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UniProtKB - P49704 (PRP31_YEAST)
Protein
Pre-mRNA-processing factor 31
Gene
PRP31
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Promotes the association of the U4/U6.U5 tri-snRNP particle with pre-spliceosomes to form the mature spliceosomal complex.
1 PublicationMiscellaneous
Present with 1260 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- mRNA splicing, via spliceosome Source: GO_Central
- spliceosomal snRNP assembly Source: SGD
- spliceosomal tri-snRNP complex assembly Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-processing factor 31 |
Gene namesi | Name:PRP31 Ordered Locus Names:YGR091W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003323, PRP31 |
VEuPathDBi | FungiDB:YGR091W |
Subcellular locationi
Nucleus
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: ComplexPortal
- precatalytic spliceosome Source: GO_Central
- spliceosomal complex Source: ComplexPortal
- spliceosomal tri-snRNP complex Source: GO_Central
- U4 snRNP Source: GO_Central
- U4/U6 snRNP Source: ComplexPortal
- U4/U6 x U5 tri-snRNP complex Source: SGD
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058589 | 1 – 494 | Pre-mRNA-processing factor 31Add BLAST | 494 |
Proteomic databases
MaxQBi | P49704 |
PaxDbi | P49704 |
PRIDEi | P49704 |
Interactioni
Subunit structurei
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
5 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 257 | Interaction with U4 snRNABy similarity | 1 |
Protein-protein interaction databases
BioGRIDi | 33334, 229 interactors |
ComplexPortali | CPX-25, U4/U6.U5 tri-small nuclear ribonucleoprotein complex CPX-32, U4/U6 small nuclear ribonucleoprotein complex |
DIPi | DIP-5355N |
IntActi | P49704, 32 interactors |
MINTi | P49704 |
STRINGi | 4932.YGR091W |
Miscellaneous databases
RNActi | P49704, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 225 – 346 | NopPROSITE-ProRule annotationAdd BLAST | 122 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 344 – 370 | DisorderedSequence analysisAdd BLAST | 27 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 88 – 122 | By similarityAdd BLAST | 35 | |
Coiled coili | 191 – 225 | By similarityAdd BLAST | 35 |
Domaini
Interacts with the snRNP via the Nop domain.By similarity
The coiled coil domain is formed by two non-contiguous helices.By similarity
Sequence similaritiesi
Belongs to the PRP31 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2574, Eukaryota |
GeneTreei | ENSGT00550000075069 |
HOGENOMi | CLU_026337_3_0_1 |
InParanoidi | P49704 |
OMAi | IIGNGPM |
Family and domain databases
Gene3Di | 1.10.246.90, 1 hit |
InterProi | View protein in InterPro IPR042239, Nop_C IPR002687, Nop_dom IPR036070, Nop_dom_sf IPR012976, NOSIC IPR027105, Prp31 IPR019175, Prp31_C |
PANTHERi | PTHR13904, PTHR13904, 1 hit |
Pfami | View protein in Pfam PF01798, Nop, 1 hit PF09785, Prp31_C, 1 hit |
SMARTi | View protein in SMART SM00931, NOSIC, 1 hit |
SUPFAMi | SSF89124, SSF89124, 1 hit |
PROSITEi | View protein in PROSITE PS51358, NOP, 1 hit |
i Sequence
Sequence statusi: Complete.
P49704-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES
60 70 80 90 100
IELFRTLALI SPDRLSLSET AQILPKIVDL KRILQQQEID FIKLLPFFNE
110 120 130 140 150
IIPLIKSNIK LMHNFLISLY SRRFPELSSL IPSPLQYSKV ISILENENYS
160 170 180 190 200
KNESDELFFH LENKAKLTRE QILVLTMSMK TSFKNKEPLD IKTRTQILEA
210 220 230 240 250
NSILENLWKL QEDIGQYIAS KISIIAPNVC FLVGPEIAAQ LIAHAGGVLE
260 270 280 290 300
FSRIPSCNIA SIGKNKHLSH ELHTLESGVR QEGYLFASDM IQKFPVSVHK
310 320 330 340 350
QMLRMLCAKV SLAARVDAGQ KNGDRNTVLA HKWKAELSKK ARKLSEAPSI
360 370 380 390 400
SETKALPIPE DQPKKKRAGR KFRKYKEKFR LSHVRQLQNR MEFGKQEQTV
410 420 430 440 450
LDSYGEEVGL GMSNTSLQQA VGATSGSRRS AGNQAKLTKV MKHRISEANQ
460 470 480 490
QADEFLISLG HNTEQPNLSP EMVQMHKKQH TNPEEETNWF SGHG
Sequence cautioni
The sequence AAA74984 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | L → F in AAU09731 (PubMed:9169869).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U31970 Genomic DNA Translation: AAA74984.1 Frameshift. Z72876 Genomic DNA Translation: CAA97094.1 AY723814 Genomic DNA Translation: AAU09731.1 BK006941 Genomic DNA Translation: DAA08184.1 |
PIRi | S64386 |
RefSeqi | NP_011605.1, NM_001181220.1 |
Genome annotation databases
EnsemblFungii | YGR091W_mRNA; YGR091W; YGR091W |
GeneIDi | 852983 |
KEGGi | sce:YGR091W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U31970 Genomic DNA Translation: AAA74984.1 Frameshift. Z72876 Genomic DNA Translation: CAA97094.1 AY723814 Genomic DNA Translation: AAU09731.1 BK006941 Genomic DNA Translation: DAA08184.1 |
PIRi | S64386 |
RefSeqi | NP_011605.1, NM_001181220.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCM | electron microscopy | 3.80 | I | 1-494 | [»] | |
5GAN | electron microscopy | 3.60 | F | 1-494 | [»] | |
5GAP | electron microscopy | 3.60 | F | 1-494 | [»] | |
5NRL | electron microscopy | 7.20 | F | 1-494 | [»] | |
5ZWM | electron microscopy | 3.40 | L | 1-494 | [»] | |
5ZWO | electron microscopy | 3.90 | L | 1-494 | [»] | |
AlphaFoldDBi | P49704 | |||||
SMRi | P49704 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33334, 229 interactors |
ComplexPortali | CPX-25, U4/U6.U5 tri-small nuclear ribonucleoprotein complex CPX-32, U4/U6 small nuclear ribonucleoprotein complex |
DIPi | DIP-5355N |
IntActi | P49704, 32 interactors |
MINTi | P49704 |
STRINGi | 4932.YGR091W |
Proteomic databases
MaxQBi | P49704 |
PaxDbi | P49704 |
PRIDEi | P49704 |
Genome annotation databases
EnsemblFungii | YGR091W_mRNA; YGR091W; YGR091W |
GeneIDi | 852983 |
KEGGi | sce:YGR091W |
Organism-specific databases
SGDi | S000003323, PRP31 |
VEuPathDBi | FungiDB:YGR091W |
Phylogenomic databases
eggNOGi | KOG2574, Eukaryota |
GeneTreei | ENSGT00550000075069 |
HOGENOMi | CLU_026337_3_0_1 |
InParanoidi | P49704 |
OMAi | IIGNGPM |
Miscellaneous databases
PROi | PR:P49704 |
RNActi | P49704, protein |
Family and domain databases
Gene3Di | 1.10.246.90, 1 hit |
InterProi | View protein in InterPro IPR042239, Nop_C IPR002687, Nop_dom IPR036070, Nop_dom_sf IPR012976, NOSIC IPR027105, Prp31 IPR019175, Prp31_C |
PANTHERi | PTHR13904, PTHR13904, 1 hit |
Pfami | View protein in Pfam PF01798, Nop, 1 hit PF09785, Prp31_C, 1 hit |
SMARTi | View protein in SMART SM00931, NOSIC, 1 hit |
SUPFAMi | SSF89124, SSF89124, 1 hit |
PROSITEi | View protein in PROSITE PS51358, NOP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP31_YEAST | |
Accessioni | P49704Primary (citable) accession number: P49704 Secondary accession number(s): D6VUM3, Q66R99 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | February 21, 2006 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families