UniProtKB - P50481 (LHX3_MOUSE)
Protein
LIM/homeobox protein Lhx3
Gene
Lhx3
Organism
Mus musculus (Mouse)
Status
Functioni
Required for the establishment of the specialized cells of the pituitary gland and the nervous system (By similarity). Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1. Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pou1f1/Pit-1.By similarity2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 160 – 219 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II transcription factor binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell differentiation Source: MGI
- dorsal/ventral pattern formation Source: MGI
- inner ear development Source: Ensembl
- lung development Source: Ensembl
- medial motor column neuron differentiation Source: MGI
- motor neuron axon guidance Source: MGI
- negative regulation of apoptotic process Source: MGI
- neuron differentiation Source: GO_Central
- pituitary gland development Source: MGI
- placenta development Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- spinal cord association neuron differentiation Source: MGI
- spinal cord motor neuron cell fate specification Source: MGI
- ventral spinal cord interneuron specification Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: LIM/homeobox protein Lhx3Short name: LIM homeobox protein 3 Alternative name(s): Homeobox protein LIM-3 Homeobox protein P-LIM |
Gene namesi | Name:Lhx3 Synonyms:Lim-3, Lim3, Plim |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102673, Lhx3 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075782 | 1 – 400 | LIM/homeobox protein Lhx3Add BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 74 | PhosphoserineBy similarity | 1 | |
Modified residuei | 230 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 237 | PhosphoserineBy similarity | 1 | |
Modified residuei | 241 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P50481 |
PRIDEi | P50481 |
ProteomicsDBi | 252472 [P50481-1] 252473 [P50481-2] |
PTM databases
PhosphoSitePlusi | P50481 |
Expressioni
Tissue specificityi
Mostly expressed in the pituitary anterior and intermediate lobes of the adult mouse. It is also expressed in the pineal gland and transiently in the primordia of motor neurons including the spinal cord, pons and medulla oblongata.
Developmental stagei
Expressed throughout pituitary development. Detected at 11 dpc in the primordium of the hypophysis. Following a maximum between 12 dpc and 14 dpc, lower levels persisted into adulthood.
Gene expression databases
Bgeei | ENSMUSG00000026934, Expressed in head and 99 other tissues |
ExpressionAtlasi | P50481, baseline and differential |
Genevisiblei | P50481, MM |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsGO - Molecular functioni
- RNA polymerase II transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201156, 8 interactors |
CORUMi | P50481 |
IntActi | P50481, 2 interactors |
MINTi | P50481 |
STRINGi | 10090.ENSMUSP00000028302 |
Miscellaneous databases
RNActi | P50481, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P50481 |
SMRi | P50481 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P50481 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 84 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Domaini | 93 – 147 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 55 |
Domaini
The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
Keywords - Domaini
Homeobox, LIM domain, RepeatPhylogenomic databases
eggNOGi | KOG4577, Eukaryota |
GeneTreei | ENSGT00940000160316 |
HOGENOMi | CLU_027802_5_0_1 |
InParanoidi | P50481 |
OMAi | LCPFPRS |
TreeFami | TF315442 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
IDEALi | IID50046 |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00412, LIM, 2 hits |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00132, LIM, 2 hits |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
Isoform LIM3A (identifier: P50481-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLLEAELDCH RERPGAPGAS ALCTFSRTPE IPMCAGCDQH ILDRFILKAL
60 70 80 90 100
DRHWHSKCLK CSDCHVPLAE RCFSRGESVY CKDDFFKRFG TKCAACQLGI
110 120 130 140 150
PPTQVVRRAQ DFVYHLHCFA CVVCKRQLAT GDEFYLMEDS RLVCKADYET
160 170 180 190 200
AKQREAEATA KRPRTTITAK QLETLKSAYN TSPKPARHVR EQLSSETGLD
210 220 230 240 250
MRVVQVWFQN RRAKEKRLKK DAGRQRWGQY FRNMKRSRGS SKSDKDSIQE
260 270 280 290 300
GQDSDAEVSF TDEPSMADMG PANGLYSSLG EPAPALGRPV GGLGSFTLDH
310 320 330 340 350
GGLTGPEQYR ELRPGSPYGI PPSPAAPQSL PGPQPLLSSL VYPDTNLSLV
360 370 380 390 400
PSGPPGGPPP MRVLAGNGPS SDLSTESSSG YPDFPASPAS WLDEVDHAQF
Isoform LIM3B (identifier: P50481-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-29: MLLEAELDCHRERPGAPGASALCTFSRTP → MEARGELDPSRESAGGDLLLALLARRADLRR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | S → N in AAA98998 (PubMed:7626792).Curated | 1 | |
Sequence conflicti | 76 | G → R in AAA73902 (PubMed:7708713).Curated | 1 | |
Sequence conflicti | 311 – 312 | EL → DV in AAA73902 (PubMed:7708713).Curated | 2 | |
Sequence conflicti | 351 – 378 | PSGPP…STESS → LQGPQVDPGPTHEGCWLEMA RTCPQRAG (PubMed:7708713).CuratedAdd BLAST | 28 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003108 | 1 – 29 | MLLEA…FSRTP → MEARGELDPSRESAGGDLLL ALLARRADLRR in isoform LIM3B. CuratedAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33776 Genomic DNA Translation: AAA62369.1 L38857 mRNA Translation: AAA73902.1 L38249 mRNA Translation: AAA98998.1 L38248 mRNA Translation: AAB64178.1 BC150689 mRNA Translation: AAI50690.1 |
CCDSi | CCDS38081.1 [P50481-2] CCDS71004.1 [P50481-1] |
PIRi | I59360 |
RefSeqi | NP_001034742.1, NM_001039653.2 [P50481-2] NP_034841.2, NM_010711.2 [P50481-1] |
Genome annotation databases
Ensembli | ENSMUST00000028302; ENSMUSP00000028302; ENSMUSG00000026934 [P50481-2] ENSMUST00000054099; ENSMUSP00000056822; ENSMUSG00000026934 [P50481-1] |
GeneIDi | 16871 |
KEGGi | mmu:16871 |
UCSCi | uc008iug.1, mouse [P50481-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33776 Genomic DNA Translation: AAA62369.1 L38857 mRNA Translation: AAA73902.1 L38249 mRNA Translation: AAA98998.1 L38248 mRNA Translation: AAB64178.1 BC150689 mRNA Translation: AAI50690.1 |
CCDSi | CCDS38081.1 [P50481-2] CCDS71004.1 [P50481-1] |
PIRi | I59360 |
RefSeqi | NP_001034742.1, NM_001039653.2 [P50481-2] NP_034841.2, NM_010711.2 [P50481-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JTN | NMR | - | A | 28-153 | [»] | |
2RGT | X-ray | 2.05 | A/B | 28-153 | [»] | |
SASBDBi | P50481 | |||||
SMRi | P50481 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201156, 8 interactors |
CORUMi | P50481 |
IntActi | P50481, 2 interactors |
MINTi | P50481 |
STRINGi | 10090.ENSMUSP00000028302 |
PTM databases
PhosphoSitePlusi | P50481 |
Proteomic databases
PaxDbi | P50481 |
PRIDEi | P50481 |
ProteomicsDBi | 252472 [P50481-1] 252473 [P50481-2] |
Protocols and materials databases
Antibodypediai | 18678, 146 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028302; ENSMUSP00000028302; ENSMUSG00000026934 [P50481-2] ENSMUST00000054099; ENSMUSP00000056822; ENSMUSG00000026934 [P50481-1] |
GeneIDi | 16871 |
KEGGi | mmu:16871 |
UCSCi | uc008iug.1, mouse [P50481-1] |
Organism-specific databases
CTDi | 8022 |
MGIi | MGI:102673, Lhx3 |
Phylogenomic databases
eggNOGi | KOG4577, Eukaryota |
GeneTreei | ENSGT00940000160316 |
HOGENOMi | CLU_027802_5_0_1 |
InParanoidi | P50481 |
OMAi | LCPFPRS |
TreeFami | TF315442 |
Miscellaneous databases
BioGRID-ORCSi | 16871, 0 hits in 53 CRISPR screens |
EvolutionaryTracei | P50481 |
PROi | PR:P50481 |
RNActi | P50481, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026934, Expressed in head and 99 other tissues |
ExpressionAtlasi | P50481, baseline and differential |
Genevisiblei | P50481, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
IDEALi | IID50046 |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00412, LIM, 2 hits |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00132, LIM, 2 hits |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LHX3_MOUSE | |
Accessioni | P50481Primary (citable) accession number: P50481 Secondary accession number(s): A2ALD9, Q61800, Q61801 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references