UniProtKB - P49282 (NRAM2_MOUSE)
Natural resistance-associated macrophage protein 2
Slc11a2
Functioni
Miscellaneous
GO - Molecular functioni
- cadmium ion binding Source: UniProtKB
- cadmium ion transmembrane transporter activity Source: MGI
- cobalt ion binding Source: MGI
- cobalt ion transmembrane transporter activity Source: MGI
- copper ion binding Source: MGI
- copper ion transmembrane transporter activity Source: MGI
- ferrous iron transmembrane transporter activity Source: MGI
- inorganic cation transmembrane transporter activity Source: MGI
- iron ion binding Source: MGI
- iron ion transmembrane transporter activity Source: MGI
- lead ion transmembrane transporter activity Source: MGI
- manganese ion binding Source: MGI
- manganese ion transmembrane transporter activity Source: MGI
- nickel cation binding Source: MGI
- nickel cation transmembrane transporter activity Source: MGI
- proton transmembrane transporter activity Source: MGI
- retromer complex binding Source: MGI
- solute:proton symporter activity Source: MGI
- transition metal ion transmembrane transporter activity Source: MGI
- vanadium ion transmembrane transporter activity Source: MGI
- zinc ion binding Source: MGI
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- cadmium ion transmembrane transport Source: UniProtKB
- cellular response to oxidative stress Source: MGI
- cobalt ion transport Source: MGI
- copper ion import across plasma membrane Source: MGI
- copper ion transport Source: MGI
- dendrite morphogenesis Source: MGI
- erythrocyte development Source: MGI
- heme biosynthetic process Source: MGI
- iron import into cell Source: MGI
- iron ion transmembrane transport Source: MGI
- iron ion transport Source: MGI
- lead ion transport Source: MGI
- learning or memory Source: MGI
- manganese ion transport Source: MGI
- multicellular organismal iron ion homeostasis Source: MGI
- nickel cation transport Source: MGI
- porphyrin-containing compound biosynthetic process Source: MGI
- porphyrin-containing compound metabolic process Source: MGI
- proton transmembrane transport Source: MGI
- response to hypoxia Source: GO_Central
- response to iron ion Source: Ensembl
- response to manganese ion Source: MGI
- transition metal ion transport Source: MGI
- vanadium ion transport Source: MGI
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron |
Enzyme and pathway databases
Reactomei | R-MMU-425410, Metal ion SLC transporters R-MMU-917937, Iron uptake and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Natural resistance-associated macrophage protein 2Short name: NRAMP 2 Alternative name(s): Divalent cation transporter 1 Divalent metal transporter 1 Short name: DMT-1 Solute carrier family 11 member 2 |
Gene namesi | Name:Slc11a2 Synonyms:Dct1, Dmt1, Nramp2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1345279, Slc11a2 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein By similarity
Mitochondrion
- Mitochondrion outer membrane By similarity; Multi-pass membrane protein By similarity
Endosome
- Endosome membrane By similarity; Multi-pass membrane protein By similarity
Note: Also found in extracellular vesicles different from exosomes.1 Publication
Endosome
- early endosome Source: MGI
- endosome Source: MGI
- late endosome Source: MGI
- late endosome membrane Source: MGI
- recycling endosome Source: MGI
Extracellular region or secreted
- extracellular vesicle Source: UniProtKB
Golgi apparatus
- trans-Golgi network Source: MGI
Lysosome
- lysosomal membrane Source: MGI
- lysosome Source: MGI
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB-SubCell
- mitochondrion Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- brush border membrane Source: BHF-UCL
- integral component of plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- apical part of cell Source: MGI
- basal part of cell Source: MGI
- brush border Source: MGI
- cell surface Source: MGI
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
- endomembrane system Source: MGI
- integral component of membrane Source: MGI
- paraferritin complex Source: MGI
- perinuclear region of cytoplasm Source: MGI
- vacuole Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 69 | CytoplasmicSequence analysisAdd BLAST | 69 | |
Transmembranei | 70 – 90 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 91 – 95 | ExtracellularSequence analysis | 5 | |
Transmembranei | 96 – 117 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 118 – 154 | CytoplasmicSequence analysisAdd BLAST | 37 | |
Transmembranei | 155 – 175 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 176 – 179 | ExtracellularSequence analysis | 4 | |
Transmembranei | 180 – 194 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 195 – 208 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 230 – 255 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 256 – 276 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 277 – 301 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 302 – 322 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 323 – 360 | ExtracellularSequence analysisAdd BLAST | 38 | |
Transmembranei | 361 – 381 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 382 – 408 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 409 – 429 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 430 – 440 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 441 – 461 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 462 – 482 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 483 – 503 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 504 – 506 | ExtracellularSequence analysis | 3 | |
Transmembranei | 507 – 527 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 528 – 568 | CytoplasmicSequence analysisAdd BLAST | 41 |
Keywords - Cellular componenti
Cell membrane, Endosome, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Involvement in diseasei
Disruption phenotypei
Keywords - Diseasei
Disease mutationPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212595 | 1 – 568 | Natural resistance-associated macrophage protein 2Add BLAST | 568 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Glycosylationi | 336 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Glycosylationi | 349 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Modified residuei | 564 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 567 | PhosphoserineCombined sources | 1 | ||
Isoform 1 (identifier: P49282-2) | |||||
Modified residuei | 556 | PhosphoserineCombined sources | 1 | ||
Isoform 3 (identifier: P49282-3) | |||||
Modified residuei | 586 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P49282 |
jPOSTi | P49282 |
MaxQBi | P49282 |
PaxDbi | P49282 |
PeptideAtlasi | P49282 |
PRIDEi | P49282 |
PTM databases
GlyGeni | P49282, 2 sites |
iPTMneti | P49282 |
PhosphoSitePlusi | P49282 |
SwissPalmi | P49282 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000023030, Expressed in cardiac ventricle and 283 other tissues |
ExpressionAtlasi | P49282, baseline and differential |
Genevisiblei | P49282, MM |
Interactioni
Subunit structurei
Forms a complex with NDFIP1 and NEDD4L, in cortical neurons, in response to iron and colbalt exposure; this interaction leads to ubiquitination by NEDD4L and proteasome-dependent degradation.
Interacts with NDFIP2.
Interacts with COX2 and TOM6 at the outer mitochondrion membrane (By similarity).
Interacts with ARRDC1; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism (By similarity).
Interacts with ARRDC4; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism (PubMed:27462458).
By similarity1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000023774 |
Miscellaneous databases
RNActi | P49282, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1291, Eukaryota |
GeneTreei | ENSGT00940000155330 |
HOGENOMi | CLU_020088_5_2_1 |
InParanoidi | P49282 |
OMAi | LIQYQSA |
OrthoDBi | 666470at2759 |
PhylomeDBi | P49282 |
TreeFami | TF315185 |
Family and domain databases
HAMAPi | MF_00221, NRAMP, 1 hit |
InterProi | View protein in InterPro IPR001046, NRAMP_fam |
PANTHERi | PTHR11706, PTHR11706, 1 hit |
Pfami | View protein in Pfam PF01566, Nramp, 1 hit |
PRINTSi | PR00447, NATRESASSCMP |
TIGRFAMsi | TIGR01197, nramp, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVLDPKEKMP DDGASGDHGD SASLGAINPA YSNSSLPHST GDSEEPFTTY
60 70 80 90 100
FDEKIPIPEE EYSCFSFRKL WAFTGPGFLM SIAYLDPGNI ESDLQSGAVA
110 120 130 140 150
GFKLLWVLLL ATIVGLLLQR LAARLGVVTG LHLAEVCHRQ YPKVPRIILW
160 170 180 190 200
LMVELAIIGS DMQEVIGSAI AINLLSAGRV PLWGGVLITI ADTFVFLFLD
210 220 230 240 250
KYGLRKLEAF FGFLITIMAL TFGYEYITVK PSQSQVLRGM FVPSCPGCRT
260 270 280 290 300
PQVEQAVGIV GAVIMPHNMY LHSALVKSRQ VNRANKQEVR EANKYFFIES
310 320 330 340 350
CIALFVSFII NVFVVSVFAE AFFEKTNKQV VEVCKNNSSP HADLFPSDNS
360 370 380 390 400
TLAVDIYKGG VVLGCYFGPA ALYIWAVGIL AAGQSSTMTG TYSGQFVMEG
410 420 430 440 450
FLNLKWSRFA RVILTRSIAI IPTLLVAVFQ DVEHLTGMND FLNVLQSLQL
460 470 480 490 500
PFALIPILTF TSLRPVMSEF SNGIGWRIAG GILVLIVCSI NMYFVVVYVQ
510 520 530 540 550
ELGHVALYVV AAVVSVAYLT FVFYLGWQCL IALGLSFLDC GRSYRLGLTA
560
QPELYLLNTV DADSVVSR
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z314 | D3Z314_MOUSE | Natural resistance-associated macro... | Slc11a2 | 170 | Annotation score: | ||
F6ZM31 | F6ZM31_MOUSE | Natural resistance-associated macro... | Slc11a2 | 220 | Annotation score: | ||
D3Z0R6 | D3Z0R6_MOUSE | Natural resistance-associated macro... | Slc11a2 | 110 | Annotation score: | ||
D3YXP3 | D3YXP3_MOUSE | Natural resistance-associated macro... | Slc11a2 | 50 | Annotation score: | ||
D6RGP0 | D6RGP0_MOUSE | Natural resistance-associated macro... | Slc11a2 | 68 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | K → E in BAE28454 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 6 | K → E in CAD38518 (PubMed:12209011).Curated | 1 | |
Sequence conflicti | 68 | R → S in AAC24496 (Ref. 2) Curated | 1 | |
Sequence conflicti | 69 – 70 | Missing in CAD38518 (PubMed:12209011).Curated | 2 | |
Sequence conflicti | 142 | P → R in BAC38930 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 182 | L → V in AAC42051 (PubMed:7789986).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 185 | G → R in microcytic anemia. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038145 | 1 | M → MGKKQPRAAAAAPNCELKSY SKSTDPQVSTM in isoform 3 and isoform 4. Curated | 1 | |
Alternative sequenceiVSP_003596 | 544 – 568 | YRLGL…SVVSR → VSISKVLLSEDTSGGNIK in isoform 1 and isoform 3. 2 PublicationsAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33415 mRNA Translation: AAC42051.1 AF029758 mRNA Translation: AAC24496.1 AK049856 mRNA Translation: BAC33960.1 AK083478 mRNA Translation: BAC38930.1 AK148276 mRNA Translation: BAE28454.1 CH466550 Genomic DNA Translation: EDL04090.1 BC019137 mRNA Translation: AAH19137.1 AJ493663 mRNA Translation: CAD38518.1 Frameshift. |
CCDSi | CCDS37211.1 [P49282-1] CCDS49733.1 [P49282-2] |
PIRi | A56852 |
RefSeqi | NP_001139633.1, NM_001146161.1 [P49282-2] NP_032758.2, NM_008732.2 [P49282-1] XP_006520640.1, XM_006520577.3 [P49282-1] XP_006520641.1, XM_006520578.3 [P49282-1] XP_011243789.1, XM_011245487.2 [P49282-4] |
Genome annotation databases
Ensembli | ENSMUST00000023774; ENSMUSP00000023774; ENSMUSG00000023030 [P49282-1] ENSMUST00000138843; ENSMUSP00000116463; ENSMUSG00000023030 [P49282-2] |
GeneIDi | 18174 |
KEGGi | mmu:18174 |
UCSCi | uc007xrc.2, mouse [P49282-1] uc007xrd.2, mouse [P49282-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33415 mRNA Translation: AAC42051.1 AF029758 mRNA Translation: AAC24496.1 AK049856 mRNA Translation: BAC33960.1 AK083478 mRNA Translation: BAC38930.1 AK148276 mRNA Translation: BAE28454.1 CH466550 Genomic DNA Translation: EDL04090.1 BC019137 mRNA Translation: AAH19137.1 AJ493663 mRNA Translation: CAD38518.1 Frameshift. |
CCDSi | CCDS37211.1 [P49282-1] CCDS49733.1 [P49282-2] |
PIRi | A56852 |
RefSeqi | NP_001139633.1, NM_001146161.1 [P49282-2] NP_032758.2, NM_008732.2 [P49282-1] XP_006520640.1, XM_006520577.3 [P49282-1] XP_006520641.1, XM_006520578.3 [P49282-1] XP_011243789.1, XM_011245487.2 [P49282-4] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000023774 |
PTM databases
GlyGeni | P49282, 2 sites |
iPTMneti | P49282 |
PhosphoSitePlusi | P49282 |
SwissPalmi | P49282 |
Proteomic databases
EPDi | P49282 |
jPOSTi | P49282 |
MaxQBi | P49282 |
PaxDbi | P49282 |
PeptideAtlasi | P49282 |
PRIDEi | P49282 |
Protocols and materials databases
Antibodypediai | 26282, 357 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023774; ENSMUSP00000023774; ENSMUSG00000023030 [P49282-1] ENSMUST00000138843; ENSMUSP00000116463; ENSMUSG00000023030 [P49282-2] |
GeneIDi | 18174 |
KEGGi | mmu:18174 |
UCSCi | uc007xrc.2, mouse [P49282-1] uc007xrd.2, mouse [P49282-2] |
Organism-specific databases
CTDi | 4891 |
MGIi | MGI:1345279, Slc11a2 |
Phylogenomic databases
eggNOGi | KOG1291, Eukaryota |
GeneTreei | ENSGT00940000155330 |
HOGENOMi | CLU_020088_5_2_1 |
InParanoidi | P49282 |
OMAi | LIQYQSA |
OrthoDBi | 666470at2759 |
PhylomeDBi | P49282 |
TreeFami | TF315185 |
Enzyme and pathway databases
Reactomei | R-MMU-425410, Metal ion SLC transporters R-MMU-917937, Iron uptake and transport |
Miscellaneous databases
BioGRID-ORCSi | 18174, 0 hits in 17 CRISPR screens |
ChiTaRSi | Slc11a2, mouse |
PROi | PR:P49282 |
RNActi | P49282, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023030, Expressed in cardiac ventricle and 283 other tissues |
ExpressionAtlasi | P49282, baseline and differential |
Genevisiblei | P49282, MM |
Family and domain databases
HAMAPi | MF_00221, NRAMP, 1 hit |
InterProi | View protein in InterPro IPR001046, NRAMP_fam |
PANTHERi | PTHR11706, PTHR11706, 1 hit |
Pfami | View protein in Pfam PF01566, Nramp, 1 hit |
PRINTSi | PR00447, NATRESASSCMP |
TIGRFAMsi | TIGR01197, nramp, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NRAM2_MOUSE | |
Accessioni | P49282Primary (citable) accession number: P49282 Secondary accession number(s): O54903 Q8VCU6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | September 22, 2009 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families