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Protein

Protein phosphatase 1F

Gene

PPM1F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi198Manganese 1By similarity1
Metal bindingi198Manganese 2By similarity1
Metal bindingi199Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi360Manganese 2By similarity1
Metal bindingi404Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processApoptosis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

SIGNORiP49593

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1F (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase
Short name:
CaM-kinase phosphatase
Short name:
CaMKPase
Partner of PIX 2
Protein fem-2 homolog
Short name:
hFem-2
Gene namesi
Name:PPM1F
Synonyms:KIAA0015, POPX2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100034.13
HGNCiHGNC:19388 PPM1F
neXtProtiNX_P49593

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9647
OpenTargetsiENSG00000100034
PharmGKBiPA134935566

Polymorphism and mutation databases

BioMutaiPPM1F
DMDMi24638458

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577581 – 454Protein phosphatase 1FAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei454PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP49593
MaxQBiP49593
PaxDbiP49593
PeptideAtlasiP49593
PRIDEiP49593
ProteomicsDBi56028

2D gel databases

OGPiP49593

PTM databases

DEPODiP49593
iPTMnetiP49593
PhosphoSitePlusiP49593

Expressioni

Gene expression databases

BgeeiENSG00000100034 Expressed in 221 organ(s), highest expression level in blood
CleanExiHS_PPM1F
ExpressionAtlasiP49593 baseline and differential
GenevisibleiP49593 HS

Organism-specific databases

HPAiHPA030989
HPA030990

Interactioni

Subunit structurei

Associates with FEM1B.

Binary interactionsi

Protein-protein interaction databases

BioGridi115005, 26 interactors
IntActiP49593, 10 interactors
MINTiP49593
STRINGi9606.ENSP00000263212

Structurei

3D structure databases

ProteinModelPortaliP49593
SMRiP49593
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 413PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST258

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi101 – 107Poly-Glu7

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00900000140907
HOGENOMiHOG000232094
HOVERGENiHBG053656
InParanoidiP49593
KOiK17502
OMAiNQLWEVC
OrthoDBiEOG091G08KG
PhylomeDBiP49593
TreeFamiTF317617

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00331 PP2C_SIG, 1 hit
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P49593-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSGAPQKSS PMASGAEETP GFLDTLLQDF PALLNPEDPL PWKAPGTVLS
60 70 80 90 100
QEEVEGELAE LAMGFLGSRK APPPLAAALA HEAVSQLLQT DLSEFRKLPR
110 120 130 140 150
EEEEEEEDDD EEEKAPVTLL DAQSLAQSFF NRLWEVAGQW QKQVPLAARA
160 170 180 190 200
SQRQWLVSIH AIRNTRRKME DRHVSLPSFN QLFGLSDPVN RAYFAVFDGH
210 220 230 240 250
GGVDAARYAA VHVHTNAARQ PELPTDPEGA LREAFRRTDQ MFLRKAKRER
260 270 280 290 300
LQSGTTGVCA LIAGATLHVA WLGDSQVILV QQGQVVKLME PHRPERQDEK
310 320 330 340 350
ARIEALGGFV SHMDCWRVNG TLAVSRAIGD VFQKPYVSGE ADAASRALTG
360 370 380 390 400
SEDYLLLACD GFFDVVPHQE VVGLVQSHLT RQQGSGLRVA EELVAAARER
410 420 430 440 450
GSHDNITVMV VFLRDPQELL EGGNQGEGDP QAEGRRQDLP SSLPEPETQA

PPRS
Length:454
Mass (Da):49,831
Last modified:November 1, 2002 - v3
Checksum:i2B49262333D4C9CF
GO
Isoform 2 (identifier: P49593-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MSSGAPQKSSPMASG → MGLCPSTRETAPPAV
     16-119: Missing.

Note: No experimental confirmation available.
Show »
Length:350
Mass (Da):38,518
Checksum:i3762283304FF2BA8
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B5MCT7B5MCT7_HUMAN
Protein phosphatase 1F (PP2C domain...
PPM1F hCG_26717
286Annotation score:
A8MX49A8MX49_HUMAN
Protein phosphatase 1F (PP2C domain...
PPM1F hCG_26717
361Annotation score:
C9J2F3C9J2F3_HUMAN
Protein phosphatase 1F
PPM1F
89Annotation score:
C9JL74C9JL74_HUMAN
Protein phosphatase 1F
PPM1F
82Annotation score:

Sequence cautioni

The sequence BAA02803 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202G → D in AAL15579 (PubMed:11559703).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050620132R → C. Corresponds to variant dbSNP:rs9610645Ensembl.1
Natural variantiVAR_036520296R → Q in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_036521417Q → K in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_024580420L → R1 PublicationCorresponds to variant dbSNP:rs2070507Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564831 – 15MSSGA…PMASG → MGLCPSTRETAPPAV in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_05648416 – 119Missing in isoform 2. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305840 mRNA Translation: AAL15579.1
AF520615 mRNA Translation: AAM76059.1
D13640 mRNA Translation: BAA02803.2 Different initiation.
AK291628 mRNA Translation: BAF84317.1
AK294557 mRNA Translation: BAH11809.1
AP000555 Genomic DNA No translation available.
D86995 Genomic DNA No translation available.
D87012 Genomic DNA No translation available.
D87019 Genomic DNA No translation available.
CCDSiCCDS13796.1 [P49593-1]
RefSeqiNP_055449.1, NM_014634.3 [P49593-1]
UniGeneiHs.112728

Genome annotation databases

EnsembliENST00000263212; ENSP00000263212; ENSG00000100034 [P49593-1]
GeneIDi9647
KEGGihsa:9647
UCSCiuc002zvp.3 human [P49593-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305840 mRNA Translation: AAL15579.1
AF520615 mRNA Translation: AAM76059.1
D13640 mRNA Translation: BAA02803.2 Different initiation.
AK291628 mRNA Translation: BAF84317.1
AK294557 mRNA Translation: BAH11809.1
AP000555 Genomic DNA No translation available.
D86995 Genomic DNA No translation available.
D87012 Genomic DNA No translation available.
D87019 Genomic DNA No translation available.
CCDSiCCDS13796.1 [P49593-1]
RefSeqiNP_055449.1, NM_014634.3 [P49593-1]
UniGeneiHs.112728

3D structure databases

ProteinModelPortaliP49593
SMRiP49593
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115005, 26 interactors
IntActiP49593, 10 interactors
MINTiP49593
STRINGi9606.ENSP00000263212

PTM databases

DEPODiP49593
iPTMnetiP49593
PhosphoSitePlusiP49593

Polymorphism and mutation databases

BioMutaiPPM1F
DMDMi24638458

2D gel databases

OGPiP49593

Proteomic databases

EPDiP49593
MaxQBiP49593
PaxDbiP49593
PeptideAtlasiP49593
PRIDEiP49593
ProteomicsDBi56028

Protocols and materials databases

DNASUi9647
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263212; ENSP00000263212; ENSG00000100034 [P49593-1]
GeneIDi9647
KEGGihsa:9647
UCSCiuc002zvp.3 human [P49593-1]

Organism-specific databases

CTDi9647
DisGeNETi9647
EuPathDBiHostDB:ENSG00000100034.13
GeneCardsiPPM1F
H-InvDBiHIX0027933
HGNCiHGNC:19388 PPM1F
HPAiHPA030989
HPA030990
neXtProtiNX_P49593
OpenTargetsiENSG00000100034
PharmGKBiPA134935566
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00900000140907
HOGENOMiHOG000232094
HOVERGENiHBG053656
InParanoidiP49593
KOiK17502
OMAiNQLWEVC
OrthoDBiEOG091G08KG
PhylomeDBiP49593
TreeFamiTF317617

Enzyme and pathway databases

SIGNORiP49593

Miscellaneous databases

ChiTaRSiPPM1F human
GeneWikiiPPM1F
GenomeRNAii9647
PROiPR:P49593

Gene expression databases

BgeeiENSG00000100034 Expressed in 221 organ(s), highest expression level in blood
CleanExiHS_PPM1F
ExpressionAtlasiP49593 baseline and differential
GenevisibleiP49593 HS

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00331 PP2C_SIG, 1 hit
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPM1F_HUMAN
AccessioniPrimary (citable) accession number: P49593
Secondary accession number(s): A8K6G3, B7Z2C3, Q96PM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 2002
Last modified: November 7, 2018
This is version 171 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
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