UniProtKB - P49862 (KLK7_HUMAN)
Kallikrein-7
KLK7
Functioni
May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
1 PublicationCatalytic activityi
- Cleavage of proteins with aromatic side chains in the P1 position. EC:3.4.21.117
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 70 | Charge relay system1 Publication | 1 | |
Sitei | 109 | Major binding site for inhibitory zinc or copper | 1 | |
Active sitei | 112 | Charge relay system1 Publication | 1 | |
Active sitei | 205 | Charge relay system1 Publication | 1 |
GO - Molecular functioni
- metalloendopeptidase activity Source: Reactome
- peptidase activity Source: UniProtKB
- serine-type endopeptidase activity Source: InterPro
- serine-type peptidase activity Source: ProtInc
GO - Biological processi
- epidermis development Source: ProtInc
- extracellular matrix disassembly Source: Reactome
- positive regulation of antibacterial peptide production Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.117, 2681 |
PathwayCommonsi | P49862 |
Reactomei | R-HSA-1474228, Degradation of the extracellular matrix |
SignaLinki | P49862 |
Protein family/group databases
MEROPSi | S01.300 |
Names & Taxonomyi
Protein namesi | Recommended name: Kallikrein-7 (EC:3.4.21.117)Short name: hK7 Alternative name(s): Serine protease 6 Stratum corneum chymotryptic enzyme Short name: hSCCE |
Gene namesi | Name:KLK7 Synonyms:PRSS6, SCCE |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6368, KLK7 |
MIMi | 604438, gene |
neXtProti | NX_P49862 |
VEuPathDBi | HostDB:ENSG00000169035 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Note: In ovarian carcinoma, secreted and also observed at the apical membrane and in cytoplasm at the invasive front.
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Other locations
- epidermal lamellar body Source: UniProtKB
- secretory granule Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | H → F: No effect on zinc inhibition. 1 Publication | 1 | |
Mutagenesisi | 109 | H → A: No zinc inhibition. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5650 |
OpenTargetsi | ENSG00000169035 |
PharmGKBi | PA30157 |
Miscellaneous databases
Pharosi | P49862, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2443 |
DrugBanki | DB08038, L-alanyl-N-[(1S,2R)-1-benzyl-2-hydroxypropyl]-L-alaninamide |
GuidetoPHARMACOLOGYi | 2377 |
Genetic variation databases
BioMutai | KLK7 |
DMDMi | 1710878 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 1 PublicationAdd BLAST | 22 | |
PropeptideiPRO_0000027942 | 23 – 29 | Activation peptide | 7 | |
ChainiPRO_0000027943 | 30 – 253 | Kallikrein-7Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 36 ↔ 165 | |||
Disulfide bondi | 55 ↔ 71 | |||
Disulfide bondi | 137 ↔ 239 | |||
Disulfide bondi | 144 ↔ 211 | |||
Disulfide bondi | 176 ↔ 190 | |||
Disulfide bondi | 201 ↔ 226 | |||
Glycosylationi | 246 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
EPDi | P49862 |
MassIVEi | P49862 |
PaxDbi | P49862 |
PeptideAtlasi | P49862 |
PRIDEi | P49862 |
ProteomicsDBi | 56160 [P49862-1] 56161 [P49862-2] |
PTM databases
GlyGeni | P49862, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | P49862 |
PhosphoSitePlusi | P49862 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000169035, Expressed in mammalian vulva and 185 other tissues |
ExpressionAtlasi | P49862, baseline and differential |
Genevisiblei | P49862, HS |
Organism-specific databases
HPAi | ENSG00000169035, Group enriched (esophagus, skin) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 111631, 72 interactors |
IntActi | P49862, 23 interactors |
MINTi | P49862 |
STRINGi | 9606.ENSP00000375683 |
Chemistry databases
BindingDBi | P49862 |
Miscellaneous databases
RNActi | P49862, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P49862 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P49862 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 250 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01020000230389 |
HOGENOMi | CLU_006842_1_1_1 |
InParanoidi | P49862 |
OMAi | NAWRGDS |
PhylomeDBi | P49862 |
TreeFami | TF331065 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MARSLLLPLQ ILLLSLALET AGEEAQGDKI IDGAPCARGS HPWQVALLSG
60 70 80 90 100
NQLHCGGVLV NERWVLTAAH CKMNEYTVHL GSDTLGDRRA QRIKASKSFR
110 120 130 140 150
HPGYSTQTHV NDLMLVKLNS QARLSSMVKK VRLPSRCEPP GTTCTVSGWG
160 170 180 190 200
TTTSPDVTFP SDLMCVDVKL ISPQDCTKVY KDLLENSMLC AGIPDSKKNA
210 220 230 240 250
CNGDSGGPLV CRGTLQGLVS WGTFPCGQPN DPGVYTQVCK FTKWINDTMK
KHR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ6DTY1 | Q6DTY1_HUMAN | Kallikrein 7 splice variant 3 | KLK7 | 66 | Annotation score: | ||
M0QYU8 | M0QYU8_HUMAN | Kallikrein-7 | KLK7 | 69 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 226 | C → W in AAH32005 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013581 | 1 – 72 | Missing in isoform 2. 1 PublicationAdd BLAST | 72 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33404 mRNA Translation: AAC37551.1 AF166330 Genomic DNA Translation: AAD49718.1 AF243527 Genomic DNA Translation: AAG33360.1 AF332583 Genomic DNA Translation: AAK69624.1 AF411214 mRNA Translation: AAN03662.1 AF411215 mRNA Translation: AAN03663.1 AY601109 mRNA Translation: AAU04540.1 AK289660 mRNA Translation: BAF82349.1 CH471135 Genomic DNA Translation: EAW71959.1 BC032005 mRNA Translation: AAH32005.1 |
CCDSi | CCDS12812.1 [P49862-1] CCDS59414.1 [P49862-2] |
PIRi | A53968 |
RefSeqi | NP_001193982.1, NM_001207053.1 [P49862-2] NP_001230055.1, NM_001243126.1 NP_005037.1, NM_005046.3 [P49862-1] NP_644806.1, NM_139277.2 [P49862-1] |
Genome annotation databases
Ensembli | ENST00000391807; ENSP00000375683; ENSG00000169035 ENST00000595820; ENSP00000470538; ENSG00000169035 ENST00000597707; ENSP00000469950; ENSG00000169035 [P49862-2] |
GeneIDi | 5650 |
KEGGi | hsa:5650 |
MANE-Selecti | ENST00000595820.6; ENSP00000470538.1; NM_005046.4; NP_005037.1 |
UCSCi | uc002puo.4, human [P49862-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33404 mRNA Translation: AAC37551.1 AF166330 Genomic DNA Translation: AAD49718.1 AF243527 Genomic DNA Translation: AAG33360.1 AF332583 Genomic DNA Translation: AAK69624.1 AF411214 mRNA Translation: AAN03662.1 AF411215 mRNA Translation: AAN03663.1 AY601109 mRNA Translation: AAU04540.1 AK289660 mRNA Translation: BAF82349.1 CH471135 Genomic DNA Translation: EAW71959.1 BC032005 mRNA Translation: AAH32005.1 |
CCDSi | CCDS12812.1 [P49862-1] CCDS59414.1 [P49862-2] |
PIRi | A53968 |
RefSeqi | NP_001193982.1, NM_001207053.1 [P49862-2] NP_001230055.1, NM_001243126.1 NP_005037.1, NM_005046.3 [P49862-1] NP_644806.1, NM_139277.2 [P49862-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QXG | X-ray | 2.60 | A/B | 30-253 | [»] | |
2QXH | X-ray | 2.00 | A | 30-253 | [»] | |
2QXI | X-ray | 1.00 | A | 30-253 | [»] | |
2QXJ | X-ray | 2.10 | A | 30-253 | [»] | |
3BSQ | X-ray | 2.80 | A/B/C | 30-250 | [»] | |
5FAH | X-ray | 1.10 | A | 30-253 | [»] | |
5Y9L | X-ray | 2.15 | A | 30-253 | [»] | |
5YJK | X-ray | 2.40 | A | 30-253 | [»] | |
6SHH | X-ray | 2.00 | A/B/C/D/E/F/G/H | 30-253 | [»] | |
6SHI | X-ray | 1.85 | A/B/C/D/E/F/G/H | 30-253 | [»] | |
6SJU | X-ray | 1.97 | A/B/C/D/E/F/G/H | 30-253 | [»] | |
6Y4S | X-ray | 2.23 | A/B/C | 30-253 | [»] | |
SMRi | P49862 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111631, 72 interactors |
IntActi | P49862, 23 interactors |
MINTi | P49862 |
STRINGi | 9606.ENSP00000375683 |
Chemistry databases
BindingDBi | P49862 |
ChEMBLi | CHEMBL2443 |
DrugBanki | DB08038, L-alanyl-N-[(1S,2R)-1-benzyl-2-hydroxypropyl]-L-alaninamide |
GuidetoPHARMACOLOGYi | 2377 |
Protein family/group databases
MEROPSi | S01.300 |
PTM databases
GlyGeni | P49862, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | P49862 |
PhosphoSitePlusi | P49862 |
Genetic variation databases
BioMutai | KLK7 |
DMDMi | 1710878 |
Proteomic databases
EPDi | P49862 |
MassIVEi | P49862 |
PaxDbi | P49862 |
PeptideAtlasi | P49862 |
PRIDEi | P49862 |
ProteomicsDBi | 56160 [P49862-1] 56161 [P49862-2] |
Protocols and materials databases
Antibodypediai | 18939, 414 antibodies from 31 providers |
DNASUi | 5650 |
Genome annotation databases
Ensembli | ENST00000391807; ENSP00000375683; ENSG00000169035 ENST00000595820; ENSP00000470538; ENSG00000169035 ENST00000597707; ENSP00000469950; ENSG00000169035 [P49862-2] |
GeneIDi | 5650 |
KEGGi | hsa:5650 |
MANE-Selecti | ENST00000595820.6; ENSP00000470538.1; NM_005046.4; NP_005037.1 |
UCSCi | uc002puo.4, human [P49862-1] |
Organism-specific databases
CTDi | 5650 |
DisGeNETi | 5650 |
GeneCardsi | KLK7 |
HGNCi | HGNC:6368, KLK7 |
HPAi | ENSG00000169035, Group enriched (esophagus, skin) |
MIMi | 604438, gene |
neXtProti | NX_P49862 |
OpenTargetsi | ENSG00000169035 |
PharmGKBi | PA30157 |
VEuPathDBi | HostDB:ENSG00000169035 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01020000230389 |
HOGENOMi | CLU_006842_1_1_1 |
InParanoidi | P49862 |
OMAi | NAWRGDS |
PhylomeDBi | P49862 |
TreeFami | TF331065 |
Enzyme and pathway databases
BRENDAi | 3.4.21.117, 2681 |
PathwayCommonsi | P49862 |
Reactomei | R-HSA-1474228, Degradation of the extracellular matrix |
SignaLinki | P49862 |
Miscellaneous databases
BioGRID-ORCSi | 5650, 6 hits in 1032 CRISPR screens |
ChiTaRSi | KLK7, human |
EvolutionaryTracei | P49862 |
GeneWikii | KLK7 |
GenomeRNAii | 5650 |
Pharosi | P49862, Tchem |
PROi | PR:P49862 |
RNActi | P49862, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169035, Expressed in mammalian vulva and 185 other tissues |
ExpressionAtlasi | P49862, baseline and differential |
Genevisiblei | P49862, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KLK7_HUMAN | |
Accessioni | P49862Primary (citable) accession number: P49862 Secondary accession number(s): A8K0U5, Q8N5N9, Q8NFV7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families