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Protein

Diacylglycerol kinase gamma

Gene

DGKG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Activity regulationi

Requires phosphatidylserine for maximal activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi188 – 1991PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi233 – 2442PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri271 – 321Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri336 – 385Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • diacylglycerol kinase activity Source: BHF-UCL
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 2681
ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis
SABIO-RKiP49619
SIGNORiP49619

Chemistry databases

SwissLipidsiSLP:000000923
SLP:000000938 [P49619-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase gamma (EC:2.7.1.107)
Short name:
DAG kinase gamma
Alternative name(s):
Diglyceride kinase gamma
Short name:
DGK-gamma
Gene namesi
Name:DGKG
Synonyms:DAGK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000058866.14
HGNCiHGNC:2853 DGKG
MIMi601854 gene
neXtProtiNX_P49619

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1608
OpenTargetsiENSG00000058866
PharmGKBiPA27314

Chemistry databases

ChEMBLiCHEMBL1075157
DrugBankiDB00144 Phosphatidyl serine

Polymorphism and mutation databases

BioMutaiDGKG
DMDMi311033457

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184591 – 791Diacylglycerol kinase gammaAdd BLAST791

Proteomic databases

EPDiP49619
PaxDbiP49619
PeptideAtlasiP49619
PRIDEiP49619
ProteomicsDBi56029
56030 [P49619-2]
56031 [P49619-3]

PTM databases

iPTMnetiP49619
PhosphoSitePlusiP49619

Expressioni

Tissue specificityi

Predominantly expressed in retina and in a much lesser extent in the brain. Other tissues contain extremely low levels of DGK-gamma.

Gene expression databases

BgeeiENSG00000058866 Expressed in 113 organ(s), highest expression level in cerebellar hemisphere
CleanExiHS_DGKG
ExpressionAtlasiP49619 baseline and differential
GenevisibleiP49619 HS

Organism-specific databases

HPAiHPA036577

Interactioni

Protein-protein interaction databases

BioGridi107978, 22 interactors
IntActiP49619, 3 interactors
STRINGi9606.ENSP00000265022

Structurei

3D structure databases

ProteinModelPortaliP49619
SMRiP49619
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 210EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini220 – 255EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini430 – 564DAGKcPROSITE-ProRule annotationAdd BLAST135

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi151 – 156Poly-Ser6

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri271 – 321Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri336 – 385Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiP49619
KOiK00901
OMAiEDGQHVW
OrthoDBiEOG091G04A5
PhylomeDBiP49619
TreeFamiTF313104

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 2 hits
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF14513 DAG_kinase_N, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P49619-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGEERWVSLT PEEFDQLQKY SEYSSKKIKD ALTEFNEGGS LKQYDPHEPI
60 70 80 90 100
SYDVFKLFMR AYLEVDLPQP LSTHLFLAFS QKPRHETSDH PTEGASNSEA
110 120 130 140 150
NSADTNIQNA DNATKADEAC APDTESNMAE KQAPAEDQVA ATPLEPPVPR
160 170 180 190 200
SSSSESPVVY LKDVVCYLSL LETGRPQDKL EFMFRLYDSD ENGLLDQAEM
210 220 230 240 250
DCIVNQMLHI AQYLEWDPTE LRPILKEMLQ GMDYDRDGFV SLQEWVHGGM
260 270 280 290 300
TTIPLLVLLG MDDSGSKGDG RHAWTMKHFK KPTYCNFCHI MLMGVRKQGL
310 320 330 340 350
CCTYCKYTVH ERCVSRNIPG CVKTYSKAKR SGEVMQHAWV EGNSSVKCDR
360 370 380 390 400
CHKSIKCYQS VTARHCVWCR MTFHRKCELS TLCDGGELRD HILLPTSICP
410 420 430 440 450
ITRDRPGEKS DGCVSAKGEL VMQYKIIPTP GTHPLLVLVN PKSGGRQGER
460 470 480 490 500
ILRKFHYLLN PKQVFNLDNG GPTPGLNFFR DTPDFRVLAC GGDGTVGWIL
510 520 530 540 550
DCIDKANFAK HPPVAVLPLG TGNDLARCLR WGGGYEGGSL TKILKDIEQS
560 570 580 590 600
PLVMLDRWHL EVIPREEVEN GDQVPYSIMN NYFSIGVDAS IAHRFHVMRE
610 620 630 640 650
KHPEKFNSRM KNKLWYFEFG TSETFAATCK KLHDHIELEC DGVGVDLSNI
660 670 680 690 700
FLEGIAILNI PSMYGGTNLW GENKKNRAVI RESRKGVTDP KELKFCVQDL
710 720 730 740 750
SDQLLEVVGL EGAMEMGQIY TGLKSAGRRL AQCASVTIRT NKLLPMQVDG
760 770 780 790
EPWMQPCCTI KITHKNQAPM MMGPPQKSSF FSLRRKSRSK D
Length:791
Mass (Da):89,124
Last modified:November 2, 2010 - v3
Checksum:iA1382CF1B2CD7362
GO
Isoform 2 (identifier: P49619-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     451-475: Missing.

Note: May be inactive.
Show »
Length:766
Mass (Da):86,303
Checksum:i1D3F8C78BD59219F
GO
Isoform 3 (identifier: P49619-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-372: Missing.

Show »
Length:752
Mass (Da):84,573
Checksum:i4B58648DC4E785ED
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C0L0H7C0L0_HUMAN
Diacylglycerol kinase gamma
DGKG
154Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271R → G in BAA05132 (PubMed:8034597).Curated1
Sequence conflicti271R → G in AAC04686 (PubMed:10071200).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020259142T → S2 PublicationsCorresponds to variant dbSNP:rs1004588Ensembl.1
Natural variantiVAR_024430316R → K4 PublicationsCorresponds to variant dbSNP:rs2193587Ensembl.1
Natural variantiVAR_020260370R → W. Corresponds to variant dbSNP:rs3213770Ensembl.1
Natural variantiVAR_036119706E → K in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039922334 – 372Missing in isoform 3. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_001267451 – 475Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26135 mRNA Translation: BAA05132.1
AF020945
, AF020922, AF020923, AF020924, AF020925, AF020926, AF020927, AF020928, AF020929, AF020930, AF020931, AF020932, AF020933, AF020934, AF020935, AF020936, AF020937, AF020938, AF020939, AF020940, AF020941, AF020942, AF020943, AF020944 Genomic DNA Translation: AAC04686.1
AK314192 mRNA Translation: BAG36871.1
AC007917 Genomic DNA No translation available.
AC112649 Genomic DNA No translation available.
BC089411 mRNA Translation: AAH89411.1
BC112363 mRNA Translation: AAI12364.1
CCDSiCCDS3274.1 [P49619-1]
CCDS43181.1 [P49619-3]
CCDS43182.1 [P49619-2]
PIRiA53691
RefSeqiNP_001074213.1, NM_001080744.1 [P49619-2]
NP_001074214.1, NM_001080745.1 [P49619-3]
NP_001337.2, NM_001346.2 [P49619-1]
UniGeneiHs.683449

Genome annotation databases

EnsembliENST00000265022; ENSP00000265022; ENSG00000058866 [P49619-1]
ENST00000344484; ENSP00000339777; ENSG00000058866 [P49619-2]
ENST00000382164; ENSP00000371599; ENSG00000058866 [P49619-3]
GeneIDi1608
KEGGihsa:1608
UCSCiuc003fqa.4 human [P49619-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26135 mRNA Translation: BAA05132.1
AF020945
, AF020922, AF020923, AF020924, AF020925, AF020926, AF020927, AF020928, AF020929, AF020930, AF020931, AF020932, AF020933, AF020934, AF020935, AF020936, AF020937, AF020938, AF020939, AF020940, AF020941, AF020942, AF020943, AF020944 Genomic DNA Translation: AAC04686.1
AK314192 mRNA Translation: BAG36871.1
AC007917 Genomic DNA No translation available.
AC112649 Genomic DNA No translation available.
BC089411 mRNA Translation: AAH89411.1
BC112363 mRNA Translation: AAI12364.1
CCDSiCCDS3274.1 [P49619-1]
CCDS43181.1 [P49619-3]
CCDS43182.1 [P49619-2]
PIRiA53691
RefSeqiNP_001074213.1, NM_001080744.1 [P49619-2]
NP_001074214.1, NM_001080745.1 [P49619-3]
NP_001337.2, NM_001346.2 [P49619-1]
UniGeneiHs.683449

3D structure databases

ProteinModelPortaliP49619
SMRiP49619
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107978, 22 interactors
IntActiP49619, 3 interactors
STRINGi9606.ENSP00000265022

Chemistry databases

ChEMBLiCHEMBL1075157
DrugBankiDB00144 Phosphatidyl serine
SwissLipidsiSLP:000000923
SLP:000000938 [P49619-1]

PTM databases

iPTMnetiP49619
PhosphoSitePlusiP49619

Polymorphism and mutation databases

BioMutaiDGKG
DMDMi311033457

Proteomic databases

EPDiP49619
PaxDbiP49619
PeptideAtlasiP49619
PRIDEiP49619
ProteomicsDBi56029
56030 [P49619-2]
56031 [P49619-3]

Protocols and materials databases

DNASUi1608
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265022; ENSP00000265022; ENSG00000058866 [P49619-1]
ENST00000344484; ENSP00000339777; ENSG00000058866 [P49619-2]
ENST00000382164; ENSP00000371599; ENSG00000058866 [P49619-3]
GeneIDi1608
KEGGihsa:1608
UCSCiuc003fqa.4 human [P49619-1]

Organism-specific databases

CTDi1608
DisGeNETi1608
EuPathDBiHostDB:ENSG00000058866.14
GeneCardsiDGKG
HGNCiHGNC:2853 DGKG
HPAiHPA036577
MIMi601854 gene
neXtProtiNX_P49619
OpenTargetsiENSG00000058866
PharmGKBiPA27314
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiP49619
KOiK00901
OMAiEDGQHVW
OrthoDBiEOG091G04A5
PhylomeDBiP49619
TreeFamiTF313104

Enzyme and pathway databases

BRENDAi2.7.1.107 2681
ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis
SABIO-RKiP49619
SIGNORiP49619

Miscellaneous databases

ChiTaRSiDGKG human
GeneWikiiDGKG
GenomeRNAii1608
PROiPR:P49619
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000058866 Expressed in 113 organ(s), highest expression level in cerebellar hemisphere
CleanExiHS_DGKG
ExpressionAtlasiP49619 baseline and differential
GenevisibleiP49619 HS

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 2 hits
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF14513 DAG_kinase_N, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDGKG_HUMAN
AccessioniPrimary (citable) accession number: P49619
Secondary accession number(s): B2RAH4, Q2M1H4, Q5FWG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 2, 2010
Last modified: November 7, 2018
This is version 179 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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