UniProtKB - P50456 (MLC_ECOLI)
Protein
Protein mlc
Gene
mlc
Organism
Escherichia coli (strain K12)
Status
Functioni
Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 247 | Zinc | 1 | |
Metal bindingi | 257 | Zinc | 1 | |
Metal bindingi | 259 | Zinc | 1 | |
Metal bindingi | 264 | Zinc | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 33 – 42 | H-T-H motifBy similarity | 10 |
GO - Molecular functioni
- DNA binding Source: EcoCyc
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
- transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding |
Biological process | Carbohydrate metabolism, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:PD01896 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein mlcAlternative name(s): Making large colonies protein |
Gene namesi | Name:mlc Ordered Locus Names:b1594, JW1586 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 257 | C → A or S: Strongly reduced activity. 1 Publication | 1 | |
Mutagenesisi | 259 | C → A or S: Strongly reduced activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095690 | 1 – 406 | Protein mlcAdd BLAST | 406 |
Proteomic databases
jPOSTi | P50456 |
PaxDbi | P50456 |
PRIDEi | P50456 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Hide detailsP50456
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-1116104,EBI-1116104 |
mtfA [P76346] | 2 | EBI-1116104,EBI-1126682 |
ptsG [P69786] | 3 | EBI-1116104,EBI-903497 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259119, 23 interactors |
ComplexPortali | CPX-2242, mlc-EIIB transcriptional regulator complex |
DIPi | DIP-10219N |
IntActi | P50456, 9 interactors |
STRINGi | 511145.b1594 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P50456 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P50456 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ROK (NagC/XylR) family.Curated
Phylogenomic databases
eggNOGi | COG1940, Bacteria |
HOGENOMi | CLU_036604_13_1_6 |
InParanoidi | P50456 |
PhylomeDBi | P50456 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000600, ROK IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR18964, PTHR18964, 1 hit |
Pfami | View protein in Pfam PF00480, ROK, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53067, SSF53067, 1 hit |
i Sequence
Sequence statusi: Complete.
P50456-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVAENQPGHI DQIKQTNAGA VYRLIDQLGP VSRIDLSRLA QLAPASITKI
60 70 80 90 100
VREMLEAHLV QELEIKEAGN RGRPAVGLVV ETEAWHYLSL RISRGEIFLA
110 120 130 140 150
LRDLSSKLVV EESQELALKD DLPLLDRIIS HIDQFFIRHQ KKLERLTSIA
160 170 180 190 200
ITLPGIIDTE NGIVHRMPFY EDVKEMPLGE ALEQHTGVPV YIQHDISAWT
210 220 230 240 250
MAEALFGASR GARDVIQVVI DHNVGAGVIT DGHLLHAGSS SLVEIGHTQV
260 270 280 290 300
DPYGKRCYCG NHGCLETIAS VDSILELAQL RLNQSMSSML HGQPLTVDSL
310 320 330 340 350
CQAALRGDLL AKDIITGVGA HVGRILAIMV NLFNPQKILI GSPLSKAADI
360 370 380 390 400
LFPVISDSIR QQALPAYSQH ISVESTQFSN QGTMAGAALV KDAMYNGSLL
IRLLQG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 179 | G → A in BAA06978 (PubMed:7766024).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D32222 Genomic DNA Translation: BAA06978.1 U00096 Genomic DNA Translation: AAC74666.1 AP009048 Genomic DNA Translation: BAA15318.1 |
PIRi | D64915 |
RefSeqi | NP_416111.1, NC_000913.3 WP_000225262.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74666; AAC74666; b1594 BAA15318; BAA15318; BAA15318 |
GeneIDi | 57730987 945510 |
KEGGi | ecj:JW1586 eco:b1594 |
PATRICi | fig|1411691.4.peg.668 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D32222 Genomic DNA Translation: BAA06978.1 U00096 Genomic DNA Translation: AAC74666.1 AP009048 Genomic DNA Translation: BAA15318.1 |
PIRi | D64915 |
RefSeqi | NP_416111.1, NC_000913.3 WP_000225262.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Z6R | X-ray | 2.70 | A/B/C/D | 1-406 | [»] | |
3BP8 | X-ray | 2.85 | A/B | 1-406 | [»] | |
SMRi | P50456 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259119, 23 interactors |
ComplexPortali | CPX-2242, mlc-EIIB transcriptional regulator complex |
DIPi | DIP-10219N |
IntActi | P50456, 9 interactors |
STRINGi | 511145.b1594 |
Proteomic databases
jPOSTi | P50456 |
PaxDbi | P50456 |
PRIDEi | P50456 |
Genome annotation databases
EnsemblBacteriai | AAC74666; AAC74666; b1594 BAA15318; BAA15318; BAA15318 |
GeneIDi | 57730987 945510 |
KEGGi | ecj:JW1586 eco:b1594 |
PATRICi | fig|1411691.4.peg.668 |
Organism-specific databases
EchoBASEi | EB2950 |
Phylogenomic databases
eggNOGi | COG1940, Bacteria |
HOGENOMi | CLU_036604_13_1_6 |
InParanoidi | P50456 |
PhylomeDBi | P50456 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD01896 |
Miscellaneous databases
EvolutionaryTracei | P50456 |
PROi | PR:P50456 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000600, ROK IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR18964, PTHR18964, 1 hit |
Pfami | View protein in Pfam PF00480, ROK, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53067, SSF53067, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MLC_ECOLI | |
Accessioni | P50456Primary (citable) accession number: P50456 Secondary accession number(s): P77593 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families