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UniProtKB - P50521 (HXK2_SCHPO)
Protein
Hexokinase-2
Gene
hxk2
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalyzes the phosphorylation of hexose (six-carbon sugars) to hexose 6-phosphate (PubMed:8549830, PubMed:9790975).
Phosphorylates D-glucose, D-fructose and D-mannose (PubMed:8549830, PubMed:9790975).
Compared to hxk1, has a much higher affinity for D-glucose (PubMed:8549830, PubMed:9790975).
Constitutes the initial enzyme of glycolysis by catalyzing the phosphorylation of glucose to D-glucose 6-phosphate (Probable).
Curated2 PublicationsCatalytic activityi
- EC:2.7.1.11 Publication1 Publication
- EC:2.7.1.11 Publication1 Publication
- EC:2.7.1.11 Publication1 Publication
- EC:2.7.1.11 Publication1 Publication
Kineticsi
- KM=0.16 mM for D-glucose1 Publication
: hexose metabolism Pathwayi
This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.
Pathwayi: glycolysis
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.1 Publication This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 222 | ATPBy similarity | 1 | |
Binding sitei | 225 | SubstrateBy similarity | 1 | |
Binding sitei | 252 | SubstrateBy similarity | 1 | |
Binding sitei | 283 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 68 – 73 | ATPBy similarity | 6 | |
Nucleotide bindingi | 288 – 289 | ATPBy similarity | 2 | |
Nucleotide bindingi | 325 – 329 | ATPBy similarity | 5 | |
Nucleotide bindingi | 400 – 404 | ATPBy similarity | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- fructokinase activity Source: PomBase
- glucokinase activity Source: PomBase
- glucose binding Source: InterPro
- mannokinase activity Source: PomBase
GO - Biological processi
- canonical glycolysis Source: PomBase
- cellular glucose homeostasis Source: GO_Central
- fructose 6-phosphate metabolic process Source: PomBase
- glucose 6-phosphate metabolic process Source: PomBase
- glycolytic fermentation Source: PomBase
- glycolytic process Source: GO_Central
- mannose metabolic process Source: PomBase
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycolysis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.1.1, 5613 |
Reactomei | R-SPO-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-SPO-6798695, Neutrophil degranulation R-SPO-70171, Glycolysis |
SABIO-RKi | P50521 |
UniPathwayi | UPA00109;UER00180 UPA00242 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hxk21 Publication ORF Names:SPAC4F8.07c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC4F8.07c, hxk2 |
VEuPathDBi | FungiDB:SPAC4F8.07c |
Subcellular locationi
Cytosol
- cytosol Source: PomBase
Mitochondrion
- mitochondrion Source: GO_Central
Nucleus
- nucleus Source: PomBase
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 196 | N → S: Decreased affinity for D-glucose. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197610 | 1 – 455 | Hexokinase-2Add BLAST | 455 |
Proteomic databases
MaxQBi | P50521 |
PaxDbi | P50521 |
PRIDEi | P50521 |
PTM databases
iPTMneti | P50521 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 280022, 10 interactors |
STRINGi | 4896.SPAC4F8.07c.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 445 | HexokinasePROSITE-ProRule annotationAdd BLAST | 443 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 57 – 195 | Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST | 139 | |
Regioni | 144 – 145 | Substrate bindingBy similarity | 2 | |
Regioni | 161 – 162 | Substrate bindingBy similarity | 2 | |
Regioni | 196 – 434 | Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST | 239 | |
Regioni | 196 – 197 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1369, Eukaryota |
HOGENOMi | CLU_014393_5_0_1 |
InParanoidi | P50521 |
OMAi | VCSIDLH |
PhylomeDBi | P50521 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR001312, Hexokinase IPR019807, Hexokinase_BS IPR022673, Hexokinase_C IPR022672, Hexokinase_N |
PANTHERi | PTHR19443, PTHR19443, 1 hit |
Pfami | View protein in Pfam PF00349, Hexokinase_1, 1 hit PF03727, Hexokinase_2, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00378, HEXOKINASE_1, 1 hit PS51748, HEXOKINASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P50521-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEANFQQAVK KLVNDFEYPT ESLREAVKEF DELRQKGLQK NGEVLAMAPA
60 70 80 90 100
FISTLPTGAE TGDFLALDFG GTNLRVCWIQ LLGDGKYEMK HSKSVLPREC
110 120 130 140 150
VRNESVKPII DFMSDHVELF IKEHFPSKFG CPEEEYLPMG FTFSYPANQV
160 170 180 190 200
SITESYLLRW TKGLNIPEAI NKDFAQFLTE GFKARNLPIR IEAVINDTVG
210 220 230 240 250
TLVTRAYTSK ESDTFMGIIF GTGTNGAYVE QMNQIPKLAG KCTGDHMLIN
260 270 280 290 300
MEWGATDFSC LHSTRYDLLL DHDTPNAGRQ IFEKRVGGMY LGELFRRALF
310 320 330 340 350
HLIKVYNFNE GIFPPSITDA WSLETSVLSR MMVERSAENV RNVLSTFKFR
360 370 380 390 400
FRSDEEALYL WDAAHAIGRR AARMSAVPIA SLYLSTGRAG KKSDVGVDGS
410 420 430 440 450
LVEHYPHFVD MLREALRELI GDNEKLISIG IAKDGSGIGA ALCALQAVKE
KKGLA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 295 | F → S in BAA13859 (PubMed:9501991).Curated | 1 | |
Sequence conflicti | 298 | A → V in BAA13859 (PubMed:9501991).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92895 mRNA Translation: CAA63488.1 CU329670 Genomic DNA Translation: CAB11054.1 D89198 mRNA Translation: BAA13859.1 |
PIRi | S68693 T42997 |
RefSeqi | NP_593865.1, NM_001019294.2 |
Genome annotation databases
EnsemblFungii | SPAC4F8.07c.1; SPAC4F8.07c.1:pep; SPAC4F8.07c |
GeneIDi | 2543607 |
KEGGi | spo:SPAC4F8.07c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92895 mRNA Translation: CAA63488.1 CU329670 Genomic DNA Translation: CAB11054.1 D89198 mRNA Translation: BAA13859.1 |
PIRi | S68693 T42997 |
RefSeqi | NP_593865.1, NM_001019294.2 |
3D structure databases
SMRi | P50521 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 280022, 10 interactors |
STRINGi | 4896.SPAC4F8.07c.1 |
PTM databases
iPTMneti | P50521 |
Proteomic databases
MaxQBi | P50521 |
PaxDbi | P50521 |
PRIDEi | P50521 |
Genome annotation databases
EnsemblFungii | SPAC4F8.07c.1; SPAC4F8.07c.1:pep; SPAC4F8.07c |
GeneIDi | 2543607 |
KEGGi | spo:SPAC4F8.07c |
Organism-specific databases
PomBasei | SPAC4F8.07c, hxk2 |
VEuPathDBi | FungiDB:SPAC4F8.07c |
Phylogenomic databases
eggNOGi | KOG1369, Eukaryota |
HOGENOMi | CLU_014393_5_0_1 |
InParanoidi | P50521 |
OMAi | VCSIDLH |
PhylomeDBi | P50521 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00180 UPA00242 |
BRENDAi | 2.7.1.1, 5613 |
Reactomei | R-SPO-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-SPO-6798695, Neutrophil degranulation R-SPO-70171, Glycolysis |
SABIO-RKi | P50521 |
Miscellaneous databases
PROi | PR:P50521 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR001312, Hexokinase IPR019807, Hexokinase_BS IPR022673, Hexokinase_C IPR022672, Hexokinase_N |
PANTHERi | PTHR19443, PTHR19443, 1 hit |
Pfami | View protein in Pfam PF00349, Hexokinase_1, 1 hit PF03727, Hexokinase_2, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00378, HEXOKINASE_1, 1 hit PS51748, HEXOKINASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HXK2_SCHPO | |
Accessioni | P50521Primary (citable) accession number: P50521 Secondary accession number(s): P78848 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families