Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proliferation-associated protein 2G4

Gene

Pa2g4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly (By similarity). Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Together with PTBP1 is required for the translation initiation on the foot-and-mouth disease virus (FMDV) IRES. Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).By similarity2 Publications

Caution

Although it belongs to the peptidase M24 family, it does not contain metal cofactors and lacks aminopeptidase activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, Ribonucleoprotein, RNA-binding
Biological processrRNA processing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiM24.973

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferation-associated protein 2G4
Alternative name(s):
IRES-specific cellular trans-acting factor 45 kDa
Short name:
ITAF45
Mpp1
Proliferation-associated protein 1
Protein p38-2G4
Gene namesi
Name:Pa2g4
Synonyms:Ebp1, Plfap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:894684 Pa2g4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65 – 72KKEKEMKK → SSSSSSSS: No effect on RNA-binding. 1 Publication8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001489902 – 394Proliferation-associated protein 2G4Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Cross-linki298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei335PhosphoserineBy similarity1
Modified residuei361Phosphoserine; by PKC/PRKCDBy similarity1
Modified residuei366PhosphothreonineBy similarity1
Modified residuei386PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.By similarity
Isoform 2 is polyubiquitinated, leading to proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP50580
PaxDbiP50580
PeptideAtlasiP50580
PRIDEiP50580

PTM databases

iPTMnetiP50580
PhosphoSitePlusiP50580
SwissPalmiP50580

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed in proliferating cells. Observed between G1 and mid S phase, decrease toward the end of S phase, and disappear at the S/G2 transition.1 Publication

Inductioni

By mitogens.1 Publication

Gene expression databases

BgeeiENSMUSG00000025364
CleanExiMM_PA2G4
ExpressionAtlasiP50580 baseline and differential
GenevisibleiP50580 MM

Interactioni

Subunit structurei

Isoform 2 interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with nucleolin/NCL. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation (By similarity). Interacts with AKT1 (By similarity). Isoform 1 and isoform 2 interact with RNF20 (By similarity). Isoform 2 interacts with HUWE1. Interacts with DNAJC21 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202246, 6 interactors
IntActiP50580, 7 interactors
MINTiP50580
STRINGi10090.ENSMUSP00000026425

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 36Combined sources22
Helixi45 – 60Combined sources16
Beta strandi72 – 82Combined sources11
Beta strandi85 – 87Combined sources3
Beta strandi93 – 95Combined sources3
Beta strandi105 – 114Combined sources10
Beta strandi117 – 126Combined sources10
Helixi138 – 156Combined sources19
Helixi163 – 176Combined sources14
Beta strandi186 – 189Combined sources4
Beta strandi194 – 196Combined sources3
Beta strandi198 – 204Combined sources7
Helixi207 – 212Combined sources6
Beta strandi223 – 233Combined sources11
Beta strandi246 – 249Combined sources4
Helixi260 – 272Combined sources13
Turni273 – 275Combined sources3
Helixi280 – 282Combined sources3
Helixi286 – 298Combined sources13
Beta strandi301 – 305Combined sources5
Beta strandi316 – 326Combined sources11
Beta strandi329 – 332Combined sources4
Helixi340 – 342Combined sources3
Helixi352 – 357Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V6CX-ray2.50A9-360[»]
ProteinModelPortaliP50580
SMRiP50580
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50580

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 48Necessary for nucleolar localizationBy similarityAdd BLAST47
Regioni46 – 54RNA-bindingBy similarity9
Regioni301 – 394Necessary for nucleolar localizationBy similarityAdd BLAST94
Regioni361 – 375Interaction with RNA1 PublicationAdd BLAST15

Sequence similaritiesi

Belongs to the peptidase M24 family.Curated

Phylogenomic databases

eggNOGiKOG2776 Eukaryota
COG0024 LUCA
GeneTreeiENSGT00530000063220
HOGENOMiHOG000168207
HOVERGENiHBG053117
InParanoidiP50580
OMAiHTVLLMP
OrthoDBiEOG091G0B89
PhylomeDBiP50580
TreeFamiTF300010

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR004545 PA2G4
IPR000994 Pept_M24
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 2 hits
TIGRFAMsiTIGR00495 crvDNA_42K, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: P50580-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE
60 70 80 90 100
KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL
110 120 130 140 150
KEGDLVKIDL GVHVDGFIAN VAHTFVIGVA QGTQVTGRKA DVIKAAHLCA
160 170 180 190 200
EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT
210 220 230 240 250
IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD
260 270 280 290 300
PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE
310 320 330 340 350
LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ
360 370 380 390
DAELKALLQS SASRKTQKKK KKKASKTVEN ATSGETLEEN GAGD
Length:394
Mass (Da):43,699
Last modified:January 23, 2007 - v3
Checksum:iABCD169F064261EB
GO
Isoform 2Curated (identifier: P50580-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Show »
Length:340
Mass (Da):37,971
Checksum:i0512D357D48D910F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279T → A no nucleotide entry (PubMed:10950867).Curated1
Sequence conflicti311K → R no nucleotide entry (PubMed:10950867).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0573261 – 54Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84789 mRNA Translation: CAA59260.1
U43918 mRNA Translation: AAB60513.1
BC046532 mRNA Translation: AAH46532.1
CCDSiCCDS24282.1 [P50580-1]
PIRiI48702 S54181
RefSeqiNP_035249.1, NM_011119.3 [P50580-1]
UniGeneiMm.4742

Genome annotation databases

EnsembliENSMUST00000026425; ENSMUSP00000026425; ENSMUSG00000025364 [P50580-1]
ENSMUST00000131728; ENSMUSP00000114434; ENSMUSG00000025364 [P50580-1]
GeneIDi18813
KEGGimmu:18813
UCSCiuc007hnl.1 mouse [P50580-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPA2G4_MOUSE
AccessioniPrimary (citable) accession number: P50580
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health