UniProtKB - P49888 (ST1E1_HUMAN)
Sulfotransferase 1E1
SULT1E1
Functioni
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone (PubMed:7779757, PubMed:11884392, PubMed:11006110).
Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency (PubMed:11006110, PubMed:19589875).
Does not sulfonate cortisol, testosterone and dopamine (PubMed:7779757, PubMed:11006110).
May play a role in gut microbiota-host metabolic interaction. O-sulfonates 4-ethylphenol (4-EP), a dietary tyrosine-derived metabolite produced by gut bacteria. The product 4-EPS crosses the blood-brain barrier and may negatively regulate oligodendrocyte maturation and myelination, affecting the functional connectivity of different brain regions associated with the limbic system.
5 PublicationsCatalytic activityi
- 3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate + H+3 PublicationsEC:2.8.2.43 PublicationsThis reaction proceeds in the forward1 Publication direction.
- (24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3-sulfate + adenosine 3',5'-bisphosphate + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 17β-estradiol + 3'-phosphoadenylyl sulfate = 17β-estradiol 3-sulfate + adenosine 3',5'-bisphosphate + H+2 PublicationsThis reaction proceeds in the forward1 Publication direction.
- 3'-phosphoadenylyl sulfate + 4-ethylphenol = 4-ethylphenyl sulfate + adenosine 3',5'-bisphosphate + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Kineticsi
- KM=0.3 µM for 17beta-estradiol1 Publication
- KM=0.2 µM for estrone1 Publication
- KM=0.2 µM for DHEA1 Publication
- KM=32220 µM for dopamine1 Publication
- KM=76.6 µM for p-nitrophenol1 Publication
- KM=0.9 µM for (24S)-hydroxycholesterol1 Publication
- KM=0.66 µM for PAPS1 Publication
- KM=0.46 µM for PAPS1 Publication
- Vmax=37.2 nmol/min/mg enzyme with 17beta-estradiol1 Publication
- Vmax=16.3 nmol/min/mg enzyme with estrone1 Publication
- Vmax=5.5 nmol/min/mg enzyme with DHEA1 Publication
- Vmax=13.3 nmol/min/mg enzyme with dopaminne
- Vmax=135.9 nmol/min/mg enzyme with p-nitrophenol1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 107 | Proton acceptor1 Publication | 1 | |
Binding sitei | 129 | PAPSBy similarity | 1 | |
Binding sitei | 137 | PAPS1 Publication | 1 | |
Binding sitei | 192 | PAPSBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 47 – 52 | PAPSBy similarity | 6 | |
Nucleotide bindingi | 226 – 231 | PAPSBy similarity | 6 | |
Nucleotide bindingi | 256 – 258 | PAPSBy similarity | 3 |
GO - Molecular functioni
- aryl sulfotransferase activity Source: GO_Central
- estrone sulfotransferase activity Source: ProtInc
- flavonol 3-sulfotransferase activity Source: BHF-UCL
- steroid binding Source: UniProtKB-KW
- steroid sulfotransferase activity Source: UniProtKB
- sulfotransferase activity Source: MGI
GO - Biological processi
- 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: CAFA
- estrogen catabolic process Source: CAFA
- estrogen metabolic process Source: UniProtKB
- ethanol catabolic process Source: CAFA
- positive regulation of fat cell differentiation Source: CACAO
- steroid metabolic process Source: ProtInc
- sulfation Source: BHF-UCL
Keywordsi
Molecular function | Transferase |
Biological process | Lipid metabolism |
Ligand | Lipid-binding, Steroid-binding |
Enzyme and pathway databases
BRENDAi | 2.8.2.4, 2681 |
PathwayCommonsi | P49888 |
Reactomei | R-HSA-156584, Cytosolic sulfonation of small molecules |
SABIO-RKi | P49888 |
SignaLinki | P49888 |
SIGNORi | P49888 |
Chemistry databases
SwissLipidsi | SLP:000001693 |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfotransferase 1E1 (EC:2.8.2.42 Publications)Short name: ST1E1 Alternative name(s): EST-11 Publication Estrogen sulfotransferase1 Publication Sulfotransferase, estrogen-preferring |
Gene namesi | Name:SULT1E1 Synonyms:STE |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11377, SULT1E1 |
MIMi | 600043, gene |
neXtProti | NX_P49888 |
VEuPathDBi | HostDB:ENSG00000109193 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear membrane Source: HPA
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 85 | K → A: Does not have decreased sulfonation activity towards the estrogens and DHEA. 1 Publication | 1 | |
Mutagenesisi | 107 | H → A: Complete loss of sulfonation activity towards all substrates tested. 1 Publication | 1 | |
Mutagenesisi | 137 | S → A: Decreased gradually the sulfotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 137 | S → C: Decreased gradually the sulfotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 145 | V → E: Substrate specificity constants for estradiol and estrone are reduced. Dramatic 400-fold increase in the ability to sulfonate dopamine. 1 Publication | 1 | |
Mutagenesisi | 269 | V → E: Does not prevent the formation of homodimer. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6783 |
OpenTargetsi | ENSG00000109193 |
PharmGKBi | PA340 |
Miscellaneous databases
Pharosi | P49888, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2346 |
DrugBanki | DB02902, 3'-phospho-5'-adenylyl sulfate DB03346, 3,3',5,5'-tetrachlorobiphenyl-4,4'-diol DB01812, Adenosine 3',5'-diphosphate DB00714, Apomorphine DB14635, Curcumin sulfate DB01176, Cyclizine DB00977, Ethinylestradiol DB09288, Propacetamol DB00675, Tamoxifen DB09100, Thyroid, porcine |
Genetic variation databases
BioMutai | SULT1E1 |
DMDMi | 1711604 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085153 | 1 – 294 | Sulfotransferase 1E1Add BLAST | 294 |
Proteomic databases
EPDi | P49888 |
MassIVEi | P49888 |
PaxDbi | P49888 |
PeptideAtlasi | P49888 |
PRIDEi | P49888 |
ProteomicsDBi | 56163 |
PTM databases
iPTMneti | P49888 |
PhosphoSitePlusi | P49888 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000109193, Expressed in kidney and 122 other tissues |
ExpressionAtlasi | P49888, baseline and differential |
Genevisiblei | P49888, HS |
Organism-specific databases
HPAi | ENSG00000109193, Tissue enhanced (intestine, liver, skin, vagina) |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsBinary interactionsi
P49888
With | #Exp. | IntAct |
---|---|---|
PDE9A [O76083] | 3 | EBI-712512,EBI-742764 |
PDE9A - isoform PDE9A2 [O76083-2] | 3 | EBI-712512,EBI-11524542 |
Protein-protein interaction databases
BioGRIDi | 112660, 14 interactors |
IntActi | P49888, 12 interactors |
STRINGi | 9606.ENSP00000226444 |
Chemistry databases
BindingDBi | P49888 |
Miscellaneous databases
RNActi | P49888, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P49888 |
SMRi | P49888 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P49888 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 105 – 107 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1584, Eukaryota |
GeneTreei | ENSGT00940000162261 |
HOGENOMi | CLU_027239_1_2_1 |
InParanoidi | P49888 |
OMAi | VIKVIQF |
OrthoDBi | 780670at2759 |
PhylomeDBi | P49888 |
TreeFami | TF321745 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNSELDYYEK FEEVHGILMY KDFVKYWDNV EAFQARPDDL VIATYPKSGT
60 70 80 90 100
TWVSEIVYMI YKEGDVEKCK EDVIFNRIPF LECRKENLMN GVKQLDEMNS
110 120 130 140 150
PRIVKTHLPP ELLPASFWEK DCKIIYLCRN AKDVAVSFYY FFLMVAGHPN
160 170 180 190 200
PGSFPEFVEK FMQGQVPYGS WYKHVKSWWE KGKSPRVLFL FYEDLKEDIR
210 220 230 240 250
KEVIKLIHFL ERKPSEELVD RIIHHTSFQE MKNNPSTNYT TLPDEIMNQK
260 270 280 290
LSPFMRKGIT GDWKNHFTVA LNEKFDKHYE QQMKESTLKF RTEI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 154 | F → L in AAH27956 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_018907 | 22 | D → Y1 PublicationCorresponds to variant dbSNP:rs11569705Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08098 mRNA Translation: AAA82125.1 U20521 , U20515, U20516, U20517, U20518, U20519, U20520 Genomic DNA Translation: AAC50286.1 S77383 mRNA Translation: AAB34601.1 Y11195 mRNA Translation: CAA72079.1 AY436634 Genomic DNA Translation: AAQ97179.1 BC027956 mRNA Translation: AAH27956.1 U55764 mRNA Translation: AAB51658.1 |
CCDSi | CCDS3531.1 |
PIRi | JC2229 |
RefSeqi | NP_005411.1, NM_005420.2 |
Genome annotation databases
Ensembli | ENST00000226444.4; ENSP00000226444.3; ENSG00000109193.12 |
GeneIDi | 6783 |
KEGGi | hsa:6783 |
MANE-Selecti | ENST00000226444.4; ENSP00000226444.3; NM_005420.3; NP_005411.1 |
Similar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08098 mRNA Translation: AAA82125.1 U20521 , U20515, U20516, U20517, U20518, U20519, U20520 Genomic DNA Translation: AAC50286.1 S77383 mRNA Translation: AAB34601.1 Y11195 mRNA Translation: CAA72079.1 AY436634 Genomic DNA Translation: AAQ97179.1 BC027956 mRNA Translation: AAH27956.1 U55764 mRNA Translation: AAB51658.1 |
CCDSi | CCDS3531.1 |
PIRi | JC2229 |
RefSeqi | NP_005411.1, NM_005420.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G3M | X-ray | 1.70 | A/B | 1-294 | [»] | |
1HY3 | X-ray | 1.80 | A/B | 1-294 | [»] | |
4JVL | X-ray | 1.94 | A/B | 1-294 | [»] | |
4JVM | X-ray | 1.99 | A/B | 1-294 | [»] | |
4JVN | X-ray | 2.05 | A/B | 1-294 | [»] | |
AlphaFoldDBi | P49888 | |||||
SMRi | P49888 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112660, 14 interactors |
IntActi | P49888, 12 interactors |
STRINGi | 9606.ENSP00000226444 |
Chemistry databases
BindingDBi | P49888 |
ChEMBLi | CHEMBL2346 |
DrugBanki | DB02902, 3'-phospho-5'-adenylyl sulfate DB03346, 3,3',5,5'-tetrachlorobiphenyl-4,4'-diol DB01812, Adenosine 3',5'-diphosphate DB00714, Apomorphine DB14635, Curcumin sulfate DB01176, Cyclizine DB00977, Ethinylestradiol DB09288, Propacetamol DB00675, Tamoxifen DB09100, Thyroid, porcine |
SwissLipidsi | SLP:000001693 |
PTM databases
iPTMneti | P49888 |
PhosphoSitePlusi | P49888 |
Genetic variation databases
BioMutai | SULT1E1 |
DMDMi | 1711604 |
Proteomic databases
EPDi | P49888 |
MassIVEi | P49888 |
PaxDbi | P49888 |
PeptideAtlasi | P49888 |
PRIDEi | P49888 |
ProteomicsDBi | 56163 |
Protocols and materials databases
Antibodypediai | 24249, 293 antibodies from 34 providers |
CPTCi | P49888, 3 antibodies |
DNASUi | 6783 |
Genome annotation databases
Ensembli | ENST00000226444.4; ENSP00000226444.3; ENSG00000109193.12 |
GeneIDi | 6783 |
KEGGi | hsa:6783 |
MANE-Selecti | ENST00000226444.4; ENSP00000226444.3; NM_005420.3; NP_005411.1 |
Organism-specific databases
CTDi | 6783 |
DisGeNETi | 6783 |
GeneCardsi | SULT1E1 |
HGNCi | HGNC:11377, SULT1E1 |
HPAi | ENSG00000109193, Tissue enhanced (intestine, liver, skin, vagina) |
MIMi | 600043, gene |
neXtProti | NX_P49888 |
OpenTargetsi | ENSG00000109193 |
PharmGKBi | PA340 |
VEuPathDBi | HostDB:ENSG00000109193 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1584, Eukaryota |
GeneTreei | ENSGT00940000162261 |
HOGENOMi | CLU_027239_1_2_1 |
InParanoidi | P49888 |
OMAi | VIKVIQF |
OrthoDBi | 780670at2759 |
PhylomeDBi | P49888 |
TreeFami | TF321745 |
Enzyme and pathway databases
BRENDAi | 2.8.2.4, 2681 |
PathwayCommonsi | P49888 |
Reactomei | R-HSA-156584, Cytosolic sulfonation of small molecules |
SABIO-RKi | P49888 |
SignaLinki | P49888 |
SIGNORi | P49888 |
Miscellaneous databases
BioGRID-ORCSi | 6783, 76 hits in 1076 CRISPR screens |
EvolutionaryTracei | P49888 |
GeneWikii | SULT1E1 |
GenomeRNAii | 6783 |
Pharosi | P49888, Tchem |
PROi | PR:P49888 |
RNActi | P49888, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000109193, Expressed in kidney and 122 other tissues |
ExpressionAtlasi | P49888, baseline and differential |
Genevisiblei | P49888, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ST1E1_HUMAN | |
Accessioni | P49888Primary (citable) accession number: P49888 Secondary accession number(s): Q8N6X5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families