UniProtKB - P49912 (OPSD_RABIT)
Protein
Rhodopsin
Gene
RHO
Organism
Oryctolagus cuniculus (Rabbit)
Status
Functioni
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 113 | Plays an important role in the conformation switch to the active conformationBy similarity | 1 | |
Metal bindingi | 201 | ZincBy similarity | 1 | |
Metal bindingi | 279 | ZincBy similarity | 1 |
GO - Molecular functioni
- 11-cis retinal binding Source: UniProtKB
- G protein-coupled photoreceptor activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- absorption of visible light Source: AgBase
- photoreceptor cell maintenance Source: Ensembl
- protein-chromophore linkage Source: UniProtKB-KW
- protein phosphorylation Source: Ensembl
- retina development in camera-type eye Source: Ensembl
- rhodopsin mediated signaling pathway Source: UniProtKB
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer |
Biological process | Sensory transduction, Vision |
Ligand | Chromophore, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Rhodopsin |
Gene namesi | Name:RHO |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane By similarity; Multi-pass membrane protein By similarity
- photoreceptor outer segment By similarity
Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia.By similarity
Golgi apparatus
- Golgi apparatus Source: Ensembl
Plasma Membrane
- photoreceptor inner segment membrane Source: UniProtKB
- photoreceptor outer segment membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- cell-cell junction Source: Ensembl
- integral component of membrane Source: UniProtKB
- photoreceptor disc membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 36 | ExtracellularCuratedAdd BLAST | 36 | |
Transmembranei | 37 – 61 | Helical; Name=1By similarityAdd BLAST | 25 | |
Topological domaini | 62 – 73 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 74 – 96 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 97 – 110 | ExtracellularCuratedAdd BLAST | 14 | |
Transmembranei | 111 – 133 | Helical; Name=3By similarityAdd BLAST | 23 | |
Topological domaini | 134 – 152 | CytoplasmicCuratedAdd BLAST | 19 | |
Transmembranei | 153 – 173 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 174 – 202 | ExtracellularCuratedAdd BLAST | 29 | |
Transmembranei | 203 – 224 | Helical; Name=5By similarityAdd BLAST | 22 | |
Topological domaini | 225 – 252 | CytoplasmicCuratedAdd BLAST | 28 | |
Transmembranei | 253 – 274 | Helical; Name=6By similarityAdd BLAST | 22 | |
Topological domaini | 275 – 286 | ExtracellularCuratedAdd BLAST | 12 | |
Transmembranei | 287 – 308 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 309 – 348 | CytoplasmicCuratedAdd BLAST | 40 |
Keywords - Cellular componenti
Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197704 | 1 – 348 | RhodopsinAdd BLAST | 348 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 110 ↔ 187 | PROSITE-ProRule annotation | ||
Modified residuei | 296 | N6-(retinylidene)lysineBy similarity | 1 | |
Lipidationi | 322 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 323 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 334 | PhosphoserineBy similarity | 1 | |
Modified residuei | 336 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 338 | PhosphoserineBy similarity | 1 | |
Modified residuei | 340 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 342 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 343 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.By similarity
Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.By similarity
Keywords - PTMi
Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinExpressioni
Gene expression databases
Bgeei | ENSOCUG00000013036, Expressed in testis and 15 other tissues |
Interactioni
Subunit structurei
Homodimer (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca2+-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).
Interacts with GRK1 (By similarity).
Interacts (phosphorylated form) with SAG.
Interacts with GNAT1.
Interacts with GNAT3. SAG and G-proteins compete for a common binding site (By similarity).
Interacts with PRCD; the interaction promotes PRCD stability.
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000011220 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 330 – 348 | Interaction with SAGBy similarityAdd BLAST | 19 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 134 – 136 | 'Ionic lock' involved in activated form stabilizationBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | P49912 |
OMAi | EQPQYYL |
OrthoDBi | 940057at2759 |
TreeFami | TF324998 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001760, Opsin IPR027430, Retinal_BS IPR000732, Rhodopsin IPR019477, Rhodopsin_N |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit PF10413, Rhodopsin_N, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00238, OPSIN PR00579, RHODOPSIN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P49912-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNGTEGPDFY IPMSNQTGVV RSPFEYPQYY LAEPWQFSML AAYMFLLIVL
60 70 80 90 100
GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVLGG FTTTLYTSLH
110 120 130 140 150
GYFVFGPTGC NVEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE
160 170 180 190 200
NHAIMGVAFT WIMALACAAP PLVGWSRYIP EGMQCSCGID YYTLKPEVNN
210 220 230 240 250
ESFVIYMFVV HFTIPLIIIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV
260 270 280 290 300
TRMVIIMVIA FLICWVPYAS VAFYIFTHQG SNFGPIFMTI PAFFAKSSSI
310 320 330 340
YNPVIYIMMN KQFRNCMLTT ICCGKNPLGD DEASATASKT ETSQVAPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U21688 mRNA Translation: AAA91640.1 |
PIRi | JC4267 |
RefSeqi | NP_001075818.1, NM_001082349.1 |
Genome annotation databases
GeneIDi | 100009200 |
KEGGi | ocu:100009200 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U21688 mRNA Translation: AAA91640.1 |
PIRi | JC4267 |
RefSeqi | NP_001075818.1, NM_001082349.1 |
3D structure databases
SMRi | P49912 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000011220 |
Protein family/group databases
GPCRDBi | Search... |
Genome annotation databases
GeneIDi | 100009200 |
KEGGi | ocu:100009200 |
Organism-specific databases
CTDi | 6010 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | P49912 |
OMAi | EQPQYYL |
OrthoDBi | 940057at2759 |
TreeFami | TF324998 |
Gene expression databases
Bgeei | ENSOCUG00000013036, Expressed in testis and 15 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001760, Opsin IPR027430, Retinal_BS IPR000732, Rhodopsin IPR019477, Rhodopsin_N |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit PF10413, Rhodopsin_N, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00238, OPSIN PR00579, RHODOPSIN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPSD_RABIT | |
Accessioni | P49912Primary (citable) accession number: P49912 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries