UniProtKB - P50552 (VASP_HUMAN)
Vasodilator-stimulated phosphoprotein
VASP
Functioni
Miscellaneous
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- cadherin binding Source: BHF-UCL
- profilin binding Source: UniProtKB
- SH3 domain binding Source: GO_Central
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- actin polymerization or depolymerization Source: InterPro
- axon guidance Source: GO_Central
- cell junction assembly Source: Reactome
- neural tube closure Source: GO_Central
- positive regulation of actin filament polymerization Source: UniProtKB
- protein homotetramerization Source: InterPro
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
PathwayCommonsi | P50552 |
Reactomei | R-HSA-202433, Generation of second messenger molecules R-HSA-376176, Signaling by ROBO receptors R-HSA-446353, Cell-extracellular matrix interactions |
SIGNORi | P50552 |
Protein family/group databases
MoonDBi | P50552, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Vasodilator-stimulated phosphoproteinShort name: VASP |
Gene namesi | Name:VASP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000125753.13 |
HGNCi | HGNC:12652, VASP |
MIMi | 601703, gene |
neXtProti | NX_P50552 |
Subcellular locationi
Plasma membrane
Cytoskeleton
Other locations
- Cytoplasm
- focal adhesion
- tight junction By similarity
Note: Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).
Cytoskeleton
- actin cytoskeleton Source: ProtInc
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- filopodium membrane Source: UniProtKB-SubCell
- lamellipodium membrane Source: UniProtKB-SubCell
- plasma membrane Source: HPA
Other locations
- bicellular tight junction Source: UniProtKB-SubCell
- focal adhesion Source: HPA
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 157 | S → A: Promotes F-actin assembly; when associated with A-239 and A-278. Interferes with F-actin assembly; when associated with A-239 and E-278. 1 Publication | 1 | |
Mutagenesisi | 239 | S → A: Promotes F-actin assembly; when associated with A-157 and A-278. Interferes with F-actin assembly; when associated with A-157 and E-278. 1 Publication | 1 | |
Mutagenesisi | 278 | T → A: Promotes F-actin assembly; when associated with A-157 and A-239. 1 Publication | 1 | |
Mutagenesisi | 278 | T → E: Interferes with F-actin assembly; when associated with A-157 and A-239. 1 Publication | 1 | |
Mutagenesisi | 370 | F → A: Lower stability of tetramerization domain. 1 Publication | 1 | |
Mutagenesisi | 370 | F → I or K: No change in stability of tetramerization domain. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 7408 |
OpenTargetsi | ENSG00000125753 |
PharmGKBi | PA37276 |
Miscellaneous databases
Pharosi | P50552, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295774 |
Polymorphism and mutation databases
BioMutai | VASP |
DMDMi | 1718079 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000065767 | 2 – 380 | Vasodilator-stimulated phosphoproteinAdd BLAST | 379 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 39 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 46 | PhosphoserineCombined sources | 1 | |
Modified residuei | 157 | Phosphoserine; by PKA, PKC, PKG/PRKG1 and ROCK14 Publications | 1 | |
Modified residuei | 239 | Phosphoserine; by PKA and PKG/PRKG1Combined sources2 Publications | 1 | |
Modified residuei | 278 | Phosphothreonine; by PKA, PKG/PRKG1 and AMPK2 Publications | 1 | |
Modified residuei | 283 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 284 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 305 | PhosphoserineCombined sources | 1 | |
Modified residuei | 314 | PhosphoserineCombined sources | 1 | |
Modified residuei | 316 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 322 | Phosphoserine; by AMPKCombined sources | 1 | |
Modified residuei | 323 | PhosphoserineBy similarity | 1 | |
Modified residuei | 325 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P50552 |
jPOSTi | P50552 |
MassIVEi | P50552 |
MaxQBi | P50552 |
PaxDbi | P50552 |
PeptideAtlasi | P50552 |
PRIDEi | P50552 |
ProteomicsDBi | 56248 |
TopDownProteomicsi | P50552 |
2D gel databases
OGPi | P50552 |
PTM databases
iPTMneti | P50552 |
MetOSitei | P50552 |
PhosphoSitePlusi | P50552 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000125753, Expressed in blood and 220 other tissues |
ExpressionAtlasi | P50552, baseline and differential |
Genevisiblei | P50552, HS |
Organism-specific databases
HPAi | ENSG00000125753, Low tissue specificity |
Interactioni
Subunit structurei
Homotetramer.
Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1.
Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells.
Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA.
Interacts with APBB1IP.
Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP (By similarity).
Interacts weakly with MEFV.
By similarity9 PublicationsBinary interactionsi
Hide detailsP50552
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- cadherin binding Source: BHF-UCL
- profilin binding Source: UniProtKB
- SH3 domain binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 113251, 107 interactors |
CORUMi | P50552 |
DIPi | DIP-44105N |
ELMi | P50552 |
IntActi | P50552, 53 interactors |
MINTi | P50552 |
STRINGi | 9606.ENSP00000245932 |
Miscellaneous databases
RNActi | P50552, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P50552 |
SMRi | P50552 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P50552 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 113 | WH1PROSITE-ProRule annotationAdd BLAST | 112 | |
Repeati | 344 – 358 | 1Add BLAST | 15 | |
Repeati | 359 – 373 | 2Add BLAST | 15 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 225 – 377 | EVH2Add BLAST | 153 | |
Regioni | 225 – 245 | EVH2 block AAdd BLAST | 21 | |
Regioni | 259 – 278 | EVH2 block BAdd BLAST | 20 | |
Regioni | 343 – 377 | EVH2 block CAdd BLAST | 35 | |
Regioni | 344 – 373 | 2 X 15 AA tandem repeats of L-[EQ]-[KR]-[MV]-K-[EQ]-E-[IL]-[IL]-E-[AEV]-[FV]-[KRV]-[KQ]-EAdd BLAST | 30 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 337 – 373 | Sequence analysisAdd BLAST | 37 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 234 – 237 | KLKR | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 118 – 216 | Pro-richAdd BLAST | 99 | |
Compositional biasi | 215 – 222 | Poly-Gly | 8 | |
Compositional biasi | 259 – 262 | Poly-Gly | 4 | |
Compositional biasi | 322 – 325 | Poly-Ser | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Repeat, SH3-bindingPhylogenomic databases
eggNOGi | KOG4590, Eukaryota |
GeneTreei | ENSGT00940000156765 |
HOGENOMi | CLU_017790_0_0_1 |
InParanoidi | P50552 |
OMAi | IRGVKYN |
OrthoDBi | 263288at2759 |
PhylomeDBi | P50552 |
TreeFami | TF321411 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR042180, IF_rod_dom_coil1B IPR011993, PH-like_dom_sf IPR034367, VASP IPR017354, VASP/EVL IPR038023, VASP_sf IPR014885, VASP_tetra IPR000697, WH1/EVH1_dom |
PANTHERi | PTHR11202:SF12, PTHR11202:SF12, 1 hit |
Pfami | View protein in Pfam PF08776, VASP_tetra, 1 hit PF00568, WH1, 1 hit |
PIRSFi | PIRSF038010, Vasodilator_Phospo, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
SUPFAMi | SSF118370, SSF118370, 1 hit |
PROSITEi | View protein in PROSITE PS50229, WH1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV
60 70 80 90 100
GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA
110 120 130 140 150
QFAAGMASAL EALEGGGPPP PPALPTWSVP NGPSPEEVEQ QKRQQPGPSE
160 170 180 190 200
HIERRVSNAG GPPAPPAGGP PPPPGPPPPP GPPPPPGLPP SGVPAAAHGA
210 220 230 240 250
GGGPPPAPPL PAAQGPGGGG AGAPGLAAAI AGAKLRKVSK QEEASGGPTA
260 270 280 290 300
PKAESGRSGG GGLMEEMNAM LARRRKATQV GEKTPKDESA NQEEPEARVP
310 320 330 340 350
AQSESVRRPW EKNSTTLPRM KSSSSVTTSE TQPCTPSSSD YSDLQRVKQE
360 370 380
LLEEVKKELQ KVKEEIIEAF VQELRKRGSP
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EM16 | K7EM16_HUMAN | Vasodilator-stimulated phosphoprote... | VASP | 183 | Annotation score: | ||
K7ENL7 | K7ENL7_HUMAN | Vasodilator-stimulated phosphoprote... | VASP | 103 | Annotation score: | ||
K7EIG8 | K7EIG8_HUMAN | Vasodilator-stimulated phosphoprote... | VASP | 46 | Annotation score: | ||
K7ENR7 | K7ENR7_HUMAN | Vasodilator-stimulated phosphoprote... | VASP | 59 | Annotation score: | ||
K7EQD0 | K7EQD0_HUMAN | Vasodilator-stimulated phosphoprote... | VASP | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | S → SS in AAH26019 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 241 | Missing in AAH26019 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048929 | 104 | A → T. Corresponds to variant dbSNP:rs10415373EnsemblClinVar. | 1 | |
Natural variantiVAR_048930 | 140 | Q → H. Corresponds to variant dbSNP:rs34345197Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46389 mRNA Translation: CAA86523.1 CH471126 Genomic DNA Translation: EAW57362.1 AK314812 mRNA Translation: BAG37336.1 BC026019 mRNA Translation: AAH26019.1 BC038224 mRNA Translation: AAH38224.1 X98534, X98533 Genomic DNA Translation: CAA67147.2 |
CCDSi | CCDS33051.1 |
PIRi | S51797 |
RefSeqi | NP_003361.1, NM_003370.3 |
Genome annotation databases
Ensembli | ENST00000245932; ENSP00000245932; ENSG00000125753 |
GeneIDi | 7408 |
KEGGi | hsa:7408 |
UCSCi | uc002pcg.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46389 mRNA Translation: CAA86523.1 CH471126 Genomic DNA Translation: EAW57362.1 AK314812 mRNA Translation: BAG37336.1 BC026019 mRNA Translation: AAH26019.1 BC038224 mRNA Translation: AAH38224.1 X98534, X98533 Genomic DNA Translation: CAA67147.2 |
CCDSi | CCDS33051.1 |
PIRi | S51797 |
RefSeqi | NP_003361.1, NM_003370.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EGX | NMR | - | A | 1-115 | [»] | |
1JNG | model | - | A | 1-115 | [»] | |
1USD | X-ray | 1.70 | A | 336-380 | [»] | |
1USE | X-ray | 1.30 | A | 336-380 | [»] | |
2PAV | X-ray | 1.80 | V | 199-214 | [»] | |
2PBD | X-ray | 1.50 | V | 203-245 | [»] | |
3CHW | X-ray | 2.30 | V | 199-214 | [»] | |
BMRBi | P50552 | |||||
SMRi | P50552 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113251, 107 interactors |
CORUMi | P50552 |
DIPi | DIP-44105N |
ELMi | P50552 |
IntActi | P50552, 53 interactors |
MINTi | P50552 |
STRINGi | 9606.ENSP00000245932 |
Chemistry databases
ChEMBLi | CHEMBL4295774 |
Protein family/group databases
MoonDBi | P50552, Predicted |
PTM databases
iPTMneti | P50552 |
MetOSitei | P50552 |
PhosphoSitePlusi | P50552 |
Polymorphism and mutation databases
BioMutai | VASP |
DMDMi | 1718079 |
2D gel databases
OGPi | P50552 |
Proteomic databases
EPDi | P50552 |
jPOSTi | P50552 |
MassIVEi | P50552 |
MaxQBi | P50552 |
PaxDbi | P50552 |
PeptideAtlasi | P50552 |
PRIDEi | P50552 |
ProteomicsDBi | 56248 |
TopDownProteomicsi | P50552 |
Protocols and materials databases
Antibodypediai | 1491, 1061 antibodies |
Genome annotation databases
Ensembli | ENST00000245932; ENSP00000245932; ENSG00000125753 |
GeneIDi | 7408 |
KEGGi | hsa:7408 |
UCSCi | uc002pcg.4, human |
Organism-specific databases
CTDi | 7408 |
DisGeNETi | 7408 |
EuPathDBi | HostDB:ENSG00000125753.13 |
GeneCardsi | VASP |
HGNCi | HGNC:12652, VASP |
HPAi | ENSG00000125753, Low tissue specificity |
MIMi | 601703, gene |
neXtProti | NX_P50552 |
OpenTargetsi | ENSG00000125753 |
PharmGKBi | PA37276 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4590, Eukaryota |
GeneTreei | ENSGT00940000156765 |
HOGENOMi | CLU_017790_0_0_1 |
InParanoidi | P50552 |
OMAi | IRGVKYN |
OrthoDBi | 263288at2759 |
PhylomeDBi | P50552 |
TreeFami | TF321411 |
Enzyme and pathway databases
PathwayCommonsi | P50552 |
Reactomei | R-HSA-202433, Generation of second messenger molecules R-HSA-376176, Signaling by ROBO receptors R-HSA-446353, Cell-extracellular matrix interactions |
SIGNORi | P50552 |
Miscellaneous databases
BioGRID-ORCSi | 7408, 9 hits in 839 CRISPR screens |
ChiTaRSi | VASP, human |
EvolutionaryTracei | P50552 |
GeneWikii | Vasodilator-stimulated_phosphoprotein |
GenomeRNAii | 7408 |
Pharosi | P50552, Tbio |
PROi | PR:P50552 |
RNActi | P50552, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000125753, Expressed in blood and 220 other tissues |
ExpressionAtlasi | P50552, baseline and differential |
Genevisiblei | P50552, HS |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR042180, IF_rod_dom_coil1B IPR011993, PH-like_dom_sf IPR034367, VASP IPR017354, VASP/EVL IPR038023, VASP_sf IPR014885, VASP_tetra IPR000697, WH1/EVH1_dom |
PANTHERi | PTHR11202:SF12, PTHR11202:SF12, 1 hit |
Pfami | View protein in Pfam PF08776, VASP_tetra, 1 hit PF00568, WH1, 1 hit |
PIRSFi | PIRSF038010, Vasodilator_Phospo, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
SUPFAMi | SSF118370, SSF118370, 1 hit |
PROSITEi | View protein in PROSITE PS50229, WH1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VASP_HUMAN | |
Accessioni | P50552Primary (citable) accession number: P50552 Secondary accession number(s): B2RBT9, Q6PIZ1, Q93035 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 201 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations