UniProtKB - P49903 (SPS1_HUMAN)
Protein
Selenide, water dikinase 1
Gene
SEPHS1
Organism
Homo sapiens (Human)
Status
Functioni
Synthesizes selenophosphate from selenide and ATP.1 Publication
Caution
The conserved active site Cys (or selenocysteine) residue in position 29 is replaced by a Thr. However, as function in selenoprotein synthesis is proven, it is possible Cys-31 is the active site.Curated
Catalytic activityi
- EC:2.7.9.31 Publication
Cofactori
Mg2+1 PublicationNote: Binds 1 Mg2+ ion per monomer.1 Publication
Activity regulationi
Activated by phosphate ions and by potassium ions.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 31 | Sequence analysis | 1 | |
Binding sitei | 32 | ATPCombined sources1 Publication | 1 | |
Sitei | 32 | Important for catalytic activityBy similarity | 1 | |
Metal bindingi | 69 | MagnesiumCombined sources1 Publication | 1 | |
Binding sitei | 87 | ATPCombined sources1 Publication | 1 | |
Metal bindingi | 110 | MagnesiumCombined sources1 Publication | 1 | |
Binding sitei | 110 | ATPCombined sources1 Publication | 1 | |
Metal bindingi | 265 | MagnesiumCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 67 – 69 | ATPCombined sources1 Publication | 3 | |
Nucleotide bindingi | 161 – 164 | ATP; shared with dimeric partnerCombined sources1 Publication | 4 |
GO - Molecular functioni
- ATP binding Source: ProtInc
- GTP binding Source: ProtInc
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- selenide, water dikinase activity Source: GO_Central
GO - Biological processi
- cellular protein modification process Source: ProtInc
- selenocysteine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Selenium |
Enzyme and pathway databases
BRENDAi | 2.7.9.3, 2681 |
PathwayCommonsi | P49903 |
Names & Taxonomyi
Protein namesi | Recommended name: Selenide, water dikinase 1 (EC:2.7.9.31 Publication)Alternative name(s): Selenium donor protein 1 Selenophosphate synthase 1 |
Gene namesi | Name:SEPHS1 Synonyms:SELD, SPS, SPS1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000086475.14 |
HGNCi | HGNC:19685, SEPHS1 |
MIMi | 600902, gene |
neXtProti | NX_P49903 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein Curated
Nucleus
- Nucleus membrane 1 Publication; Peripheral membrane protein Curated
Other locations
- Cytoplasm 1 Publication
Other locations
- Cytoplasm 1 Publication
Other locations
- Cytoplasm 1 Publication
Nucleus
- nuclear membrane Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 85 | T → A: Strongly reduced ADP hydrolysis. 1 Publication | 1 | |
Mutagenesisi | 268 | G → C: No change in ATP-binding. 1 Publication | 1 | |
Mutagenesisi | 270 | G → R: No change in ATP-binding. 1 Publication | 1 | |
Mutagenesisi | 273 | G → A, D or V: Loss of ATP-binding. 1 Publication | 1 | |
Mutagenesisi | 274 | H → N: Reduced ATP-binding. 1 Publication | 1 | |
Mutagenesisi | 274 | H → Y: Increased ATP-binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 22929 |
OpenTargetsi | ENSG00000086475 |
PharmGKBi | PA134905215 |
Miscellaneous databases
Pharosi | P49903, Tbio |
Polymorphism and mutation databases
BioMutai | SEPHS1 |
DMDMi | 27151792 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000127648 | 2 – 392 | Selenide, water dikinase 1Add BLAST | 391 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P49903 |
jPOSTi | P49903 |
MassIVEi | P49903 |
MaxQBi | P49903 |
PaxDbi | P49903 |
PeptideAtlasi | P49903 |
PRIDEi | P49903 |
ProteomicsDBi | 12836 5348 56175 [P49903-1] 64555 |
PTM databases
iPTMneti | P49903 |
MetOSitei | P49903 |
PhosphoSitePlusi | P49903 |
SwissPalmi | P49903 |
Expressioni
Tissue specificityi
Gradually expressed during the cell cycle until G2/M phase and then decreases.1 Publication
Gradually expressed during the cell cycle until G2/M phase and then decreases.1 Publication
Gradually expressed during the cell cycle until S phase and then decreases.1 Publication
Gene expression databases
Bgeei | ENSG00000086475, Expressed in female gonad and 231 other tissues |
ExpressionAtlasi | P49903, baseline and differential |
Genevisiblei | P49903, HS |
Organism-specific databases
HPAi | ENSG00000086475, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP49903
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116589, 58 interactors |
CORUMi | P49903 |
IntActi | P49903, 22 interactors |
MINTi | P49903 |
STRINGi | 9606.ENSP00000367893 |
Miscellaneous databases
RNActi | P49903, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P49903 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P49903 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3939, Eukaryota |
GeneTreei | ENSGT00390000000950 |
HOGENOMi | CLU_032859_1_0_1 |
InParanoidi | P49903 |
OMAi | MNIVCFP |
OrthoDBi | 1166567at2759 |
PhylomeDBi | P49903 |
TreeFami | TF313811 |
Family and domain databases
CDDi | cd02195, SelD, 1 hit |
Gene3Di | 3.30.1330.10, 1 hit 3.90.650.10, 1 hit |
InterProi | View protein in InterPro IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf IPR004536, SPS/SelD |
PANTHERi | PTHR10256, PTHR10256, 1 hit |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 1 hit |
PIRSFi | PIRSF036407, Selenphspht_syn, 1 hit |
SUPFAMi | SSF55326, SSF55326, 1 hit SSF56042, SSF56042, 1 hit |
TIGRFAMsi | TIGR00476, selD, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P49903-1) [UniParc]FASTAAdd to basket
Also known as: Major type, MT
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSTRESFNPE SYELDKSFRL TRFTELKGTG CKVPQDVLQK LLESLQENHF
60 70 80 90 100
QEDEQFLGAV MPRLGIGMDT CVIPLRHGGL SLVQTTDYIY PIVDDPYMMG
110 120 130 140 150
RIACANVLSD LYAMGVTECD NMLMLLGVSN KMTDRERDKV MPLIIQGFKD
160 170 180 190 200
AAEEAGTSVT GGQTVLNPWI VLGGVATTVC QPNEFIMPDN AVPGDVLVLT
210 220 230 240 250
KPLGTQVAVA VHQWLDIPEK WNKIKLVVTQ EDVELAYQEA MMNMARLNRT
260 270 280 290 300
AAGLMHTFNA HAATDITGFG ILGHAQNLAK QQRNEVSFVI HNLPVLAKMA
310 320 330 340 350
AVSKACGNMF GLMHGTCPET SGGLLICLPR EQAARFCAEI KSPKYGEGHQ
360 370 380 390
AWIIGIVEKG NRTARIIDKP RIIEVAPQVA TQNVNPTPGA TS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5T5U6 | Q5T5U6_HUMAN | Selenide, water dikinase 1 | SEPHS1 | 166 | Annotation score: | ||
Q5T5U7 | Q5T5U7_HUMAN | Selenide, water dikinase 1 | SEPHS1 | 91 | Annotation score: |
Sequence cautioni
The sequence AAA87567 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 260 | A → T in AAA87567 (PubMed:7665581).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046701 | 1 – 67 | Missing in isoform 3. 2 PublicationsAdd BLAST | 67 | |
Alternative sequenceiVSP_046702 | 251 – 321 | Missing in isoform 2. 1 PublicationAdd BLAST | 71 | |
Alternative sequenceiVSP_047451 | 322 – 392 | GGLLI…PGATS → DVQ in isoform 4. 1 PublicationAdd BLAST | 71 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000327347; ENSP00000367893; ENSG00000086475 [P49903-1] ENST00000378614; ENSP00000367877; ENSG00000086475 [P49903-2] ENST00000545675; ENSP00000441119; ENSG00000086475 [P49903-3] |
GeneIDi | 22929 |
KEGGi | hsa:22929 |
UCSCi | uc001imk.4, human [P49903-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FD5 | X-ray | 1.90 | A/B | 1-392 | [»] | |
3FD6 | X-ray | 1.95 | A/B | 1-392 | [»] | |
SMRi | P49903 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116589, 58 interactors |
CORUMi | P49903 |
IntActi | P49903, 22 interactors |
MINTi | P49903 |
STRINGi | 9606.ENSP00000367893 |
PTM databases
iPTMneti | P49903 |
MetOSitei | P49903 |
PhosphoSitePlusi | P49903 |
SwissPalmi | P49903 |
Polymorphism and mutation databases
BioMutai | SEPHS1 |
DMDMi | 27151792 |
Proteomic databases
EPDi | P49903 |
jPOSTi | P49903 |
MassIVEi | P49903 |
MaxQBi | P49903 |
PaxDbi | P49903 |
PeptideAtlasi | P49903 |
PRIDEi | P49903 |
ProteomicsDBi | 12836 5348 56175 [P49903-1] 64555 |
Protocols and materials databases
Antibodypediai | 24810, 184 antibodies |
DNASUi | 22929 |
Genome annotation databases
Ensembli | ENST00000327347; ENSP00000367893; ENSG00000086475 [P49903-1] ENST00000378614; ENSP00000367877; ENSG00000086475 [P49903-2] ENST00000545675; ENSP00000441119; ENSG00000086475 [P49903-3] |
GeneIDi | 22929 |
KEGGi | hsa:22929 |
UCSCi | uc001imk.4, human [P49903-1] |
Organism-specific databases
CTDi | 22929 |
DisGeNETi | 22929 |
EuPathDBi | HostDB:ENSG00000086475.14 |
GeneCardsi | SEPHS1 |
HGNCi | HGNC:19685, SEPHS1 |
HPAi | ENSG00000086475, Low tissue specificity |
MIMi | 600902, gene |
neXtProti | NX_P49903 |
OpenTargetsi | ENSG00000086475 |
PharmGKBi | PA134905215 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3939, Eukaryota |
GeneTreei | ENSGT00390000000950 |
HOGENOMi | CLU_032859_1_0_1 |
InParanoidi | P49903 |
OMAi | MNIVCFP |
OrthoDBi | 1166567at2759 |
PhylomeDBi | P49903 |
TreeFami | TF313811 |
Enzyme and pathway databases
BRENDAi | 2.7.9.3, 2681 |
PathwayCommonsi | P49903 |
Miscellaneous databases
BioGRID-ORCSi | 22929, 117 hits in 844 CRISPR screens |
ChiTaRSi | SEPHS1, human |
EvolutionaryTracei | P49903 |
GeneWikii | Selenophosphate_synthetase_1 |
GenomeRNAii | 22929 |
Pharosi | P49903, Tbio |
PROi | PR:P49903 |
RNActi | P49903, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000086475, Expressed in female gonad and 231 other tissues |
ExpressionAtlasi | P49903, baseline and differential |
Genevisiblei | P49903, HS |
Family and domain databases
CDDi | cd02195, SelD, 1 hit |
Gene3Di | 3.30.1330.10, 1 hit 3.90.650.10, 1 hit |
InterProi | View protein in InterPro IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf IPR004536, SPS/SelD |
PANTHERi | PTHR10256, PTHR10256, 1 hit |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 1 hit |
PIRSFi | PIRSF036407, Selenphspht_syn, 1 hit |
SUPFAMi | SSF55326, SSF55326, 1 hit SSF56042, SSF56042, 1 hit |
TIGRFAMsi | TIGR00476, selD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPS1_HUMAN | |
Accessioni | P49903Primary (citable) accession number: P49903 Secondary accession number(s): B4DWK0 Q9BVT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | December 13, 2002 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM