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Protein

Phosphoglycerate kinase 2, chloroplastic

Gene

At1g56190

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113SubstrateBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei227SubstrateBy similarity1
Binding sitei278ATPBy similarity1
Binding sitei369ATP; via carbonyl oxygenBy similarity1
Binding sitei400ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi429 – 432ATPBy similarity4

GO - Molecular functioni

  • ADP binding Source: GO_Central
  • ATP binding Source: GO_Central
  • phosphoglycerate kinase activity Source: GO_Central
  • protein kinase activity Source: TAIR

GO - Biological processi

  • gluconeogenesis Source: GO_Central
  • glycolytic process Source: TAIR
  • reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  • regulation of defense response to virus by host Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: GO_Central

Keywordsi

Molecular functionKinase, Transferase
Biological processCalvin cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G56190-MONOMER
ReactomeiR-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
UniPathwayi
UPA00116

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2, chloroplastic (EC:2.7.2.3)
Gene namesi
Ordered Locus Names:At1g56190
ORF Names:F14G9.19, T6H22.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G56190
TAIRilocus:2205215 AT1G56190

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 74ChloroplastCuratedAdd BLAST74
ChainiPRO_000002388975 – 478Phosphoglycerate kinase 2, chloroplasticAdd BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP50318
PRIDEiP50318

PTM databases

iPTMnetiP50318

Expressioni

Gene expression databases

ExpressionAtlasiP50318 baseline and differential
GenevisibleiP50318 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi27297, 1 interactor
STRINGi3702.AT1G56190.1

Structurei

3D structure databases

ProteinModelPortaliP50318
SMRiP50318
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 99Substrate bindingBy similarity3
Regioni136 – 139Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1367 Eukaryota
COG0126 LUCA
HOGENOMiHOG000227107
InParanoidiP50318
KOiK00927
OrthoDBiEOG09360DE5

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 2 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P50318-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASTAATAAL SIIKSTGGAA VTRSSRASFG HIPSTSVSAR RLGFSAVVDS
60 70 80 90 100
RFSVHVASKV HSVRGKGARG VITMAKKSVG DLNSVDLKGK KVFVRADLNV
110 120 130 140 150
PLDDNQNITD DTRIRAAIPT IKFLIENGAK VILSTHLGRP KGVTPKFSLA
160 170 180 190 200
PLVPRLSELL GIEVVKADDC IGPEVETLVA SLPEGGVLLL ENVRFYKEEE
210 220 230 240 250
KNEPDFAKKL ASLADLYVND AFGTAHRAHA STEGVTKFLK PSVAGFLLQK
260 270 280 290 300
ELDYLVGAVS NPKRPFAAIV GGSKVSSKIG VIESLLEKCD ILLLGGGMIF
310 320 330 340 350
TFYKAQGLSV GSSLVEEDKL ELATTLLAKA KARGVSLLLP TDVVIADKFA
360 370 380 390 400
PDANSKIVPA SAIPDGWMGL DIGPDSVKTF NEALDTTQTV IWNGPMGVFE
410 420 430 440 450
FEKFAKGTEA VANKLAELSK KGVTTIIGGG DSVAAVEKVG VAGVMSHIST
460 470
GGGASLELLE GKVLPGVVAL DEATPVTV
Length:478
Mass (Da):49,939
Last modified:May 3, 2011 - v3
Checksum:i03751560F052E6F8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F4I3L1F4I3L1_ARATH
Phosphoglycerate kinase
At1g56190, F14G9.19, F14G9_19
405Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti412A → R in AAA79705 (PubMed:10520454).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009894 Genomic DNA Translation: AAF02830.1
AC069159 Genomic DNA Translation: AAG50920.1
CP002684 Genomic DNA Translation: AEE33356.1
AY056291 mRNA Translation: AAL07140.1
AY099598 mRNA Translation: AAM20449.1
BT000250 mRNA Translation: AAN15569.1
U37700 mRNA Translation: AAA79705.1
PIRiD96603
S71214
RefSeqiNP_176015.1, NM_104498.4 [P50318-1]
UniGeneiAt.11481

Genome annotation databases

EnsemblPlantsiAT1G56190.1; AT1G56190.1; AT1G56190 [P50318-1]
GeneIDi842072
GrameneiAT1G56190.1; AT1G56190.1; AT1G56190 [P50318-1]
KEGGiath:AT1G56190

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009894 Genomic DNA Translation: AAF02830.1
AC069159 Genomic DNA Translation: AAG50920.1
CP002684 Genomic DNA Translation: AEE33356.1
AY056291 mRNA Translation: AAL07140.1
AY099598 mRNA Translation: AAM20449.1
BT000250 mRNA Translation: AAN15569.1
U37700 mRNA Translation: AAA79705.1
PIRiD96603
S71214
RefSeqiNP_176015.1, NM_104498.4 [P50318-1]
UniGeneiAt.11481

3D structure databases

ProteinModelPortaliP50318
SMRiP50318
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27297, 1 interactor
STRINGi3702.AT1G56190.1

PTM databases

iPTMnetiP50318

Proteomic databases

PaxDbiP50318
PRIDEiP50318

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G56190.1; AT1G56190.1; AT1G56190 [P50318-1]
GeneIDi842072
GrameneiAT1G56190.1; AT1G56190.1; AT1G56190 [P50318-1]
KEGGiath:AT1G56190

Organism-specific databases

AraportiAT1G56190
TAIRilocus:2205215 AT1G56190

Phylogenomic databases

eggNOGiKOG1367 Eukaryota
COG0126 LUCA
HOGENOMiHOG000227107
InParanoidiP50318
KOiK00927
OrthoDBiEOG09360DE5

Enzyme and pathway databases

UniPathwayi
UPA00116

BioCyciARA:AT1G56190-MONOMER
ReactomeiR-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis

Miscellaneous databases

PROiPR:P50318

Gene expression databases

ExpressionAtlasiP50318 baseline and differential
GenevisibleiP50318 AT

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 2 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGKH2_ARATH
AccessioniPrimary (citable) accession number: P50318
Secondary accession number(s): Q42542, Q9C7J4, Q9SGU6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 3, 2011
Last modified: November 7, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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