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Protein

Pre-mRNA-processing factor 31

Gene

PRP31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes the association of the U4/U6.U5 tri-snRNP particle with pre-spliceosomes to form the mature spliceosomal complex.1 Publication

Miscellaneous

Present with 1260 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • spliceosomal snRNP assembly Source: SGD
  • spliceosomal tri-snRNP complex assembly Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30801-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 31
Gene namesi
Name:PRP31
Ordered Locus Names:YGR091W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR091W
SGDiS000003323 PRP31

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000585891 – 494Pre-mRNA-processing factor 31Add BLAST494

Proteomic databases

MaxQBiP49704
PaxDbiP49704
PRIDEiP49704

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei257Interaction with U4 snRNABy similarity1

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33334, 225 interactors
ComplexPortaliCPX-25 U4/U6.U5 tri-snRNP complex
CPX-32 U4/U6 snRNP
DIPiDIP-5355N
IntActiP49704, 41 interactors
MINTiP49704
STRINGi4932.YGR091W

Structurei

3D structure databases

ProteinModelPortaliP49704
SMRiP49704
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini225 – 346NopPROSITE-ProRule annotationAdd BLAST122

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili88 – 122By similarityAdd BLAST35
Coiled coili191 – 225By similarityAdd BLAST35

Domaini

Interacts with the snRNP via the Nop domain.By similarity
The coiled coil domain is formed by two non-contiguous helices.By similarity

Sequence similaritiesi

Belongs to the PRP31 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000075069
HOGENOMiHOG000000957
InParanoidiP49704
KOiK12844
OMAiKVGYDLK
OrthoDBiEOG092C4EN2

Family and domain databases

InterProiView protein in InterPro
IPR002687 Nop_dom
IPR036070 Nop_dom_sf
IPR012976 NOSIC
IPR027105 Prp31
IPR019175 Prp31_C
PANTHERiPTHR13904 PTHR13904, 1 hit
PfamiView protein in Pfam
PF01798 Nop, 1 hit
PF09785 Prp31_C, 1 hit
SMARTiView protein in SMART
SM00931 NOSIC, 1 hit
SUPFAMiSSF89124 SSF89124, 1 hit
PROSITEiView protein in PROSITE
PS51358 NOP, 1 hit

Sequencei

Sequence statusi: Complete.

P49704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES
60 70 80 90 100
IELFRTLALI SPDRLSLSET AQILPKIVDL KRILQQQEID FIKLLPFFNE
110 120 130 140 150
IIPLIKSNIK LMHNFLISLY SRRFPELSSL IPSPLQYSKV ISILENENYS
160 170 180 190 200
KNESDELFFH LENKAKLTRE QILVLTMSMK TSFKNKEPLD IKTRTQILEA
210 220 230 240 250
NSILENLWKL QEDIGQYIAS KISIIAPNVC FLVGPEIAAQ LIAHAGGVLE
260 270 280 290 300
FSRIPSCNIA SIGKNKHLSH ELHTLESGVR QEGYLFASDM IQKFPVSVHK
310 320 330 340 350
QMLRMLCAKV SLAARVDAGQ KNGDRNTVLA HKWKAELSKK ARKLSEAPSI
360 370 380 390 400
SETKALPIPE DQPKKKRAGR KFRKYKEKFR LSHVRQLQNR MEFGKQEQTV
410 420 430 440 450
LDSYGEEVGL GMSNTSLQQA VGATSGSRRS AGNQAKLTKV MKHRISEANQ
460 470 480 490
QADEFLISLG HNTEQPNLSP EMVQMHKKQH TNPEEETNWF SGHG
Length:494
Mass (Da):56,303
Last modified:February 21, 2006 - v2
Checksum:iE39AC6247859FB3B
GO

Sequence cautioni

The sequence AAA74984 differs from that shown. Reason: Frameshift at positions 361 and 394.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31L → F in AAU09731 (PubMed:9169869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31970 Genomic DNA Translation: AAA74984.1 Frameshift.
Z72876 Genomic DNA Translation: CAA97094.1
AY723814 Genomic DNA Translation: AAU09731.1
BK006941 Genomic DNA Translation: DAA08184.1
PIRiS64386
RefSeqiNP_011605.1, NM_001181220.1

Genome annotation databases

EnsemblFungiiYGR091W; YGR091W; YGR091W
GeneIDi852983
KEGGisce:YGR091W

Similar proteinsi

Entry informationi

Entry nameiPRP31_YEAST
AccessioniPrimary (citable) accession number: P49704
Secondary accession number(s): D6VUM3, Q66R99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 21, 2006
Last modified: July 18, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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