Mcm4

Functionⁱ

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.By similarity

Miscellaneous

Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.By similarity

Catalytic activityⁱ

ATP + H₂O = ADP + phosphate.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	509 – 516	ATPSequence analysis		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ATP-dependent DNA helicase activity Source: Ensembl
single-stranded DNA binding
Source: MGI
Inferred from physical interactionⁱ
- 15917436

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
DNA replication initiation Source: InterPro
DNA unwinding involved in DNA replication
Source: MGI
Inferred from physical interactionⁱ
- 15917436

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Helicase, Hydrolase
Biological process	Cell cycle, DNA replication
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-176187 Activation of ATR in response to replication stress R-MMU-68867 Assembly of the pre-replicative complex R-MMU-68949 Orc1 removal from chromatin R-MMU-68962 Activation of the pre-replicative complex R-MMU-69052 Switching of origins to a post-replicative state

Reactomeⁱ

R-MMU-176187 Activation of ATR in response to replication stress
R-MMU-68867 Assembly of the pre-replicative complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69052 Switching of origins to a post-replicative state

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA replication licensing factor MCM4 (EC:3.6.4.12) Alternative name(s): CDC21 homolog P1-CDC21
Gene namesⁱ	Name:Mcm4 Synonyms:Cdc21, Mcmd4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:103199 Mcm4

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000194102	2 – 862	DNA replication licensing factor MCM4Add BLAST		861

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineBy similarity	1
Modified residueⁱ	19	PhosphothreonineCombined sources	1
Modified residueⁱ	26	PhosphoserineBy similarity	1
Modified residueⁱ	31	PhosphoserineBy similarity	1
Modified residueⁱ	32	PhosphoserineBy similarity	1
Modified residueⁱ	101	PhosphothreonineBy similarity	1
Modified residueⁱ	104	PhosphoserineBy similarity	1
Modified residueⁱ	109	PhosphothreonineBy similarity	1
Modified residueⁱ	119	PhosphoserineBy similarity	1
Modified residueⁱ	130	PhosphoserineCombined sources	1
Modified residueⁱ	141	PhosphoserineBy similarity	1
Modified residueⁱ	144	PhosphoserineBy similarity	1
Modified residueⁱ	219	N6-acetyllysineBy similarity	1
Cross-linkⁱ	438	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	449	N6-acetyllysineBy similarity	1
Cross-linkⁱ	797	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	857	N6-acetyllysineCombined sources	1

Post-translational modificationⁱ

Sumoylated; SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro).1 Publication

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	P49717
PaxDbⁱ	P49717
PeptideAtlas More...PeptideAtlasⁱ	P49717
PRIDEⁱ	P49717

PTM databases

iPTMnetⁱ	P49717
PhosphoSitePlusⁱ	P49717
SwissPalmⁱ	P49717

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000022673
ExpressionAtlasⁱ	P49717 baseline and differential
Genevisibleⁱ	P49717 MM

Interactionⁱ

Subunit structureⁱ

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP.By similarity

Protein-protein interaction databases

BioGridⁱ	201347, 7 interactors
ComplexPortalⁱ	CPX-2941 MCM complex
CORUMⁱ	P49717
IntActⁱ	P49717, 3 interactors
Molecular INTeraction database More...MINTⁱ	P49717
STRINGⁱ	10090.ENSMUSP00000023353

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P49717
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	457 – 666	MCMAdd BLAST		210

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	641 – 644	Arginine finger		4

Sequence similaritiesⁱ

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0478 Eukaryota COG1241 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144163
HOGENOMⁱ	HOG000224127
HOVERGENⁱ	HBG102781
InParanoidⁱ	P49717
KEGG Orthology (KO) More...KOⁱ	K02212
OMAⁱ	KNTIRIC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02V8
PhylomeDBⁱ	P49717
TreeFamⁱ	TF300463

Family and domain databases

InterProⁱ	View protein in InterPro IPR031327 MCM IPR008047 MCM_4 IPR018525 MCM_CS IPR001208 MCM_dom IPR027925 MCM_N IPR033762 MCM_OB IPR012340 NA-bd_OB-fold IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR11630 PTHR11630, 1 hit PTHR11630:SF66 PTHR11630:SF66, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00493 MCM, 1 hit PF14551 MCM_N, 1 hit PF17207 MCM_OB, 1 hit
PRINTSⁱ	PR01657 MCMFAMILY PR01660 MCMPROTEIN4
SMARTⁱ	View protein in SMART SM00350 MCM, 1 hit
SUPFAMⁱ	SSF50249 SSF50249, 1 hit SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00847 MCM_1, 1 hit PS50051 MCM_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P49717-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM 
        60         70         80         90        100
PTSPGADLQS PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG 
       110        120        130        140        150
TPRSGVRGTP VRQRPDLGSA RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV 
       160        170        180        190        200
IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE ENVGIDITQP LYMQQLGEIN 
       210        220        230        240        250
ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV NEIFFDRYPD 
       260        270        280        290        300
SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE 
       310        320        330        340        350
AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM 
       360        370        380        390        400
IKLQESPEDM PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV 
       410        420        430        440        450
NPRVSNVKSV YKTHIDVIHY RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE 
       460        470        480        490        500
LSRKPDIYER LASALAPSIY EHEDIKKGIL LQLFGGTRKD FSHTGRGKFR 
       510        520        530        540        550
AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV GLTAYVMKDP 
       560        570        580        590        600
ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA 
       610        620        630        640        650
GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML 
       660        670        680        690        700
DPQDEAYDRR LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR 
       710        720        730        740        750
LSEEASQALI EAYVNMRKIG SSRGMVSAYP RQLESLIRLA EAHAKVRFSN 
       760        770        780        790        800
KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEEL 
       810        820        830        840        850
AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE EALRALADDD 
       860 
FLTVTGKTVR LL

Length:862

Mass (Da):96,736

Last modified:October 1, 1996 - v1

Checksum:ⁱ516ACC1A3C6FB16E

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	530	Q → R in AAC36509 (PubMed:9798653).Curated		1
Sequence conflictⁱ	572	I → T in AAC36509 (PubMed:9798653).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D26089 mRNA Translation: BAA05082.1 U89402 mRNA Translation: AAC36509.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27977.1
PIRⁱ	S56766
NCBI Reference Sequences More...RefSeqⁱ	NP_032591.3, NM_008565.3
UniGeneⁱ	Mm.1500

Genome annotation databases

Ensemblⁱ	ENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673
GeneIDⁱ	17217
KEGGⁱ	mmu:17217
UCSC genome browser More...UCSCⁱ	uc007yhr.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P49717	DNA helicase		MOUSE		862
Mcm4(Cdc21) protein (Fragment)		MOUSE		40

Protein	Similar proteins		Species	Score	Length
P49717	DNA replication licensing factor MCM4		HUMAN		863
UPI0003317100		SORAR		946
DNA helicase		PANPA		869
MCM4 isoform 8 (Fragment)		PANTR		212
DNA helicase		OTOGA		867
+101

Protein	Similar proteins		Species	Score	Length
P49717	DNA replication licensing factor MCM4		HUMAN		863
UPI0003317100		SORAR		946
DNA helicase		PANPA		869
MCM4 isoform 8 (Fragment)		PANTR		212
DNA helicase		OTOGA		867
+474

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D26089 mRNA Translation: BAA05082.1 U89402 mRNA Translation: AAC36509.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27977.1
PIRⁱ	S56766
NCBI Reference Sequences More...RefSeqⁱ	NP_032591.3, NM_008565.3
UniGeneⁱ	Mm.1500

3D structure databases

ProteinModelPortalⁱ	P49717
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201347, 7 interactors
ComplexPortalⁱ	CPX-2941 MCM complex
CORUMⁱ	P49717
IntActⁱ	P49717, 3 interactors
Molecular INTeraction database More...MINTⁱ	P49717
STRINGⁱ	10090.ENSMUSP00000023353

PTM databases

iPTMnetⁱ	P49717
PhosphoSitePlusⁱ	P49717
SwissPalmⁱ	P49717

Proteomic databases

EPDⁱ	P49717
PaxDbⁱ	P49717
PeptideAtlas More...PeptideAtlasⁱ	P49717
PRIDEⁱ	P49717

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673
GeneIDⁱ	17217
KEGGⁱ	mmu:17217
UCSC genome browser More...UCSCⁱ	uc007yhr.2 mouse

Organism-specific databases

CTDⁱ	4173
MGIⁱ	MGI:103199 Mcm4

Phylogenomic databases

eggNOGⁱ	KOG0478 Eukaryota COG1241 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144163
HOGENOMⁱ	HOG000224127
HOVERGENⁱ	HBG102781
InParanoidⁱ	P49717
KEGG Orthology (KO) More...KOⁱ	K02212
OMAⁱ	KNTIRIC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G02V8
PhylomeDBⁱ	P49717
TreeFamⁱ	TF300463

Enzyme and pathway databases

Reactomeⁱ	R-MMU-176187 Activation of ATR in response to replication stress R-MMU-68867 Assembly of the pre-replicative complex R-MMU-68949 Orc1 removal from chromatin R-MMU-68962 Activation of the pre-replicative complex R-MMU-69052 Switching of origins to a post-replicative state

Reactomeⁱ

R-MMU-176187 Activation of ATR in response to replication stress
R-MMU-68867 Assembly of the pre-replicative complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69052 Switching of origins to a post-replicative state

Miscellaneous databases

ChiTaRSⁱ	Mcm4 mouse
Protein Ontology More...PROⁱ	PR:P49717
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000022673
ExpressionAtlasⁱ	P49717 baseline and differential
Genevisibleⁱ	P49717 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR031327 MCM IPR008047 MCM_4 IPR018525 MCM_CS IPR001208 MCM_dom IPR027925 MCM_N IPR033762 MCM_OB IPR012340 NA-bd_OB-fold IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR11630 PTHR11630, 1 hit PTHR11630:SF66 PTHR11630:SF66, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00493 MCM, 1 hit PF14551 MCM_N, 1 hit PF17207 MCM_OB, 1 hit
PRINTSⁱ	PR01657 MCMFAMILY PR01660 MCMPROTEIN4
SMARTⁱ	View protein in SMART SM00350 MCM, 1 hit
SUPFAMⁱ	SSF50249 SSF50249, 1 hit SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00847 MCM_1, 1 hit PS50051 MCM_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MCM4_MOUSE
Accessionⁱ	P49717Primary (citable) accession number: P49717 Secondary accession number(s): O89056
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	June 20, 2018
	This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

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Supporting data

UniProtKB - P49717 (MCM4_MOUSE)

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DNA replication licensing factor MCM4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ