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Protein

Estrogen sulfotransferase, isoform 3

Gene

Ste

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108Proton acceptorBy similarity1
Binding sitei130PAPSBy similarity1
Binding sitei138PAPSBy similarity1
Binding sitei193PAPSBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 53PAPSBy similarity6
Nucleotide bindingi227 – 232PAPSBy similarity6
Nucleotide bindingi257 – 259PAPSBy similarity3

GO - Molecular functioni

  • estrone sulfotransferase activity Source: RGD
  • steroid binding Source: UniProtKB-KW

GO - Biological processi

  • estrogen metabolic process Source: RGD

Keywordsi

Molecular functionTransferase
LigandLipid-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Estrogen sulfotransferase, isoform 3 (EC:2.8.2.4)
Short name:
EST-3
Alternative name(s):
Estrone sulfotransferase
Sulfotransferase, estrogen-preferring
Gene namesi
Name:Ste
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3776 Ste

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4831

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851571 – 295Estrogen sulfotransferase, isoform 3Add BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP49889

PTM databases

iPTMnetiP49889
PhosphoSitePlusiP49889

Expressioni

Tissue specificityi

Liver of young mature males and uterus.

Developmental stagei

Expressed only in the liver of young adult animals (100 days old) and is absent in the prepubertal male (27 days old), senescent male (800 days old) and female liver.

Inductioni

Induced by androgens and suppressed by estrogens. The expression is under the influence of pituitary growth hormone and thyroid hormone. Is regulated by progesterone in the uterus.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP49889
SMRiP49889
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 108Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG1584 Eukaryota
ENOG4111H56 LUCA
HOVERGENiHBG001195
InParanoidiP49889
KOiK01016
PhylomeDBiP49889

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

P49889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METSMPEYYD VFGDFHGFLM DKRFTKYWED VETFLARPDD LLIVTYPKSG
60 70 80 90 100
STWISEIVDM IYKEGDVEKC KEDALFNRIP DLECRNEDLI NGIKQLKEKE
110 120 130 140 150
SPRIVKTHLP AKLLPASFWE KNCKIIYLCR NAKDVVVSYY YFFLIMKSYP
160 170 180 190 200
NPKSFSEFVE KFMEGQVPYG SWYDHVKSWW EKSKNSRVLF MFYEDMKEDI
210 220 230 240 250
RREVVKLIEF LERDPSAELV DRIIQHTSFQ EMKNNPCTNY SMLPETMIDL
260 270 280 290
KVSPFMRKGI VGDWKNHFPE ALRERFEEHY QQQMKDCPVK FRAEL
Length:295
Mass (Da):35,416
Last modified:October 1, 1996 - v1
Checksum:iA77807A21DD2E7EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150P → Q in AAB33441 (PubMed:7857871).Curated1
Sequence conflicti238T → I in AAB33441 (PubMed:7857871).Curated1
Sequence conflicti295L → P in AAB33441 (PubMed:7857871).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86758 mRNA Translation: AAA41128.1
S76489 mRNA Translation: AAB33441.1
AJ131835
, AJ298109, AJ298110, AJ298111, AJ298112, AJ298113, AJ298114 Genomic DNA Translation: CAA10515.2
PIRiA41930
I56606
RefSeqiNP_037015.1, NM_012883.1
UniGeneiRn.32104

Genome annotation databases

GeneIDi360268
KEGGirno:25355
UCSCiRGD:3776 rat

Similar proteinsi

Entry informationi

Entry nameiST1E3_RAT
AccessioniPrimary (citable) accession number: P49889
Secondary accession number(s): Q9QWS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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