UniProtKB - P50854 (RISA_ACTPL)
Protein
Riboflavin synthase
Gene
ribE
Organism
Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Status
Functioni
Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.By similarity
Catalytic activityi
- EC:2.5.1.9
: riboflavin biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- 6,7-dimethyl-8-ribityllumazine synthase (ribH), 6,7-dimethyl-8-ribityllumazine synthase (ribH_1), 6,7-dimethyl-8-ribityllumazine synthase (ribH), 6,7-dimethyl-8-ribityllumazine synthase (ribH_2), 6,7-dimethyl-8-ribityllumazine synthase (ribH)
- Riboflavin synthase (ribE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 135 | Lumazine 2By similarity | 1 |
GO - Molecular functioni
- riboflavin synthase activity Source: UniProtKB-EC
GO - Biological processi
- riboflavin biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Riboflavin biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00405 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ribE Synonyms:ribB |
Organismi | Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae) |
Taxonomic identifieri | 715 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068156 | 1 – 215 | Riboflavin synthaseAdd BLAST | 215 |
Interactioni
Subunit structurei
Homotrimer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 1 – 96 | Lumazine-binding 1Add BLAST | 96 | |
Repeati | 97 – 193 | Lumazine-binding 2Add BLAST | 97 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 4 – 6 | Lumazine 1 bindingBy similarity | 3 | |
Regioni | 47 – 49 | Lumazine 2 binding; shared with one trimeric partnerBy similarity | 3 | |
Regioni | 61 – 66 | Lumazine 2 binding; shared with one trimeric partnerBy similarity | 6 | |
Regioni | 100 – 102 | Lumazine 2 binding; shared with one trimeric partnerBy similarity | 3 | |
Regioni | 144 – 146 | Lumazine 1 bindingBy similarity | 3 | |
Regioni | 158 – 163 | Lumazine 1 bindingBy similarity | 6 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG4108R6K Bacteria COG0307 LUCA |
Family and domain databases
CDDi | cd00402 Riboflavin_synthase_like, 1 hit |
Gene3Di | 2.40.30.20, 2 hits |
InterProi | View protein in InterPro IPR023366 ATP_synth_asu-like_sf IPR001783 Lumazine-bd IPR026017 Lumazine-bd_dom IPR017938 Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR21098 PTHR21098, 1 hit |
Pfami | View protein in Pfam PF00677 Lum_binding, 2 hits |
PIRSFi | PIRSF000498 Riboflavin_syn_A, 1 hit |
SUPFAMi | SSF63380 SSF63380, 2 hits |
TIGRFAMsi | TIGR00187 ribE, 1 hit |
PROSITEi | View protein in PROSITE PS51177 LUMAZINE_BIND, 2 hits |
i Sequence
Sequence statusi: Complete.
P50854-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFTGIIEEVG KIAQIHKQGE FAVVTINATK VLQDVHLGDT IAVNGVCLTV
60 70 80 90 100
TSFSSNQFTA DVMSETLKRT SLGELKSNSP VNLERAMAAN GRFGGHIVSG
110 120 130 140 150
HIDGTGEIAE ITPAHNSTWY RIKTSPKLMR YIIEKGSITI DGISLTVVDT
160 170 180 190 200
DDESFRVSII PHTIKETNLG SKKIGSIVNL ENDIVGKYIE QFLLKKPADE
210
PKSNLSLDFL KQAGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27202 Genomic DNA Translation: AAA86523.1 |
PIRi | T50547 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27202 Genomic DNA Translation: AAA86523.1 |
PIRi | T50547 |
3D structure databases
SMRi | P50854 |
ModBasei | Search... |
Phylogenomic databases
eggNOGi | ENOG4108R6K Bacteria COG0307 LUCA |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00405 |
Family and domain databases
CDDi | cd00402 Riboflavin_synthase_like, 1 hit |
Gene3Di | 2.40.30.20, 2 hits |
InterProi | View protein in InterPro IPR023366 ATP_synth_asu-like_sf IPR001783 Lumazine-bd IPR026017 Lumazine-bd_dom IPR017938 Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR21098 PTHR21098, 1 hit |
Pfami | View protein in Pfam PF00677 Lum_binding, 2 hits |
PIRSFi | PIRSF000498 Riboflavin_syn_A, 1 hit |
SUPFAMi | SSF63380 SSF63380, 2 hits |
TIGRFAMsi | TIGR00187 ribE, 1 hit |
PROSITEi | View protein in PROSITE PS51177 LUMAZINE_BIND, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RISA_ACTPL | |
Accessioni | P50854Primary (citable) accession number: P50854 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 8, 2019 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |