UniProtKB - P49130 (LAMP2_CRIGR)
Lysosome-associated membrane glycoprotein 2
LAMP2
Functioni
Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity).
Plays a role in lysosomal protein degradation in response to starvation (By similarity).
Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).
By similarityGO - Biological processi
- autophagosome maturation Source: UniProtKB
- cellular response to starvation Source: UniProtKB
- chaperone-mediated autophagy Source: UniProtKB
- lysosomal protein catabolic process Source: UniProtKB
- protein stabilization Source: CAFA
- protein targeting Source: UniProtKB
- protein targeting to lysosome involved in chaperone-mediated autophagy Source: UniProtKB
Keywordsi
Biological process | Autophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Lysosome-associated membrane glycoprotein 2Short name: LAMP-2 Short name: Lysosome-associated membrane protein 2 Alternative name(s): CD107 antigen-like family member B Lysosomal membrane glycoprotein B Short name: LGP-B CD_antigen: CD107b |
Gene namesi | Name:LAMP2 Synonyms:LGPB |
Organismi | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) |
Taxonomic identifieri | 10029 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Subcellular locationi
Lysosome
- Lysosome membrane PROSITE-ProRule annotation1 Publication; Single-pass type I membrane protein PROSITE-ProRule annotation
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein PROSITE-ProRule annotation
Endosome
- Endosome membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation
Other locations
- autophagosome membrane By similarity
Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.By similarity
Endosome
- endosome membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- autophagosome membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 29 – 375 | LumenalSequence analysisAdd BLAST | 347 | |
Transmembranei | 376 – 399 | HelicalPROSITE-ProRule annotationAdd BLAST | 24 | |
Topological domaini | 400 – 410 | CytoplasmicPROSITE-ProRule annotationAdd BLAST | 11 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | Sequence analysisAdd BLAST | 28 | |
ChainiPRO_0000017113 | 29 – 410 | Lysosome-associated membrane glycoprotein 2Add BLAST | 382 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 40 ↔ 79 | PROSITE-ProRule annotation | ||
Glycosylationi | 48 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 58 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 71 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 75 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 99 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 123 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 153 ↔ 189 | PROSITE-ProRule annotation | ||
Glycosylationi | 179 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 222 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 232 ↔ 265 | PROSITE-ProRule annotation | ||
Glycosylationi | 242 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 260 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 275 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 300 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 307 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 317 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 331 ↔ 368 | PROSITE-ProRule annotation | ||
Glycosylationi | 356 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Monomer. Forms large homooligomers.
Interacts (via its cytoplasmic region) with HSPA8.
Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity).
Interacts with MLLT11 (By similarity).
Interacts with ABCB9 (By similarity).
Interacts with FURIN (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10029.NP_001233678.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 29 – 192 | First lumenal domainAdd BLAST | 164 | |
Regioni | 193 – 228 | HingeAdd BLAST | 36 | |
Regioni | 229 – 375 | Second lumenal domainAdd BLAST | 147 | |
Regioni | 401 – 404 | Important for binding and subsequent lysosomal degradation of target proteinsBy similarity | 4 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4818, Eukaryota |
OMAi | MATGYES |
OrthoDBi | 1042920at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR018134, LAMP_CS IPR002000, Lysosome-assoc_membr_glycop |
PANTHERi | PTHR11506, PTHR11506, 1 hit |
Pfami | View protein in Pfam PF01299, Lamp, 1 hit |
PRINTSi | PR00336, LYSASSOCTDMP |
PROSITEi | View protein in PROSITE PS00310, LAMP_1, 1 hit PS00311, LAMP_2, 1 hit PS51407, LAMP_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MMCFRLSPVS GSGLVLSCLL LGAVQSYAFE LNLPDSKATC LFAKWKMNFT
60 70 80 90 100
ISYETTTNKT LKTVTISEPH NVTYNGSSCG DDQGVAKIAV QFGSTVSWNV
110 120 130 140 150
TFTKEESHYV IGSIWLVYNT SDNTTFPGAI PKGSATVISS QSIEIPLDDI
160 170 180 190 200
FRCNSLLTFK TGNVVQNYWD IHLQAFVQNG TVSKEEFVCE EDKSVTTVRP
210 220 230 240 250
IIHTTVPPPT TTPTPLPPKV GNYSVSNGNA TCLLATMGLQ LNVTEEKVPF
260 270 280 290 300
IFNINPSTTN FTGSCHPQTA QLRLNNSQIK YLDFIFAVKS ESHFYLKEVN
310 320 330 340 350
VSMYMANGSV FSVANNNLSF WDAPLGSSYM CNKEQVVSVS RTFQINTFNL
360 370 380 390 400
KVQPFNVTKG KYATAQDCSA DEDNFLVPIA VGAALAGVLA LVLLAYFIGL
410
KRHHTGYEQF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19357 mRNA Translation: AAC37683.1 |
RefSeqi | NP_001233678.1, NM_001246749.1 |
Genome annotation databases
GeneIDi | 100689316 |
KEGGi | cge:100689316 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19357 mRNA Translation: AAC37683.1 |
RefSeqi | NP_001233678.1, NM_001246749.1 |
3D structure databases
SMRi | P49130 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10029.NP_001233678.1 |
Genome annotation databases
GeneIDi | 100689316 |
KEGGi | cge:100689316 |
Organism-specific databases
CTDi | 3920 |
Phylogenomic databases
eggNOGi | KOG4818, Eukaryota |
OMAi | MATGYES |
OrthoDBi | 1042920at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR018134, LAMP_CS IPR002000, Lysosome-assoc_membr_glycop |
PANTHERi | PTHR11506, PTHR11506, 1 hit |
Pfami | View protein in Pfam PF01299, Lamp, 1 hit |
PRINTSi | PR00336, LYSASSOCTDMP |
PROSITEi | View protein in PROSITE PS00310, LAMP_1, 1 hit PS00311, LAMP_2, 1 hit PS51407, LAMP_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LAMP2_CRIGR | |
Accessioni | P49130Primary (citable) accession number: P49130 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |