFEVD MKIAAMHVKR KQLHQLLPNH
510 520 530 540 550
VLQKKKKHST EGVKLTALND SSLDLSMDSD NSMSVPSPTS ATKTSPLNSS
560 570 580 590 600
GSSQGRNSPA PAVTAASVTN IQATEVSVPQ VNSSESSGGT SSESIPQTAT
610 620 630 640 650
QPAISPPPKP TVSRVVSSTR LVNPPPRSSG NAATSGNAAT KIPTPIVGVK
660 670 680 690 700
RTSSPHKEES PKKTKTEEDE TSEDANCLAL SGHDKTEAKE QLDTETSTTQ
710 720 730 740
SETIQTAASL LASQKTSSTD LSDIPALPAN PIPVIKNSIK LRLNR
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3XAH6 | G3XAH6_HUMAN | Poly(A) polymerase | PAPOLA | 724 | Annotation score: | ||
A0A0C4DGK1 | A0A0C4DGK1_HUMAN | Polynucleotide adenylyltransferase | PAPOLA hCG_24516 | 238 | Annotation score: | ||
G3V3I9 | G3V3I9_HUMAN | Polynucleotide adenylyltransferase | PAPOLA | 177 | Annotation score: | ||
G3V457 | G3V457_HUMAN | Polynucleotide adenylyltransferase | PAPOLA | 111 | Annotation score: | ||
H0YJ00 | H0YJ00_HUMAN | Polynucleotide adenylyltransferase | PAPOLA | 203 | Annotation score: | ||
H0YJL4 | H0YJL4_HUMAN | Poly(A) polymerase alpha | PAPOLA | 181 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 – 3 | PF → GTSNSPGHSSFSAPSTKKIK TTRKQNIAWC in CAD62628 (Ref. 2) Curated | 2 | |
Sequence conflicti | 204 – 238 | CRVTD…HNIYS → MRKPTSFCVLQFLSDISCFY TSFVLKLFIAILLTQ in CAD61935 (Ref. 2) CuratedAdd BLAST | 35 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012895 | 280 – 285 | EWPNPV → YVFRLY in isoform 2. 2 Publications | 6 | |
Alternative sequenceiVSP_012896 | 286 – 745 | Missing in isoform 2. 2 PublicationsAdd BLAST | 460 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC000927 mRNA Translation: AAH00927.1 BC036014 mRNA Translation: AAH36014.1 BX248301 mRNA Translation: CAD62628.1 BX248753 mRNA Translation: CAD66560.1 BX161482 mRNA Translation: CAD61935.1 X76770 mRNA No translation available. |
CCDSi | CCDS58334.1 [P51003-2] CCDS9946.1 [P51003-1] |
RefSeqi | NP_001238935.1, NM_001252006.1 [P51003-2] NP_001238936.1, NM_001252007.1 NP_001280556.1, NM_001293627.1 NP_001280557.1, NM_001293628.1 NP_001280561.1, NM_001293632.1 NP_116021.2, NM_032632.4 [P51003-1] |
Genome annotation databases
Ensembli | ENST00000216277; ENSP00000216277; ENSG00000090060 [P51003-1] ENST00000557320; ENSP00000450437; ENSG00000090060 [P51003-2] |
GeneIDi | 10914 |
KEGGi | hsa:10914 |
UCSCi | uc001yfo.5, human [P51003-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC000927 mRNA Translation: AAH00927.1 BC036014 mRNA Translation: AAH36014.1 BX248301 mRNA Translation: CAD62628.1 BX248753 mRNA Translation: CAD66560.1 BX161482 mRNA Translation: CAD61935.1 X76770 mRNA No translation available. |
CCDSi | CCDS58334.1 [P51003-2] CCDS9946.1 [P51003-1] |
RefSeqi | NP_001238935.1, NM_001252006.1 [P51003-2] NP_001238936.1, NM_001252007.1 NP_001280556.1, NM_001293627.1 NP_001280557.1, NM_001293628.1 NP_001280561.1, NM_001293632.1 NP_116021.2, NM_032632.4 [P51003-1] |
3D structure databases
SMRi | P51003 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116119, 55 interactors |
CORUMi | P51003 |
DIPi | DIP-27610N DIP-40865N |
IntActi | P51003, 17 interactors |
MINTi | P51003 |
STRINGi | 9606.ENSP00000216277 |
Chemistry databases
DrugBanki | DB02153, 3-sulfino-L-alanine DB01860, Cordycepin Triphosphate DB03896, Triphosphoric acid |
PTM databases
iPTMneti | P51003 |
MetOSitei | P51003 |
PhosphoSitePlusi | P51003 |
Polymorphism and mutation databases
BioMutai | PAPOLA |
DMDMi | 59803092 |
Proteomic databases
EPDi | P51003 |
jPOSTi | P51003 |
MassIVEi | P51003 |
MaxQBi | P51003 |
PaxDbi | P51003 |
PeptideAtlasi | P51003 |
PRIDEi | P51003 |
ProteomicsDBi | 56276 [P51003-1] 56277 [P51003-2] |
Protocols and materials databases
Antibodypediai | 86, 137 antibodies |
Genome annotation databases
Ensembli | ENST00000216277; ENSP00000216277; ENSG00000090060 [P51003-1] ENST00000557320; ENSP00000450437; ENSG00000090060 [P51003-2] |
GeneIDi | 10914 |
KEGGi | hsa:10914 |
UCSCi | uc001yfo.5, human [P51003-1] |
Organism-specific databases
CTDi | 10914 |
DisGeNETi | 10914 |
EuPathDBi | HostDB:ENSG00000090060.17 |
GeneCardsi | PAPOLA |
HGNCi | HGNC:14981, PAPOLA |
HPAi | ENSG00000090060, Low tissue specificity |
MIMi | 605553, gene |
neXtProti | NX_P51003 |
OpenTargetsi | ENSG00000090060 |
PharmGKBi | PA32932 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2245, Eukaryota |
GeneTreei | ENSGT00940000154598 |
HOGENOMi | CLU_011511_0_0_1 |
InParanoidi | P51003 |
OMAi | MGIVVRH |
PhylomeDBi | P51003 |
TreeFami | TF300842 |
Enzyme and pathway databases
PathwayCommonsi | P51003 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72187, mRNA 3'-end processing R-HSA-73856, RNA Polymerase II Transcription Termination R-HSA-77595, Processing of Intronless Pre-mRNAs |
SABIO-RKi | P51003 |
SIGNORi | P51003 |
Miscellaneous databases
BioGRID-ORCSi | 10914, 134 hits in 846 CRISPR screens |
ChiTaRSi | PAPOLA, human |
GeneWikii | PAPOLA |
GenomeRNAii | 10914 |
Pharosi | P51003, Tbio |
PROi | PR:P51003 |
RNActi | P51003, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000090060, Expressed in testis and 242 other tissues |
ExpressionAtlasi | P51003, baseline and differential |
Genevisiblei | P51003, HS |
Family and domain databases
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR043519, NT_sf IPR011068, NuclTrfase_I-like_C IPR007012, PolA_pol_cen_dom IPR007010, PolA_pol_RNA-bd_dom IPR014492, PolyA_polymerase IPR002934, Polymerase_NTP_transf_dom |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF04928, PAP_central, 1 hit PF04926, PAP_RNA-bind, 1 hit |
PIRSFi | PIRSF018425, PolyA_polymerase, 1 hit |
SUPFAMi | SSF55003, SSF55003, 1 hit SSF81301, SSF81301, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAPOA_HUMAN | |
Accessioni | P51003Primary (citable) accession number: P51003 Secondary accession number(s): Q86SX4 Q9BVU2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM