Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Stage III sporulation protein AE

Gene

spoIIIAE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required during sporulation for activation of sigma factor SpoIIIG/SigG after engulfment is completed in the prespore. Overexpression in the absence of SpoIIIJ is synthetically lethal.1 Publication

GO - Biological processi

Keywordsi

Biological processSporulation

Enzyme and pathway databases

BioCyciBSUB:BSU24390-MONOMER

Protein family/group databases

TCDBi9.B.70.1.1 the multicomponent putative spoiiiae exporter (spoiiia-e) family

Names & Taxonomyi

Protein namesi
Recommended name:
Stage III sporulation protein AE
Gene namesi
Name:spoIIIAE
Ordered Locus Names:BSU24390
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Note: Signal sequence cleavage facilitates function; replacing the signal with the first transmembrane region of SpoIVFB decreases sporulation 7-fold, whereas replacing it with the SleB signal sequence does not affect sporulation.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Transmembranei315 – 335HelicalSequence analysisAdd BLAST21
Transmembranei368 – 388HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

4000-fold reduction in sporulation efficiency.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 24MKRFQ…EIVQA → MNKWLDLILKIHVHPFLWII AALGLLTGHMK: 7-fold reduction in sporulation efficiency (first SpoIVFB transmembrane region). 1 PublicationAdd BLAST24
Mutagenesisi1 – 24Missing : 200-fold reduction in sporulation efficiency. 1 PublicationAdd BLAST24
Mutagenesisi3 – 23RFQWV…AEIVQ → SKGSIMACLILFSFTITTFI NTETIS: No change in sporulation efficiency (SleB signal sequence). 1 PublicationAdd BLAST21
Mutagenesisi24A → K: 10-fold reduction in sporulation efficiency. Sigma factor G (SigG) is not activated, signal sequence is not cleaved. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24CuratedAdd BLAST24
ChainiPRO_000007206925 – 399Stage III sporulation protein AEAdd BLAST375

Proteomic databases

PaxDbiP49782
PRIDEiP49782

Interactioni

Subunit structurei

Interacts with SpoIIIJ and YqjG.1 Publication

Protein-protein interaction databases

IntActiP49782, 2 interactors
STRINGi224308.Bsubs1_010100013376

Structurei

3D structure databases

ProteinModelPortaliP49782
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000057582
KOiK06394
OMAiEYGGFLP
PhylomeDBiP49782

Family and domain databases

InterProiView protein in InterPro
IPR014194 Spore_III_AE
PfamiView protein in Pfam
PF09546 Spore_III_AE, 1 hit
TIGRFAMsiTIGR02829 spore_III_AE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFQWVLLL AVLIIAGRAE IVQAAGNAEQ TEDHAETAEQ LAERTAASLE
60 70 80 90 100
TDKIGEFWND IMTEYGGLLP ESQKGSLMEF INGDKSFSPQ EWLKALFSYL
110 120 130 140 150
FHEVLANGKL LGTLILLTIF CVILQLLQNA FQQSTVSKVA YSIVYMVLII
160 170 180 190 200
LALNSFHVAI NYATEAIQTM TSFILALIPL LLALLASSGG AVSAAFFHPV
210 220 230 240 250
ILFLMNTSGL LIQNIVMPLI FLSAILSIVS TMTEQYKVTQ LANLLRNIAI
260 270 280 290 300
GALAVFLTIF LGVISVQGAS AAVTDGITLR TAKFITGNFI PVLGRMFTDA
310 320 330 340 350
TDTVISASLL LKNTVGILGV AILICIAAFP AIKVLSLAFI YKLAAAILQP
360 370 380 390
LGGGPVITCL DVISKSVIYI FAALAIVSLM FFLSLTVIIT AGNLTMMMK
Length:399
Mass (Da):42,921
Last modified:July 7, 2009 - v2
Checksum:i57EB1849364E9A9A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29E → G in BAA12564 (PubMed:8969508).Curated1
Sequence conflicti367 – 398VIYIF…LTMMM → GHLYFCSSRHCVSHVFFKPY CHNHSRKPHDDDEMKEAG in BAA12564 (PubMed:8969508).CuratedAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35252 Genomic DNA Translation: AAA76724.1
D84432 Genomic DNA Translation: BAA12564.1
AL009126 Genomic DNA Translation: CAB14370.2
PIRiH69711
RefSeqiNP_390319.2, NC_000964.3
WP_003230230.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14370; CAB14370; BSU24390
GeneIDi938569
KEGGibsu:BSU24390
PATRICifig|224308.179.peg.2657

Similar proteinsi

Entry informationi

Entry nameiSP3AE_BACSU
AccessioniPrimary (citable) accession number: P49782
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: May 23, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health