UniProtKB - P49655 (KCJ10_RAT)
Protein
ATP-sensitive inward rectifier potassium channel 10
Gene
Kcnj10
Organism
Rattus norvegicus (Rat)
Status
Functioni
May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. In the kidney, together with KCNJ16, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 158 | Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 210 – 217 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: RGD
- inward rectifier potassium channel activity Source: RGD
- potassium channel activity Source: RGD
- signaling receptor binding Source: RGD
GO - Biological processi
- adult walking behavior Source: RGD
- central nervous system myelination Source: RGD
- glutamate reuptake Source: RGD
- inflammatory response Source: RGD
- L-glutamate import Source: RGD
- membrane hyperpolarization Source: RGD
- oligodendrocyte development Source: RGD
- optic nerve development Source: RGD
- potassium ion homeostasis Source: RGD
- potassium ion import across plasma membrane Source: RGD
- potassium ion transmembrane transport Source: RGD
- potassium ion transport Source: RGD
- regulation of ion transmembrane transport Source: GO_Central
- regulation of long-term neuronal synaptic plasticity Source: RGD
- regulation of membrane potential Source: RGD
- regulation of resting membrane potential Source: RGD
- regulation of sensory perception of pain Source: RGD
- response to blue light Source: RGD
- response to glucocorticoid Source: RGD
- response to mineralocorticoid Source: RGD
- visual perception Source: RGD
Keywordsi
Molecular function | Ion channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | ATP-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-1296067, Potassium transport channels R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-sensitive inward rectifier potassium channel 10Alternative name(s): ATP-sensitive inward rectifier potassium channel KAB-2 BIR10 Brain-specific inwardly rectifying K(+) channel 1 Short name: BIRK1 Inward rectifier K(+) channel Kir4.1 Potassium channel, inwardly rectifying subfamily J member 10 |
Gene namesi | Name:Kcnj10 Synonyms:Kab-2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61822, Kcnj10 |
Subcellular locationi
Plasma membrane
- Basolateral cell membrane By similarity
Other locations
- Membrane By similarity; Multi-pass membrane protein By similarity
Note: In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.By similarity
Plasma Membrane
- apical plasma membrane Source: RGD
- basolateral plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- astrocyte projection Source: RGD
- cell body Source: RGD
- integral component of membrane Source: UniProtKB-KW
- microvillus Source: RGD
- presynapse Source: GOC
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 64 | CytoplasmicBy similarityAdd BLAST | 64 | |
Transmembranei | 65 – 89 | Helical; Name=M1By similarityAdd BLAST | 25 | |
Topological domaini | 90 – 114 | ExtracellularBy similarityAdd BLAST | 25 | |
Intramembranei | 115 – 126 | Helical; Pore-forming; Name=H5By similarityAdd BLAST | 12 | |
Intramembranei | 127 – 133 | Pore-formingBy similarity | 7 | |
Topological domaini | 134 – 142 | ExtracellularBy similarity | 9 | |
Transmembranei | 143 – 164 | Helical; Name=M2By similarityAdd BLAST | 22 | |
Topological domaini | 165 – 379 | CytoplasmicBy similarityAdd BLAST | 215 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154955 | 1 – 379 | ATP-sensitive inward rectifier potassium channel 10Add BLAST | 379 |
Proteomic databases
PaxDbi | P49655 |
PRIDEi | P49655 |
PTM databases
iPTMneti | P49655 |
PhosphoSitePlusi | P49655 |
Expressioni
Tissue specificityi
Predominantly expressed in the brain, including in glial cells of the cerebellum and forebrain. Expressed at lower levels in the kidney, and other peripheral tissues.2 Publications
Gene expression databases
Genevisiblei | P49655, RN |
Interactioni
Subunit structurei
Heterodimer with Kir5.1/KCNJ16; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells.
Interacts with MAGI1, alone and possibly as a heterodimer with KCNJ16; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells (By similarity).
Interacts with PATJ (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- signaling receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248335, 2 interactors |
CORUMi | P49655 |
IntActi | P49655, 3 interactors |
MINTi | P49655 |
STRINGi | 10116.ENSRNOP00000010146 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 128 – 133 | Selectivity filterBy similarity | 6 |
Sequence similaritiesi
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3827, Eukaryota |
HOGENOMi | CLU_022738_3_3_1 |
InParanoidi | P49655 |
OrthoDBi | 956263at2759 |
PhylomeDBi | P49655 |
TreeFami | TF313676 |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003269, K_chnl_inward-rec_Kir1.2 IPR013518, K_chnl_inward-rec_Kir_cyto IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit PTHR11767:SF21, PTHR11767:SF21, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit |
PIRSFi | PIRSF005465, GIRK_kir, 1 hit |
PRINTSi | PR01322, KIR12CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P49655-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL
60 70 80 90 100
YLKDLWTTFI DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG
110 120 130 140 150
PPANHTPCVV QVHTLTGAFL FSLESQTTIG YGFRYISEEC PLAIVLLIAQ
160 170 180 190 200
LVLTTILEIF ITGTFLAKIA RPKKRAETIR FSQHAVVAYH NGKLCLMIRV
210 220 230 240 250
ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV DTASDSPFLI
260 270 280 290 300
LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY
310 320 330 340 350
LPEEILWGYE FTPAISLSAS GKYVADFSLF DQVVKVASPG GLRDSTVRYG
360 370
DPEKLKLEES LREQAEKEGS ALSVRISNV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHF5 | A0A0H2UHF5_RAT | ATP-sensitive inward rectifier pota... | Kcnj10 | 378 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 73 | A → P in AAA87811 (PubMed:7624316).Curated | 1 | |
Sequence conflicti | 76 | A → T in AAA87811 (PubMed:7624316).Curated | 1 | |
Sequence conflicti | 110 – 112 | VQV → GAGA (PubMed:7624316).Curated | 3 | |
Sequence conflicti | 115 – 116 | LT → YL (PubMed:7624316).Curated | 2 | |
Sequence conflicti | 145 | V → E in AAA87811 (PubMed:7624316).Curated | 1 | |
Sequence conflicti | 190 | H → L in AAA87811 (PubMed:7624316).Curated | 1 | |
Sequence conflicti | 228 | N → D in AAA87811 (PubMed:7624316).Curated | 1 | |
Sequence conflicti | 266 | K → Q in AAA87811 (PubMed:7624316).Curated | 1 | |
Sequence conflicti | 304 | E → R in CAA58568 (PubMed:7874445).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86818 mRNA Translation: CAA60501.1 X83585 mRNA Translation: CAA58568.1 U27558 mRNA Translation: AAA87811.1 |
PIRi | A57477 |
RefSeqi | NP_113790.2, NM_031602.2 XP_008767959.1, XM_008769737.2 XP_017454263.1, XM_017598774.1 |
Genome annotation databases
GeneIDi | 29718 |
KEGGi | rno:29718 |
UCSCi | RGD:61822, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86818 mRNA Translation: CAA60501.1 X83585 mRNA Translation: CAA58568.1 U27558 mRNA Translation: AAA87811.1 |
PIRi | A57477 |
RefSeqi | NP_113790.2, NM_031602.2 XP_008767959.1, XM_008769737.2 XP_017454263.1, XM_017598774.1 |
3D structure databases
SMRi | P49655 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248335, 2 interactors |
CORUMi | P49655 |
IntActi | P49655, 3 interactors |
MINTi | P49655 |
STRINGi | 10116.ENSRNOP00000010146 |
Chemistry databases
GuidetoPHARMACOLOGYi | 438 |
PTM databases
iPTMneti | P49655 |
PhosphoSitePlusi | P49655 |
Proteomic databases
PaxDbi | P49655 |
PRIDEi | P49655 |
Genome annotation databases
GeneIDi | 29718 |
KEGGi | rno:29718 |
UCSCi | RGD:61822, rat |
Organism-specific databases
CTDi | 3766 |
RGDi | 61822, Kcnj10 |
Phylogenomic databases
eggNOGi | KOG3827, Eukaryota |
HOGENOMi | CLU_022738_3_3_1 |
InParanoidi | P49655 |
OrthoDBi | 956263at2759 |
PhylomeDBi | P49655 |
TreeFami | TF313676 |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-1296067, Potassium transport channels R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Miscellaneous databases
PROi | PR:P49655 |
Gene expression databases
Genevisiblei | P49655, RN |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003269, K_chnl_inward-rec_Kir1.2 IPR013518, K_chnl_inward-rec_Kir_cyto IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit PTHR11767:SF21, PTHR11767:SF21, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit |
PIRSFi | PIRSF005465, GIRK_kir, 1 hit |
PRINTSi | PR01322, KIR12CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCJ10_RAT | |
Accessioni | P49655Primary (citable) accession number: P49655 Secondary accession number(s): Q62790 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families