UniProtKB - P50637 (TSPO_MOUSE)
Protein
Translocator protein
Gene
Tspo
Organism
Mus musculus (Mouse)
Status
Functioni
Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:9832438, PubMed:24814875), but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis (PubMed:24174323, PubMed:24936060).By similarity5 Publications
GO - Molecular functioni
- androgen binding Source: MGI
- benzodiazepine receptor activity Source: MGI
- cholesterol binding Source: GO_Central
- ion channel binding Source: ParkinsonsUK-UCL
GO - Biological processi
- adrenal gland development Source: Ensembl
- aging Source: Ensembl
- behavioral response to pain Source: MGI
- cellular hypotonic response Source: Ensembl
- cellular response to lipopolysaccharide Source: Ensembl
- cellular response to zinc ion Source: Ensembl
- chloride transport Source: MGI
- contact inhibition Source: MGI
- establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
- glial cell migration Source: MGI
- ion transport Source: MGI
- lipid transport Source: UniProtKB-KW
- maintenance of protein location in mitochondrion Source: ParkinsonsUK-UCL
- negative regulation of ATP metabolic process Source: ParkinsonsUK-UCL
- negative regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
- negative regulation of glial cell proliferation Source: MGI
- negative regulation of mitochondrion organization Source: ParkinsonsUK-UCL
- negative regulation of nitric oxide biosynthetic process Source: MGI
- negative regulation of protein ubiquitination Source: ParkinsonsUK-UCL
- negative regulation of tumor necrosis factor production Source: MGI
- peripheral nervous system axon regeneration Source: Ensembl
- positive regulation of apoptotic process Source: MGI
- positive regulation of calcium ion transport Source: MGI
- positive regulation of glial cell proliferation Source: MGI
- positive regulation of mitochondrial depolarization Source: MGI
- positive regulation of necrotic cell death Source: MGI
- positive regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
- regulation of steroid biosynthetic process Source: MGI
- response to drug Source: Ensembl
- response to manganese ion Source: Ensembl
- response to progesterone Source: Ensembl
- response to testosterone Source: Ensembl
- response to vitamin B1 Source: Ensembl
- steroid biosynthetic process Source: MGI
Keywordsi
Molecular function | Receptor |
Biological process | Lipid transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-196108, Pregnenolone biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Translocator proteinAlternative name(s): Mitochondrial benzodiazepine receptor PKBS Peripheral-type benzodiazepine receptor Short name: PBR |
Gene namesi | Name:Tspo Synonyms:Bzrp, Mbr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88222, Tspo |
Subcellular locationi
Mitochondrion
- Mitochondrion membrane By similarity; Multi-pass membrane protein By similarity
Other locations
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Mitochondrion
- mitochondrial outer membrane Source: MGI
- mitochondrion Source: ParkinsonsUK-UCL
Other locations
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | Mitochondrial intermembrane1 Publication | 5 | |
Transmembranei | 6 – 26 | Helical; Name=1Add BLAST | 21 | |
Topological domaini | 27 – 46 | Cytoplasmic1 PublicationAdd BLAST | 20 | |
Transmembranei | 47 – 67 | Helical; Name=2Add BLAST | 21 | |
Topological domaini | 68 – 79 | Mitochondrial intermembrane1 PublicationAdd BLAST | 12 | |
Transmembranei | 80 – 100 | Helical; Name=3Add BLAST | 21 | |
Topological domaini | 101 – 105 | Cytoplasmic1 Publication | 5 | |
Transmembranei | 106 – 126 | Helical; Name=4Add BLAST | 21 | |
Topological domaini | 127 – 134 | Mitochondrial intermembrane1 Publication | 8 | |
Transmembranei | 135 – 155 | Helical; Name=5Add BLAST | 21 | |
Topological domaini | 156 – 169 | Cytoplasmic1 PublicationAdd BLAST | 14 |
Keywords - Cellular componenti
Membrane, MitochondrionPathology & Biotechi
Disruption phenotypei
No obvious phenotype. Mice are viable and fertile and present only very minor changes in gonadal and adrenal steroid hormone production. testis-specific gene disruption (PubMed:24174323) does not affect testosterone production, gametogenesis and male fertility.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 147 | A → T: No effect. 1 Publication | 1 | |
Mutagenesisi | 153 | Y → S: Abolishes cholesterol transport. 1 Publication | 1 | |
Mutagenesisi | 156 | R → L: Abolishes cholesterol transport. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2149 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190998 | 1 – 169 | Translocator proteinAdd BLAST | 169 |
Proteomic databases
jPOSTi | P50637 |
PaxDbi | P50637 |
PeptideAtlasi | P50637 |
PRIDEi | P50637 |
TopDownProteomicsi | P50637 |
PTM databases
iPTMneti | P50637 |
PhosphoSitePlusi | P50637 |
Expressioni
Tissue specificityi
Detected in liver (at protein level). Ubiquitous.2 Publications
Inductioni
By dimethyl sulfoxide and diazepam.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000041736, Expressed in adrenal gland and 275 other tissues |
ExpressionAtlasi | P50637, baseline and differential |
Genevisiblei | P50637, MM |
Interactioni
Subunit structurei
Interacts with TSPOAP1.
Interacts with MOST-1. May interact with STAR.
By similarityGO - Molecular functioni
- ion channel binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 198410, 2 interactors |
STRINGi | 10090.ENSMUSP00000037039 |
Chemistry databases
BindingDBi | P50637 |
Miscellaneous databases
RNActi | P50637, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P50637 |
SMRi | P50637 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the TspO/BZRP family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3797, Eukaryota |
GeneTreei | ENSGT00390000012980 |
HOGENOMi | CLU_091805_2_1_1 |
InParanoidi | P50637 |
OMAi | PPNWLFA |
OrthoDBi | 1592225at2759 |
PhylomeDBi | P50637 |
TreeFami | TF342852 |
Family and domain databases
CDDi | cd15904, TSPO_MBR, 1 hit |
Gene3Di | 1.20.1260.100, 1 hit |
InterProi | View protein in InterPro IPR030164, TSPO IPR038330, TspO/MBR-related_sf IPR004307, TspO_MBR |
PANTHERi | PTHR10057, PTHR10057, 1 hit PTHR10057:SF5, PTHR10057:SF5, 1 hit |
Pfami | View protein in Pfam PF03073, TspO_MBR, 1 hit |
PIRSFi | PIRSF005859, PBR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P50637-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPESWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA
60 70 80 90 100
PIWGTLYSAM GYGSYIVWKE LGGFTEDAMV PLGLYTGQLA LNWAWPPIFF
110 120 130 140 150
GARQMGWALA DLLLVSGVAT ATTLAWHRVS PPAARLLYPY LAWLAFATVL
160
NYYVWRDNSG RRGGSRLPE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140LIU9 | A0A140LIU9_MOUSE | Peripheral-type benzodiazepine rece... | Tspo | 102 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | S → G in AAH02055 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 54 | G → A in BAA04749 (PubMed:8125973).Curated | 1 | |
Sequence conflicti | 168 | P → A in BAA04749 (PubMed:8125973).Curated | 1 | |
Sequence conflicti | 168 | P → A in AAH02055 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L17306 mRNA Translation: AAA20127.1 D21207 mRNA Translation: BAA04749.1 AY079441 Genomic DNA Translation: AAL87529.1 AY079442 Genomic DNA Translation: AAL87530.1 AK149743 mRNA Translation: BAE29056.1 AK150516 mRNA Translation: BAE29628.1 AK151421 mRNA Translation: BAE30386.1 AK151569 mRNA Translation: BAE30511.1 AK151824 mRNA Translation: BAE30721.1 AK152082 mRNA Translation: BAE30932.1 AK152827 mRNA Translation: BAE31528.1 AK153159 mRNA Translation: BAE31769.1 AK153273 mRNA Translation: BAE31860.1 AK156489 mRNA Translation: BAE33730.1 AK165450 mRNA Translation: BAE38192.1 AK165822 mRNA Translation: BAE38396.1 AK166125 mRNA Translation: BAE38585.1 AK172384 mRNA Translation: BAE42976.1 CH466550 Genomic DNA Translation: EDL04468.1 BC002055 mRNA Translation: AAH02055.1 U12419 Genomic DNA Translation: AAA83253.1 |
CCDSi | CCDS27705.1 |
PIRi | A53405 I57953 |
RefSeqi | NP_033905.3, NM_009775.4 |
Genome annotation databases
Ensembli | ENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736 |
GeneIDi | 12257 |
KEGGi | mmu:12257 |
UCSCi | uc007xbg.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L17306 mRNA Translation: AAA20127.1 D21207 mRNA Translation: BAA04749.1 AY079441 Genomic DNA Translation: AAL87529.1 AY079442 Genomic DNA Translation: AAL87530.1 AK149743 mRNA Translation: BAE29056.1 AK150516 mRNA Translation: BAE29628.1 AK151421 mRNA Translation: BAE30386.1 AK151569 mRNA Translation: BAE30511.1 AK151824 mRNA Translation: BAE30721.1 AK152082 mRNA Translation: BAE30932.1 AK152827 mRNA Translation: BAE31528.1 AK153159 mRNA Translation: BAE31769.1 AK153273 mRNA Translation: BAE31860.1 AK156489 mRNA Translation: BAE33730.1 AK165450 mRNA Translation: BAE38192.1 AK165822 mRNA Translation: BAE38396.1 AK166125 mRNA Translation: BAE38585.1 AK172384 mRNA Translation: BAE42976.1 CH466550 Genomic DNA Translation: EDL04468.1 BC002055 mRNA Translation: AAH02055.1 U12419 Genomic DNA Translation: AAA83253.1 |
CCDSi | CCDS27705.1 |
PIRi | A53405 I57953 |
RefSeqi | NP_033905.3, NM_009775.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MGY | NMR | - | A | 1-167 | [»] | |
2N02 | NMR | - | A | 1-169 | [»] | |
BMRBi | P50637 | |||||
SMRi | P50637 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198410, 2 interactors |
STRINGi | 10090.ENSMUSP00000037039 |
Chemistry databases
BindingDBi | P50637 |
ChEMBLi | CHEMBL2149 |
PTM databases
iPTMneti | P50637 |
PhosphoSitePlusi | P50637 |
Proteomic databases
jPOSTi | P50637 |
PaxDbi | P50637 |
PeptideAtlasi | P50637 |
PRIDEi | P50637 |
TopDownProteomicsi | P50637 |
Protocols and materials databases
Antibodypediai | 27480, 268 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736 |
GeneIDi | 12257 |
KEGGi | mmu:12257 |
UCSCi | uc007xbg.2, mouse |
Organism-specific databases
CTDi | 706 |
MGIi | MGI:88222, Tspo |
Phylogenomic databases
eggNOGi | KOG3797, Eukaryota |
GeneTreei | ENSGT00390000012980 |
HOGENOMi | CLU_091805_2_1_1 |
InParanoidi | P50637 |
OMAi | PPNWLFA |
OrthoDBi | 1592225at2759 |
PhylomeDBi | P50637 |
TreeFami | TF342852 |
Enzyme and pathway databases
Reactomei | R-MMU-196108, Pregnenolone biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 12257, 3 hits in 18 CRISPR screens |
ChiTaRSi | Tspo, mouse |
PROi | PR:P50637 |
RNActi | P50637, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041736, Expressed in adrenal gland and 275 other tissues |
ExpressionAtlasi | P50637, baseline and differential |
Genevisiblei | P50637, MM |
Family and domain databases
CDDi | cd15904, TSPO_MBR, 1 hit |
Gene3Di | 1.20.1260.100, 1 hit |
InterProi | View protein in InterPro IPR030164, TSPO IPR038330, TspO/MBR-related_sf IPR004307, TspO_MBR |
PANTHERi | PTHR10057, PTHR10057, 1 hit PTHR10057:SF5, PTHR10057:SF5, 1 hit |
Pfami | View protein in Pfam PF03073, TspO_MBR, 1 hit |
PIRSFi | PIRSF005859, PBR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TSPO_MOUSE | |
Accessioni | P50637Primary (citable) accession number: P50637 Secondary accession number(s): Q541E3 Q99M32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families