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UniProtKB - P50150 (GBG4_HUMAN)
Protein
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
Gene
GNG4
Organism
Homo sapiens (Human)
Status
Functioni
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
CuratedGO - Molecular functioni
- G-protein beta-subunit binding Source: GO_Central
GO - Biological processi
- G protein-coupled receptor signaling pathway Source: GO_Central
- negative regulation of cell growth Source: BHF-UCL
- regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Keywordsi
Molecular function | Transducer |
Enzyme and pathway databases
PathwayCommonsi | P50150 |
Reactomei | R-HSA-1296041, Activation of G protein gated Potassium channels R-HSA-163359, Glucagon signaling in metabolic regulation R-HSA-202040, G-protein activation R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-392170, ADP signalling through P2Y purinoceptor 12 R-HSA-392451, G beta:gamma signalling through PI3Kgamma R-HSA-392851, Prostacyclin signalling through prostacyclin receptor R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-4086398, Ca2+ pathway R-HSA-416476, G alpha (q) signalling events R-HSA-416482, G alpha (12/13) signalling events R-HSA-418217, G beta:gamma signalling through PLC beta R-HSA-418555, G alpha (s) signalling events R-HSA-418592, ADP signalling through P2Y purinoceptor 1 R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events R-HSA-420092, Glucagon-type ligand receptors R-HSA-428930, Thromboxane signalling through TP receptor R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-456926, Thrombin signalling through proteinase activated receptors (PARs) R-HSA-500657, Presynaptic function of Kainate receptors R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-8964315, G beta:gamma signalling through BTK R-HSA-8964616, G beta:gamma signalling through CDC42 R-HSA-9009391, Extra-nuclear estrogen signaling R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
SignaLinki | P50150 |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 |
Gene namesi | Name:GNG4 Synonyms:GNGT4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4407, GNG4 |
MIMi | 604388, gene |
neXtProti | NX_P50150 |
VEuPathDBi | HostDB:ENSG00000168243 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: Reactome
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 2786 |
OpenTargetsi | ENSG00000168243 |
PharmGKBi | PA28786 |
Miscellaneous databases
Pharosi | P50150, Tbio |
Genetic variation databases
BioMutai | GNG4 |
DMDMi | 1730219 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000012621 | 1 – 72 | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4Add BLAST | 72 | |
PropeptideiPRO_0000012622 | 73 – 75 | Removed in mature form | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 72 | S-geranylgeranyl cysteine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
EPDi | P50150 |
jPOSTi | P50150 |
MassIVEi | P50150 |
MaxQBi | P50150 |
PaxDbi | P50150 |
PeptideAtlasi | P50150 |
PRIDEi | P50150 |
ProteomicsDBi | 56199 |
PTM databases
iPTMneti | P50150 |
PhosphoSitePlusi | P50150 |
Expressioni
Tissue specificityi
Brain, kidney, pancreas, skeletal muscle and faintly in cardiac muscle.
Gene expression databases
Bgeei | ENSG00000168243, Expressed in subventricular zone (outer) (primate) and 148 other tissues |
ExpressionAtlasi | P50150, baseline and differential |
Genevisiblei | P50150, HS |
Organism-specific databases
HPAi | ENSG00000168243, Tissue enhanced (brain, ovary, pituitary gland) |
Interactioni
Subunit structurei
G proteins are composed of 3 units, alpha, beta and gamma.
Interacts with beta-1 and beta-2, but not with beta-3 (PubMed:7665596).
Interacts with KCNK1 (By similarity).
By similarity1 PublicationBinary interactionsi
P50150
With | #Exp. | IntAct |
---|---|---|
FHL2 [Q14192] | 3 | EBI-6395970,EBI-701903 |
Kcnk2 [Q920B6] from Rattus norvegicus. | 3 | EBI-6395970,EBI-6530063 |
GO - Molecular functioni
- G-protein beta-subunit binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 109048, 26 interactors |
CORUMi | P50150 |
IntActi | P50150, 15 interactors |
STRINGi | 9606.ENSP00000375727 |
Miscellaneous databases
RNActi | P50150, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G protein gamma family.Curated
Phylogenomic databases
eggNOGi | KOG4119, Eukaryota |
GeneTreei | ENSGT00990000203647 |
HOGENOMi | CLU_168377_0_1_1 |
InParanoidi | P50150 |
OMAi | HMGEDPL |
OrthoDBi | 1581168at2759 |
PhylomeDBi | P50150 |
TreeFami | TF319909 |
Family and domain databases
CDDi | cd00068, GGL, 1 hit |
Gene3Di | 4.10.260.10, 1 hit |
InterProi | View protein in InterPro IPR015898, G-protein_gamma-like_dom IPR036284, GGL_sf IPR001770, Gprotein-gamma |
PANTHERi | PTHR13809, PTHR13809, 1 hit |
Pfami | View protein in Pfam PF00631, G-gamma, 1 hit |
PRINTSi | PR00321, GPROTEING |
SMARTi | View protein in SMART SM00224, GGL, 1 hit |
SUPFAMi | SSF48670, SSF48670, 1 hit |
PROSITEi | View protein in PROSITE PS50058, G_PROTEIN_GAMMA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P50150-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKEGMSNNST TSISQARKAV EQLKMEACMD RVKVSQAAAD LLAYCEAHVR
60 70
EDPLIIPVPA SENPFREKKF FCTIL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U31382 mRNA Translation: AAC50204.1 AF493872 mRNA Translation: AAM12586.1 BC022485 mRNA Translation: AAH22485.1 |
CCDSi | CCDS1607.1 |
PIRi | I39157 |
RefSeqi | NP_001092191.1, NM_001098721.1 NP_001092192.1, NM_001098722.1 NP_004476.1, NM_004485.3 XP_006711824.1, XM_006711761.2 XP_011542469.1, XM_011544167.2 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U31382 mRNA Translation: AAC50204.1 AF493872 mRNA Translation: AAM12586.1 BC022485 mRNA Translation: AAH22485.1 |
CCDSi | CCDS1607.1 |
PIRi | I39157 |
RefSeqi | NP_001092191.1, NM_001098721.1 NP_001092192.1, NM_001098722.1 NP_004476.1, NM_004485.3 XP_006711824.1, XM_006711761.2 XP_011542469.1, XM_011544167.2 |
3D structure databases
AlphaFoldDBi | P50150 |
SMRi | P50150 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 109048, 26 interactors |
CORUMi | P50150 |
IntActi | P50150, 15 interactors |
STRINGi | 9606.ENSP00000375727 |
PTM databases
iPTMneti | P50150 |
PhosphoSitePlusi | P50150 |
Genetic variation databases
BioMutai | GNG4 |
DMDMi | 1730219 |
Proteomic databases
EPDi | P50150 |
jPOSTi | P50150 |
MassIVEi | P50150 |
MaxQBi | P50150 |
PaxDbi | P50150 |
PeptideAtlasi | P50150 |
PRIDEi | P50150 |
ProteomicsDBi | 56199 |
Protocols and materials databases
Antibodypediai | 34696, 94 antibodies from 23 providers |
DNASUi | 2786 |
Genome annotation databases
Organism-specific databases
CTDi | 2786 |
DisGeNETi | 2786 |
GeneCardsi | GNG4 |
HGNCi | HGNC:4407, GNG4 |
HPAi | ENSG00000168243, Tissue enhanced (brain, ovary, pituitary gland) |
MIMi | 604388, gene |
neXtProti | NX_P50150 |
OpenTargetsi | ENSG00000168243 |
PharmGKBi | PA28786 |
VEuPathDBi | HostDB:ENSG00000168243 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4119, Eukaryota |
GeneTreei | ENSGT00990000203647 |
HOGENOMi | CLU_168377_0_1_1 |
InParanoidi | P50150 |
OMAi | HMGEDPL |
OrthoDBi | 1581168at2759 |
PhylomeDBi | P50150 |
TreeFami | TF319909 |
Enzyme and pathway databases
PathwayCommonsi | P50150 |
Reactomei | R-HSA-1296041, Activation of G protein gated Potassium channels R-HSA-163359, Glucagon signaling in metabolic regulation R-HSA-202040, G-protein activation R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-392170, ADP signalling through P2Y purinoceptor 12 R-HSA-392451, G beta:gamma signalling through PI3Kgamma R-HSA-392851, Prostacyclin signalling through prostacyclin receptor R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-4086398, Ca2+ pathway R-HSA-416476, G alpha (q) signalling events R-HSA-416482, G alpha (12/13) signalling events R-HSA-418217, G beta:gamma signalling through PLC beta R-HSA-418555, G alpha (s) signalling events R-HSA-418592, ADP signalling through P2Y purinoceptor 1 R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events R-HSA-420092, Glucagon-type ligand receptors R-HSA-428930, Thromboxane signalling through TP receptor R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-456926, Thrombin signalling through proteinase activated receptors (PARs) R-HSA-500657, Presynaptic function of Kainate receptors R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-8964315, G beta:gamma signalling through BTK R-HSA-8964616, G beta:gamma signalling through CDC42 R-HSA-9009391, Extra-nuclear estrogen signaling R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
SignaLinki | P50150 |
Miscellaneous databases
BioGRID-ORCSi | 2786, 13 hits in 1068 CRISPR screens |
ChiTaRSi | GNG4, human |
GenomeRNAii | 2786 |
Pharosi | P50150, Tbio |
PROi | PR:P50150 |
RNActi | P50150, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000168243, Expressed in subventricular zone (outer) (primate) and 148 other tissues |
ExpressionAtlasi | P50150, baseline and differential |
Genevisiblei | P50150, HS |
Family and domain databases
CDDi | cd00068, GGL, 1 hit |
Gene3Di | 4.10.260.10, 1 hit |
InterProi | View protein in InterPro IPR015898, G-protein_gamma-like_dom IPR036284, GGL_sf IPR001770, Gprotein-gamma |
PANTHERi | PTHR13809, PTHR13809, 1 hit |
Pfami | View protein in Pfam PF00631, G-gamma, 1 hit |
PRINTSi | PR00321, GPROTEING |
SMARTi | View protein in SMART SM00224, GGL, 1 hit |
SUPFAMi | SSF48670, SSF48670, 1 hit |
PROSITEi | View protein in PROSITE PS50058, G_PROTEIN_GAMMA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GBG4_HUMAN | |
Accessioni | P50150Primary (citable) accession number: P50150 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families