We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P50277 (BIOA_YEAST)
Protein
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Gene
BIO3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA) (PubMed:10333520).
It is the only aminotransferase known to utilize SAM as an amino donor (PubMed:10333520).
1 PublicationCatalytic activityi
- (S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate1 PublicationEC:2.6.1.621 Publication
Cofactori
pyridoxal 5'-phosphateBy similarity
: biotin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).1 Publication This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 160 | SubstrateBy similarity | 1 | |
Binding sitei | 270 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 350 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 441 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: SGD
- dethiobiotin synthase activity Source: GO_Central
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- biotin biosynthetic process Source: SGD
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Biotin biosynthesis |
Ligand | Pyridoxal phosphate, S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00078;UER00160 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosylmethionine-8-amino-7-oxononanoate aminotransferaseCurated (EC:2.6.1.621 Publication)Alternative name(s): 7,8-diamino-pelargonic acid aminotransferase1 Publication Short name: DAPA AT1 Publication Short name: DAPA aminotransferase1 Publication Diaminopelargonic acid synthaseCurated |
Gene namesi | Name:BIO31 Publication Ordered Locus Names:YNR058W ORF Names:N3510 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005341, BIO3 |
VEuPathDBi | FungiDB:YNR058W |
Subcellular locationi
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120378 | 1 – 480 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferaseAdd BLAST | 480 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 314 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
PaxDbi | P50277 |
PTM databases
iPTMneti | P50277 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35884, 23 interactors |
DIPi | DIP-4822N |
IntActi | P50277, 5 interactors |
MINTi | P50277 |
STRINGi | 4932.YNR058W |
Miscellaneous databases
RNActi | P50277, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 126 – 127 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 351 – 352 | Pyridoxal phosphate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1401, Eukaryota |
GeneTreei | ENSGT00940000176655 |
HOGENOMi | CLU_016922_4_3_1 |
InParanoidi | P50277 |
OMAi | VAVKMCL |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00834, BioA, 1 hit |
InterProi | View protein in InterPro IPR005814, Aminotrans_3 IPR005815, BioA IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00508, bioA, 1 hit |
i Sequence
Sequence statusi: Complete.
P50277-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQEISYTPD VAELLDFDKK HIWHPYTSLS SPLNVYPVKS AHGCKLVLDT
60 70 80 90 100
DSPVDVEVID AMSSWWCVIH GYNNPELNEA LTKQMLKFSH VLLGGFTHKG
110 120 130 140 150
AVNLVQKLLK VIDEPSLQYC FLADSGSVAV EVALKMALQS NMSGEATKNR
160 170 180 190 200
TKFLTIKNGY HGDTFGAMSV CDPENSMHHI YNDRLSENIF AQAPSIVDGL
210 220 230 240 250
PTSQNGFEDH WNAEEVTDLK KQFELHSDKI CAVILEPILQ GAGGLRPYHP
260 270 280 290 300
QFLIEVQKLC NQYDVLFIMD EIATGFGRTG EIFAFKHCQK YQDQHGISPS
310 320 330 340 350
DQIKVVPDIL CVGKGLTSGY MTMSAVVVND KVASRISSPN SPTGGCFMHG
360 370 380 390 400
PTFMGNALAC SVAEKSMDIL LRGEWRKQVS AIENQIYREL YQYIKNPDNG
410 420 430 440 450
LIGTVVKRVS VIGAVGIVEL YKKTDPEWFQ KKFISKGVHI RPFNCLCYIM
460 470 480
PPYVITTEEL TKVNQVLIEV LHEWKSHINQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 140 | S → P in AAU09779 (PubMed:17322287).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 229 | K → E in strain: ATCC 28383 and ATCC 204626. 1 Publication | 1 | |
Natural varianti | 295 | H → R in strain: ATCC 28383 and ATCC 204626. 1 Publication | 1 | |
Natural varianti | 384 | N → D in strain: ATCC 28383 and ATCC 204626. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U47112 Genomic DNA Translation: AAA88905.1 U53467 Genomic DNA Translation: AAB63970.1 AB200248 Genomic DNA Translation: BAE00005.1 Z71673 Genomic DNA Translation: CAA96340.1 AY723862 Genomic DNA Translation: AAU09779.1 BK006947 Genomic DNA Translation: DAA10599.1 |
PIRi | S63390 |
RefSeqi | NP_014456.1, NM_001183235.1 |
Genome annotation databases
EnsemblFungii | YNR058W_mRNA; YNR058W; YNR058W |
GeneIDi | 855795 |
KEGGi | sce:YNR058W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U47112 Genomic DNA Translation: AAA88905.1 U53467 Genomic DNA Translation: AAB63970.1 AB200248 Genomic DNA Translation: BAE00005.1 Z71673 Genomic DNA Translation: CAA96340.1 AY723862 Genomic DNA Translation: AAU09779.1 BK006947 Genomic DNA Translation: DAA10599.1 |
PIRi | S63390 |
RefSeqi | NP_014456.1, NM_001183235.1 |
3D structure databases
AlphaFoldDBi | P50277 |
SMRi | P50277 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35884, 23 interactors |
DIPi | DIP-4822N |
IntActi | P50277, 5 interactors |
MINTi | P50277 |
STRINGi | 4932.YNR058W |
PTM databases
iPTMneti | P50277 |
Proteomic databases
PaxDbi | P50277 |
Genome annotation databases
EnsemblFungii | YNR058W_mRNA; YNR058W; YNR058W |
GeneIDi | 855795 |
KEGGi | sce:YNR058W |
Organism-specific databases
SGDi | S000005341, BIO3 |
VEuPathDBi | FungiDB:YNR058W |
Phylogenomic databases
eggNOGi | KOG1401, Eukaryota |
GeneTreei | ENSGT00940000176655 |
HOGENOMi | CLU_016922_4_3_1 |
InParanoidi | P50277 |
OMAi | VAVKMCL |
Enzyme and pathway databases
UniPathwayi | UPA00078;UER00160 |
Miscellaneous databases
PROi | PR:P50277 |
RNActi | P50277, protein |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00834, BioA, 1 hit |
InterProi | View protein in InterPro IPR005814, Aminotrans_3 IPR005815, BioA IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00508, bioA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BIOA_YEAST | |
Accessioni | P50277Primary (citable) accession number: P50277 Secondary accession number(s): D6W1N3, E9P967, Q4R1J0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families