UniProtKB - P50586 (TUB_MOUSE)
Protein
Tubby protein
Gene
Tub
Organism
Mus musculus (Mouse)
Status
Functioni
Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight. Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages (By similarity).By similarity2 Publications
GO - Molecular functioni
- G protein-coupled receptor binding Source: MGI
- intraciliary transport particle A binding Source: MGI
- protein-containing complex binding Source: MGI
GO - Biological processi
- intraciliary transport Source: MGI
- phagocytosis, recognition Source: MGI
- photoreceptor cell maintenance Source: MGI
- positive regulation of phagocytosis Source: UniProtKB
- protein localization to ciliary membrane Source: MGI
- protein localization to cilium Source: MGI
- protein localization to photoreceptor outer segment Source: MGI
- receptor localization to non-motile cilium Source: MGI
- regulation of G protein-coupled receptor signaling pathway Source: MGI
- response to hormone Source: Ensembl
- retina development in camera-type eye Source: MGI
- sensory perception of sound Source: MGI
Keywordsi
Biological process | Phagocytosis, Sensory transduction |
Names & Taxonomyi
Protein namesi | Recommended name: Tubby protein |
Gene namesi | Name:Tub Synonyms:Rd5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2651573, Tub |
Subcellular locationi
Extracellular region or secreted
Nucleus
Plasma membrane
Other locations
Note: Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular region Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, Nucleus, SecretedPathology & Biotechi
Involvement in diseasei
Defects in Tub are the cause of maturity-onset obesity, insulin resistance and sensory deficits.
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 330 | K → A: Abolishes interaction with phosphatidylinositol 4,5-bisphosphate and reduces secretion by 40%; when associated with A-332. 2 Publications | 1 | |
Mutagenesisi | 332 | R → A: Abolishes interaction with phosphatidylinositol 4,5-bisphosphate and reduces secretion by 40%; when associated with A-330. 2 Publications | 1 |
Keywords - Diseasei
ObesityPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186464 | 1 – 505 | Tubby proteinAdd BLAST | 505 |
Proteomic databases
PaxDbi | P50586 |
PeptideAtlasi | P50586 |
PRIDEi | P50586 |
PTM databases
iPTMneti | P50586 |
PhosphoSitePlusi | P50586 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031028, Expressed in retinal neural layer and 262 other tissues |
Genevisiblei | P50586, MM |
Interactioni
Subunit structurei
Interacts with GNAQ.
Interacts with TULP1.
2 PublicationsGO - Molecular functioni
- G protein-coupled receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204371, 6 interactors |
STRINGi | 10090.ENSMUSP00000033341 |
Miscellaneous databases
RNActi | P50586, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P50586 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P50586 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 197 – 204 | Asp/Glu-rich | 8 | |
Compositional biasi | 206 – 210 | Poly-Ser | 5 |
Sequence similaritiesi
Belongs to the TUB family.Curated
Phylogenomic databases
eggNOGi | KOG2502, Eukaryota |
GeneTreei | ENSGT00940000158372 |
InParanoidi | P50586 |
OMAi | SMSFDEE |
PhylomeDBi | P50586 |
TreeFami | TF314076 |
Family and domain databases
Gene3Di | 3.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR025659, Tubby-like_C IPR000007, Tubby_C IPR018066, Tubby_C_CS IPR005398, Tubby_N |
Pfami | View protein in Pfam PF01167, Tub, 1 hit PF16322, Tub_N, 1 hit |
PRINTSi | PR01573, SUPERTUBBY PR01574, TUBBYPROTEIN |
SUPFAMi | SSF54518, SSF54518, 1 hit |
PROSITEi | View protein in PROSITE PS01200, TUB_1, 1 hit PS01201, TUB_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform Long (identifier: P50586-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSKPHSDWI PYSVLDDEGS NLRQQKLDRQ RALLEQKQKK KRQEPLMVQA
60 70 80 90 100
NADGRPRSRR ARQSEEQAPL VESYLSSSGS TSYQVQEADS IASVQLGATR
110 120 130 140 150
PPAPASAKKS KGAAASGGQG GAPRKEKKGK HKGTSGPATL AEDKSEAQGP
160 170 180 190 200
VQILTVGQSD HDKDAGETAA GGGAQPSGQD LRATMQRKGI SSSMSFDEDE
210 220 230 240 250
DEDENSSSSS QLNSNTRPSS ATSRKSIREA ASAPSPAAPE PPVDIEVQDL
260 270 280 290 300
EEFALRPAPQ GITIKCRITR DKKGMDRGMY PTYFLHLDRE DGKKVFLLAG
310 320 330 340 350
RKRKKSKTSN YLISVDPTDL SRGGDSYIGK LRSNLMGTKF TVYDNGVNPQ
360 370 380 390 400
KASSSTLESG TLRQELAAVC YETNVLGFKG PRKMSVIVPG MNMVHERVCI
410 420 430 440 450
RPRNEHETLL ARWQNKNTES IIELQNKTPV WNDDTQSYVL NFHGRVTQAS
460 470 480 490 500
VKNFQIIHGN DPDYIVMQFG RVAEDVFTMD YNYPLCALQA FAIALSSFDS
KLACE
Isoform Short (identifier: P50586-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
153-208: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1P1 | A0A0R4J1P1_MOUSE | Tubby-like protein | Tub | 459 | Annotation score: | ||
Q3UEX1 | Q3UEX1_MOUSE | Tubby protein | Tub | 167 | Annotation score: |
Sequence cautioni
The sequence AAC52512 differs from that shown. Reason: Erroneous initiation.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006675 | 153 – 208 | Missing in isoform Short. CuratedAdd BLAST | 56 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54643 mRNA Translation: AAB53495.1 U52433 mRNA Translation: AAC52510.1 U52824 mRNA Translation: AAC52512.1 Different initiation. AJ296303 Genomic DNA Translation: CAC39309.1 |
CCDSi | CCDS21732.1 [P50586-1] |
PIRi | S68518 |
RefSeqi | NP_068685.1, NM_021885.4 [P50586-1] |
Genome annotation databases
Ensembli | ENSMUST00000033341; ENSMUSP00000033341; ENSMUSG00000031028 [P50586-1] |
GeneIDi | 22141 |
KEGGi | mmu:22141 |
UCSCi | uc009jdf.1, mouse [P50586-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54643 mRNA Translation: AAB53495.1 U52433 mRNA Translation: AAC52510.1 U52824 mRNA Translation: AAC52512.1 Different initiation. AJ296303 Genomic DNA Translation: CAC39309.1 |
CCDSi | CCDS21732.1 [P50586-1] |
PIRi | S68518 |
RefSeqi | NP_068685.1, NM_021885.4 [P50586-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C8Z | X-ray | 1.90 | A | 243-505 | [»] | |
1I7E | X-ray | 1.95 | A | 243-505 | [»] | |
SMRi | P50586 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204371, 6 interactors |
STRINGi | 10090.ENSMUSP00000033341 |
PTM databases
iPTMneti | P50586 |
PhosphoSitePlusi | P50586 |
Proteomic databases
PaxDbi | P50586 |
PeptideAtlasi | P50586 |
PRIDEi | P50586 |
Protocols and materials databases
Antibodypediai | 11439, 86 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033341; ENSMUSP00000033341; ENSMUSG00000031028 [P50586-1] |
GeneIDi | 22141 |
KEGGi | mmu:22141 |
UCSCi | uc009jdf.1, mouse [P50586-1] |
Organism-specific databases
CTDi | 7275 |
MGIi | MGI:2651573, Tub |
Phylogenomic databases
eggNOGi | KOG2502, Eukaryota |
GeneTreei | ENSGT00940000158372 |
InParanoidi | P50586 |
OMAi | SMSFDEE |
PhylomeDBi | P50586 |
TreeFami | TF314076 |
Miscellaneous databases
BioGRID-ORCSi | 22141, 0 hits in 17 CRISPR screens |
EvolutionaryTracei | P50586 |
PROi | PR:P50586 |
RNActi | P50586, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031028, Expressed in retinal neural layer and 262 other tissues |
Genevisiblei | P50586, MM |
Family and domain databases
Gene3Di | 3.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR025659, Tubby-like_C IPR000007, Tubby_C IPR018066, Tubby_C_CS IPR005398, Tubby_N |
Pfami | View protein in Pfam PF01167, Tub, 1 hit PF16322, Tub_N, 1 hit |
PRINTSi | PR01573, SUPERTUBBY PR01574, TUBBYPROTEIN |
SUPFAMi | SSF54518, SSF54518, 1 hit |
PROSITEi | View protein in PROSITE PS01200, TUB_1, 1 hit PS01201, TUB_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TUB_MOUSE | |
Accessioni | P50586Primary (citable) accession number: P50586 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families