UniProtKB - P50429 (ARSB_MOUSE)
Arylsulfatase B
Arsb
Functioni
Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (By similarity).
Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium (By similarity).
In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity).
By similarityCatalytic activityi
- Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate. EC:3.1.6.12
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 54 | CalciumBy similarity | 1 | |
Metal bindingi | 55 | CalciumBy similarity | 1 | |
Active sitei | 92 | NucleophileBy similarity | 1 | |
Metal bindingi | 92 | Calcium; via 3-oxoalanineBy similarity | 1 | |
Binding sitei | 146 | SubstrateBy similarity | 1 | |
Active sitei | 148 | By similarity | 1 | |
Binding sitei | 243 | SubstrateBy similarity | 1 | |
Metal bindingi | 301 | CalciumBy similarity | 1 | |
Metal bindingi | 302 | CalciumBy similarity | 1 | |
Binding sitei | 319 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- arylsulfatase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- N-acetylgalactosamine-4-sulfatase activity Source: UniProtKB
- sulfuric ester hydrolase activity Source: MGI
GO - Biological processi
- anterior head development Source: MGI
- autophagy Source: MGI
- central nervous system development Source: MGI
- colon epithelial cell migration Source: UniProtKB
- positive regulation of neuron projection development Source: UniProtKB
- regulation of epithelial cell migration Source: UniProtKB
- response to estrogen Source: MGI
- response to methylmercury Source: MGI
- response to nutrient Source: MGI
- response to pH Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.6.12, 3474 |
Reactomei | R-MMU-1660662, Glycosphingolipid metabolism R-MMU-1663150, The activation of arylsulfatases R-MMU-2024101, CS/DS degradation R-MMU-6798695, Neutrophil degranulation |
SABIO-RKi | P50429 |
Names & Taxonomyi
Protein namesi | Recommended name: Arylsulfatase B (EC:3.1.6.12)Short name: ASB Alternative name(s): N-acetylgalactosamine-4-sulfatase Short name: G4S |
Gene namesi | Name:Arsb Synonyms:As1, As1-s |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88075, Arsb |
Subcellular locationi
Lysosome
- Lysosome 1 Publication
Other locations
- Cell surface 1 Publication
Endoplasmic reticulum
- rough endoplasmic reticulum Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Lysosome
- lysosome Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
- cell surface Source: UniProtKB
Keywords - Cellular componenti
LysosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 41 | Sequence analysisAdd BLAST | 41 | |
ChainiPRO_0000033423 | 42 – 534 | Arylsulfatase BAdd BLAST | 493 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 92 | 3-oxoalanine (Cys)By similarity | 1 | |
Disulfide bondi | 118 ↔ 522 | By similarity | ||
Disulfide bondi | 122 ↔ 156 | By similarity | ||
Disulfide bondi | 182 ↔ 193 | By similarity | ||
Glycosylationi | 189 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 280 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 292 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 367 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 406 ↔ 448 | By similarity | ||
Glycosylationi | 427 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 459 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | P50429 |
jPOSTi | P50429 |
MaxQBi | P50429 |
PaxDbi | P50429 |
PeptideAtlasi | P50429 |
PRIDEi | P50429 |
ProteomicsDBi | 281906 [P50429-1] 281907 [P50429-2] |
PTM databases
GlyConnecti | 2134, 4 N-Linked glycans (2 sites) |
GlyGeni | P50429, 6 sites, 4 N-linked glycans (2 sites) |
PhosphoSitePlusi | P50429 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 198216, 9 interactors |
STRINGi | 10090.ENSMUSP00000088964 |
Miscellaneous databases
RNActi | P50429, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3867, Eukaryota |
InParanoidi | P50429 |
OrthoDBi | 515367at2759 |
PhylomeDBi | P50429 |
TreeFami | TF314186 |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR024607, Sulfatase_CS IPR000917, Sulfatase_N |
Pfami | View protein in Pfam PF00884, Sulfatase, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00523, SULFATASE_1, 1 hit PS00149, SULFATASE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGKLSPCTGR SRPGGPGPQL PLLLLLLQLL LLLLSPARAS GATQPPHVVF
60 70 80 90 100
VLADDLGWND LGFHGSVIRT PHLDALAAGG VVLDNYYVQP LCTPSRSQLL
110 120 130 140 150
TGRYQIHLGL QHYLIMTCQP SCVPLDEKLL PQLLKEAGYA THMVGKWHLG
160 170 180 190 200
MYRKECLPTR RGFDTYFGYL LGSEDYYTHE ACAPIESLNG TRCALDLRDG
210 220 230 240 250
EEPAKEYNNI YSTNIFTKRA TTVIANHPPE KPLFLYLAFQ SVHDPLQVPE
260 270 280 290 300
EYMEPYGFIQ DKHRRIYAGM VSLMDEAVGN VTKALKSHGL WNNTVFIFST
310 320 330 340 350
DNGGQTRSGG NNWPLRGRKG TLWEGGIRGT GFVASPLLKQ KGVKSRELMH
360 370 380 390 400
ITDWLPTLVD LAGGSTNGTK PLDGFNMWKT ISEGHPSPRV ELLHNIDQDF
410 420 430 440 450
FDGLPCPGKN MTPAKDDSFP LEHSAFNTSI HAGIRYKNWK LLTGHPGCGY
460 470 480 490 500
WFPPPSQSNV SEIPPVGPPT KTLWLFDINQ DPEERHDVSR EHPHIVQNLL
510 520 530
SRLQYYHEHS VPSHFPPLDP RCDPKSTGVW SPWM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J138 | A0A0R4J138_MOUSE | Arylsulfatase B | Arsb | 534 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | D → A in AAA37261 (PubMed:1572648).Curated | 1 | |
Sequence conflicti | 352 | T → S in BAE34455 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 352 | T → S in CAA63067 (PubMed:8710849).Curated | 1 | |
Sequence conflicti | 467 | G → D in CAI84992 (PubMed:19468303).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007881 | 383 – 431 | EGHPS…NTSIH → PVTGDHWHAEGELGCSFRTA SAAEEEPTYKLREKKRRKSP DCGRARWFL in isoform 2. 1 PublicationAdd BLAST | 49 | |
Alternative sequenceiVSP_022249 | 432 – 534 | Missing in isoform 2. 1 PublicationAdd BLAST | 103 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK083309 mRNA Translation: BAC38859.1 AK154098 mRNA Translation: BAE32375.1 AK158312 mRNA Translation: BAE34455.1 AC131739 Genomic DNA No translation available. AC136976 Genomic DNA No translation available. M82877 mRNA Translation: AAA37261.1 X92096 mRNA Translation: CAA63067.1 BN000746 mRNA Translation: CAI84992.1 |
CCDSi | CCDS36749.1 [P50429-1] |
RefSeqi | NP_033842.3, NM_009712.3 |
Genome annotation databases
GeneIDi | 11881 |
KEGGi | mmu:11881 |
UCSCi | uc007rlo.1, mouse [P50429-1] uc011zcv.1, mouse [P50429-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK083309 mRNA Translation: BAC38859.1 AK154098 mRNA Translation: BAE32375.1 AK158312 mRNA Translation: BAE34455.1 AC131739 Genomic DNA No translation available. AC136976 Genomic DNA No translation available. M82877 mRNA Translation: AAA37261.1 X92096 mRNA Translation: CAA63067.1 BN000746 mRNA Translation: CAI84992.1 |
CCDSi | CCDS36749.1 [P50429-1] |
RefSeqi | NP_033842.3, NM_009712.3 |
3D structure databases
AlphaFoldDBi | P50429 |
SMRi | P50429 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198216, 9 interactors |
STRINGi | 10090.ENSMUSP00000088964 |
PTM databases
GlyConnecti | 2134, 4 N-Linked glycans (2 sites) |
GlyGeni | P50429, 6 sites, 4 N-linked glycans (2 sites) |
PhosphoSitePlusi | P50429 |
Proteomic databases
EPDi | P50429 |
jPOSTi | P50429 |
MaxQBi | P50429 |
PaxDbi | P50429 |
PeptideAtlasi | P50429 |
PRIDEi | P50429 |
ProteomicsDBi | 281906 [P50429-1] 281907 [P50429-2] |
Protocols and materials databases
DNASUi | 11881 |
Genome annotation databases
GeneIDi | 11881 |
KEGGi | mmu:11881 |
UCSCi | uc007rlo.1, mouse [P50429-1] uc011zcv.1, mouse [P50429-2] |
Organism-specific databases
CTDi | 411 |
MGIi | MGI:88075, Arsb |
Phylogenomic databases
eggNOGi | KOG3867, Eukaryota |
InParanoidi | P50429 |
OrthoDBi | 515367at2759 |
PhylomeDBi | P50429 |
TreeFami | TF314186 |
Enzyme and pathway databases
BRENDAi | 3.1.6.12, 3474 |
Reactomei | R-MMU-1660662, Glycosphingolipid metabolism R-MMU-1663150, The activation of arylsulfatases R-MMU-2024101, CS/DS degradation R-MMU-6798695, Neutrophil degranulation |
SABIO-RKi | P50429 |
Miscellaneous databases
BioGRID-ORCSi | 11881, 1 hit in 75 CRISPR screens |
ChiTaRSi | Arsb, mouse |
PROi | PR:P50429 |
RNActi | P50429, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR024607, Sulfatase_CS IPR000917, Sulfatase_N |
Pfami | View protein in Pfam PF00884, Sulfatase, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00523, SULFATASE_1, 1 hit PS00149, SULFATASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARSB_MOUSE | |
Accessioni | P50429Primary (citable) accession number: P50429 Secondary accession number(s): Q32KJ1 Q8C404 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 9, 2007 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families