UniProtKB - P50997 (AT1A1_CANLF)
Sodium/potassium-transporting ATPase subunit alpha-1
ATP1A1
Functioni
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
By similarityCatalytic activityi
- EC:7.2.2.13
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 374 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Binding sitei | 485 | ATPBy similarity | 1 | |
Metal bindingi | 715 | MagnesiumBy similarity | 1 | |
Metal bindingi | 719 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- P-type sodium:potassium-exchanging transporter activity Source: UniProtKB
GO - Biological processi
- regulation of sodium ion transport Source: UniProtKB
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/potassium-transporting ATPase subunit alpha-1 (EC:7.2.2.13)Short name: Na(+)/K(+) ATPase alpha-1 subunit Alternative name(s): Sodium pump subunit alpha-1 |
Gene namesi | Name:ATP1A1 |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- sarcolemma By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- axon By similarity
- Melanosome By similarity
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- sarcolemma Source: UniProtKB-SubCell
Other locations
- axon Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB
- melanosome Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 4 – 85 | CytoplasmicSequence analysisAdd BLAST | 82 | |
Transmembranei | 86 – 106 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 107 – 129 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 130 – 150 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 151 – 286 | CytoplasmicSequence analysisAdd BLAST | 136 | |
Transmembranei | 287 – 306 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 307 – 318 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 319 – 336 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 337 – 770 | CytoplasmicSequence analysisAdd BLAST | 434 | |
Transmembranei | 771 – 790 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 791 – 800 | ExtracellularSequence analysis | 10 | |
Transmembranei | 801 – 821 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 822 – 841 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 842 – 864 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 865 – 916 | ExtracellularSequence analysisAdd BLAST | 52 | |
Transmembranei | 917 – 936 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 937 – 949 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 950 – 968 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 969 – 983 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 984 – 1004 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1005 – 1021 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000002479 | 1 – 5 | By similarity | 5 | |
ChainiPRO_0000002480 | 6 – 1021 | Sodium/potassium-transporting ATPase subunit alpha-1Add BLAST | 1016 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 10 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 16 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 21 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 38 | PhosphoserineBy similarity | 1 | |
Modified residuei | 45 | PhosphoserineBy similarity | 1 | |
Modified residuei | 226 | PhosphoserineBy similarity | 1 | |
Modified residuei | 258 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 450 | PhosphoserineBy similarity | 1 | |
Modified residuei | 482 | PhosphoserineBy similarity | 1 | |
Modified residuei | 540 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 659 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 666 | PhosphoserineBy similarity | 1 | |
Modified residuei | 673 | PhosphoserineBy similarity | 1 | |
Modified residuei | 941 | Phosphoserine; by PKABy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P50997 |
Interactioni
Subunit structurei
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
Interacts with regulatory subunit FXYD1 (PubMed:16943195, PubMed:21454534).
Interacts with regulatory subunit FXYD3 (By similarity).
Interacts with SIK1 (By similarity).
Interacts with SLC35G1 and STIM1 (By similarity).
Interacts with CLN3; this interaction regulates the sodium/potassium-transporting ATPase complex localization at the plasma membrane (By similarity).
By similarity2 PublicationsProtein-protein interaction databases
STRINGi | 9612.ENSCAFP00000038629 |
Chemistry databases
BindingDBi | P50997 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 36 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 80 – 82 | Phosphoinositide-3 kinase bindingBy similarity | 3 | |
Regioni | 214 – 233 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0203, Eukaryota |
InParanoidi | P50997 |
OrthoDBi | 100699at2759 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGKGVGRDKY EPAAVSEHGD KKKAKKERDM DELKKEVSMD DHKLSLDELH
60 70 80 90 100
RKYGTDLSRG LTTARAAEIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSM
110 120 130 140 150
LLWIGAILCF LAYGIQAATE EEPQNDNLYL GVVLSAVVII TGCFSYYQEA
160 170 180 190 200
KSSKIMESFK NMVPQQALVI RNGEKMSINA EEVVIGDLVE VKGGDRIPAD
210 220 230 240 250
LRIISANGCK VDNSSLTGES EPQTRSPDFT NENPLETRNI AFFSTNCVKG
260 270 280 290 300
TARGIVVYTG DRTVMGRIAT LASGLEGGQT PIAAEIEHFI HIITGVAVFL
310 320 330 340 350
GVSFFILSLI LEYTWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TENQSGVSFD KSSATWLALS RIAGLCNRAV FQANQENLPI LKRAVAGDAS
460 470 480 490 500
ESALLKCIEL CCGSVKEMRD RYAKIVEIPF NSTNKYQLSI HKNPNTSEPR
510 520 530 540 550
HLLVMKGAPE RILDRCSSIL LHGKEQPLDE ELKDALQNAY LELGGLGERV
560 570 580 590 600
LGFRHLFLPD EQFPEGFQFD TDDVNFPVEN LCFVGFISMI GPPRAAVPDA
610 620 630 640 650
VGKCRGAGIK VIMVTGDHPI TAKAIAKGAG IISEGNETVE DIAARLNIPV
660 670 680 690 700
RQVNPRDAKA CVVHGSDLKD MTSEQLDGIL KYHTEIVFAR TSPQQKLIIV
710 720 730 740 750
EGCQRQGAIV AVTGDGVNDS PALKKADIGV AMGIVGSDAS KQAADMILLD
760 770 780 790 800
DNFASIVTGV EEGRLIFDNL KKSIAYTLTS NIPEITPFLI FIIANIPLPL
810 820 830 840 850
GTVTILCIDL GTDMVPAISL AYEQAESDIM KRQPRNPKTD KLVNERLISM
860 870 880 890 900
AYGQIGMIQA LGGFFTYFVI LAENGFLPTH LLGLRVDWDD RWINDVEDSY
910 920 930 940 950
GQQWTYEQRK IVEFTCHTAF FVSIVVVQWA DLVICKTRRN SVFQQGMKNK
960 970 980 990 1000
ILIFGLFEET ALAAFLSYCP GMGVALRMYP LKPTWWFCAF PYSLLIFVYD
1010 1020
EVRKLIIRRR PGGWVEKETY Y
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 109 | C → Y in CAA46950 (PubMed:1316269).Curated | 1 | |
Sequence conflicti | 185 | I → V in CAA46950 (PubMed:1316269).Curated | 1 | |
Sequence conflicti | 249 | K → E in CAA46950 (PubMed:1316269).Curated | 1 | |
Sequence conflicti | 309 | L → F in CAA46950 (PubMed:1316269).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42173 mRNA Translation: AAA67372.1 X66174 mRNA Translation: CAA46950.1 |
RefSeqi | NP_001003306.1, NM_001003306.2 |
Genome annotation databases
GeneIDi | 403992 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42173 mRNA Translation: AAA67372.1 X66174 mRNA Translation: CAA46950.1 |
RefSeqi | NP_001003306.1, NM_001003306.2 |
3D structure databases
AlphaFoldDBi | P50997 |
SMRi | P50997 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000038629 |
Chemistry databases
BindingDBi | P50997 |
ChEMBLi | CHEMBL4838 |
DrugCentrali | P50997 |
Proteomic databases
PaxDbi | P50997 |
Genome annotation databases
GeneIDi | 403992 |
Organism-specific databases
CTDi | 476 |
Phylogenomic databases
eggNOGi | KOG0203, Eukaryota |
InParanoidi | P50997 |
OrthoDBi | 100699at2759 |
Miscellaneous databases
PROi | PR:P50997 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT1A1_CANLF | |
Accessioni | P50997Primary (citable) accession number: P50997 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families