UniProtKB - P50086 (PSD10_YEAST)
Protein
Probable 26S proteasome regulatory subunit p28
Gene
NAS6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.3 Publications
Miscellaneous
Present with 319 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- proteasome regulatory particle binding Source: SGD
GO - Biological processi
- proteasome regulatory particle assembly Source: SGD
Keywordsi
Molecular function | Chaperone |
Names & Taxonomyi
Protein namesi | Recommended name: Probable 26S proteasome regulatory subunit p28Alternative name(s): Proteasome non-ATPase subunit 6 |
Gene namesi | Name:NAS6 Ordered Locus Names:YGR232W ORF Names:G8564 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGR232W |
SGDi | S000003464, NAS6 |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000067048 | 1 – 228 | Probable 26S proteasome regulatory subunit p28Add BLAST | 228 |
Proteomic databases
MaxQBi | P50086 |
PaxDbi | P50086 |
PRIDEi | P50086 |
PTM databases
CarbonylDBi | P50086 |
iPTMneti | P50086 |
Interactioni
Subunit structurei
Interacts with RPT3.
1 PublicationBinary interactionsi
Hide detailsP50086
With | #Exp. | IntAct |
---|---|---|
HSM3 [P38348] | 7 | EBI-14028,EBI-21152 |
RPN14 [P53196] | 5 | EBI-14028,EBI-23691 |
RPT3 [P33298] | 10 | EBI-14028,EBI-13905 |
Protein-protein interaction databases
BioGRIDi | 33484, 74 interactors |
DIPi | DIP-1591N |
IntActi | P50086, 29 interactors |
MINTi | P50086 |
STRINGi | 4932.YGR232W |
Miscellaneous databases
RNActi | P50086, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P50086 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P50086 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 1 – 30 | ANK 1Add BLAST | 30 | |
Repeati | 35 – 64 | ANK 2Add BLAST | 30 | |
Repeati | 71 – 100 | ANK 3Add BLAST | 30 | |
Repeati | 106 – 135 | ANK 4Add BLAST | 30 | |
Repeati | 139 – 168 | ANK 5Add BLAST | 30 | |
Repeati | 173 – 203 | ANK 6Add BLAST | 31 |
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG4412, Eukaryota |
GeneTreei | ENSGT00940000153404 |
HOGENOMi | CLU_000134_18_2_1 |
InParanoidi | P50086 |
OMAi | NLKMIQI |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf |
Pfami | View protein in Pfam PF12796, Ank_2, 2 hits |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 6 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 4 hits |
i Sequence
Sequence statusi: Complete.
P50086-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNYPLHQAC MENEFFKVQE LLHSKPSLLL QKDQDGRIPL HWSVSFQAHE
60 70 80 90 100
ITSFLLSKME NVNLDDYPDD SGWTPFHIAC SVGNLEVVKS LYDRPLKPDL
110 120 130 140 150
NKITNQGVTC LHLAVGKKWF EVSQFLIENG ASVRIKDKFN QIPLHRAASV
160 170 180 190 200
GSLKLIELLC GLGKSAVNWQ DKQGWTPLFH ALAEGHGDAA VLLVEKYGAE
210 220
YDLVDNKGAK AEDVALNEQV KKFFLNNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X87941 Genomic DNA Translation: CAA61182.1 Z73017 Genomic DNA Translation: CAA97260.1 AY558275 Genomic DNA Translation: AAS56601.1 BK006941 Genomic DNA Translation: DAA08323.1 |
PIRi | S57697 |
RefSeqi | NP_011748.3, NM_001181361.3 |
Genome annotation databases
EnsemblFungii | YGR232W_mRNA; YGR232W; YGR232W |
GeneIDi | 853147 |
KEGGi | sce:YGR232W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X87941 Genomic DNA Translation: CAA61182.1 Z73017 Genomic DNA Translation: CAA97260.1 AY558275 Genomic DNA Translation: AAS56601.1 BK006941 Genomic DNA Translation: DAA08323.1 |
PIRi | S57697 |
RefSeqi | NP_011748.3, NM_001181361.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IXV | X-ray | 2.30 | A | 1-228 | [»] | |
1WG0 | X-ray | 2.53 | A | 1-228 | [»] | |
2DZN | X-ray | 2.20 | A/C/E | 1-228 | [»] | |
2DZO | X-ray | 3.00 | A/C | 1-228 | [»] | |
SMRi | P50086 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33484, 74 interactors |
DIPi | DIP-1591N |
IntActi | P50086, 29 interactors |
MINTi | P50086 |
STRINGi | 4932.YGR232W |
PTM databases
CarbonylDBi | P50086 |
iPTMneti | P50086 |
Proteomic databases
MaxQBi | P50086 |
PaxDbi | P50086 |
PRIDEi | P50086 |
Genome annotation databases
EnsemblFungii | YGR232W_mRNA; YGR232W; YGR232W |
GeneIDi | 853147 |
KEGGi | sce:YGR232W |
Organism-specific databases
EuPathDBi | FungiDB:YGR232W |
SGDi | S000003464, NAS6 |
Phylogenomic databases
eggNOGi | KOG4412, Eukaryota |
GeneTreei | ENSGT00940000153404 |
HOGENOMi | CLU_000134_18_2_1 |
InParanoidi | P50086 |
OMAi | NLKMIQI |
Miscellaneous databases
EvolutionaryTracei | P50086 |
PROi | PR:P50086 |
RNActi | P50086, protein |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf |
Pfami | View protein in Pfam PF12796, Ank_2, 2 hits |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 6 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSD10_YEAST | |
Accessioni | P50086Primary (citable) accession number: P50086 Secondary accession number(s): D6VV12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names