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Protein

Protein mlc

Gene

mlc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi247Zinc1
Metal bindingi257Zinc1
Metal bindingi259Zinc1
Metal bindingi264Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi33 – 42H-T-H motifBy similarity10

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCarbohydrate metabolism, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:PD01896

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mlc
Alternative name(s):
Making large colonies protein
Gene namesi
Name:mlc
Ordered Locus Names:b1594, JW1586
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13156 mlc

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257C → A or S: Strongly reduced activity. 1 Publication1
Mutagenesisi259C → A or S: Strongly reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000956901 – 406Protein mlcAdd BLAST406

Proteomic databases

PaxDbiP50456
PRIDEiP50456

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259119, 23 interactors
DIPiDIP-10219N
IntActiP50456, 9 interactors
STRINGi316385.ECDH10B_1727

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP50456
SMRiP50456
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50456

Family & Domainsi

Sequence similaritiesi

Belongs to the ROK (NagC/XylR) family.Curated

Phylogenomic databases

eggNOGiENOG4105DAG Bacteria
COG1940 LUCA
HOGENOMiHOG000275182
InParanoidiP50456
KOiK15545
PhylomeDBiP50456

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000600 ROK
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR18964 PTHR18964, 1 hit
PfamiView protein in Pfam
PF00480 ROK, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

P50456-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVAENQPGHI DQIKQTNAGA VYRLIDQLGP VSRIDLSRLA QLAPASITKI
60 70 80 90 100
VREMLEAHLV QELEIKEAGN RGRPAVGLVV ETEAWHYLSL RISRGEIFLA
110 120 130 140 150
LRDLSSKLVV EESQELALKD DLPLLDRIIS HIDQFFIRHQ KKLERLTSIA
160 170 180 190 200
ITLPGIIDTE NGIVHRMPFY EDVKEMPLGE ALEQHTGVPV YIQHDISAWT
210 220 230 240 250
MAEALFGASR GARDVIQVVI DHNVGAGVIT DGHLLHAGSS SLVEIGHTQV
260 270 280 290 300
DPYGKRCYCG NHGCLETIAS VDSILELAQL RLNQSMSSML HGQPLTVDSL
310 320 330 340 350
CQAALRGDLL AKDIITGVGA HVGRILAIMV NLFNPQKILI GSPLSKAADI
360 370 380 390 400
LFPVISDSIR QQALPAYSQH ISVESTQFSN QGTMAGAALV KDAMYNGSLL

IRLLQG
Length:406
Mass (Da):44,316
Last modified:November 1, 1997 - v2
Checksum:i423608797FC980CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179G → A in BAA06978 (PubMed:7766024).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32222 Genomic DNA Translation: BAA06978.1
U00096 Genomic DNA Translation: AAC74666.1
AP009048 Genomic DNA Translation: BAA15318.1
PIRiD64915
RefSeqiNP_416111.1, NC_000913.3
WP_000225262.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74666; AAC74666; b1594
BAA15318; BAA15318; BAA15318
GeneIDi945510
KEGGiecj:JW1586
eco:b1594
PATRICifig|1411691.4.peg.668

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32222 Genomic DNA Translation: BAA06978.1
U00096 Genomic DNA Translation: AAC74666.1
AP009048 Genomic DNA Translation: BAA15318.1
PIRiD64915
RefSeqiNP_416111.1, NC_000913.3
WP_000225262.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6RX-ray2.70A/B/C/D1-406[»]
3BP8X-ray2.85A/B1-406[»]
ProteinModelPortaliP50456
SMRiP50456
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259119, 23 interactors
DIPiDIP-10219N
IntActiP50456, 9 interactors
STRINGi316385.ECDH10B_1727

Proteomic databases

PaxDbiP50456
PRIDEiP50456

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74666; AAC74666; b1594
BAA15318; BAA15318; BAA15318
GeneIDi945510
KEGGiecj:JW1586
eco:b1594
PATRICifig|1411691.4.peg.668

Organism-specific databases

EchoBASEiEB2950
EcoGeneiEG13156 mlc

Phylogenomic databases

eggNOGiENOG4105DAG Bacteria
COG1940 LUCA
HOGENOMiHOG000275182
InParanoidiP50456
KOiK15545
PhylomeDBiP50456

Enzyme and pathway databases

BioCyciEcoCyc:PD01896

Miscellaneous databases

EvolutionaryTraceiP50456
PROiPR:P50456

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000600 ROK
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR18964 PTHR18964, 1 hit
PfamiView protein in Pfam
PF00480 ROK, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMLC_ECOLI
AccessioniPrimary (citable) accession number: P50456
Secondary accession number(s): P77593
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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