Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor Tu, mitochondrial

Gene

TUFM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 71GTPBy similarity8
Nucleotide bindingi126 – 130GTPBy similarity5
Nucleotide bindingi181 – 184GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB

GO - Biological processi

  • mitochondrial translational elongation Source: GO_Central
  • response to ethanol Source: Ensembl
  • translational elongation Source: UniProtKB

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5389840 Mitochondrial translation elongation
SignaLinkiP49411

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Short name:
EF-Tu
Alternative name(s):
P43
Gene namesi
Name:TUFM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000178952.8
HGNCiHGNC:12420 TUFM
MIMi602389 gene
neXtProtiNX_P49411

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Involvement in diseasei

Combined oxidative phosphorylation deficiency 4 (COXPD4)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes.
See also OMIM:610678
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031902336R → Q in COXPD4. 1 Publication1

Keywords - Diseasei

Disease mutation, Primary mitochondrial disease

Organism-specific databases

DisGeNETi7284
MalaCardsiTUFM
MIMi610678 phenotype
Orphaneti254925 Combined oxidative phosphorylation defect type 4
PharmGKBiPA37082

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

Polymorphism and mutation databases

BioMutaiTUFM
DMDMi1706611

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43Mitochondrion2 PublicationsAdd BLAST43
ChainiPRO_000000746244 – 452Elongation factor Tu, mitochondrialAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysineCombined sources1
Modified residuei88N6-acetyllysine; alternateCombined sources1
Modified residuei88N6-succinyllysine; alternateBy similarity1
Modified residuei234N6-succinyllysineBy similarity1
Modified residuei256N6-acetyllysineCombined sources1
Modified residuei278PhosphothreonineCombined sources1
Modified residuei286N6-succinyllysineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1
Modified residuei418N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP49411
MaxQBiP49411
PaxDbiP49411
PeptideAtlasiP49411
PRIDEiP49411
ProteomicsDBi56003
TopDownProteomicsiP49411

2D gel databases

DOSAC-COBS-2DPAGEiP49411
OGPiP49411
REPRODUCTION-2DPAGEiIPI00027107
SWISS-2DPAGEiP49411
UCD-2DPAGEiP49411

PTM databases

CarbonylDBiP49411
iPTMnetiP49411
PhosphoSitePlusiP49411
SwissPalmiP49411

Expressioni

Gene expression databases

BgeeiENSG00000178952 Expressed in 122 organ(s), highest expression level in testis
CleanExiHS_TUFM
ExpressionAtlasiP49411 baseline and differential
GenevisibleiP49411 HS

Organism-specific databases

HPAiCAB075730
CAB075731
HPA018991
HPA024087

Interactioni

Subunit structurei

Interacts with NLRX1 (PubMed:22749352). Interacts with ATG16L1 (PubMed:22749352).1 Publication
(Microbial infection) Interacts with human parainfluenza virus 3 matrix protein; this interaction inhibits RLR-mediated type I interferon production while promoting autophagy.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi113135, 180 interactors
CORUMiP49411
IntActiP49411, 49 interactors
MINTiP49411
STRINGi9606.ENSP00000322439

Structurei

3D structure databases

ProteinModelPortaliP49411
SMRiP49411
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 251tr-type GPROSITE-ProRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 71G1PROSITE-ProRule annotation8
Regioni105 – 109G2PROSITE-ProRule annotation5
Regioni126 – 129G3PROSITE-ProRule annotation4
Regioni181 – 184G4PROSITE-ProRule annotation4
Regioni219 – 221G5PROSITE-ProRule annotation3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0460 Eukaryota
COG0050 LUCA
HOGENOMiHOG000229290
HOVERGENiHBG001535
InParanoidiP49411
KOiK02358
OrthoDBiEOG091G0BFD
PhylomeDBiP49411
TreeFamiTF300432

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P49411-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAATLLRAT PHFSGLAAGR TFLLQGLLRL LKAPALPLLC RGLAVEAKKT
60 70 80 90 100
YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE
110 120 130 140 150
ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV
160 170 180 190 200
VAANDGPMPQ TREHLLLARQ IGVEHVVVYV NKADAVQDSE MVELVELEIR
210 220 230 240 250
ELLTEFGYKG EETPVIVGSA LCALEGRDPE LGLKSVQKLL DAVDTYIPVP
260 270 280 290 300
ARDLEKPFLL PVEAVYSVPG RGTVVTGTLE RGILKKGDEC ELLGHSKNIR
310 320 330 340 350
TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIKPHQ
360 370 380 390 400
KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRII LPPEKELAMP
410 420 430 440 450
GEDLKFNLIL RQPMILEKGQ RFTLRDGNRT IGTGLVTNTL AMTEEEKNIK

WG
Length:452
Mass (Da):49,542
Last modified:October 1, 1996 - v2
Checksum:iE37274ABFFDB5FC7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BNU3H3BNU3_HUMAN
Elongation factor Tu, mitochondrial
TUFM
101Annotation score:

Sequence cautioni

The sequence AAC60647 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH01633 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH10041 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195 – 197Missing in AAC60647 (PubMed:7828719).Curated3
Sequence conflicti384D → N in AAB00499 (PubMed:8547323).Curated1
Sequence conflicti384D → N in CAA72493 (Ref. 9) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031902336R → Q in COXPD4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38995 mRNA Translation: AAB00499.1
S75463 mRNA Translation: AAC60647.1 Different initiation.
X84694 mRNA Translation: CAA59169.1
AC133550 Genomic DNA No translation available.
BC001633 mRNA Translation: AAH01633.2 Different initiation.
BC010041 mRNA Translation: AAH10041.2 Different initiation.
Y11797 Genomic DNA Translation: CAA72493.1
PIRiS62767
S68466
RefSeqiNP_003312.3, NM_003321.4
XP_016879108.1, XM_017023619.1
UniGeneiHs.12084

Genome annotation databases

EnsembliENST00000313511; ENSP00000322439; ENSG00000178952
GeneIDi7284
KEGGihsa:7284
UCSCiuc002drh.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38995 mRNA Translation: AAB00499.1
S75463 mRNA Translation: AAC60647.1 Different initiation.
X84694 mRNA Translation: CAA59169.1
AC133550 Genomic DNA No translation available.
BC001633 mRNA Translation: AAH01633.2 Different initiation.
BC010041 mRNA Translation: AAH10041.2 Different initiation.
Y11797 Genomic DNA Translation: CAA72493.1
PIRiS62767
S68466
RefSeqiNP_003312.3, NM_003321.4
XP_016879108.1, XM_017023619.1
UniGeneiHs.12084

3D structure databases

ProteinModelPortaliP49411
SMRiP49411
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113135, 180 interactors
CORUMiP49411
IntActiP49411, 49 interactors
MINTiP49411
STRINGi9606.ENSP00000322439

Chemistry databases

DrugBankiDB04315 Guanosine-5'-Diphosphate

PTM databases

CarbonylDBiP49411
iPTMnetiP49411
PhosphoSitePlusiP49411
SwissPalmiP49411

Polymorphism and mutation databases

BioMutaiTUFM
DMDMi1706611

2D gel databases

DOSAC-COBS-2DPAGEiP49411
OGPiP49411
REPRODUCTION-2DPAGEiIPI00027107
SWISS-2DPAGEiP49411
UCD-2DPAGEiP49411

Proteomic databases

EPDiP49411
MaxQBiP49411
PaxDbiP49411
PeptideAtlasiP49411
PRIDEiP49411
ProteomicsDBi56003
TopDownProteomicsiP49411

Protocols and materials databases

DNASUi7284
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313511; ENSP00000322439; ENSG00000178952
GeneIDi7284
KEGGihsa:7284
UCSCiuc002drh.2 human

Organism-specific databases

CTDi7284
DisGeNETi7284
EuPathDBiHostDB:ENSG00000178952.8
GeneCardsiTUFM
HGNCiHGNC:12420 TUFM
HPAiCAB075730
CAB075731
HPA018991
HPA024087
MalaCardsiTUFM
MIMi602389 gene
610678 phenotype
neXtProtiNX_P49411
Orphaneti254925 Combined oxidative phosphorylation defect type 4
PharmGKBiPA37082
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0460 Eukaryota
COG0050 LUCA
HOGENOMiHOG000229290
HOVERGENiHBG001535
InParanoidiP49411
KOiK02358
OrthoDBiEOG091G0BFD
PhylomeDBiP49411
TreeFamiTF300432

Enzyme and pathway databases

ReactomeiR-HSA-5389840 Mitochondrial translation elongation
SignaLinkiP49411

Miscellaneous databases

ChiTaRSiTUFM human
GeneWikiiTUFM
GenomeRNAii7284
PROiPR:P49411
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000178952 Expressed in 122 organ(s), highest expression level in testis
CleanExiHS_TUFM
ExpressionAtlasiP49411 baseline and differential
GenevisibleiP49411 HS

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEFTU_HUMAN
AccessioniPrimary (citable) accession number: P49411
Secondary accession number(s): O15276
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 200 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again