UniProtKB - P49717 (MCM4_MOUSE)
Protein
DNA replication licensing factor MCM4
Gene
Mcm4
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.By similarity
Miscellaneous
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.By similarity
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 509 – 516 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: GO_Central
- single-stranded DNA binding Source: MGI
GO - Biological processi
- DNA strand elongation involved in DNA replication Source: GO_Central
- DNA unwinding involved in DNA replication Source: MGI
- double-strand break repair via break-induced replication Source: GO_Central
- mitotic DNA replication initiation Source: GO_Central
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM4 (EC:3.6.4.12)Alternative name(s): CDC21 homolog P1-CDC21 |
Gene namesi | Name:Mcm4 Synonyms:Cdc21, Mcmd4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103199, Mcm4 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.By similarity
Nucleus
- CMG complex Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- MCM complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000194102 | 2 – 862 | DNA replication licensing factor MCM4Add BLAST | 861 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 19 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 31 | PhosphoserineBy similarity | 1 | |
Modified residuei | 32 | PhosphoserineBy similarity | 1 | |
Modified residuei | 101 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 104 | PhosphoserineBy similarity | 1 | |
Modified residuei | 109 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 119 | PhosphoserineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineCombined sources | 1 | |
Modified residuei | 141 | PhosphoserineBy similarity | 1 | |
Modified residuei | 144 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 438 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 449 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 797 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 857 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Sumoylated; SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro).1 Publication
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P49717 |
PaxDbi | P49717 |
PeptideAtlasi | P49717 |
PRIDEi | P49717 |
PTM databases
iPTMneti | P49717 |
PhosphoSitePlusi | P49717 |
SwissPalmi | P49717 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000022673, Expressed in cumulus cell and 305 other tissues |
ExpressionAtlasi | P49717, baseline and differential |
Genevisiblei | P49717, MM |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5.
Interacts with MCMBP (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 201347, 18 interactors |
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P49717 |
IntActi | P49717, 4 interactors |
MINTi | P49717 |
STRINGi | 10090.ENSMUSP00000023353 |
Miscellaneous databases
RNActi | P49717, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 457 – 666 | MCMAdd BLAST | 210 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 641 – 644 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0478, Eukaryota |
GeneTreei | ENSGT01010000222297 |
HOGENOMi | CLU_000995_7_1_1 |
InParanoidi | P49717 |
OMAi | YPPTRKL |
OrthoDBi | 266497at2759 |
PhylomeDBi | P49717 |
TreeFami | TF300463 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008047, MCM_4 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF66, PTHR11630:SF66, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01660, MCMPROTEIN4 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P49717-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM
60 70 80 90 100
PTSPGADLQS PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG
110 120 130 140 150
TPRSGVRGTP VRQRPDLGSA RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV
160 170 180 190 200
IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE ENVGIDITQP LYMQQLGEIN
210 220 230 240 250
ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV NEIFFDRYPD
260 270 280 290 300
SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE
310 320 330 340 350
AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM
360 370 380 390 400
IKLQESPEDM PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV
410 420 430 440 450
NPRVSNVKSV YKTHIDVIHY RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE
460 470 480 490 500
LSRKPDIYER LASALAPSIY EHEDIKKGIL LQLFGGTRKD FSHTGRGKFR
510 520 530 540 550
AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV GLTAYVMKDP
560 570 580 590 600
ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA
610 620 630 640 650
GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML
660 670 680 690 700
DPQDEAYDRR LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR
710 720 730 740 750
LSEEASQALI EAYVNMRKIG SSRGMVSAYP RQLESLIRLA EAHAKVRFSN
760 770 780 790 800
KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEEL
810 820 830 840 850
AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE EALRALADDD
860
FLTVTGKTVR LL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8VHP7 | A0A2R8VHP7_MOUSE | DNA helicase | Mcm4 | 220 | Annotation score: | ||
A0A2R8VKJ4 | A0A2R8VKJ4_MOUSE | DNA replication licensing factor MC... | Mcm4 | 86 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 530 | Q → R in AAC36509 (PubMed:9798653).Curated | 1 | |
Sequence conflicti | 572 | I → T in AAC36509 (PubMed:9798653).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26089 mRNA Translation: BAA05082.1 U89402 mRNA Translation: AAC36509.1 |
CCDSi | CCDS27977.1 |
PIRi | S56766 |
RefSeqi | NP_032591.3, NM_008565.3 |
Genome annotation databases
Ensembli | ENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673 |
GeneIDi | 17217 |
KEGGi | mmu:17217 |
UCSCi | uc007yhr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26089 mRNA Translation: BAA05082.1 U89402 mRNA Translation: AAC36509.1 |
CCDSi | CCDS27977.1 |
PIRi | S56766 |
RefSeqi | NP_032591.3, NM_008565.3 |
3D structure databases
SMRi | P49717 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201347, 18 interactors |
ComplexPortali | CPX-2941, MCM complex |
CORUMi | P49717 |
IntActi | P49717, 4 interactors |
MINTi | P49717 |
STRINGi | 10090.ENSMUSP00000023353 |
PTM databases
iPTMneti | P49717 |
PhosphoSitePlusi | P49717 |
SwissPalmi | P49717 |
Proteomic databases
EPDi | P49717 |
PaxDbi | P49717 |
PeptideAtlasi | P49717 |
PRIDEi | P49717 |
Protocols and materials databases
Antibodypediai | 1452, 428 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673 |
GeneIDi | 17217 |
KEGGi | mmu:17217 |
UCSCi | uc007yhr.2, mouse |
Organism-specific databases
CTDi | 4173 |
MGIi | MGI:103199, Mcm4 |
Phylogenomic databases
eggNOGi | KOG0478, Eukaryota |
GeneTreei | ENSGT01010000222297 |
HOGENOMi | CLU_000995_7_1_1 |
InParanoidi | P49717 |
OMAi | YPPTRKL |
OrthoDBi | 266497at2759 |
PhylomeDBi | P49717 |
TreeFami | TF300463 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex R-MMU-69052, Switching of origins to a post-replicative state |
Miscellaneous databases
BioGRID-ORCSi | 17217, 5 hits in 17 CRISPR screens |
ChiTaRSi | Mcm4, mouse |
PROi | PR:P49717 |
RNActi | P49717, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022673, Expressed in cumulus cell and 305 other tissues |
ExpressionAtlasi | P49717, baseline and differential |
Genevisiblei | P49717, MM |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008047, MCM_4 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF66, PTHR11630:SF66, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01660, MCMPROTEIN4 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM4_MOUSE | |
Accessioni | P49717Primary (citable) accession number: P49717 Secondary accession number(s): O89056 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families