UniProtKB - P49380 (PMA1_KLULA)
Protein
Plasma membrane ATPase
Gene
PMA1
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Functioni
The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Catalytic activityi
- EC:7.1.2.1
Activity regulationi
Activated by high pH or also by potassium ions when the medium pH is low.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 359 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 615 | MagnesiumBy similarity | 1 | |
Metal bindingi | 619 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- proton-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB-EC
GO - Biological processi
- proton export across plasma membrane Source: InterPro
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PMA1 Ordered Locus Names:KLLA0A09031g |
Organismi | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
Taxonomic identifieri | 284590 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 96 | CytoplasmicSequence analysisAdd BLAST | 96 | |
Transmembranei | 97 – 117 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 118 – 121 | ExtracellularSequence analysis | 4 | |
Transmembranei | 122 – 141 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Topological domaini | 142 – 272 | CytoplasmicSequence analysisAdd BLAST | 131 | |
Transmembranei | 273 – 294 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 295 – 305 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 306 – 328 | Helical; Name=4Sequence analysisAdd BLAST | 23 | |
Topological domaini | 329 – 700 | CytoplasmicSequence analysisAdd BLAST | 372 | |
Transmembranei | 701 – 719 | Helical; Name=5Sequence analysisAdd BLAST | 19 | |
Topological domaini | 720 – 735 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 736 – 755 | Helical; Name=6Sequence analysisAdd BLAST | 20 | |
Topological domaini | 756 – 805 | CytoplasmicSequence analysisAdd BLAST | 50 | |
Transmembranei | 806 – 826 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 827 – 838 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 839 – 855 | Helical; Name=8Sequence analysisAdd BLAST | 17 | |
Topological domaini | 856 – 899 | CytoplasmicSequence analysisAdd BLAST | 44 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 669 | M → I in 3.3; low capacity to pump out protons. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046267 | 1 – 899 | Plasma membrane ATPaseAdd BLAST | 899 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | P49380 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 566 – 571 | Poly-Gly | 6 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_0_1 |
InParanoidi | P49380 |
OMAi | FGVFCIM |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
P49380-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAATEPTKE KPVNNQDSDD EDEDIDQLIE DLQSHHGLDD ESEDDEHVAA
60 70 80 90 100
GSARPVPEEL LQTDPSYGLT SDEVTKRRKK YGLNQMSEET ENLFVKFLMF
110 120 130 140 150
FIGPIQFVME AAAILAAGLE DWVDFGVICG LLFLNAAVGF IQEYQAGSIV
160 170 180 190 200
DELKKTLANS AVVIRDGNLV EVPSNEVVPG DILQLEDGVV IPADGRLVTE
210 220 230 240 250
DCFIQIDQSA ITGESLAVDK RFGDSTFSSS TVKRGEAFMI VTATGDSTFV
260 270 280 290 300
GRAAALVNKA AAGSGHFTEV LNGIGTILLI LVIVTLLLVW VASFYRTNKI
310 320 330 340 350
VRILRYTLAI TIVGVPVGLP AVVTTTMAVG AAYLAKKQAI VQKLSAIESL
360 370 380 390 400
AGVEILCSDK TGTLTKNKLS LHEPYTVEGV DPDDLMLTAC LAASRKKKGL
410 420 430 440 450
DAIDKAFLKS LISYPRAKAA LTKYKLLEFH PFDPVSKKVT AIVESPEGER
460 470 480 490 500
IICVKGAPLF VLKTVEEEHP IPEDVRENYE NKVAELASRG FRALGVARKR
510 520 530 540 550
GEGHWEILGV MPCMDPPRDD TAQTVNEARH LGLRVKMLTG DAVGIAKETC
560 570 580 590 600
RQLGLGTNIY NAERLGLGGG GDMPGSELAD FVENADGFAE VFPQHKYNVV
610 620 630 640 650
EILQQRGYLV AMTGDGVNDA PSLKKADTGI AVEGATDAAR SAADIVFLAP
660 670 680 690 700
GLSAIIDALK TSRQIFHRMY SYVVYRIALS LHLEIFLGLW IAILNRSLNI
710 720 730 740 750
DLVVFIAIFA DVATLAIAYD NAPYSPKPVK WNLRRLWGMS VILGIILAIG
760 770 780 790 800
TWITLTTMFV PKGGIIQNFG SIDGVLFLQI SLTENWLIFI TRAAGPFWSS
810 820 830 840 850
IPSWQLSGAV LIVDIIATMF CLFGWWSQNW NDIVTVVRVW IFSFGVFCVM
860 870 880 890
GGAYYMMSES EAFDRFMNGK SRRDKPSGRS VEDFLMAMQR VSTQHEKEN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37875 Genomic DNA Translation: AAA69688.1 CR382121 Genomic DNA Translation: CAH02983.1 |
RefSeqi | XP_451395.1, XM_451395.1 |
Genome annotation databases
EnsemblFungii | CAH02983; CAH02983; KLLA0_A09031g |
GeneIDi | 2896449 |
KEGGi | kla:KLLA0_A09031g |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37875 Genomic DNA Translation: AAA69688.1 CR382121 Genomic DNA Translation: CAH02983.1 |
RefSeqi | XP_451395.1, XM_451395.1 |
3D structure databases
SMRi | P49380 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 28985.XP_451395.1 |
Proteomic databases
PRIDEi | P49380 |
Genome annotation databases
EnsemblFungii | CAH02983; CAH02983; KLLA0_A09031g |
GeneIDi | 2896449 |
KEGGi | kla:KLLA0_A09031g |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_0_1 |
InParanoidi | P49380 |
OMAi | FGVFCIM |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA1_KLULA | |
Accessioni | P49380Primary (citable) accession number: P49380 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families