UniProtKB - P50540 (MXI1_MOUSE)
Max-interacting protein 1
Mxi1
Functioni
Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Isoform Short, which lacks a segment, has a much stronger suppressive potential and associates with a SIN3 homologous protein.
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- blastocyst formation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Max-interacting protein 1Short name: Max interactor 1 |
Gene namesi | Name:Mxi1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97245, Mxi1 |
VEuPathDBi | HostDB:ENSMUSG00000025025 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127286 | 1 – 228 | Max-interacting protein 1Add BLAST | 228 |
Proteomic databases
PaxDbi | P50540 |
PRIDEi | P50540 |
ProteomicsDBi | 287644 [P50540-1] 287645 [P50540-2] |
PTM databases
PhosphoSitePlusi | P50540 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000025025, Expressed in blood and 322 other tissues |
ExpressionAtlasi | P50540, baseline and differential |
Genevisiblei | P50540, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.
Interacts with SMC3.
Interacts with RNF17.
2 PublicationsGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 201630, 8 interactors |
DIPi | DIP-489N |
IntActi | P50540, 2 interactors |
STRINGi | 10090.ENSMUSP00000003870 |
Miscellaneous databases
RNActi | P50540, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 67 – 119 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 30 – 76 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 160 – 228 | DisorderedSequence analysisAdd BLAST | 69 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 54 – 71 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 168 – 187 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 188 – 228 | Polar residuesSequence analysisAdd BLAST | 41 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000155809 |
HOGENOMi | CLU_082604_0_1_1 |
InParanoidi | P50540 |
OMAi | AQKHSNG |
PhylomeDBi | P50540 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MERVRMINVQ RLLEAAEFLE RRERECEHGY ASSFPSMPSP RLQHSKPPRR
60 70 80 90 100
LSRAQKHSSG SSNTSTANRS THNELEKNRR AHLRLCLERL KVLIPLGPDC
110 120 130 140 150
TRHTTLGLLN KAKAHIKKLE EAERKSQHQL ENLEREQRFL KRRLEQLQGP
160 170 180 190 200
QEMERIRMDS IGSTISSDRS DSEREEIEVD VESTEFSHGE ADSVSTTSIS
210 220
DLDDHSSLQS VGSDEGYSSA SVKLSFAS
The sequence of this isoform differs from the canonical sequence as follows:
1-36: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3U3X2 | Q3U3X2_MOUSE | Max-interacting protein 1 | Mxi1 | 295 | Annotation score: | ||
A0A494B9V1 | A0A494B9V1_MOUSE | Max-interacting protein 1 | Mxi1 | 182 | Annotation score: | ||
A0A494B9I5 | A0A494B9I5_MOUSE | Max-interacting protein 1 | Mxi1 | 84 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 25 | MERVR…RRERE → MEKHINTFLQNVQILLEAAS YLEQIEKENKK (PubMed:7835718).CuratedAdd BLAST | 25 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002141 | 1 – 36 | Missing in isoform Short. 2 PublicationsAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38822 mRNA Translation: AAA65684.1 L38821 mRNA Translation: AAA65685.1 U24674 mRNA Translation: AAA65183.1 U24673 mRNA Translation: AAA65182.1 D31824 mRNA Translation: BAA06612.1 |
CCDSi | CCDS29901.1 [P50540-1] CCDS38023.1 [P50540-2] |
PIRi | A56069 JC4050 |
RefSeqi | NP_001008543.1, NM_001008543.2 [P50540-2] NP_034977.1, NM_010847.3 [P50540-1] XP_006526801.1, XM_006526738.1 XP_006526802.1, XM_006526739.1 [P50540-2] XP_006526803.1, XM_006526740.1 |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38822 mRNA Translation: AAA65684.1 L38821 mRNA Translation: AAA65685.1 U24674 mRNA Translation: AAA65183.1 U24673 mRNA Translation: AAA65182.1 D31824 mRNA Translation: BAA06612.1 |
CCDSi | CCDS29901.1 [P50540-1] CCDS38023.1 [P50540-2] |
PIRi | A56069 JC4050 |
RefSeqi | NP_001008543.1, NM_001008543.2 [P50540-2] NP_034977.1, NM_010847.3 [P50540-1] XP_006526801.1, XM_006526738.1 XP_006526802.1, XM_006526739.1 [P50540-2] XP_006526803.1, XM_006526740.1 |
3D structure databases
SMRi | P50540 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201630, 8 interactors |
DIPi | DIP-489N |
IntActi | P50540, 2 interactors |
STRINGi | 10090.ENSMUSP00000003870 |
PTM databases
PhosphoSitePlusi | P50540 |
Proteomic databases
PaxDbi | P50540 |
PRIDEi | P50540 |
ProteomicsDBi | 287644 [P50540-1] 287645 [P50540-2] |
Protocols and materials databases
Antibodypediai | 18346, 249 antibodies from 30 providers |
DNASUi | 17859 |
Genome annotation databases
Organism-specific databases
CTDi | 4601 |
MGIi | MGI:97245, Mxi1 |
VEuPathDBi | HostDB:ENSMUSG00000025025 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000155809 |
HOGENOMi | CLU_082604_0_1_1 |
InParanoidi | P50540 |
OMAi | AQKHSNG |
PhylomeDBi | P50540 |
Miscellaneous databases
BioGRID-ORCSi | 17859, 1 hit in 65 CRISPR screens |
ChiTaRSi | Mxi1, mouse |
PROi | PR:P50540 |
RNActi | P50540, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025025, Expressed in blood and 322 other tissues |
ExpressionAtlasi | P50540, baseline and differential |
Genevisiblei | P50540, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MXI1_MOUSE | |
Accessioni | P50540Primary (citable) accession number: P50540 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot