UniProtKB - P50404 (SFTPD_MOUSE)
Protein
Pulmonary surfactant-associated protein D
Gene
Sftpd
Organism
Mus musculus (Mouse)
Status
Functioni
Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Miscellaneous
Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
GO - Molecular functioni
- identical protein binding Source: IntAct
- lipopolysaccharide binding Source: MGI
- monosaccharide binding Source: MGI
GO - Biological processi
- induction of bacterial agglutination Source: MGI
- innate immune response Source: UniProtKB-KW
- lung alveolus development Source: UniProtKB
- negative regulation by host of symbiont molecular function Source: MGI
- negative regulation of interleukin-2 production Source: MGI
- negative regulation of phagocytosis Source: MGI
- negative regulation of T cell proliferation Source: MGI
- opsonization Source: MGI
- positive regulation of phagocytosis Source: MGI
- regulation of adhesion of symbiont to host epithelial cell Source: MGI
- regulation of liquid surface tension Source: MGI
- respiratory gaseous exchange by respiratory system Source: UniProtKB-KW
- surfactant homeostasis Source: UniProtKB
Keywordsi
Biological process | Gaseous exchange, Immunity, Innate immunity |
Ligand | Calcium, Lectin |
Enzyme and pathway databases
Reactomei | R-MMU-166016, Toll Like Receptor 4 (TLR4) Cascade R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-391160, Signal regulatory protein family interactions R-MMU-5683826, Surfactant metabolism R-MMU-5686938, Regulation of TLR by endogenous ligand |
Names & Taxonomyi
Protein namesi | Recommended name: Pulmonary surfactant-associated protein DShort name: PSP-D Short name: SP-D Alternative name(s): Lung surfactant protein D |
Gene namesi | Name:Sftpd Synonyms:Sftp4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109515, Sftpd |
Subcellular locationi
Extracellular region or secreted
Endoplasmic reticulum
- rough endoplasmic reticulum Source: MGI
Endosome
- multivesicular body Source: MGI
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular space Source: MGI
Other locations
- collagen trimer Source: UniProtKB-KW
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
Keywords - Cellular componenti
Extracellular matrix, Secreted, Surface filmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | By similarityAdd BLAST | 19 | |
ChainiPRO_0000017466 | 20 – 374 | Pulmonary surfactant-associated protein DAdd BLAST | 355 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | S-nitrosocysteineBy similarity | 1 | |
Modified residuei | 39 | S-nitrosocysteineBy similarity | 1 | |
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 280 ↔ 372 | PROSITE-ProRule annotation | ||
Disulfide bondi | 350 ↔ 364 | PROSITE-ProRule annotation |
Post-translational modificationi
S-nitrosylation at Cys-34 and Cys-39 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, Hydroxylation, S-nitrosylationProteomic databases
CPTACi | non-CPTAC-4006 |
MaxQBi | P50404 |
PaxDbi | P50404 |
PeptideAtlasi | P50404 |
PRIDEi | P50404 |
PTM databases
GlyGeni | P50404, 1 site |
PhosphoSitePlusi | P50404 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021795, Expressed in right lung and 74 other tissues |
Genevisiblei | P50404, MM |
Interactioni
Subunit structurei
Oligomeric complex of 4 set of homotrimers.
Binary interactionsi
P50404
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-8402361,EBI-8402361 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 203194, 2 interactors |
DIPi | DIP-46338N |
IntActi | P50404, 2 interactors |
MINTi | P50404 |
STRINGi | 10090.ENSMUSP00000076383 |
Miscellaneous databases
RNActi | P50404, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 221 | Collagen-likeAdd BLAST | 177 | |
Domaini | 259 – 374 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 116 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 222 – 253 | Sequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Belongs to the SFTPD family.Curated
Keywords - Domaini
Coiled coil, Collagen, Repeat, SignalPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000155748 |
HOGENOMi | CLU_049894_3_0_1 |
InParanoidi | P50404 |
OMAi | ANTCTLV |
OrthoDBi | 1341167at2759 |
PhylomeDBi | P50404 |
TreeFami | TF330481 |
Family and domain databases
CDDi | cd03591, CLECT_collectin_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR008160, Collagen IPR033990, Collectin_CTLD IPR016187, CTDL_fold IPR015097, Surfac_D-trimer |
Pfami | View protein in Pfam PF01391, Collagen, 2 hits PF00059, Lectin_C, 1 hit PF09006, Surfac_D-trimer, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P50404-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLPFLSMLVL LVQPLGNLGA EMKSLSQRSV PNTCTLVMCS PTENGLPGRD
60 70 80 90 100
GRDGREGPRG EKGDPGLPGP MGLSGLQGPT GPVGPKGENG SAGEPGPKGE
110 120 130 140 150
RGLSGPPGLP GIPGPAGKEG PSGKQGNIGP QGKPGPKGEA GPKGEVGAPG
160 170 180 190 200
MQGSTGAKGS TGPKGERGAP GVQGAPGNAG AAGPAGPAGP QGAPGSRGPP
210 220 230 240 250
GLKGDRGVPG DRGIKGESGL PDSAALRQQM EALKGKLQRL EVAFSHYQKA
260 270 280 290 300
ALFPDGRSVG DKIFRTADSE KPFEDAQEMC KQAGGQLASP RSATENAAIQ
310 320 330 340 350
QLITAHNKAA FLSMTDVGTE GKFTYPTGEP LVYSNWAPGE PNNNGGAENC
360 370
VEIFTNGQWN DKACGEQRLV ICEF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L40156 mRNA Translation: AAA92021.1 AF047742, AF047741 Genomic DNA Translation: AAD31380.1 AF192134 Genomic DNA Translation: AAF15277.1 BC003705 mRNA Translation: AAH03705.1 |
CCDSi | CCDS26962.1 |
RefSeqi | NP_033186.1, NM_009160.2 |
Genome annotation databases
Ensembli | ENSMUST00000077136; ENSMUSP00000076383; ENSMUSG00000021795 |
GeneIDi | 20390 |
KEGGi | mmu:20390 |
UCSCi | uc007tcr.2, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding Pulmonary surfactant protein SP-D |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L40156 mRNA Translation: AAA92021.1 AF047742, AF047741 Genomic DNA Translation: AAD31380.1 AF192134 Genomic DNA Translation: AAF15277.1 BC003705 mRNA Translation: AAH03705.1 |
CCDSi | CCDS26962.1 |
RefSeqi | NP_033186.1, NM_009160.2 |
3D structure databases
SMRi | P50404 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203194, 2 interactors |
DIPi | DIP-46338N |
IntActi | P50404, 2 interactors |
MINTi | P50404 |
STRINGi | 10090.ENSMUSP00000076383 |
PTM databases
GlyGeni | P50404, 1 site |
PhosphoSitePlusi | P50404 |
Proteomic databases
CPTACi | non-CPTAC-4006 |
MaxQBi | P50404 |
PaxDbi | P50404 |
PeptideAtlasi | P50404 |
PRIDEi | P50404 |
Protocols and materials databases
Antibodypediai | 3886, 524 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000077136; ENSMUSP00000076383; ENSMUSG00000021795 |
GeneIDi | 20390 |
KEGGi | mmu:20390 |
UCSCi | uc007tcr.2, mouse |
Organism-specific databases
CTDi | 6441 |
MGIi | MGI:109515, Sftpd |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000155748 |
HOGENOMi | CLU_049894_3_0_1 |
InParanoidi | P50404 |
OMAi | ANTCTLV |
OrthoDBi | 1341167at2759 |
PhylomeDBi | P50404 |
TreeFami | TF330481 |
Enzyme and pathway databases
Reactomei | R-MMU-166016, Toll Like Receptor 4 (TLR4) Cascade R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-391160, Signal regulatory protein family interactions R-MMU-5683826, Surfactant metabolism R-MMU-5686938, Regulation of TLR by endogenous ligand |
Miscellaneous databases
BioGRID-ORCSi | 20390, 1 hit in 17 CRISPR screens |
ChiTaRSi | Sftpd, mouse |
PROi | PR:P50404 |
RNActi | P50404, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021795, Expressed in right lung and 74 other tissues |
Genevisiblei | P50404, MM |
Family and domain databases
CDDi | cd03591, CLECT_collectin_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR008160, Collagen IPR033990, Collectin_CTLD IPR016187, CTDL_fold IPR015097, Surfac_D-trimer |
Pfami | View protein in Pfam PF01391, Collagen, 2 hits PF00059, Lectin_C, 1 hit PF09006, Surfac_D-trimer, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SFTPD_MOUSE | |
Accessioni | P50404Primary (citable) accession number: P50404 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families