UniProtKB - P50129 (5HT2A_PIG)
Protein
5-hydroxytryptamine receptor 2A
Gene
HTR2A
Organism
Sus scrofa (Pig)
Status
Functioni
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 229 | Hydrophobic barrier that decreases the speed of ligand binding and dissociationBy similarity | 1 |
GO - Molecular functioni
- G protein-coupled serotonin receptor activity Source: InterPro
GO - Biological processi
- behavior Source: UniProtKB-KW
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Behavior |
Names & Taxonomyi
Protein namesi | Recommended name: 5-hydroxytryptamine receptor 2AShort name: 5-HT-2 Short name: 5-HT-2A Short name: 5-HT2A Alternative name(s): Serotonin receptor 2A |
Gene namesi | Name:HTR2A |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- axon By similarity
- Cytoplasmic vesicle By similarity
- caveola By similarity
- dendrite By similarity
- presynapse By similarity
Plasma Membrane
- caveola Source: UniProtKB-SubCell
- integral component of plasma membrane Source: InterPro
Other locations
- axon Source: UniProtKB-SubCell
- cytoplasmic vesicle Source: UniProtKB-KW
- dendrite Source: UniProtKB-SubCell
- presynapse Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 75 | ExtracellularBy similarityAdd BLAST | 75 | |
Transmembranei | 76 – 99 | Helical; Name=1By similarityAdd BLAST | 24 | |
Topological domaini | 100 – 110 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 111 – 132 | Helical; Name=2By similarityAdd BLAST | 22 | |
Topological domaini | 133 – 148 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 149 – 171 | Helical; Name=3By similarityAdd BLAST | 23 | |
Topological domaini | 172 – 191 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 192 – 215 | Helical; Name=4By similarityAdd BLAST | 24 | |
Topological domaini | 216 – 233 | ExtracellularBy similarityAdd BLAST | 18 | |
Transmembranei | 234 – 254 | Helical; Name=5By similarityAdd BLAST | 21 | |
Topological domaini | 255 – 323 | CytoplasmicBy similarityAdd BLAST | 69 | |
Transmembranei | 324 – 345 | Helical; Name=6By similarityAdd BLAST | 22 | |
Topological domaini | 346 – 361 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 362 – 383 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 384 – 470 | CytoplasmicBy similarityAdd BLAST | 87 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068949 | 1 – 470 | 5-hydroxytryptamine receptor 2AAdd BLAST | 470 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 8 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 38 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 44 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 54 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 75 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 148 ↔ 227 | PROSITE-ProRule annotation | ||
Modified residuei | 280 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 348 ↔ 352 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P50129 |
Interactioni
Subunit structurei
Interacts (via C-terminus) with MPDZ and PATJ. May interact (via C-terminus) with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2.
Interacts with GRM2 and DRD2; this may affect signaling.
By similarityProtein-protein interaction databases
STRINGi | 9823.ENSSSCP00000010037 |
Chemistry databases
BindingDBi | P50129 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 155 – 160 | Agonist bindingBy similarity | 6 | |
Regioni | 335 – 339 | Agonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 172 – 174 | DRY motif; important for ligand-induced conformation changesBy similarity | 3 | |
Motifi | 375 – 379 | NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity | 5 | |
Motifi | 468 – 470 | PDZ-bindingBy similarity | 3 |
Domaini
The PDZ domain-binding motif is involved in the interaction with PATJ, CASK, APBA1, DLG1 and DLG4.By similarity
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P50129 |
Family and domain databases
InterProi | View protein in InterPro IPR000455, 5HT2A_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF30, PTHR24247:SF30, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00516, 5HT2ARECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P50129-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVLCEENTS LSSPTNSFMQ LNDDTRLYHN DFNSGEANTS DAFNWTVDSE
60 70 80 90 100
NRTNLSCEGC LSPPCFSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS
110 120 130 140 150
LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV
160 170 180 190 200
WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHRRFNSRT KAFLKIIAVW
210 220 230 240 250
TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM
260 270 280 290 300
VITYFLTIKS LQKEATLCVS DLGTRAKLAS FSFLPQSSLS SEKLFQRSIH
310 320 330 340 350
REPGSYGRRT MQSISNEQKA CKVLGIVFFL FVVMWCPFFI TNIMAVICKE
360 370 380 390 400
SCNEDVIGAL LNVFVWIGYL SSAVNPLVYT LFNKTYRSAF SRYIQCQYKE
410 420 430 440 450
NKKPLQLILV NTIPALAYKS SQLQTGQKEN SKQDDKATEN DCTMVALGKQ
460 470
HSEDAPADNS NTVNEKVSCV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 | R → S in CAA88169 (PubMed:7656980).Curated | 1 | |
Sequence conflicti | 208 | M → K in CAA88169 (PubMed:7656980).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S78208 mRNA Translation: AAB34690.1 Z48152 mRNA Translation: CAA88169.1 |
PIRi | S66489 |
RefSeqi | NP_999382.1, NM_214217.1 |
Genome annotation databases
GeneIDi | 397432 |
KEGGi | ssc:397432 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S78208 mRNA Translation: AAB34690.1 Z48152 mRNA Translation: CAA88169.1 |
PIRi | S66489 |
RefSeqi | NP_999382.1, NM_214217.1 |
3D structure databases
SMRi | P50129 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000010037 |
Chemistry databases
BindingDBi | P50129 |
ChEMBLi | CHEMBL2490 |
DrugCentrali | P50129 |
Protein family/group databases
GPCRDBi | Search... |
Proteomic databases
PaxDbi | P50129 |
Genome annotation databases
GeneIDi | 397432 |
KEGGi | ssc:397432 |
Organism-specific databases
CTDi | 3356 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P50129 |
Miscellaneous databases
PROi | PR:P50129 |
Family and domain databases
InterProi | View protein in InterPro IPR000455, 5HT2A_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF30, PTHR24247:SF30, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00516, 5HT2ARECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5HT2A_PIG | |
Accessioni | P50129Primary (citable) accession number: P50129 Secondary accession number(s): Q29004 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries