UniProtKB - P49718 (MCM5_MOUSE)
Protein
DNA replication licensing factor MCM5
Gene
Mcm5
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.By similarity
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 381 – 388 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: GO_Central
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- DNA replication initiation Source: GO_Central
- double-strand break repair via break-induced replication Source: GO_Central
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM5By similarity (EC:3.6.4.12)Alternative name(s): CDC46 homolog1 Publication P1-CDC46By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103197, Mcm5 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol By similarity
Other locations
- Chromosome By similarity
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Nucleus
- CMG complex Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- MCM complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000194108 | 2 – 734 | DNA replication licensing factor MCM5Add BLAST | 733 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 315 | PhosphoserineBy similarity | 1 | |
Modified residuei | 392 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 396 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 605 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P49718 |
jPOSTi | P49718 |
MaxQBi | P49718 |
PaxDbi | P49718 |
PeptideAtlasi | P49718 |
PRIDEi | P49718 |
PTM databases
iPTMneti | P49718 |
PhosphoSitePlusi | P49718 |
SwissPalmi | P49718 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000005410, Expressed in urogenital fold and 318 other tissues |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5.
Interacts with ANKRD17.
Interacts with MCMBP (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarityProtein-protein interaction databases
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P49718 |
DIPi | DIP-45875N |
IntActi | P49718, 5 interactors |
STRINGi | 10090.ENSMUSP00000126135 |
Miscellaneous databases
RNActi | P49718, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 331 – 537 | MCMAdd BLAST | 207 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 512 – 515 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0481, Eukaryota |
GeneTreei | ENSGT01010000222297 |
HOGENOMi | CLU_000995_7_2_1 |
InParanoidi | P49718 |
OMAi | PRKCTTE |
OrthoDBi | 266497at2759 |
PhylomeDBi | P49718 |
TreeFami | TF105653 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008048, MCM5 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01661, MCMPROTEIN5 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P49718-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGFDDPGIF YSDSFGGDPG AEEGQARKSQ LQRRFKEFLR QYRVGTDRTG
60 70 80 90 100
FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEELADHLHK QPAEHLQLLE
110 120 130 140 150
EAAKEVADEV TRPRPAGDEL LQDIQVMLKS DASPSSIRIL KSDMMSHLVK
160 170 180 190 200
IPGIIISASA VRAKATRISI QCRSCHNTLT NIAMRPGLEG YALPRKCNMD
210 220 230 240 250
QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ ELPDAVPHGE MPRHMQLYCD
260 270 280 290 300
RYLCDKVVPG NRVTIMGIYS IKKFGLNPSK GRDRVGVGIR SSYIRVLGIQ
310 320 330 340 350
VDTDGSGRSF AGSVSPQEEE EFRRLAALPN IYELISKSIS PSIFGGMDMK
360 370 380 390 400
KAIACLLFGG SRKRLPDGLT RRGDINLLML GDPGTAKSQL LKFVEKCSPI
410 420 430 440 450
GVYTSGKGSS AAGLTASVIR DPSSRNFIME GGAMVLADGG VVCIDEFDKM
460 470 480 490 500
REDDRVAIHE AMEQQTISIA KAGITTTLNS RCSVLAAANS VFGRWDETKG
510 520 530 540 550
EDNIDFMPTI LSRFDMIFIV KDEHNEERDM MLAKHVMTLH VSALTQTQAV
560 570 580 590 600
EGEIDLAKMK KFIAYCRARC GPRLSAEAAE KLKNRYIIMR SGARQHERDS
610 620 630 640 650
DRRSSIPITV RQLEAIVRIA EALSKMKLQP FATEADVEEA LRLFQVSTLD
660 670 680 690 700
AALSGNLSGV EGFTTQEDQE MLSRIEKQLK RRFAIGSQVS EHSIVQDFTK
710 720 730
QKYPEHAIRK VLQLMLRRGE IQHRMQRKVL YRLK
Sequence cautioni
The sequence BAA05083 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26090 mRNA Translation: BAA05083.1 Frameshift. AK133826 mRNA Translation: BAE21865.1 AC084823 Genomic DNA No translation available. CH466525 Genomic DNA Translation: EDL10828.1 CH466525 Genomic DNA Translation: EDL10827.1 CH466525 Genomic DNA Translation: EDL10829.1 BC094416 mRNA Translation: AAH94416.1 |
CCDSi | CCDS22424.1 |
PIRi | S56767 |
RefSeqi | NP_001289469.1, NM_001302540.1 NP_032592.2, NM_008566.3 |
Genome annotation databases
Ensembli | ENSMUST00000164309; ENSMUSP00000126135; ENSMUSG00000005410 ENSMUST00000212426; ENSMUSP00000148648; ENSMUSG00000005410 ENSMUST00000212811; ENSMUSP00000148525; ENSMUSG00000005410 |
GeneIDi | 17218 |
KEGGi | mmu:17218 |
UCSCi | uc009mhd.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26090 mRNA Translation: BAA05083.1 Frameshift. AK133826 mRNA Translation: BAE21865.1 AC084823 Genomic DNA No translation available. CH466525 Genomic DNA Translation: EDL10828.1 CH466525 Genomic DNA Translation: EDL10827.1 CH466525 Genomic DNA Translation: EDL10829.1 BC094416 mRNA Translation: AAH94416.1 |
CCDSi | CCDS22424.1 |
PIRi | S56767 |
RefSeqi | NP_001289469.1, NM_001302540.1 NP_032592.2, NM_008566.3 |
3D structure databases
SMRi | P49718 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P49718 |
DIPi | DIP-45875N |
IntActi | P49718, 5 interactors |
STRINGi | 10090.ENSMUSP00000126135 |
PTM databases
iPTMneti | P49718 |
PhosphoSitePlusi | P49718 |
SwissPalmi | P49718 |
Proteomic databases
EPDi | P49718 |
jPOSTi | P49718 |
MaxQBi | P49718 |
PaxDbi | P49718 |
PeptideAtlasi | P49718 |
PRIDEi | P49718 |
Protocols and materials databases
Antibodypediai | 244, 880 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000164309; ENSMUSP00000126135; ENSMUSG00000005410 ENSMUST00000212426; ENSMUSP00000148648; ENSMUSG00000005410 ENSMUST00000212811; ENSMUSP00000148525; ENSMUSG00000005410 |
GeneIDi | 17218 |
KEGGi | mmu:17218 |
UCSCi | uc009mhd.2, mouse |
Organism-specific databases
CTDi | 4174 |
MGIi | MGI:103197, Mcm5 |
Phylogenomic databases
eggNOGi | KOG0481, Eukaryota |
GeneTreei | ENSGT01010000222297 |
HOGENOMi | CLU_000995_7_2_1 |
InParanoidi | P49718 |
OMAi | PRKCTTE |
OrthoDBi | 266497at2759 |
PhylomeDBi | P49718 |
TreeFami | TF105653 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Miscellaneous databases
BioGRID-ORCSi | 17218, 7 hits in 18 CRISPR screens |
ChiTaRSi | Mcm5, mouse |
PROi | PR:P49718 |
RNActi | P49718, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005410, Expressed in urogenital fold and 318 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008048, MCM5 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01661, MCMPROTEIN5 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM5_MOUSE | |
Accessioni | P49718Primary (citable) accession number: P49718 Secondary accession number(s): Q52KC3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 8, 2019 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families