UniProtKB - P49312 (ROA1_MOUSE)
Heterogeneous nuclear ribonucleoprotein A1
Hnrnpa1
Functioni
GO - Molecular functioni
- miRNA binding Source: UniProtKB
- single-stranded DNA binding Source: HGNC-UCL
GO - Biological processi
- alternative mRNA splicing, via spliceosome Source: MGI
- cellular response to sodium arsenite Source: UniProtKB
- import into nucleus Source: HGNC-UCL
- mRNA transport Source: UniProtKB-KW
- nuclear export Source: HGNC-UCL
- RNA splicing Source: MGI
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing, mRNA transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-6803529, FGFR2 alternative splicing R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA |
Names & Taxonomyi
Protein namesi | Recommended name: Heterogeneous nuclear ribonucleoprotein A1Short name: hnRNP A1 Alternative name(s): HDP-1 Helix-destabilizing protein Single-strand-binding protein Topoisomerase-inhibitor suppressed hnRNP core protein A1 Cleaved into the following chain: |
Gene namesi | Name:Hnrnpa1 Synonyms:Fli-2, Hnrpa1, Tis |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104820, Hnrnpa1 |
Subcellular locationi
Nucleus
- nucleoplasm Source: HGNC-UCL
- nucleus Source: MGI
- spliceosomal complex Source: HGNC-UCL
Other locations
- cytoplasm Source: HGNC-UCL
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424511 | 1 – 320 | Heterogeneous nuclear ribonucleoprotein A1Add BLAST | 320 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000081830 | 2 – 320 | Heterogeneous nuclear ribonucleoprotein A1, N-terminally processedAdd BLAST | 319 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylserine; in Heterogeneous nuclear ribonucleoprotein A1, N-terminally processedBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 3 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 3 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 4 | PhosphoserineBy similarity | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Cross-linki | 8 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 22 | PhosphoserineCombined sources | 1 | |
Cross-linki | 78 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 113 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 179 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 183 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 192 | Phosphoserine; by MKNK2By similarity | 1 | |
Modified residuei | 194 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 194 | Dimethylated arginine; alternateBy similarity | 1 | |
Modified residuei | 194 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 206 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 206 | Dimethylated arginine; alternateBy similarity | 1 | |
Modified residuei | 206 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 218 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 218 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 225 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 225 | Dimethylated arginine; alternateBy similarity | 1 | |
Modified residuei | 225 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 232 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 232 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 284 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 285 | PhosphoserineBy similarity | 1 | |
Modified residuei | 298 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 298 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 300 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 309 | PhosphoserineBy similarity | 1 | |
Modified residuei | 310 | Phosphoserine; by MKNK2By similarity | 1 | |
Modified residuei | 311 | Phosphoserine; by MKNK2By similarity | 1 | |
Modified residuei | 312 | Phosphoserine; by MKNK2By similarity | 1 | |
Modified residuei | 313 | PhosphoserineBy similarity | 1 | |
Modified residuei | 316 | PhosphoserineBy similarity | 1 | |
Modified residuei | 318 | Omega-N-methylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P49312 |
jPOSTi | P49312 |
MaxQBi | P49312 |
PaxDbi | P49312 |
PeptideAtlasi | P49312 |
PRIDEi | P49312 |
TopDownProteomicsi | P49312-1 [P49312-1] |
PTM databases
iPTMneti | P49312 |
PhosphoSitePlusi | P49312 |
SwissPalmi | P49312 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000046434, Expressed in ganglionic eminence and 116 other tissues |
ExpressionAtlasi | P49312, baseline and differential |
Genevisiblei | P49312, MM |
Interactioni
Subunit structurei
Identified in the spliceosome C complex.
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.
Interacts with SEPT6.
Interacts with C9orf72.
Interacts with KHDRBS1.
Interacts with UBQLN2 (By similarity).
Interacts with PPIA/CYPA (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 200357, 13 interactors |
IntActi | P49312, 12 interactors |
MINTi | P49312 |
Miscellaneous databases
RNActi | P49312, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 97 | RRM 1PROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 105 – 184 | RRM 2PROSITE-ProRule annotationAdd BLAST | 80 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 4 – 94 | Globular A domainAdd BLAST | 91 | |
Regioni | 95 – 185 | Globular B domainAdd BLAST | 91 | |
Regioni | 218 – 240 | RNA-binding RGG-boxAdd BLAST | 23 | |
Regioni | 268 – 305 | Nuclear targeting sequenceBy similarityAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 195 – 320 | Gly-richAdd BLAST | 126 | |
Compositional biasi | 308 – 313 | Poly-Ser | 6 |
Keywords - Domaini
RepeatPhylogenomic databases
GeneTreei | ENSGT00950000183123 |
HOGENOMi | CLU_012062_1_0_1 |
InParanoidi | P49312 |
Family and domain databases
CDDi | cd12761, RRM1_hnRNPA1, 1 hit cd12580, RRM2_hnRNPA1, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR021662, HnRNPA1 IPR034845, hnRNPA1_RRM1 IPR034803, hnRNPA1_RRM2 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF11627, HnRNPA1, 1 hit PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSKSESPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN
60 70 80 90 100
TKRSRGFGFV TYATVEEVDA AMNARPHKVD GRVVEPKRAV SREDSQRPGA
110 120 130 140 150
HLTVKKIFVG GIKEDTEEHH LRDYFEQYGK IEVIEIMTDR GSGKKRGFAF
160 170 180 190 200
VTFDDHDSVD KIVIQKYHTV NGHNCEVRKA LSKQEMASAS SSQRGRSGSG
210 220 230 240 250
NFGGGRGGGF GGNDNFGRGG NFSGRGGFGG SRGGGGYGGS GDGYNGFGND
260 270 280 290 300
GSNFGGGGSY NDFGNYNNQS SNFGPMKGGN FGGRSSGPYG GGGQYFAKPR
310 320
NQGGYGGSSS SSSYGSGRRF
The sequence of this isoform differs from the canonical sequence as follows:
252-303: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5EBP8 | Q5EBP8_MOUSE | Helix-destabilizing protein | Hnrnpa1 Hnrpa1 | 373 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005825 | 252 – 303 | Missing in isoform Short. CuratedAdd BLAST | 52 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M99167 mRNA Translation: AAA37633.1 D86729 mRNA Translation: BAA13162.1 D86728 mRNA Translation: BAA13161.1 AK007802 mRNA Translation: BAB25267.1 AK088308 mRNA Translation: BAC40273.1 AK131999 mRNA Translation: BAE20929.1 AK135391 mRNA Translation: BAE22518.1 AK161730 mRNA Translation: BAE36552.1 AK166901 mRNA Translation: BAE39104.1 AK167161 mRNA Translation: BAE39302.1 AK167810 mRNA Translation: BAE39837.1 AK167913 mRNA Translation: BAE39920.1 AK168420 mRNA Translation: BAE40333.1 AK168531 mRNA Translation: BAE40409.1 BC080675 mRNA Translation: AAH80675.1 BC083136 mRNA Translation: AAH83136.1 |
CCDSi | CCDS37233.1 [P49312-1] |
PIRi | A44485 |
RefSeqi | NP_034577.1, NM_010447.5 [P49312-1] |
Genome annotation databases
Ensembli | ENSMUST00000087351; ENSMUSP00000084609; ENSMUSG00000046434 [P49312-1] ENSMUST00000230171; ENSMUSP00000155833; ENSMUSG00000046434 [P49312-1] ENSMUST00000231141; ENSMUSP00000155311; ENSMUSG00000046434 [P49312-2] |
GeneIDi | 15382 |
KEGGi | mmu:15382 |
UCSCi | uc007xxp.2, mouse [P49312-1] uc011zrp.1, mouse [P49312-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M99167 mRNA Translation: AAA37633.1 D86729 mRNA Translation: BAA13162.1 D86728 mRNA Translation: BAA13161.1 AK007802 mRNA Translation: BAB25267.1 AK088308 mRNA Translation: BAC40273.1 AK131999 mRNA Translation: BAE20929.1 AK135391 mRNA Translation: BAE22518.1 AK161730 mRNA Translation: BAE36552.1 AK166901 mRNA Translation: BAE39104.1 AK167161 mRNA Translation: BAE39302.1 AK167810 mRNA Translation: BAE39837.1 AK167913 mRNA Translation: BAE39920.1 AK168420 mRNA Translation: BAE40333.1 AK168531 mRNA Translation: BAE40409.1 BC080675 mRNA Translation: AAH80675.1 BC083136 mRNA Translation: AAH83136.1 |
CCDSi | CCDS37233.1 [P49312-1] |
PIRi | A44485 |
RefSeqi | NP_034577.1, NM_010447.5 [P49312-1] |
3D structure databases
BMRBi | P49312 |
SMRi | P49312 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200357, 13 interactors |
IntActi | P49312, 12 interactors |
MINTi | P49312 |
PTM databases
iPTMneti | P49312 |
PhosphoSitePlusi | P49312 |
SwissPalmi | P49312 |
Proteomic databases
EPDi | P49312 |
jPOSTi | P49312 |
MaxQBi | P49312 |
PaxDbi | P49312 |
PeptideAtlasi | P49312 |
PRIDEi | P49312 |
TopDownProteomicsi | P49312-1 [P49312-1] |
Protocols and materials databases
Antibodypediai | 7969, 490 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000087351; ENSMUSP00000084609; ENSMUSG00000046434 [P49312-1] ENSMUST00000230171; ENSMUSP00000155833; ENSMUSG00000046434 [P49312-1] ENSMUST00000231141; ENSMUSP00000155311; ENSMUSG00000046434 [P49312-2] |
GeneIDi | 15382 |
KEGGi | mmu:15382 |
UCSCi | uc007xxp.2, mouse [P49312-1] uc011zrp.1, mouse [P49312-2] |
Organism-specific databases
CTDi | 3178 |
MGIi | MGI:104820, Hnrnpa1 |
Phylogenomic databases
GeneTreei | ENSGT00950000183123 |
HOGENOMi | CLU_012062_1_0_1 |
InParanoidi | P49312 |
Enzyme and pathway databases
Reactomei | R-MMU-6803529, FGFR2 alternative splicing R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA |
Miscellaneous databases
BioGRID-ORCSi | 15382, 4 hits in 16 CRISPR screens |
ChiTaRSi | Hnrnpa1, mouse |
PROi | PR:P49312 |
RNActi | P49312, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046434, Expressed in ganglionic eminence and 116 other tissues |
ExpressionAtlasi | P49312, baseline and differential |
Genevisiblei | P49312, MM |
Family and domain databases
CDDi | cd12761, RRM1_hnRNPA1, 1 hit cd12580, RRM2_hnRNPA1, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR021662, HnRNPA1 IPR034845, hnRNPA1_RRM1 IPR034803, hnRNPA1_RRM2 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF11627, HnRNPA1, 1 hit PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ROA1_MOUSE | |
Accessioni | P49312Primary (citable) accession number: P49312 Secondary accession number(s): P97312, Q3V269 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot