UniProtKB - P49988 (RP54_PSEAE)
Protein
RNA polymerase sigma-54 factor
Gene
rpoN
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (PubMed:9595661). Plays a role in the regulation of many virulence factors, motility, quorum sensing, mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria, and biofilm formation (PubMed:2152909, PubMed:12644493, PubMed:10792721, PubMed:26633362, PubMed:29760208). Positively controls the T6 secretion system by directly binding to the promoter regions of hcpA and hcpB genes, leading to their expression (PubMed:29760208). Thereby, allows to colonize several hosts efficiently including mammals, insects, nematodes and plants (By similarity) (PubMed:2152909, PubMed:9595661, PubMed:12644493, PubMed:10792721, PubMed:26633362, PubMed:29760208).By similarity6 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 386 – 405 | H-T-H motifSequence analysisAdd BLAST | 20 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: CollecTF
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- sigma factor activity Source: UniProtKB-KW
- transcription regulatory region sequence-specific DNA binding Source: CollecTF
GO - Biological processi
- DNA-templated transcription, initiation Source: InterPro
- negative regulation of single-species biofilm formation Source: PseudoCAP
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Sigma factor, Transferase |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-4551-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase sigma-54 factor |
Gene namesi | Name:rpoN Ordered Locus Names:PA4462 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA4462 |
Subcellular locationi
GO - Cellular componenti
- protein-DNA complex Source: CollecTF
Keywords - Cellular componenti
DNA-directed RNA polymerasePathology & Biotechi
Disruption phenotypei
Deletion mutants result in reduced production of pyocyanine, motility, and proteolytic activity. The motility defect is due to the inability of these mutants to synthesize flagellin (PubMed:2152909). Significantly decreased porin oprE expression under aerobic conditions (PubMed:9595661). Mutants also show defect in biofilm formation (PubMed:29760208).3 Publications
Miscellaneous databases
PHI-basei | PHI:7554 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205534 | 1 – 497 | RNA polymerase sigma-54 factorAdd BLAST | 497 |
Proteomic databases
PaxDbi | P49988 |
PRIDEi | P49988 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 474 – 482 | RPON box | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 35 | Gln-richAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the sigma-54 factor family.Curated
Phylogenomic databases
HOGENOMi | CLU_020569_0_1_6 |
InParanoidi | P49988 |
OMAi | VTTQKFM |
PhylomeDBi | P49988 |
Family and domain databases
Gene3Di | 1.10.10.1330, 1 hit |
InterProi | View protein in InterPro IPR000394, RNA_pol_sigma_54 IPR007046, RNA_pol_sigma_54_core-bd IPR007634, RNA_pol_sigma_54_DNA-bd IPR038709, RpoN_core-bd_sf |
PANTHERi | PTHR32248, PTHR32248, 1 hit |
Pfami | View protein in Pfam PF00309, Sigma54_AID, 1 hit PF04963, Sigma54_CBD, 1 hit PF04552, Sigma54_DBD, 1 hit |
PIRSFi | PIRSF000774, RpoN, 1 hit |
PRINTSi | PR00045, SIGMA54FCT |
TIGRFAMsi | TIGR02395, rpoN_sigma, 1 hit |
PROSITEi | View protein in PROSITE PS00717, SIGMA54_1, 1 hit PS00718, SIGMA54_2, 1 hit PS50044, SIGMA54_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P49988-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ
60 70 80 90 100
EDGDDFDNSD PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS
110 120 130 140 150
ELPVDTAWED IYQTSASSLP SNDDDEWDFT ARTSSGESLH SHLLWQVNLA
160 170 180 190 200
PMSDTDRMIA VTIIDSINND GYLEESLEEI LAAIDPELDV ELDEVEVVLR
210 220 230 240 250
RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV SDYLDLLGGR
260 270 280 290 300
DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK
310 320 330 340 350
DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI
360 370 380 390 400
KSLQSRNETL MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST
410 420 430 440 450
ISRVTTQKYM HTPRGIFELK YFFSSHVSTA EGGECSSTAI RAIIKKLVAA
460 470 480 490
ENAKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 352 | S → T in AAA19793 (PubMed:8113171).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26916 Unassigned DNA Translation: AAA19793.1 AE004091 Genomic DNA Translation: AAG07850.1 |
PIRi | A53373 F83087 |
RefSeqi | NP_253152.1, NC_002516.2 WP_003094357.1, NZ_QZGE01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAG07850; AAG07850; PA4462 |
GeneIDi | 45712433 881022 |
KEGGi | pae:PA4462 |
PATRICi | fig|208964.12.peg.4672 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26916 Unassigned DNA Translation: AAA19793.1 AE004091 Genomic DNA Translation: AAG07850.1 |
PIRi | A53373 F83087 |
RefSeqi | NP_253152.1, NC_002516.2 WP_003094357.1, NZ_QZGE01000004.1 |
3D structure databases
SMRi | P49988 |
ModBasei | Search... |
Protein-protein interaction databases
MINTi | P49988 |
Proteomic databases
PaxDbi | P49988 |
PRIDEi | P49988 |
Genome annotation databases
EnsemblBacteriai | AAG07850; AAG07850; PA4462 |
GeneIDi | 45712433 881022 |
KEGGi | pae:PA4462 |
PATRICi | fig|208964.12.peg.4672 |
Organism-specific databases
PseudoCAPi | PA4462 |
Phylogenomic databases
HOGENOMi | CLU_020569_0_1_6 |
InParanoidi | P49988 |
OMAi | VTTQKFM |
PhylomeDBi | P49988 |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-4551-MONOMER |
Miscellaneous databases
PHI-basei | PHI:7554 |
Gene expression databases
CollecTFi | EXPREG_00000ad0 |
Family and domain databases
Gene3Di | 1.10.10.1330, 1 hit |
InterProi | View protein in InterPro IPR000394, RNA_pol_sigma_54 IPR007046, RNA_pol_sigma_54_core-bd IPR007634, RNA_pol_sigma_54_DNA-bd IPR038709, RpoN_core-bd_sf |
PANTHERi | PTHR32248, PTHR32248, 1 hit |
Pfami | View protein in Pfam PF00309, Sigma54_AID, 1 hit PF04963, Sigma54_CBD, 1 hit PF04552, Sigma54_DBD, 1 hit |
PIRSFi | PIRSF000774, RpoN, 1 hit |
PRINTSi | PR00045, SIGMA54FCT |
TIGRFAMsi | TIGR02395, rpoN_sigma, 1 hit |
PROSITEi | View protein in PROSITE PS00717, SIGMA54_1, 1 hit PS00718, SIGMA54_2, 1 hit PS50044, SIGMA54_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RP54_PSEAE | |
Accessioni | P49988Primary (citable) accession number: P49988 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | December 8, 2000 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families