UniProtKB - P50225 (ST1A1_HUMAN)
Protein
Sulfotransferase 1A1
Gene
SULT1A1
Organism
Homo sapiens (Human)
Status
Functioni
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of a wide variety of acceptor molecules bearing a hydroxyl or an amine groupe. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Displays broad substrate specificity for small phenolic compounds. Plays an important role in the sulfonation of endogenous molecules such as steroid hormones and 3,3'-diiodothyronin (PubMed:16221673, PubMed:12471039, PubMed:22069470, PubMed:21723874, PubMed:10199779, PubMed:7834621). Mediates the sulfate conjugation of a variety of xenobiotics, including the drugs acetaminophen and minoxidil (By similarity). Mediates also the metabolic activation of carcinogenic N-hydroxyarylamines leading to highly reactive intermediates capable of forming DNA adducts, potentially resulting in mutagenesis (PubMed:7834621).By similarity6 Publications
Catalytic activityi
- 3'-phosphoadenylyl sulfate + a phenol = a phenyl sulfate + adenosine 3',5'-bisphosphate + H+6 PublicationsEC:2.8.2.16 PublicationsThis reaction proceeds in the forward1 Publication direction.
- 17β-estradiol + 3'-phosphoadenylyl sulfate = 17β-estradiol 3-sulfate + adenosine 3',5'-bisphosphate + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=0.12 µM for 3,3'-diiodothyronin1 Publication
- Vmax=465 µmol/min/mg enzyme with 3,3'-diiodothyronin as substrate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 108 | Proton acceptorBy similarity | 1 | |
Binding sitei | 130 | PAPS2 Publications | 1 | |
Binding sitei | 138 | PAPS2 Publications | 1 | |
Binding sitei | 193 | PAPS2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 48 – 53 | PAPS2 Publications | 6 | |
Nucleotide bindingi | 227 – 232 | PAPS2 Publications | 6 | |
Nucleotide bindingi | 255 – 259 | PAPS2 Publications | 5 |
GO - Molecular functioni
- 3'-phosphoadenosine 5'-phosphosulfate binding Source: UniProtKB
- aryl sulfotransferase activity Source: UniProtKB
- flavonol 3-sulfotransferase activity Source: BHF-UCL
- steroid sulfotransferase activity Source: UniProtKB
- sulfotransferase activity Source: MGI
GO - Biological processi
- 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: UniProtKB
- amine metabolic process Source: ProtInc
- catecholamine metabolic process Source: UniProtKB-KW
- estrogen metabolic process Source: UniProtKB
- ethanol catabolic process Source: CAFA
- flavonoid metabolic process Source: BHF-UCL
- sulfation Source: BHF-UCL
- xenobiotic metabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Catecholamine metabolism, Lipid metabolism, Steroid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:HS09898-MONOMER |
BRENDAi | 2.8.2.1, 2681 |
PathwayCommonsi | P50225 |
Reactomei | R-HSA-156584, Cytosolic sulfonation of small molecules |
SABIO-RKi | P50225 |
Chemistry databases
SwissLipidsi | SLP:000001651 |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfotransferase 1A1 (EC:2.8.2.16 Publications)Short name: ST1A1 Alternative name(s): Aryl sulfotransferase 11 Publication HAST1/HAST2 Phenol sulfotransferase 1 Phenol-sulfating phenol sulfotransferase 11 Publication Short name: P-PST 11 Publication ST1A3 Thermostable phenol sulfotransferase Short name: Ts-PST |
Gene namesi | Name:SULT1A1 Synonyms:STP, STP1 ORF Names:OK/SW-cl.88 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11453, SULT1A1 |
MIMi | 171150, gene |
neXtProti | NX_P50225 |
VEuPathDBi | HostDB:ENSG00000196502.11 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 70 | C → S: Increased sensitivity of enzyme activity to heat inactivation. 1 Publication | 1 | |
Mutagenesisi | 249 | D → G: Increased activity towards p-nitrophenol. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6817 |
OpenTargetsi | ENSG00000196502 |
PharmGKBi | PA343 |
Miscellaneous databases
Pharosi | P50225, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1743291 |
DrugBanki | DB00316, Acetaminophen DB01812, Adenosine 3',5'-diphosphate DB00714, Apomorphine DB04417, P-Nitrophenol DB11077, Polyethylene glycol 400 DB09288, Propacetamol DB00675, Tamoxifen DB09100, Thyroid, porcine DB09070, Tibolone |
Genetic variation databases
BioMutai | SULT1A1 |
DMDMi | 313104286 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085127 | 1 – 295 | Sulfotransferase 1A1Add BLAST | 295 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 138 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P50225 |
jPOSTi | P50225 |
MassIVEi | P50225 |
MaxQBi | P50225 |
PaxDbi | P50225 |
PeptideAtlasi | P50225 |
PRIDEi | P50225 |
ProteomicsDBi | 56209 [P50225-1] 56210 [P50225-2] |
2D gel databases
OGPi | P50225 |
PTM databases
iPTMneti | P50225 |
PhosphoSitePlusi | P50225 |
Expressioni
Tissue specificityi
Liver, lung, adrenal, brain, platelets and skin.
Gene expression databases
Bgeei | ENSG00000196502, Expressed in right adrenal gland and 154 other tissues |
ExpressionAtlasi | P50225, baseline and differential |
Genevisiblei | P50225, HS |
Organism-specific databases
HPAi | ENSG00000196502, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsBinary interactionsi
P50225
With | #Exp. | IntAct |
---|---|---|
SULT2B1 [O00204] | 7 | EBI-2814403,EBI-749441 |
Protein-protein interaction databases
BioGRIDi | 112686, 45 interactors |
IntActi | P50225, 10 interactors |
STRINGi | 9606.ENSP00000378972 |
Chemistry databases
BindingDBi | P50225 |
Miscellaneous databases
RNActi | P50225, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P50225 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P50225 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 108 | Substrate binding2 Publications | 3 |
Sequence similaritiesi
Belongs to the sulfotransferase 1 family.Curated
Phylogenomic databases
eggNOGi | KOG1584, Eukaryota |
GeneTreei | ENSGT00940000163450 |
HOGENOMi | CLU_027239_6_1_1 |
InParanoidi | P50225 |
OMAi | TDLQFRT |
OrthoDBi | 780670at2759 |
PhylomeDBi | P50225 |
TreeFami | TF321745 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P50225-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELIQDTSRP PLEYVKGVPL IKYFAEALGP LQSFQARPDD LLISTYPKSG
60 70 80 90 100
TTWVSQILDM IYQGGDLEKC HRAPIFMRVP FLEFKAPGIP SGMETLKDTP
110 120 130 140 150
APRLLKTHLP LALLPQTLLD QKVKVVYVAR NAKDVAVSYY HFYHMAKVHP
160 170 180 190 200
EPGTWDSFLE KFMVGEVSYG SWYQHVQEWW ELSRTHPVLY LFYEDMKENP
210 220 230 240 250
KREIQKILEF VGRSLPEETV DFVVQHTSFK EMKKNPMTNY TTVPQEFMDH
260 270 280 290
SISPFMRKGM AGDWKTTFTV AQNERFDADY AEKMAGCSLS FRSEL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BRY5 | H3BRY5_HUMAN | Sulfotransferase | SULT1A1 | 281 | Annotation score: | ||
H3BQX5 | H3BQX5_HUMAN | Sulfotransferase | SULT1A1 | 169 | Annotation score: | ||
H3BT96 | H3BT96_HUMAN | Sulfotransferase 1A1 | SULT1A1 | 85 | Annotation score: | ||
H3BMH9 | H3BMH9_HUMAN | Sulfotransferase 1A1 | SULT1A1 | 56 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 90 | P → L in AAA67895 (PubMed:8423770).Curated | 1 | |
Sequence conflicti | 146 | A → T in CAA59147 (PubMed:7864863).Curated | 1 | |
Sequence conflicti | 181 | E → G in CAA59147 (PubMed:7864863).Curated | 1 | |
Sequence conflicti | 243 | V → A in AAA67895 (PubMed:8423770).Curated | 1 | |
Sequence conflicti | 290 | S → T in AAA99892 (PubMed:8423770).Curated | 1 | |
Sequence conflicti | 290 | S → T in AAC50480 (PubMed:8924211).Curated | 1 |
Polymorphismi
There are several alleles. The sequence shown is that of allele SULT1A1*3.2 Publications
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_009302 | 37 | R → Q1 PublicationCorresponds to variant dbSNP:rs72547527Ensembl. | 1 | |
Natural variantiVAR_028721 | 151 | E → D. Corresponds to variant dbSNP:rs1042014Ensembl. | 1 | |
Natural variantiVAR_057339 | 151 | E → Q. Corresponds to variant dbSNP:rs1042011Ensembl. | 1 | |
Natural variantiVAR_007425 | 213 | R → H in allele SULT1A1*2; has a lower sulfotransferase activity. 4 PublicationsCorresponds to variant dbSNP:rs1042028Ensembl. | 1 | |
Natural variantiVAR_009303 | 223 | V → MCombined sources14 PublicationsCorresponds to variant dbSNP:rs1801030Ensembl. | 1 | |
Natural variantiVAR_014889 | 235 | N → T. Corresponds to variant dbSNP:rs35728980Ensembl. | 1 | |
Natural variantiVAR_061886 | 282 | E → K2 PublicationsCorresponds to variant dbSNP:rs36043491Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040101 | 1 – 124 | MELIQ…DQKVK → MLAKLLCDQVVGAPIAVSAF YAGMSILQGKDDIFLDLKQK FWNTYM in isoform 2. 1 PublicationAdd BLAST | 124 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000314752; ENSP00000321988; ENSG00000196502 [P50225-1] ENST00000350842; ENSP00000329399; ENSG00000196502 [P50225-2] ENST00000395607; ENSP00000378971; ENSG00000196502 [P50225-1] ENST00000395609; ENSP00000378972; ENSG00000196502 [P50225-1] ENST00000569554; ENSP00000457912; ENSG00000196502 [P50225-1] |
GeneIDi | 6817 |
KEGGi | hsa:6817 |
UCSCi | uc002dqi.4, human [P50225-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LS6 | X-ray | 1.90 | A | 1-295 | [»] | |
1Z28 | X-ray | 2.30 | A | 1-295 | [»] | |
2D06 | X-ray | 2.30 | A/B | 1-295 | [»] | |
3QVU | X-ray | 2.50 | A/B | 1-295 | [»] | |
3QVV | X-ray | 2.35 | A/B | 1-295 | [»] | |
3U3J | X-ray | 2.70 | A/B | 1-294 | [»] | |
3U3K | X-ray | 2.36 | A/B | 1-295 | [»] | |
3U3M | X-ray | 2.30 | A | 1-295 | [»] | |
3U3O | X-ray | 2.00 | A | 1-295 | [»] | |
3U3R | X-ray | 2.36 | A | 1-295 | [»] | |
4GRA | X-ray | 2.56 | A/B | 1-295 | [»] | |
SMRi | P50225 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112686, 45 interactors |
IntActi | P50225, 10 interactors |
STRINGi | 9606.ENSP00000378972 |
Chemistry databases
BindingDBi | P50225 |
ChEMBLi | CHEMBL1743291 |
DrugBanki | DB00316, Acetaminophen DB01812, Adenosine 3',5'-diphosphate DB00714, Apomorphine DB04417, P-Nitrophenol DB11077, Polyethylene glycol 400 DB09288, Propacetamol DB00675, Tamoxifen DB09100, Thyroid, porcine DB09070, Tibolone |
SwissLipidsi | SLP:000001651 |
PTM databases
iPTMneti | P50225 |
PhosphoSitePlusi | P50225 |
Genetic variation databases
BioMutai | SULT1A1 |
DMDMi | 313104286 |
2D gel databases
OGPi | P50225 |
Proteomic databases
EPDi | P50225 |
jPOSTi | P50225 |
MassIVEi | P50225 |
MaxQBi | P50225 |
PaxDbi | P50225 |
PeptideAtlasi | P50225 |
PRIDEi | P50225 |
ProteomicsDBi | 56209 [P50225-1] 56210 [P50225-2] |
Protocols and materials databases
Antibodypediai | 26492, 446 antibodies |
DNASUi | 6817 |
Genome annotation databases
Ensembli | ENST00000314752; ENSP00000321988; ENSG00000196502 [P50225-1] ENST00000350842; ENSP00000329399; ENSG00000196502 [P50225-2] ENST00000395607; ENSP00000378971; ENSG00000196502 [P50225-1] ENST00000395609; ENSP00000378972; ENSG00000196502 [P50225-1] ENST00000569554; ENSP00000457912; ENSG00000196502 [P50225-1] |
GeneIDi | 6817 |
KEGGi | hsa:6817 |
UCSCi | uc002dqi.4, human [P50225-1] |
Organism-specific databases
CTDi | 6817 |
DisGeNETi | 6817 |
GeneCardsi | SULT1A1 |
HGNCi | HGNC:11453, SULT1A1 |
HPAi | ENSG00000196502, Tissue enhanced (liver) |
MIMi | 171150, gene |
neXtProti | NX_P50225 |
OpenTargetsi | ENSG00000196502 |
PharmGKBi | PA343 |
VEuPathDBi | HostDB:ENSG00000196502.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1584, Eukaryota |
GeneTreei | ENSGT00940000163450 |
HOGENOMi | CLU_027239_6_1_1 |
InParanoidi | P50225 |
OMAi | TDLQFRT |
OrthoDBi | 780670at2759 |
PhylomeDBi | P50225 |
TreeFami | TF321745 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS09898-MONOMER |
BRENDAi | 2.8.2.1, 2681 |
PathwayCommonsi | P50225 |
Reactomei | R-HSA-156584, Cytosolic sulfonation of small molecules |
SABIO-RKi | P50225 |
Miscellaneous databases
BioGRID-ORCSi | 6817, 6 hits in 851 CRISPR screens |
ChiTaRSi | SULT1A1, human |
EvolutionaryTracei | P50225 |
GeneWikii | SULT1A1 |
GenomeRNAii | 6817 |
Pharosi | P50225, Tbio |
PROi | PR:P50225 |
RNActi | P50225, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196502, Expressed in right adrenal gland and 154 other tissues |
ExpressionAtlasi | P50225, baseline and differential |
Genevisiblei | P50225, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ST1A1_HUMAN | |
Accessioni | P50225Primary (citable) accession number: P50225 Secondary accession number(s): Q2NL71 Q9UGG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 10, 2021 | |
This is version 194 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families