UniProtKB - P49411 (EFTU_HUMAN)
Elongation factor Tu, mitochondrial
TUFM
Functioni
Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 64 – 71 | GTPBy similarity | 8 | |
Nucleotide bindingi | 126 – 130 | GTPBy similarity | 5 | |
Nucleotide bindingi | 181 – 184 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB
- translation elongation factor activity Source: UniProtKB
GO - Biological processi
- mitochondrial translational elongation Source: GO_Central
- response to ethanol Source: Ensembl
- translational elongation Source: UniProtKB
Keywordsi
Molecular function | Elongation factor |
Biological process | Host-virus interaction, Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P49411 |
Reactomei | R-HSA-5389840, Mitochondrial translation elongation |
SignaLinki | P49411 |
SIGNORi | P49411 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor Tu, mitochondrialShort name: EF-Tu Alternative name(s): P43 |
Gene namesi | Name:TUFM |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12420, TUFM |
MIMi | 602389, gene |
neXtProti | NX_P49411 |
VEuPathDBi | HostDB:ENSG00000178952 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrial nucleoid Source: BHF-UCL
- mitochondrion Source: UniProtKB
Other locations
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Involvement in diseasei
Combined oxidative phosphorylation deficiency 4 (COXPD4)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031902 | 336 | R → Q in COXPD4. 1 Publication | 1 |
Keywords - Diseasei
Disease variant, Primary mitochondrial diseaseOrganism-specific databases
DisGeNETi | 7284 |
MalaCardsi | TUFM |
MIMi | 610678, phenotype |
Orphaneti | 254925, Combined oxidative phosphorylation defect type 4 |
PharmGKBi | PA37082 |
Miscellaneous databases
Pharosi | P49411, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105970 |
DrugBanki | DB04315, Guanosine-5'-Diphosphate DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Genetic variation databases
BioMutai | TUFM |
DMDMi | 1706611 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 43 | Mitochondrion2 PublicationsAdd BLAST | 43 | |
ChainiPRO_0000007462 | 44 – 452 | Elongation factor Tu, mitochondrialAdd BLAST | 409 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 79 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 88 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 88 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 234 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 256 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 278 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 286 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 312 | PhosphoserineBy similarity | 1 | |
Modified residuei | 361 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 418 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P49411 |
jPOSTi | P49411 |
MassIVEi | P49411 |
MaxQBi | P49411 |
PaxDbi | P49411 |
PeptideAtlasi | P49411 |
PRIDEi | P49411 |
ProteomicsDBi | 56003 |
TopDownProteomicsi | P49411 |
2D gel databases
DOSAC-COBS-2DPAGEi | P49411 |
OGPi | P49411 |
REPRODUCTION-2DPAGEi | IPI00027107 |
SWISS-2DPAGEi | P49411 |
UCD-2DPAGEi | P49411 |
PTM databases
CarbonylDBi | P49411 |
GlyGeni | P49411, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P49411 |
MetOSitei | P49411 |
PhosphoSitePlusi | P49411 |
SwissPalmi | P49411 |
Expressioni
Gene expression databases
Bgeei | ENSG00000178952, Expressed in testis and 135 other tissues |
ExpressionAtlasi | P49411, baseline and differential |
Genevisiblei | P49411, HS |
Organism-specific databases
HPAi | ENSG00000178952, Low tissue specificity |
Interactioni
Subunit structurei
(Microbial infection) Interacts with human parainfluenza virus 3 matrix protein; this interaction inhibits RLR-mediated type I interferon production while promoting autophagy.
1 PublicationBinary interactionsi
P49411
Protein-protein interaction databases
BioGRIDi | 113135, 553 interactors |
CORUMi | P49411 |
IntActi | P49411, 116 interactors |
MINTi | P49411 |
STRINGi | 9606.ENSP00000322439 |
Miscellaneous databases
RNActi | P49411, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 251 | tr-type GPROSITE-ProRule annotationAdd BLAST | 197 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 71 | G1PROSITE-ProRule annotation | 8 | |
Regioni | 105 – 109 | G2PROSITE-ProRule annotation | 5 | |
Regioni | 126 – 129 | G3PROSITE-ProRule annotation | 4 | |
Regioni | 181 – 184 | G4PROSITE-ProRule annotation | 4 | |
Regioni | 219 – 221 | G5PROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0460, Eukaryota |
HOGENOMi | CLU_007265_0_0_1 |
InParanoidi | P49411 |
OrthoDBi | 491836at2759 |
PhylomeDBi | P49411 |
TreeFami | TF300432 |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAAATLLRAT PHFSGLAAGR TFLLQGLLRL LKAPALPLLC RGLAVEAKKT
60 70 80 90 100
YVRDKPHVNV GTIGHVDHGK TTLTAAITKI LAEGGGAKFK KYEEIDNAPE
110 120 130 140 150
ERARGITINA AHVEYSTAAR HYAHTDCPGH ADYVKNMITG TAPLDGCILV
160 170 180 190 200
VAANDGPMPQ TREHLLLARQ IGVEHVVVYV NKADAVQDSE MVELVELEIR
210 220 230 240 250
ELLTEFGYKG EETPVIVGSA LCALEGRDPE LGLKSVQKLL DAVDTYIPVP
260 270 280 290 300
ARDLEKPFLL PVEAVYSVPG RGTVVTGTLE RGILKKGDEC ELLGHSKNIR
310 320 330 340 350
TVVTGIEMFH KSLERAEAGD NLGALVRGLK REDLRRGLVM VKPGSIKPHQ
360 370 380 390 400
KVEAQVYILS KEEGGRHKPF VSHFMPVMFS LTWDMACRII LPPEKELAMP
410 420 430 440 450
GEDLKFNLIL RQPMILEKGQ RFTLRDGNRT IGTGLVTNTL AMTEEEKNIK
WG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH3BNU3 | H3BNU3_HUMAN | Elongation factor Tu, mitochondrial | TUFM | 101 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 – 197 | Missing in AAC60647 (PubMed:7828719).Curated | 3 | |
Sequence conflicti | 384 | D → N in AAB00499 (PubMed:8547323).Curated | 1 | |
Sequence conflicti | 384 | D → N in CAA72493 (Ref. 9) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031902 | 336 | R → Q in COXPD4. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38995 mRNA Translation: AAB00499.1 S75463 mRNA Translation: AAC60647.1 Different initiation. X84694 mRNA Translation: CAA59169.1 AC133550 Genomic DNA No translation available. BC001633 mRNA Translation: AAH01633.2 Different initiation. BC010041 mRNA Translation: AAH10041.2 Different initiation. Y11797 Genomic DNA Translation: CAA72493.1 |
PIRi | S62767 S68466 |
RefSeqi | NP_003312.3, NM_003321.4 XP_016879108.1, XM_017023619.1 |
Genome annotation databases
Ensembli | ENST00000313511; ENSP00000322439; ENSG00000178952 |
GeneIDi | 7284 |
KEGGi | hsa:7284 |
UCSCi | uc002drh.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38995 mRNA Translation: AAB00499.1 S75463 mRNA Translation: AAC60647.1 Different initiation. X84694 mRNA Translation: CAA59169.1 AC133550 Genomic DNA No translation available. BC001633 mRNA Translation: AAH01633.2 Different initiation. BC010041 mRNA Translation: AAH10041.2 Different initiation. Y11797 Genomic DNA Translation: CAA72493.1 |
PIRi | S62767 S68466 |
RefSeqi | NP_003312.3, NM_003321.4 XP_016879108.1, XM_017023619.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7A5G | electron microscopy | 4.33 | Z | 1-452 | [»] | |
7O9K | electron microscopy | 3.10 | t | 1-452 | [»] | |
SMRi | P49411 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113135, 553 interactors |
CORUMi | P49411 |
IntActi | P49411, 116 interactors |
MINTi | P49411 |
STRINGi | 9606.ENSP00000322439 |
Chemistry databases
ChEMBLi | CHEMBL4105970 |
DrugBanki | DB04315, Guanosine-5'-Diphosphate DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
PTM databases
CarbonylDBi | P49411 |
GlyGeni | P49411, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P49411 |
MetOSitei | P49411 |
PhosphoSitePlusi | P49411 |
SwissPalmi | P49411 |
Genetic variation databases
BioMutai | TUFM |
DMDMi | 1706611 |
2D gel databases
DOSAC-COBS-2DPAGEi | P49411 |
OGPi | P49411 |
REPRODUCTION-2DPAGEi | IPI00027107 |
SWISS-2DPAGEi | P49411 |
UCD-2DPAGEi | P49411 |
Proteomic databases
EPDi | P49411 |
jPOSTi | P49411 |
MassIVEi | P49411 |
MaxQBi | P49411 |
PaxDbi | P49411 |
PeptideAtlasi | P49411 |
PRIDEi | P49411 |
ProteomicsDBi | 56003 |
TopDownProteomicsi | P49411 |
Protocols and materials databases
ABCDi | P49411, 2 sequenced antibodies |
Antibodypediai | 13151, 317 antibodies from 30 providers |
DNASUi | 7284 |
Genome annotation databases
Ensembli | ENST00000313511; ENSP00000322439; ENSG00000178952 |
GeneIDi | 7284 |
KEGGi | hsa:7284 |
UCSCi | uc002drh.2, human |
Organism-specific databases
CTDi | 7284 |
DisGeNETi | 7284 |
GeneCardsi | TUFM |
HGNCi | HGNC:12420, TUFM |
HPAi | ENSG00000178952, Low tissue specificity |
MalaCardsi | TUFM |
MIMi | 602389, gene 610678, phenotype |
neXtProti | NX_P49411 |
Orphaneti | 254925, Combined oxidative phosphorylation defect type 4 |
PharmGKBi | PA37082 |
VEuPathDBi | HostDB:ENSG00000178952 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0460, Eukaryota |
HOGENOMi | CLU_007265_0_0_1 |
InParanoidi | P49411 |
OrthoDBi | 491836at2759 |
PhylomeDBi | P49411 |
TreeFami | TF300432 |
Enzyme and pathway databases
PathwayCommonsi | P49411 |
Reactomei | R-HSA-5389840, Mitochondrial translation elongation |
SignaLinki | P49411 |
SIGNORi | P49411 |
Miscellaneous databases
BioGRID-ORCSi | 7284, 242 hits in 1045 CRISPR screens |
ChiTaRSi | TUFM, human |
GeneWikii | TUFM |
GenomeRNAii | 7284 |
Pharosi | P49411, Tbio |
PROi | PR:P49411 |
RNActi | P49411, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000178952, Expressed in testis and 135 other tissues |
ExpressionAtlasi | P49411, baseline and differential |
Genevisiblei | P49411, HS |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EFTU_HUMAN | |
Accessioni | P49411Primary (citable) accession number: P49411 Secondary accession number(s): O15276 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 221 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families