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Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Calcium 1By similarity1
Metal bindingi40Calcium 2By similarity1
Metal bindingi41Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi43Calcium 1By similarity1
Metal bindingi43Calcium 2By similarity1
Metal bindingi65Calcium 1By similarity1
Metal bindingi93Calcium 2By similarity1
Metal bindingi94Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi95Calcium 2By similarity1
Active sitei228NucleophileBy similarity1
Active sitei549Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-111995 phospho-PLA2 pathway
R-GGA-1482788 Acyl chain remodelling of PC
R-GGA-1482798 Acyl chain remodeling of CL
R-GGA-1482801 Acyl chain remodelling of PS
R-GGA-1482839 Acyl chain remodelling of PE
R-GGA-1482922 Acyl chain remodelling of PI
R-GGA-1482925 Acyl chain remodelling of PG
R-GGA-1483115 Hydrolysis of LPC
R-GGA-1483166 Synthesis of PA
R-GGA-2142753 Arachidonic acid metabolism
R-GGA-418592 ADP signalling through P2Y purinoceptor 1
R-GGA-432142 Platelet sensitization by LDL
R-GGA-6811436 COPI-independent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 8

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872661 – 748Cytosolic phospholipase A2Add BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei505Phosphoserine; by MAPKBy similarity1

Post-translational modificationi

Activated by phosphorylation on a serine residue.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49147

Expressioni

Gene expression databases

BgeeiENSGALG00000005065

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517

Structurei

3D structure databases

ProteinModelPortaliP49147
SMRiP49147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 106C2PROSITE-ProRule annotationAdd BLAST102
Domaini138 – 740PLA2cPROSITE-ProRule annotationAdd BLAST603

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 178Phospholipid bindingAdd BLAST178

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG1012 Eukaryota
KOG1325 Eukaryota
ENOG410XR72 LUCA
GeneTreeiENSGT00550000074489
HOGENOMiHOG000115420
HOVERGENiHBG053479
InParanoidiP49147
KOiK16342
OMAiRFSMALC
OrthoDBiEOG091G0276
PhylomeDBiP49147

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF01735 PLA2_B, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS51210 PLA2C, 1 hit

Sequencei

Sequence statusi: Complete.

P49147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI VVEHQYSHVF TVTVRKATNV TKGAIGDMLD TPDPYVELFI
60 70 80 90 100
PSAPDCRKRT KHFNNDVNPV WNETFEFILD PNQDNVLEVT LMDANYVMDE
110 120 130 140 150
TLGMATFPIS SLKLGEKKEV QLTFNNVTEM TLELSLEVCS STDLRFSMAL
160 170 180 190 200
CDEEKKFRQQ RKDNIMQSMK SFLGEENSKN LTTSRDVPVI AVLGSGGGFR
210 220 230 240 250
AMVGFAGVMK ALYESGVLDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPKE
260 270 280 290 300
INQELMNSVS HNPLLLLTPQ KVKRYIEALW NKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIHNRMDT TLSDMKEKVS EAQCALPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMSPDLFGS KFFMGTVVKK YSENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSNS QNKGPTMEEE LENIRLKHLV SNDSSDSEDE SQHPKGTENS
460 470 480 490 500
EANEEYQNSS QESWVQRMLM ALVGDSALFN TREGRAGKVH NFMLGLNLNS
510 520 530 540 550
CYPLSPLADL LTQESVEEDE LDAAVADPDE FERIYEPLDV KSKKIHIVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEI LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPN VFDREGLKEC YVFKPKDTSS EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGLPRESK EEKDFADFDI FDDPNTPFST FNFQYPNEAF KRLHDLMEFN
710 720 730 740
TLNNLDVIKQ AMMESIEYRK ENPSRCSVSL SSVEARRFFN KNNLNNHT
Length:748
Mass (Da):84,979
Last modified:February 1, 1996 - v1
Checksum:i996A5256CA032F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA Translation: AAA53228.1
PIRiI50699
RefSeqiNP_990754.1, NM_205423.1
XP_015146058.1, XM_015290572.1
UniGeneiGga.36724
Gga.3723

Genome annotation databases

EnsembliENSGALT00000079041; ENSGALP00000048216; ENSGALG00000005065
GeneIDi396394
KEGGigga:396394

Similar proteinsi

Entry informationi

Entry nameiPA24A_CHICK
AccessioniPrimary (citable) accession number: P49147
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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