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UniProtKB - P50621 (RIR2_BACSU)
Protein
Ribonucleoside-diphosphate reductase subunit beta
Gene
nrdF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
By similarityCatalytic activityi
- [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphatePROSITE-ProRule annotationEC:1.17.4.1PROSITE-ProRule annotation
Cofactori
Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity
: DNA replication Pathwayi
This protein is involved in the pathway DNA replication, which is part of Genetic information processing.View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 66 | Iron 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 97 | Iron 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 97 | Iron 2By similarity | 1 | |
Metal bindingi | 101 | Iron 1PROSITE-ProRule annotation | 1 | |
Active sitei | 105 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 164 | Iron 2By similarity | 1 | |
Metal bindingi | 198 | Iron 2By similarity | 1 | |
Metal bindingi | 201 | Iron 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
GO - Biological processi
- deoxyribonucleotide biosynthetic process Source: InterPro
- DNA replication Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | DNA replication |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU17390-MONOMER |
UniPathwayi | UPA00326 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)Alternative name(s): Ribonucleotide reductase small subunit |
Gene namesi | Name:nrdF Ordered Locus Names:BSU17390 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Essential, at least the last 3 genes of the locus cannot be deleted; could be due to polar effects on downstream ymaB.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190469 | 1 – 329 | Ribonucleoside-diphosphate reductase subunit betaAdd BLAST | 329 |
Proteomic databases
jPOSTi | P50621 |
PaxDbi | P50621 |
Expressioni
Inductioni
Part of the probable nrdI(ymaA)-nrdE-nrdF-ymaB operon. Expression is constitutive but low, dramatically induced by thymidine starvation which requires recA.1 Publication
Interactioni
Subunit structurei
Tetramer of two alpha and two beta subunits.
By similarityProtein-protein interaction databases
STRINGi | 224308.BSU17390 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P50621 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the ribonucleoside diphosphate reductase small chain family.Curated
Phylogenomic databases
eggNOGi | COG0208, Bacteria |
InParanoidi | P50621 |
OMAi | DFFFYTN |
PhylomeDBi | P50621 |
Family and domain databases
CDDi | cd01049, RNRR2, 1 hit |
Gene3Di | 1.10.620.20, 1 hit |
InterProi | View protein in InterPro IPR009078, Ferritin-like_SF IPR012348, RNR-like IPR026494, RNR_NrdF-like IPR033909, RNR_small IPR030475, RNR_small_AS IPR000358, RNR_small_fam |
PANTHERi | PTHR23409, PTHR23409, 1 hit |
Pfami | View protein in Pfam PF00268, Ribonuc_red_sm, 1 hit |
PIRSFi | PIRSF000355, NrdB, 1 hit |
SUPFAMi | SSF47240, SSF47240, 1 hit |
TIGRFAMsi | TIGR04171, RNR_1b_NrdF, 1 hit |
PROSITEi | View protein in PROSITE PS00368, RIBORED_SMALL, 1 hit |
i Sequence
Sequence statusi: Complete.
P50621-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKIYDAANW SKHEDDFTQM FYNQNVKQFW LPEEIALNGD LLTWKYLGKN
60 70 80 90 100
EQDTYMKVLA GLTLLDTEQG NTGMPIVAEH VDGHQRKAVL NFMAMMENAV
110 120 130 140 150
HAKSYSNIFM TLAPTETINE VFEWVKQNKY LQKKAQMIVG LYKAIQKDDE
160 170 180 190 200
ISLFKAMVAS VYLESFLFYS GFYYPLYFYG QGKLMQSGEI INLILRDEAI
210 220 230 240 250
HGVYVGLLAQ EIYNKQTEEK KAELREFAID LLNQLYENEL EYTEDLYDQV
260 270 280 290 300
GLSHDVKKFI RYNANKALMN LGFDPYFEEE DINPIVLNGL NTKTKSHDFF
310 320
SMKGNGYKKA TVEPLKDDDF YFEDEKEQI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z68500 Genomic DNA Translation: CAA92811.1 AL009126 Genomic DNA Translation: CAB13623.1 |
PIRi | C69667 |
RefSeqi | NP_389621.1, NC_000964.3 WP_003231754.1, NZ_CP053102.1 |
Genome annotation databases
EnsemblBacteriai | CAB13623; CAB13623; BSU_17390 |
GeneIDi | 940076 |
KEGGi | bsu:BSU17390 |
PATRICi | fig|224308.179.peg.1885 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z68500 Genomic DNA Translation: CAA92811.1 AL009126 Genomic DNA Translation: CAB13623.1 |
PIRi | C69667 |
RefSeqi | NP_389621.1, NC_000964.3 WP_003231754.1, NZ_CP053102.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4DR0 | X-ray | 1.90 | A/B | 1-329 | [»] | |
SMRi | P50621 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU17390 |
Proteomic databases
jPOSTi | P50621 |
PaxDbi | P50621 |
Genome annotation databases
EnsemblBacteriai | CAB13623; CAB13623; BSU_17390 |
GeneIDi | 940076 |
KEGGi | bsu:BSU17390 |
PATRICi | fig|224308.179.peg.1885 |
Phylogenomic databases
eggNOGi | COG0208, Bacteria |
InParanoidi | P50621 |
OMAi | DFFFYTN |
PhylomeDBi | P50621 |
Enzyme and pathway databases
UniPathwayi | UPA00326 |
BioCyci | BSUB:BSU17390-MONOMER |
Family and domain databases
CDDi | cd01049, RNRR2, 1 hit |
Gene3Di | 1.10.620.20, 1 hit |
InterProi | View protein in InterPro IPR009078, Ferritin-like_SF IPR012348, RNR-like IPR026494, RNR_NrdF-like IPR033909, RNR_small IPR030475, RNR_small_AS IPR000358, RNR_small_fam |
PANTHERi | PTHR23409, PTHR23409, 1 hit |
Pfami | View protein in Pfam PF00268, Ribonuc_red_sm, 1 hit |
PIRSFi | PIRSF000355, NrdB, 1 hit |
SUPFAMi | SSF47240, SSF47240, 1 hit |
TIGRFAMsi | TIGR04171, RNR_1b_NrdF, 1 hit |
PROSITEi | View protein in PROSITE PS00368, RIBORED_SMALL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIR2_BACSU | |
Accessioni | P50621Primary (citable) accession number: P50621 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families