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Protein

Casein kinase I isoform epsilon

Gene

CSNK1E

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1 and DVL2. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

Phosphorylation leads to a decrease of the catalytic activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processBiological rhythms
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
ReactomeiR-HSA-201688 WNT mediated activation of DVL
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-400253 Circadian Clock
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-8854518 AURKA Activation by TPX2
SABIO-RKiP49674
SignaLinkiP49674
SIGNORiP49674

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform epsilon (EC:2.7.11.1)
Short name:
CKI-epsilon
Short name:
CKIe
Gene namesi
Name:CSNK1E
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000213923.10
HGNCiHGNC:2453 CSNK1E
MIMi600863 gene
neXtProtiNX_P49674

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi102800317
1454
OpenTargetsiENSG00000213923
PharmGKBiPA26953

Chemistry databases

ChEMBLiCHEMBL4937
GuidetoPHARMACOLOGYi1998

Polymorphism and mutation databases

BioMutaiCSNK1E
DMDMi1346369

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928371 – 416Casein kinase I isoform epsilonAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineCombined sources1
Modified residuei354PhosphoserineCombined sources1
Modified residuei362PhosphothreonineCombined sources1
Modified residuei363PhosphoserineCombined sources1
Modified residuei382Omega-N-methylarginineBy similarity1
Modified residuei389PhosphoserineCombined sources1
Modified residuei405PhosphoserineCombined sources1
Modified residuei408PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP49674
PaxDbiP49674
PeptideAtlasiP49674
PRIDEiP49674
ProteomicsDBi56045

PTM databases

iPTMnetiP49674
PhosphoSitePlusiP49674

Expressioni

Tissue specificityi

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.

Inductioni

Down-regulated during granulocytic differentiation.1 Publication

Gene expression databases

BgeeiENSG00000213923 Expressed in 223 organ(s), highest expression level in adenohypophysis
CleanExiHS_CSNK1E
ExpressionAtlasiP49674 baseline and differential
GenevisibleiP49674 HS

Organism-specific databases

HPAiCAB009626
HPA026288

Interactioni

Subunit structurei

Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Interacts with ANKRD6, DBNDD2, LRP5, LRP6 and SOCS3. Interacts with SNAI1 (via zinc fingers). Interacts with DDX3X; the interaction greatly improves CSNK1E affinity for ATP and enhances DVL2 phosphorylation (PubMed:23413191).7 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi107838, 182 interactors
DIPiDIP-38050N
IntActiP49674, 112 interactors
MINTiP49674
STRINGi9606.ENSP00000352929

Chemistry databases

BindingDBiP49674

Structurei

Secondary structure

1416
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP49674
SMRiP49674
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 277Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148982
HOGENOMiHOG000182055
HOVERGENiHBG000176
InParanoidiP49674
KOiK08960
OrthoDBiEOG091G0AFG
PhylomeDBiP49674
TreeFamiTF300544

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.iShow all

P49674-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH
60 70 80 90 100
IESKFYKMMQ GGVGIPSIKW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF
110 120 130 140 150
SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF
160 170 180 190 200
GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFST
260 270 280 290 300
YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGAARN
310 320 330 340 350
PEDVDRERRE HEREERMGQL RGSATRALPP GPPTGATANR LRSAAEPVAS
360 370 380 390 400
TPASRIQPAG NTSPRAISRV DRERKVSMRL HRGAPANVSS SDLTGRQEVS
410
RIPASQTSVP FDHLGK
Length:416
Mass (Da):47,315
Last modified:February 1, 1996 - v1
Checksum:iEE1B1698AE914324
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B0QY34B0QY34_HUMAN
Casein kinase I isoform epsilon
CSNK1E
336Annotation score:
B0QY36B0QY36_HUMAN
Casein kinase I isoform epsilon
CSNK1E
225Annotation score:
B0QY35B0QY35_HUMAN
Casein kinase I isoform epsilon
CSNK1E
311Annotation score:
H0Y645H0Y645_HUMAN
Casein kinase I isoform epsilon
CSNK1E
270Annotation score:
H0Y682H0Y682_HUMAN
Casein kinase I isoform epsilon
CSNK1E
144Annotation score:
H0Y5S9H0Y5S9_HUMAN
Casein kinase I isoform epsilon
CSNK1E
119Annotation score:
H7C3J2H7C3J2_HUMAN
Casein kinase I isoform epsilon
CSNK1E
118Annotation score:
H7C0Y5H7C0Y5_HUMAN
Casein kinase I isoform epsilon
CSNK1E
91Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042082256R → L in a lung adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_042083413H → R1 PublicationCorresponds to variant dbSNP:rs35665927Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37043 mRNA Translation: AAC41761.1
AB024597 mRNA Translation: BAA92345.1
AB091043 mRNA Translation: BAC10902.1
CR456429 mRNA Translation: CAG30315.1
AL020993 Genomic DNA No translation available.
BC006490 mRNA Translation: AAH06490.1
CCDSiCCDS13970.1
PIRiI61744
RefSeqiNP_001276841.1, NM_001289912.1
NP_001885.1, NM_001894.4
NP_689407.1, NM_152221.2
UniGeneiHs.474833
Hs.720223

Genome annotation databases

EnsembliENST00000359867; ENSP00000352929; ENSG00000213923
ENST00000396832; ENSP00000380044; ENSG00000213923
ENST00000403904; ENSP00000384074; ENSG00000213923
GeneIDi102800317
1454
KEGGihsa:102800317
hsa:1454

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKC1E_HUMAN
AccessioniPrimary (citable) accession number: P49674
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 12, 2018
This is version 194 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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