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Protein

Serine hydroxymethyltransferase, cytosolic

Gene

Shmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interconversion of serine and glycine.By similarity

Miscellaneous

In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.Curated

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.By similarity

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-196757 Metabolism of folate and pterines
R-MMU-71262 Carnitine synthesis
UniPathwayi
UPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase, cytosolic (EC:2.1.2.1By similarity)
Short name:
SHMT
Alternative name(s):
Glycine hydroxymethyltransferase
Serine methylase
Gene namesi
Name:Shmt1
Synonyms:Shmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98299 Shmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4396

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001135051 – 478Serine hydroxymethyltransferase, cytosolicAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251N6-(pyridoxal phosphate)lysine1 Publication1

Proteomic databases

EPDiP50431
MaxQBiP50431
PaxDbiP50431
PeptideAtlasiP50431
PRIDEiP50431

2D gel databases

REPRODUCTION-2DPAGEiP50431
SWISS-2DPAGEiP50431

PTM databases

iPTMnetiP50431
PhosphoSitePlusiP50431
SwissPalmiP50431

Expressioni

Gene expression databases

BgeeiENSMUSG00000020534 Expressed in 278 organ(s), highest expression level in paneth cell
CleanExiMM_SHMT1
ExpressionAtlasiP50431 baseline and differential
GenevisibleiP50431 MM

Interactioni

Subunit structurei

Homotetramer (PubMed:11063567). Identified in complex with FAM175B and the other subunits of the BRISC complex, at least composed of FAM175B/ABRO1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP50431, 2 interactors
MINTiP50431
STRINGi10090.ENSMUSP00000018744

Structurei

Secondary structure

1478
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP50431
SMRiP50431
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50431

Family & Domainsi

Sequence similaritiesi

Belongs to the SHMT family.Curated

Phylogenomic databases

eggNOGiKOG2467 Eukaryota
COG0112 LUCA
GeneTreeiENSGT00390000002762
HOGENOMiHOG000239405
HOVERGENiHBG002807
InParanoidiP50431
KOiK00600
OMAiPLEHIIA
OrthoDBiEOG091G05AU
TreeFamiTF314667

Family and domain databases

CDDicd00378 SHMT, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00051 SHMT, 1 hit
InterProiView protein in InterPro
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR001085 Ser_HO-MeTrfase
IPR019798 Ser_HO-MeTrfase_PLP_BS
IPR039429 SHMT-like_dom
PANTHERiPTHR11680 PTHR11680, 1 hit
PfamiView protein in Pfam
PF00464 SHMT, 1 hit
PIRSFiPIRSF000412 SHMT, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00096 SHMT, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P50431-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADRDATLWA SHEKMLSQPL KDSDAEVYSI IKKESNRQRV GLELIASENF
60 70 80 90 100
ASRAVLEALG SCLNNKYSEG YPGQRYYGGT EFIDELEMLC QKRALQAYHL
110 120 130 140 150
DPQCWGVNVQ PYSGSPANFA VYTALVEPHG RIMGLDLPDG GHLTHGFMTD
160 170 180 190 200
KKKISATSIF FESMPYKVYP ETGYINYDQL EENASLFHPK LIIAGTSCYS
210 220 230 240 250
RNLDYARLRK IADDNGAYLM ADMAHISGLV AAGVVPSPFE HCHVVTTTTH
260 270 280 290 300
KTLRGCRAGM IFYRKGVRSV DPKTGKETYY ELESLINSAV FPGLQGGPHN
310 320 330 340 350
HAIAGVAVAL KQAMTTEFKI YQLQVLANCR ALSDALTELG YKIVTGGSDN
360 370 380 390 400
HLILMDLRSK GTDGGRAEKV LEACSIACNK NTCPGDKSAL RPSGLRLGTP
410 420 430 440 450
ALTSRGLLEE DFQKVAHFIH RGIELTLQIQ SHMATKATLK EFKEKLAGDE
460 470
KIQSAVATLR EEVENFASNF SLPGLPDF
Length:478
Mass (Da):52,601
Last modified:July 27, 2011 - v3
Checksum:i780C764C5AA3CEA3
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UYY1G3UYY1_MOUSE
Serine hydroxymethyltransferase
Shmt1
441Annotation score:
G3UZ26G3UZ26_MOUSE
Serine hydroxymethyltransferase
Shmt1
439Annotation score:
G3UZZ9G3UZZ9_MOUSE
Serine hydroxymethyltransferase, cy...
Shmt1
122Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62C → S in AAK15040 (PubMed:11063567).Curated1
Sequence conflicti62C → S in CAA64225 (PubMed:8863732).Curated1
Sequence conflicti62C → S in CAA64226 (PubMed:8863732).Curated1
Sequence conflicti267 – 309VRSVD…GVAVA → KFPYAGTDSVGSHFLCGRDW ENSGLSLLGKIGALALEELL RKK in CAA64225 (PubMed:8863732).CuratedAdd BLAST43
Sequence conflicti304A → R in CAA64226 (PubMed:8863732).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237702 mRNA Translation: AAK15040.1
AL596215 Genomic DNA No translation available.
BC026055 mRNA Translation: AAH26055.1
X94478 mRNA Translation: CAA64225.1
X94479 mRNA Translation: CAA64226.1
CCDSiCCDS24799.1
PIRiJC4959
RefSeqiNP_033197.2, NM_009171.2
XP_006532707.1, XM_006532644.3
UniGeneiMm.364956

Genome annotation databases

EnsembliENSMUST00000018744; ENSMUSP00000018744; ENSMUSG00000020534
GeneIDi20425
KEGGimmu:20425
UCSCiuc007jgo.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237702 mRNA Translation: AAK15040.1
AL596215 Genomic DNA No translation available.
BC026055 mRNA Translation: AAH26055.1
X94478 mRNA Translation: CAA64225.1
X94479 mRNA Translation: CAA64226.1
CCDSiCCDS24799.1
PIRiJC4959
RefSeqiNP_033197.2, NM_009171.2
XP_006532707.1, XM_006532644.3
UniGeneiMm.364956

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EJIX-ray2.90A/B/C/D1-478[»]
ProteinModelPortaliP50431
SMRiP50431
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50431, 2 interactors
MINTiP50431
STRINGi10090.ENSMUSP00000018744

Chemistry databases

ChEMBLiCHEMBL4396

PTM databases

iPTMnetiP50431
PhosphoSitePlusiP50431
SwissPalmiP50431

2D gel databases

REPRODUCTION-2DPAGEiP50431
SWISS-2DPAGEiP50431

Proteomic databases

EPDiP50431
MaxQBiP50431
PaxDbiP50431
PeptideAtlasiP50431
PRIDEiP50431

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018744; ENSMUSP00000018744; ENSMUSG00000020534
GeneIDi20425
KEGGimmu:20425
UCSCiuc007jgo.1 mouse

Organism-specific databases

CTDi6470
MGIiMGI:98299 Shmt1

Phylogenomic databases

eggNOGiKOG2467 Eukaryota
COG0112 LUCA
GeneTreeiENSGT00390000002762
HOGENOMiHOG000239405
HOVERGENiHBG002807
InParanoidiP50431
KOiK00600
OMAiPLEHIIA
OrthoDBiEOG091G05AU
TreeFamiTF314667

Enzyme and pathway databases

UniPathwayi
UPA00193

ReactomeiR-MMU-196757 Metabolism of folate and pterines
R-MMU-71262 Carnitine synthesis

Miscellaneous databases

EvolutionaryTraceiP50431
PROiPR:P50431
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020534 Expressed in 278 organ(s), highest expression level in paneth cell
CleanExiMM_SHMT1
ExpressionAtlasiP50431 baseline and differential
GenevisibleiP50431 MM

Family and domain databases

CDDicd00378 SHMT, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00051 SHMT, 1 hit
InterProiView protein in InterPro
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR001085 Ser_HO-MeTrfase
IPR019798 Ser_HO-MeTrfase_PLP_BS
IPR039429 SHMT-like_dom
PANTHERiPTHR11680 PTHR11680, 1 hit
PfamiView protein in Pfam
PF00464 SHMT, 1 hit
PIRSFiPIRSF000412 SHMT, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00096 SHMT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLYC_MOUSE
AccessioniPrimary (citable) accession number: P50431
Secondary accession number(s): Q64508, Q8R0X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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