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Protein

Isoaspartyl peptidase/L-asparaginase 1

Gene

At5g08100

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts in asparagine catabolism but also in the final steps of protein and degradation via hydrolysis of a range of isoaspartyl dipeptides. The affinity for Asn and at least 4 isoaspartyl dipeptides (L-beta-Asp-Ala, L-beta-Asp-Gly, L-beta-Asp-Leu, L-beta-Asp-Phe) is quite low, KM being greater than 4.0 mM. The enzyme is inactive on alpha-aspartyl dipeptides.1 Publication

Catalytic activityi

Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei183Nucleophile1

GO - Molecular functioni

  • asparaginase activity Source: TAIR
  • beta-aspartyl-peptidase activity Source: UniProtKB-EC

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT5G08100-MONOMER
SABIO-RKiP50287

Protein family/group databases

MEROPSiT02.A01

Names & Taxonomyi

Protein namesi
Recommended name:
Isoaspartyl peptidase/L-asparaginase 1 (EC:3.4.19.5)
Alternative name(s):
L-asparagine amidohydrolase 1
Cleaved into the following 2 chains:
Gene namesi
Ordered Locus Names:At5g08100
ORF Names:T22D6_40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G08100
TAIRilocus:2181509 AT5G08100

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000454421 – 182Isoaspartyl peptidase/L-asparaginase 1 subunit alphaAdd BLAST182
ChainiPRO_0000045443183 – 315Isoaspartyl peptidase/L-asparaginase 1 subunit betaAdd BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169PhosphoserineCombined sources1

Post-translational modificationi

Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei182 – 183Cleavage; by autolysis2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

PaxDbiP50287
PRIDEiP50287

PTM databases

iPTMnetiP50287

Expressioni

Gene expression databases

ExpressionAtlasiP50287 baseline and differential
GenevisibleiP50287 AT

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G08100.1

Structurei

3D structure databases

ProteinModelPortaliP50287
SMRiP50287
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 214Substrate bindingBy similarity4
Regioni233 – 236Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the Ntn-hydrolase family.Curated

Phylogenomic databases

eggNOGiKOG1592 Eukaryota
COG1446 LUCA
HOGENOMiHOG000174613
InParanoidiP50287
KOiK13051
OMAiGITGKWP
OrthoDBiEOG09360LPG
PhylomeDBiP50287

Family and domain databases

InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000246 Peptidase_T2
PANTHERiPTHR10188 PTHR10188, 1 hit
PfamiView protein in Pfam
PF01112 Asparaginase_2, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P50287-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGWAIALHG GAGDIPIDLP DERRIPRESA LRHCLDLGIS ALKSGKPPLD
60 70 80 90 100
VAELVVRELE NHPDFNAGKG SVLTAQGTVE MEASIMDGKT KRCGAVSGLT
110 120 130 140 150
TVVNPISLAR LVMEKTPHIY LAFDAAEAFA RAHGVETVDS SHFITPENIA
160 170 180 190 200
RLKQAKEFNR VQLDYTVPSP KVPDNCGDSQ IGTVGCVAVD SAGNLASATS
210 220 230 240 250
TGGYVNKMVG RIGDTPVIGA GTYANHLCAI SATGKGEDII RGTVARDVAA
260 270 280 290 300
LMEYKGLSLT EAAAYVVDQS VPRGSCGLVA VSANGEVTMP FNTTGMFRAC
310
ASEDGYSEIA IWPNN
Length:315
Mass (Da):33,027
Last modified:November 15, 2002 - v2
Checksum:iB6D3202B42F94712
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139D → Y in CAA84367 (PubMed:7630960).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34884 Genomic DNA Translation: CAA84367.1
AL357612 Genomic DNA Translation: CAB93711.1
CP002688 Genomic DNA Translation: AED91247.1
AY039555 mRNA Translation: AAK62610.1
AY093755 mRNA Translation: AAM10379.1
PIRiS53127
T50495
RefSeqiNP_196427.1, NM_120892.4 [P50287-1]
UniGeneiAt.5449
At.54776

Genome annotation databases

EnsemblPlantsiAT5G08100.1; AT5G08100.1; AT5G08100 [P50287-1]
GeneIDi830704
GrameneiAT5G08100.1; AT5G08100.1; AT5G08100 [P50287-1]
KEGGiath:AT5G08100

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASPGA_ARATH
AccessioniPrimary (citable) accession number: P50287
Secondary accession number(s): Q9LEZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 15, 2002
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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