UniProtKB - P50395 (GDIB_HUMAN)
Protein
Rab GDP dissociation inhibitor beta
Gene
GDI2
Organism
Homo sapiens (Human)
Status
Functioni
Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB-KW
- Rab GDP-dissociation inhibitor activity Source: GO_Central
- Rab GTPase binding Source: GO_Central
- RNA binding Source: UniProtKB
GO - Biological processi
- neutrophil degranulation Source: Reactome
- protein transport Source: InterPro
- regulation of small GTPase mediated signal transduction Source: Reactome
- signal transduction Source: UniProtKB
- small GTPase mediated signal transduction Source: Ensembl
- vesicle-mediated transport Source: GO_Central
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
PathwayCommonsi | P50395 |
Reactomei | R-HSA-194840, Rho GTPase cycle R-HSA-6798695, Neutrophil degranulation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
Names & Taxonomyi
Protein namesi | Recommended name: Rab GDP dissociation inhibitor betaShort name: Rab GDI beta Alternative name(s): Guanosine diphosphate dissociation inhibitor 2 Short name: GDI-2 |
Gene namesi | Name:GDI2 Synonyms:RABGDIB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000057608.16 |
HGNCi | HGNC:4227, GDI2 |
MIMi | 600767, gene |
neXtProti | NX_P50395 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
Golgi apparatus
- Golgi apparatus Source: Ensembl
Lysosome
- azurophil granule lumen Source: Reactome
Other locations
- cytoplasm Source: GO_Central
- focal adhesion Source: UniProtKB
- membrane Source: UniProtKB-SubCell
- secretory granule lumen Source: Reactome
- vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 2665 |
OpenTargetsi | ENSG00000057608 |
PharmGKBi | PA28642 |
Miscellaneous databases
Pharosi | P50395, Tbio |
Polymorphism and mutation databases
BioMutai | GDI2 |
DMDMi | 13638228 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056679 | 1 – 445 | Rab GDP dissociation inhibitor betaAdd BLAST | 445 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 57 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 61 | PhosphoserineCombined sources | 1 | |
Modified residuei | 112 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Modified residuei | 269 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 382 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-512 CPTAC-513 |
EPDi | P50395 |
jPOSTi | P50395 |
MassIVEi | P50395 |
PaxDbi | P50395 |
PeptideAtlasi | P50395 |
PRIDEi | P50395 |
ProteomicsDBi | 56216 [P50395-1] 56217 [P50395-2] |
2D gel databases
OGPi | P50395 |
REPRODUCTION-2DPAGEi | IPI00031461 P50395 |
PTM databases
iPTMneti | P50395 |
MetOSitei | P50395 |
PhosphoSitePlusi | P50395 |
SwissPalmi | P50395 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000057608, Expressed in female gonad and 247 other tissues |
ExpressionAtlasi | P50395, baseline and differential |
Genevisiblei | P50395, HS |
Organism-specific databases
HPAi | ENSG00000057608, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP50395
GO - Molecular functioni
- Rab GTPase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 108933, 70 interactors |
DIPi | DIP-50593N |
IntActi | P50395, 45 interactors |
MINTi | P50395 |
STRINGi | 9606.ENSP00000369538 |
Miscellaneous databases
RNActi | P50395, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the Rab GDI family.Curated
Phylogenomic databases
eggNOGi | KOG1439, Eukaryota |
GeneTreei | ENSGT00950000182994 |
HOGENOMi | CLU_021695_0_0_1 |
InParanoidi | P50395 |
OMAi | PAMNEEY |
PhylomeDBi | P50395 |
TreeFami | TF300449 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR018203, GDP_dissociation_inhibitor IPR000806, RabGDI |
PANTHERi | PTHR11787, PTHR11787, 1 hit |
Pfami | View protein in Pfam PF00996, GDI, 1 hit |
PRINTSi | PR00892, RABGDI PR00891, RABGDIREP |
SUPFAMi | SSF51905, SSF51905, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P50395-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESASITPLE
60 70 80 90 100
DLYKRFKIPG SPPESMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL
110 120 130 140 150
DFKVTEGSFV YKGGKIYKVP STEAEALASS LMGLFEKRRF RKFLVYVANF
160 170 180 190 200
DEKDPRTFEG IDPKKTTMRD VYKKFDLGQD VIDFTGHALA LYRTDDYLDQ
210 220 230 240 250
PCYETINRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM
260 270 280 290 300
LNKPIEEIIV QNGKVIGVKS EGEIARCKQL ICDPSYVKDR VEKVGQVIRV
310 320 330 340 350
ICILSHPIKN TNDANSCQII IPQNQVNRKS DIYVCMISFA HNVAAQGKYI
360 370 380 390 400
AIVSTTVETK EPEKEIRPAL ELLEPIEQKF VSISDLLVPK DLGTESQIFI
410 420 430 440
SRTYDATTHF ETTCDDIKNI YKRMTGSEFD FEEMKRKKND IYGED
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5SX87 | Q5SX87_HUMAN | Rab GDP dissociation inhibitor | GDI2 | 263 | Annotation score: | ||
Q5SX91 | Q5SX91_HUMAN | Rab GDP dissociation inhibitor | GDI2 | 203 | Annotation score: | ||
Q5SX86 | Q5SX86_HUMAN | Rab GDP dissociation inhibitor | GDI2 | 155 | Annotation score: | ||
Q5SX90 | Q5SX90_HUMAN | Rab GDP dissociation inhibitor | GDI2 | 148 | Annotation score: | ||
V9GYF8 | V9GYF8_HUMAN | Rab GDP dissociation inhibitor | GDI2 | 130 | Annotation score: | ||
V9GYJ7 | V9GYJ7_HUMAN | Rab GDP dissociation inhibitor | GDI2 | 125 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | N → D in BAA03095 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043469 | 86 – 130 | Missing in isoform 2. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13988 mRNA Translation: BAA03095.1 Y13286 mRNA Translation: CAA73734.1 Y13287 , Y13288, Y13289, Y13290, Y13291, Y13292, Y13293, Y13294, Y13295, Y13296, Y13297 Genomic DNA Translation: CAA73735.1 BT006868 mRNA Translation: AAP35514.1 AK297554 mRNA Translation: BAG59947.1 AL596094 Genomic DNA No translation available. BC005145 mRNA Translation: AAH05145.1 AF144713 mRNA Translation: AAD34588.1 |
CCDSi | CCDS44352.1 [P50395-2] CCDS7071.1 [P50395-1] |
RefSeqi | NP_001108628.1, NM_001115156.1 [P50395-2] NP_001485.2, NM_001494.3 [P50395-1] |
Genome annotation databases
Ensembli | ENST00000380181; ENSP00000369528; ENSG00000057608 [P50395-2] ENST00000380191; ENSP00000369538; ENSG00000057608 [P50395-1] |
GeneIDi | 2665 |
KEGGi | hsa:2665 |
UCSCi | uc001iim.5, human [P50395-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13988 mRNA Translation: BAA03095.1 Y13286 mRNA Translation: CAA73734.1 Y13287 , Y13288, Y13289, Y13290, Y13291, Y13292, Y13293, Y13294, Y13295, Y13296, Y13297 Genomic DNA Translation: CAA73735.1 BT006868 mRNA Translation: AAP35514.1 AK297554 mRNA Translation: BAG59947.1 AL596094 Genomic DNA No translation available. BC005145 mRNA Translation: AAH05145.1 AF144713 mRNA Translation: AAD34588.1 |
CCDSi | CCDS44352.1 [P50395-2] CCDS7071.1 [P50395-1] |
RefSeqi | NP_001108628.1, NM_001115156.1 [P50395-2] NP_001485.2, NM_001494.3 [P50395-1] |
3D structure databases
SMRi | P50395 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 108933, 70 interactors |
DIPi | DIP-50593N |
IntActi | P50395, 45 interactors |
MINTi | P50395 |
STRINGi | 9606.ENSP00000369538 |
PTM databases
iPTMneti | P50395 |
MetOSitei | P50395 |
PhosphoSitePlusi | P50395 |
SwissPalmi | P50395 |
Polymorphism and mutation databases
BioMutai | GDI2 |
DMDMi | 13638228 |
2D gel databases
OGPi | P50395 |
REPRODUCTION-2DPAGEi | IPI00031461 P50395 |
Proteomic databases
CPTACi | CPTAC-512 CPTAC-513 |
EPDi | P50395 |
jPOSTi | P50395 |
MassIVEi | P50395 |
PaxDbi | P50395 |
PeptideAtlasi | P50395 |
PRIDEi | P50395 |
ProteomicsDBi | 56216 [P50395-1] 56217 [P50395-2] |
Protocols and materials databases
Antibodypediai | 10649, 238 antibodies |
DNASUi | 2665 |
Genome annotation databases
Ensembli | ENST00000380181; ENSP00000369528; ENSG00000057608 [P50395-2] ENST00000380191; ENSP00000369538; ENSG00000057608 [P50395-1] |
GeneIDi | 2665 |
KEGGi | hsa:2665 |
UCSCi | uc001iim.5, human [P50395-1] |
Organism-specific databases
CTDi | 2665 |
DisGeNETi | 2665 |
EuPathDBi | HostDB:ENSG00000057608.16 |
GeneCardsi | GDI2 |
HGNCi | HGNC:4227, GDI2 |
HPAi | ENSG00000057608, Low tissue specificity |
MIMi | 600767, gene |
neXtProti | NX_P50395 |
OpenTargetsi | ENSG00000057608 |
PharmGKBi | PA28642 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1439, Eukaryota |
GeneTreei | ENSGT00950000182994 |
HOGENOMi | CLU_021695_0_0_1 |
InParanoidi | P50395 |
OMAi | PAMNEEY |
PhylomeDBi | P50395 |
TreeFami | TF300449 |
Enzyme and pathway databases
PathwayCommonsi | P50395 |
Reactomei | R-HSA-194840, Rho GTPase cycle R-HSA-6798695, Neutrophil degranulation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
BioGRID-ORCSi | 2665, 42 hits in 850 CRISPR screens |
ChiTaRSi | GDI2, human |
GeneWikii | GDI2 |
GenomeRNAii | 2665 |
Pharosi | P50395, Tbio |
PROi | PR:P50395 |
RNActi | P50395, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000057608, Expressed in female gonad and 247 other tissues |
ExpressionAtlasi | P50395, baseline and differential |
Genevisiblei | P50395, HS |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR018203, GDP_dissociation_inhibitor IPR000806, RabGDI |
PANTHERi | PTHR11787, PTHR11787, 1 hit |
Pfami | View protein in Pfam PF00996, GDI, 1 hit |
PRINTSi | PR00892, RABGDI PR00891, RABGDIREP |
SUPFAMi | SSF51905, SSF51905, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GDIB_HUMAN | |
Accessioni | P50395Primary (citable) accession number: P50395 Secondary accession number(s): O43928, Q5SX88, Q9UQM6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | April 27, 2001 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM