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Protein

Guanine nucleotide-binding protein G(t) subunit alpha-2

Gene

Gnat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumBy similarity1
Metal bindingi181MagnesiumBy similarity1
Binding sitei326GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47GTPBy similarity8
Nucleotide bindingi175 – 181GTPBy similarity7
Nucleotide bindingi200 – 204GTPBy similarity5
Nucleotide bindingi269 – 272GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
Biological processSensory transduction, Vision
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-4086398 Ca2+ pathway
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-2
Alternative name(s):
Transducin alpha-2 chain
Gene namesi
Name:Gnat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95779 Gnat2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002037412 – 354Guanine nucleotide-binding protein G(t) subunit alpha-2Add BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP50149
MaxQBiP50149
PaxDbiP50149
PRIDEiP50149

PTM databases

iPTMnetiP50149
PhosphoSitePlusiP50149

Expressioni

Tissue specificityi

Retinal rod outer segment.

Gene expression databases

BgeeiENSMUSG00000009108 Expressed in 99 organ(s), highest expression level in retina
ExpressionAtlasiP50149 baseline and differential
GenevisibleiP50149 MM

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP50149, 2 interactors
STRINGi10090.ENSMUSP00000053818

Structurei

3D structure databases

ProteinModelPortaliP50149
SMRiP50149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP50149
KOiK04631
OMAiSICNHRF
OrthoDBiEOG091G0VUT
PhylomeDBiP50149
TreeFamiTF300673

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P50149-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSGISAEDK ELARRSKELE KKLQEDADKE AKTVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHQDG YSPEECLEFK SVIYGNVLQS ILAIIRAMST LGIDYAEPSC
110 120 130 140 150
ADAGRQLNNL ADSTEEGTMP PELVDVIRKL WKDGGVQACF DRAAEFQLND
160 170 180 190 200
SASYYLNQLD RITDPNYLPN EQDVLRSRVK TTGIIETKFS VKDLNFRMFD
210 220 230 240 250
VGGQRSERKK WIHCFEGVTC IIFCAALSAY DMVLVEDDEV NRMHESLHLF
260 270 280 290 300
NSICNHKFFA ATSIVLFLNK KDLFEEKIKK VHLSICFPEY DGNNSYEDAG
310 320 330 340 350
NYIKSQFLDL NMRKDVKEIY SHMTCATDTQ NVKFVFDAVT DIIIKENLKD

CGLF
Length:354
Mass (Da):40,118
Last modified:January 23, 2007 - v2
Checksum:i6A14B9B152F14F4E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AE32A2AE32_MOUSE
Guanine nucleotide-binding protein ...
Gnat2
232Annotation score:
F6QPU5F6QPU5_MOUSE
Guanine nucleotide-binding protein ...
Gnat2
249Annotation score:
A2AE31A2AE31_MOUSE
Guanine nucleotide-binding protein ...
Gnat2
112Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10666 mRNA Translation: AAC37650.1
BC016272 mRNA Translation: AAH16272.1
CCDSiCCDS17750.1
RefSeqiNP_032167.1, NM_008141.3
XP_006501071.1, XM_006501008.2
UniGeneiMm.439652

Genome annotation databases

EnsembliENSMUST00000058669; ENSMUSP00000053818; ENSMUSG00000009108
GeneIDi14686
KEGGimmu:14686
UCSCiuc008qyc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10666 mRNA Translation: AAC37650.1
BC016272 mRNA Translation: AAH16272.1
CCDSiCCDS17750.1
RefSeqiNP_032167.1, NM_008141.3
XP_006501071.1, XM_006501008.2
UniGeneiMm.439652

3D structure databases

ProteinModelPortaliP50149
SMRiP50149
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50149, 2 interactors
STRINGi10090.ENSMUSP00000053818

PTM databases

iPTMnetiP50149
PhosphoSitePlusiP50149

Proteomic databases

EPDiP50149
MaxQBiP50149
PaxDbiP50149
PRIDEiP50149

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058669; ENSMUSP00000053818; ENSMUSG00000009108
GeneIDi14686
KEGGimmu:14686
UCSCiuc008qyc.2 mouse

Organism-specific databases

CTDi2780
MGIiMGI:95779 Gnat2

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP50149
KOiK04631
OMAiSICNHRF
OrthoDBiEOG091G0VUT
PhylomeDBiP50149
TreeFamiTF300673

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-4086398 Ca2+ pathway
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Miscellaneous databases

PROiPR:P50149
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009108 Expressed in 99 organ(s), highest expression level in retina
ExpressionAtlasiP50149 baseline and differential
GenevisibleiP50149 MM

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGNAT2_MOUSE
AccessioniPrimary (citable) accession number: P50149
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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