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UniProtKB - P50866 (CLPX_BACSU)
Protein
ATP-dependent Clp protease ATP-binding subunit ClpX
Gene
clpX
Organism
Bacillus subtilis (strain 168)
Status
Functioni
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity).
Probably the major protease that degrades proteins tagged by trans-translation (PubMed:11395451).
UniRule annotation1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 13 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 16 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 35 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 38 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 117 – 124 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: GO_Central
- ATP hydrolysis activity Source: GO_Central
- protein dimerization activity Source: InterPro
- unfolded protein binding Source: UniProtKB-UniRule
- zinc ion binding Source: InterPro
GO - Biological processi
- cell division Source: GO_Central
- protein catabolic process Source: CACAO
- protein folding Source: UniProtKB-UniRule
- proteolysis Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU28220-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent Clp protease ATP-binding subunit ClpXUniRule annotation |
Gene namesi | Name:clpXUniRule annotation Ordered Locus Names:BSU28220 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
No degradation of trans-translationally tagged-peptides.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160313 | 1 – 420 | ATP-dependent Clp protease ATP-binding subunit ClpXAdd BLAST | 420 |
Proteomic databases
jPOSTi | P50866 |
PaxDbi | P50866 |
PRIDEi | P50866 |
Expressioni
Inductioni
By heat shock.1 Publication
Interactioni
Subunit structurei
Component of the ClpX-ClpP complex.
Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.
UniRule annotationGO - Molecular functioni
- protein dimerization activity Source: InterPro
- unfolded protein binding Source: UniProtKB-UniRule
Protein-protein interaction databases
IntActi | P50866, 1 interactor |
MINTi | P50866 |
STRINGi | 224308.BSU28220 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 54 | ClpX-type ZBPROSITE-ProRule annotationAdd BLAST | 54 |
Sequence similaritiesi
Belongs to the ClpX chaperone family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1219, Bacteria |
InParanoidi | P50866 |
OMAi | HYKRVQA |
PhylomeDBi | P50866 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit 6.20.220.10, 1 hit |
HAMAPi | MF_00175, ClpX, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR019489, Clp_ATPase_C IPR004487, Clp_protease_ATP-bd_su_ClpX IPR027417, P-loop_NTPase IPR010603, Znf_CppX_C4 IPR038366, Znf_CppX_C4_sf |
Pfami | View protein in Pfam PF07724, AAA_2, 1 hit PF10431, ClpB_D2-small, 1 hit PF06689, zf-C4_ClpX, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM01086, ClpB_D2-small, 1 hit SM00994, zf-C4_ClpX, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00382, clpX, 1 hit |
PROSITEi | View protein in PROSITE PS51902, CLPX_ZB, 1 hit |
i Sequence
Sequence statusi: Complete.
P50866-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKFNEEKGQ LKCSFCGKTQ DQVRKLVAGP GVYICDECIE LCTEIVEEEL
60 70 80 90 100
GTEEEVEFKD VPKPQEIREI LNEYVIGQDQ AKKSLAVAVY NHYKRINSNS
110 120 130 140 150
KVDDVELSKS NISLIGPTGS GKTLLAQTLA RILNVPFAIA DATSLTEAGY
160 170 180 190 200
VGEDVENILL KLIQAADYDV EKAEKGIIYI DEIDKVARKS ENPSITRDVS
210 220 230 240 250
GEGVQQALLK ILEGTVASVP PQGGRKHPHQ EFIQIDTTNI LFICGGAFDG
260 270 280 290 300
IEQIIKRRLG QKVIGFGADN KAADLEKEDL LSKVLPEDLL RFGLIPEFIG
310 320 330 340 350
RLPVIASLEK LDEEALVAIL TKPKNALVKQ FKKMLELDNV ELEFEEEALS
360 370 380 390 400
EIAKKAIERK TGARGLRSII EGIMLDVMFE LPSRDDIEKC VITGATVTHG
410 420
EPPRLLLKDG TEVSQDKTSA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 109 | K → Q in CAA64618 (PubMed:8973311).Curated | 1 | |
Sequence conflicti | 109 | K → Q in CAA99537 (PubMed:8969504).Curated | 1 | |
Sequence conflicti | 161 | K → E in CAA64618 (PubMed:8973311).Curated | 1 | |
Sequence conflicti | 161 | K → E in CAA99537 (PubMed:8969504).Curated | 1 | |
Sequence conflicti | 173 – 174 | AE → TG in CAA64618 (PubMed:8973311).Curated | 2 | |
Sequence conflicti | 173 – 174 | AE → TG in CAA99537 (PubMed:8969504).Curated | 2 | |
Sequence conflicti | 247 | A → R in CAA99537 (PubMed:8969504).Curated | 1 | |
Sequence conflicti | 247 | A → R in AAA84743 (Ref. 5) Curated | 1 | |
Sequence conflicti | 260 | G → A in CAA99537 (PubMed:8969504).Curated | 1 | |
Sequence conflicti | 260 | G → A in AAA84743 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95306 Genomic DNA Translation: CAA64618.1 Z75208 Genomic DNA Translation: CAA99537.1 AL009126 Genomic DNA Translation: CAB14782.2 U18229 Genomic DNA Translation: AAA84743.1 |
PIRi | D69601 |
RefSeqi | NP_390700.2, NC_000964.3 WP_003229613.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14782; CAB14782; BSU_28220 |
GeneIDi | 937482 |
KEGGi | bsu:BSU28220 |
PATRICi | fig|224308.179.peg.3065 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95306 Genomic DNA Translation: CAA64618.1 Z75208 Genomic DNA Translation: CAA99537.1 AL009126 Genomic DNA Translation: CAB14782.2 U18229 Genomic DNA Translation: AAA84743.1 |
PIRi | D69601 |
RefSeqi | NP_390700.2, NC_000964.3 WP_003229613.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P50866 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P50866, 1 interactor |
MINTi | P50866 |
STRINGi | 224308.BSU28220 |
Proteomic databases
jPOSTi | P50866 |
PaxDbi | P50866 |
PRIDEi | P50866 |
Genome annotation databases
EnsemblBacteriai | CAB14782; CAB14782; BSU_28220 |
GeneIDi | 937482 |
KEGGi | bsu:BSU28220 |
PATRICi | fig|224308.179.peg.3065 |
Phylogenomic databases
eggNOGi | COG1219, Bacteria |
InParanoidi | P50866 |
OMAi | HYKRVQA |
PhylomeDBi | P50866 |
Enzyme and pathway databases
BioCyci | BSUB:BSU28220-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit 6.20.220.10, 1 hit |
HAMAPi | MF_00175, ClpX, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR019489, Clp_ATPase_C IPR004487, Clp_protease_ATP-bd_su_ClpX IPR027417, P-loop_NTPase IPR010603, Znf_CppX_C4 IPR038366, Znf_CppX_C4_sf |
Pfami | View protein in Pfam PF07724, AAA_2, 1 hit PF10431, ClpB_D2-small, 1 hit PF06689, zf-C4_ClpX, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM01086, ClpB_D2-small, 1 hit SM00994, zf-C4_ClpX, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00382, clpX, 1 hit |
PROSITEi | View protein in PROSITE PS51902, CLPX_ZB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CLPX_BACSU | |
Accessioni | P50866Primary (citable) accession number: P50866 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 7, 2009 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families