UniProtKB - P50540 (MXI1_MOUSE)

BLAST

>sp|P50540|MXI1_MOUSE Max-interacting protein 1 OS=Mus musculus OX=10090 GN=Mxi1 PE=1 SV=1
MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPRLQHSKPPRRLSRAQKHSSG
SSNTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGPDCTRHTTLGLLNKAKAHIKKLE
EAERKSQHQLENLEREQRFLKRRLEQLQGPQEMERIRMDSIGSTISSDRSDSEREEIEVD
VESTEFSHGEADSVSTTSISDLDDHSSLQSVGSDEGYSSASVKLSFAS

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History
Entry version 138 (07 Nov 2018)
Sequence version 1 (01 Oct 1996)
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Protein

Max-interacting protein 1

Gene

Mxi1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Isoform Short, which lacks a segment, has a much stronger suppressive potential and associates with a SIN3 homologous protein.

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW
protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Max-interacting protein 1 Short name: Max interactor 1
Gene namesⁱ	Name:Mxi1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:97245 Mxi1

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127286	1 – 228	Max-interacting protein 1Add BLAST		228

Proteomic databases

PaxDbⁱ	P50540
PRIDEⁱ	P50540

PTM databases

PhosphoSitePlusⁱ	P50540

PhosphoSitePlusⁱ

P50540

Expressionⁱ

Developmental stageⁱ

Primarily expressed in differentiating cells. Also expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic day 10.5, expressed in the ventricular zone of the neural tube. Expressed at highest levels in cells accumulating in the intermediate zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17 dpc, expression is restricted not only to the differentiating cells of the epidermis but also to the proliferating cell compartment and expression extends into the first differentiating cell layers, but decreases in the uppermost layers of the epidermis.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000025025 Expressed in 303 organ(s), highest expression level in blood
CleanExⁱ	MM_MXI1
ExpressionAtlasⁱ	P50540 baseline and differential
Genevisibleⁱ	P50540 MM

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SMC3. Interacts with RNF17.2 Publications

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201630, 5 interactors
Database of interacting proteins More...DIPⁱ	DIP-489N
IntActⁱ	P50540, 1 interactor
STRINGⁱ	10090.ENSMUSP00000003870

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P50540
SMRⁱ	P50540
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	67 – 119	bHLHPROSITE-ProRule annotationAdd BLAST		53

Phylogenomic databases

eggNOGⁱ	ENOG410IPD5 Eukaryota ENOG4111MIN LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046360
HOGENOMⁱ	HOG000247060
HOVERGENⁱ	HBG006314
InParanoidⁱ	P50540
KEGG Orthology (KO) More...KOⁱ	K09114
OMAⁱ	GLQDPKP
PhylomeDBⁱ	P50540

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform Long (identifier: P50540-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERVRMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR 
        60         70         80         90        100
LSRAQKHSSG SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC 
       110        120        130        140        150
TRHTTLGLLN KAKAHIKKLE EAERKSQHQL ENLEREQRFL KRRLEQLQGP 
       160        170        180        190        200
QEMERIRMDS IGSTISSDRS DSEREEIEVD VESTEFSHGE ADSVSTTSIS 
       210        220 
DLDDHSSLQS VGSDEGYSSA SVKLSFAS

Length:228

Mass (Da):25,978

Last modified:October 1, 1996 - v1

Checksum:ⁱ7B5D4ACAEF97C4F1

Isoform Short (identifier: P50540-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-36: Missing.

« Hide

        10         20         30         40         50
MPSPRLQHSK PPRRLSRAQK HSSGSSNTST ANRSTHNELE KNRRAHLRLC 
        60         70         80         90        100
LERLKVLIPL GPDCTRHTTL GLLNKAKAHI KKLEEAERKS QHQLENLERE 
       110        120        130        140        150
QRFLKRRLEQ LQGPQEMERI RMDSIGSTIS SDRSDSEREE IEVDVESTEF 
       160        170        180        190 
SHGEADSVST TSISDLDDHS SLQSVGSDEG YSSASVKLSF AS

Show »

Length:192

Mass (Da):21,655

Checksum:ⁱ23D10742E4F323CF

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	Q3U3X2	Q3U3X2_MOUSE		Max interacting protein 1, isoform ... Max interacting protein 1, isoform CRA_b (Max-interacting protein 1)	Mxi1 mCG_20863	295	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	1 – 25	MERVR…RRERE → MEKHINTFLQNVQILLEAAS YLEQIEKENKK (PubMed:7835718).CuratedAdd BLAST		25

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_002141	1 – 36	Missing in isoform Short. 2 PublicationsAdd BLAST		36

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L38822 mRNA Translation: AAA65684.1 L38821 mRNA Translation: AAA65685.1 U24674 mRNA Translation: AAA65183.1 U24673 mRNA Translation: AAA65182.1 D31824 mRNA Translation: BAA06612.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS29901.1 [P50540-1] CCDS38023.1 [P50540-2]
PIRⁱ	A56069 JC4050
NCBI Reference Sequences More...RefSeqⁱ	NP_001008543.1, NM_001008543.2 [P50540-2] NP_034977.1, NM_010847.3 [P50540-1] XP_006526801.1, XM_006526738.1 XP_006526802.1, XM_006526739.1 [P50540-2] XP_006526803.1, XM_006526740.1
UniGeneⁱ	Mm.2154

Genome annotation databases

Ensemblⁱ	ENSMUST00000025998; ENSMUSP00000025998; ENSMUSG00000025025 [P50540-1] ENSMUST00000111737; ENSMUSP00000107366; ENSMUSG00000025025 [P50540-2]
GeneIDⁱ	17859
KEGGⁱ	mmu:17859
UCSC genome browser More...UCSCⁱ	uc008hwq.1 mouse [P50540-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P50540	MAX interacting protein 1		MOUSE		228	UniRef100_P50540

Protein	Similar proteins		Species	Score	Length	Source
P50540	MAX interacting protein 1		MOUSE		228	UniRef90_P50540
Max-interacting protein 1		CAMFR		254
Max-interacting protein 1 isoform a		CALJA		234
Uncharacterized protein		CAVPO		229
Uncharacterized protein		SHEEP		228
+33

Protein	Similar proteins		Species	Score	Length	Source
P50540	Max-interacting protein 1		HUMAN		228	UniRef50_P50539
Max-interacting protein 1		RAT		228
Max-interacting protein 1		DANRE		243
MAX interactor 1, dimerization protein		ORNAN		259
Max-interacting protein 1 isoform b		HETGA		228
+371

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L38822 mRNA Translation: AAA65684.1 L38821 mRNA Translation: AAA65685.1 U24674 mRNA Translation: AAA65183.1 U24673 mRNA Translation: AAA65182.1 D31824 mRNA Translation: BAA06612.1
CCDSⁱ	CCDS29901.1 [P50540-1] CCDS38023.1 [P50540-2]
PIRⁱ	A56069 JC4050
RefSeqⁱ	NP_001008543.1, NM_001008543.2 [P50540-2] NP_034977.1, NM_010847.3 [P50540-1] XP_006526801.1, XM_006526738.1 XP_006526802.1, XM_006526739.1 [P50540-2] XP_006526803.1, XM_006526740.1
UniGeneⁱ	Mm.2154

3D structure databases

ProteinModelPortalⁱ	P50540
SMRⁱ	P50540
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201630, 5 interactors
DIPⁱ	DIP-489N
IntActⁱ	P50540, 1 interactor
STRINGⁱ	10090.ENSMUSP00000003870

PTM databases

PhosphoSitePlusⁱ	P50540

PhosphoSitePlusⁱ

P50540

Proteomic databases

PaxDbⁱ	P50540
PRIDEⁱ	P50540

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000025998; ENSMUSP00000025998; ENSMUSG00000025025 [P50540-1] ENSMUST00000111737; ENSMUSP00000107366; ENSMUSG00000025025 [P50540-2]
GeneIDⁱ	17859
KEGGⁱ	mmu:17859
UCSCⁱ	uc008hwq.1 mouse [P50540-1]

Organism-specific databases

CTDⁱ	4601
MGIⁱ	MGI:97245 Mxi1

Phylogenomic databases

eggNOGⁱ	ENOG410IPD5 Eukaryota ENOG4111MIN LUCA
GeneTreeⁱ	ENSGT00510000046360
HOGENOMⁱ	HOG000247060
HOVERGENⁱ	HBG006314
InParanoidⁱ	P50540
KOⁱ	K09114
OMAⁱ	GLQDPKP
PhylomeDBⁱ	P50540

Miscellaneous databases

ChiTaRSⁱ	Mxi1 mouse
Protein Ontology More...PROⁱ	PR:P50540
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000025025 Expressed in 303 organ(s), highest expression level in blood
CleanExⁱ	MM_MXI1
ExpressionAtlasⁱ	P50540 baseline and differential
Genevisibleⁱ	P50540 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MXI1_MOUSE
Accessionⁱ	P50540Primary (citable) accession number: P50540
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	November 7, 2018
	This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P50540 (MXI1_MOUSE)

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Max-interacting protein 1

Mxi1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ