UniProtKB - P49872 (F261_BOVIN)
Protein
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1
Gene
PFKFB1
Organism
Bos taurus (Bovine)
Status
Functioni
Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activityi
Activity regulationi
Phosphorylation at Ser-33 inhibits the kinase and activates the bisphosphatase.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 82 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 105 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 131 | Sequence analysis | 1 | |
Binding sitei | 133 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 139 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 161 | Sequence analysis | 1 | |
Binding sitei | 175 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 196 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 200 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 258 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Active sitei | 259 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Binding sitei | 265 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 271 | Fructose 2,6-bisphosphate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 308 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Active sitei | 328 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 339 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 353 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 357 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 368 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 393 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 394 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 398 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 430 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 49 – 57 | ATPBy similarity | 9 | |
Nucleotide bindingi | 170 – 175 | ATPBy similarity | 6 | |
Nucleotide bindingi | 350 – 353 | ATPBy similarity | 4 | |
Nucleotide bindingi | 394 – 398 | ATPBy similarity | 5 |
GO - Molecular functioni
- 6-phosphofructo-2-kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- fructose-2,6-bisphosphate 2-phosphatase activity Source: UniProtKB
GO - Biological processi
- fructose 2,6-bisphosphate metabolic process Source: UniProtKB
- fructose metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Kinase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.46, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1Short name: 6PF-2-K/Fru-2,6-P2ase 1 Short name: PFK/FBPase 1 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase liver isozyme Including the following 2 domains: Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
Gene namesi | Name:PFKFB1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Cytosol
- 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex Source: UniProtKB
- cytosol Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000179959 | 2 – 471 | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1Add BLAST | 470 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 33 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 141 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P49872 |
Expressioni
Tissue specificityi
Liver.
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000000198 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 250 | 6-phosphofructo-2-kinaseAdd BLAST | 249 | |
Regioni | 251 – 471 | Fructose-2,6-bisphosphataseAdd BLAST | 221 |
Sequence similaritiesi
In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
InParanoidi | P49872 |
OrthoDBi | 392001at2759 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P49872-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQEMGELTQ TRLQKIWIPH NNGNSRLQRR RGSSIPQFTN SPTMVIMVGL
60 70 80 90 100
PARGKTYIST KLTRYLNWIG TPTKVFNLGQ YRREAVSYKN YEFFLPDNME
110 120 130 140 150
ALLIRKQCAL AALKDVHSYL SHEEGRVAVF DATNTTRERR SLILQFAKEH
160 170 180 190 200
GYKVFFIESI CNDPDVIAEN IRQVKLGSPD YIDCDREKVL EDFLKRIECY
210 220 230 240 250
EVNYQPLDDE LDSHLSYIKI FDVGTRYMVN RVQDHIQSRT VYYLMNIHVT
260 270 280 290 300
PRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IQSQGISSLK
310 320 330 340 350
VGTSHMKRTI QTAEALGLPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF
360 370 380 390 400
ALRDQDKYRY RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL
410 420 430 440 450
LAYFLDKSSD ELPYLKCPLH TVLKLTPVAY GCKVESIYLN VEAVNTHREK
460 470
PENVDITREP EEALDTVPAH Y
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64323 mRNA Translation: AAA30696.1 S55569 mRNA Translation: AAB19845.1 |
PIRi | A44872 |
RefSeqi | NP_776997.3, NM_174572.4 |
Genome annotation databases
GeneIDi | 282304 |
KEGGi | bta:282304 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64323 mRNA Translation: AAA30696.1 S55569 mRNA Translation: AAB19845.1 |
PIRi | A44872 |
RefSeqi | NP_776997.3, NM_174572.4 |
3D structure databases
BMRBi | P49872 |
SMRi | P49872 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000000198 |
Proteomic databases
PaxDbi | P49872 |
Genome annotation databases
GeneIDi | 282304 |
KEGGi | bta:282304 |
Organism-specific databases
CTDi | 5207 |
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
InParanoidi | P49872 |
OrthoDBi | 392001at2759 |
Enzyme and pathway databases
BRENDAi | 3.1.3.46, 908 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F261_BOVIN | |
Accessioni | P49872Primary (citable) accession number: P49872 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families