UniProtKB - P49090 (ASNS2_YEAST)
Protein
Asparagine synthetase [glutamine-hydrolyzing] 2
Gene
ASN2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 58200 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.3.5.4
: L-asparagine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).Proteins known to be involved in this subpathway in this organism are:
- Asparagine synthetase [glutamine-hydrolyzing] 2 (ASN2), Asparagine synthetase [glutamine-hydrolyzing] 1 (ASN1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | For GATase activityBy similarity | 1 | |
Binding sitei | 97 | GlutamineBy similarity | 1 | |
Binding sitei | 233 | ATP; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 291 | ATP; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Sitei | 367 | Important for beta-aspartyl-AMP intermediate formationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 365 – 366 | ATPBy similarity | 2 |
GO - Molecular functioni
- asparagine synthase (glutamine-hydrolyzing) activity Source: SGD
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- asparagine biosynthetic process Source: SGD
- glutamine metabolic process Source: UniProtKB-KW
- L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Asparagine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-8963693, Aspartate and asparagine metabolism |
UniPathwayi | UPA00134;UER00195 |
Names & Taxonomyi
Protein namesi | Recommended name: Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)Alternative name(s): Glutamine-dependent asparagine synthetase 2 |
Gene namesi | Name:ASN2 Ordered Locus Names:YGR124W ORF Names:G6358 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGR124W |
SGDi | S000003356, ASN2 |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000056918 | 2 – 572 | Asparagine synthetase [glutamine-hydrolyzing] 2Add BLAST | 571 |
Proteomic databases
MaxQBi | P49090 |
PaxDbi | P49090 |
PRIDEi | P49090 |
2D gel databases
UCD-2DPAGEi | P49090 |
PTM databases
iPTMneti | P49090 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33372, 38 interactors |
DIPi | DIP-5189N |
IntActi | P49090, 5 interactors |
MINTi | P49090 |
STRINGi | 4932.YGR124W |
Miscellaneous databases
RNActi | P49090, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 186 | Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 194 – 545 | Asparagine synthetaseAdd BLAST | 352 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 49 – 53 | Glutamine bindingBy similarity | 5 | |
Regioni | 74 – 76 | Glutamine bindingBy similarity | 3 |
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | KOG0571, Eukaryota |
GeneTreei | ENSGT00940000171863 |
HOGENOMi | CLU_014658_2_2_1 |
InParanoidi | P49090 |
OMAi | LFGYKYT |
Family and domain databases
CDDi | cd01991, Asn_Synthase_B_C, 1 hit cd00712, AsnB, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR006426, Asn_synth_AEB IPR001962, Asn_synthase IPR033738, AsnB_N IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00733, Asn_synthase, 1 hit PF13537, GATase_7, 1 hit |
PIRSFi | PIRSF001589, Asn_synthetase_glu-h, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01536, asn_synth_AEB, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P49090-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCGIFAAFKH EDIHNFKPKA LQLSKKIRHR GPDWSGNAVM NSTIFVHERL
60 70 80 90 100
AIVGLDSGAQ PITSADGEYM LGVNGEIYNH IQLREMCSDY KFQTFSDCEP
110 120 130 140 150
IIPLYLEHDI DAPKYLDGMF AFCLYDSKKD RIVAARDPIG VVTLYMGRSS
160 170 180 190 200
QSPETVYFAS ELKCLTDVCD SIISFPPGHV YDSETDKITR YFTPDWLDEK
210 220 230 240 250
RIPSTPVDYH AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIAAIAARE
260 270 280 290 300
TEKANADANE DNNVDEKQLA GIDDQGHLHT SGWSRLHSFA IGLPNAPDLQ
310 320 330 340 350
AARKVAKFIG SIHHEHTFTL QEGLDALDDV IYHLETYDVT TIRASTPMFL
360 370 380 390 400
LSRKIKAQGV KMVLSGEGSD EIFGGYLYFA QAPSAAEFHT ESVQRVKNLH
410 420 430 440 450
LADCLRANKS TMAWGLEARV PFLDKDFLQL CMNIDPNEKM IKPKEGRIEK
460 470 480 490 500
YILRKAFDTT DEPDVKPYLP EEILWRQKEQ FSDGVGYSWI DGLRDTAERA
510 520 530 540 550
ISDAMFANPK ADWGDDIPTT KEAYWYRLKF DAWFPQKTAA DTVMRWIPKA
560 570
DWGCAEDPSG RYAKIHEKHV SA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 560 | G → S in AAT92877 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83099 Genomic DNA Translation: CAA58159.1 Z72909 Genomic DNA Translation: CAA97135.1 AY692858 Genomic DNA Translation: AAT92877.1 BK006941 Genomic DNA Translation: DAA08217.1 |
PIRi | S55982 |
RefSeqi | NP_011640.1, NM_001181253.1 |
Genome annotation databases
EnsemblFungii | YGR124W_mRNA; YGR124W; YGR124W |
GeneIDi | 853025 |
KEGGi | sce:YGR124W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83099 Genomic DNA Translation: CAA58159.1 Z72909 Genomic DNA Translation: CAA97135.1 AY692858 Genomic DNA Translation: AAT92877.1 BK006941 Genomic DNA Translation: DAA08217.1 |
PIRi | S55982 |
RefSeqi | NP_011640.1, NM_001181253.1 |
3D structure databases
SMRi | P49090 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33372, 38 interactors |
DIPi | DIP-5189N |
IntActi | P49090, 5 interactors |
MINTi | P49090 |
STRINGi | 4932.YGR124W |
PTM databases
iPTMneti | P49090 |
2D gel databases
UCD-2DPAGEi | P49090 |
Proteomic databases
MaxQBi | P49090 |
PaxDbi | P49090 |
PRIDEi | P49090 |
Genome annotation databases
EnsemblFungii | YGR124W_mRNA; YGR124W; YGR124W |
GeneIDi | 853025 |
KEGGi | sce:YGR124W |
Organism-specific databases
EuPathDBi | FungiDB:YGR124W |
SGDi | S000003356, ASN2 |
Phylogenomic databases
eggNOGi | KOG0571, Eukaryota |
GeneTreei | ENSGT00940000171863 |
HOGENOMi | CLU_014658_2_2_1 |
InParanoidi | P49090 |
OMAi | LFGYKYT |
Enzyme and pathway databases
UniPathwayi | UPA00134;UER00195 |
Reactomei | R-SCE-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
PROi | PR:P49090 |
RNActi | P49090, protein |
Family and domain databases
CDDi | cd01991, Asn_Synthase_B_C, 1 hit cd00712, AsnB, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR006426, Asn_synth_AEB IPR001962, Asn_synthase IPR033738, AsnB_N IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00733, Asn_synthase, 1 hit PF13537, GATase_7, 1 hit |
PIRSFi | PIRSF001589, Asn_synthetase_glu-h, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01536, asn_synth_AEB, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASNS2_YEAST | |
Accessioni | P49090Primary (citable) accession number: P49090 Secondary accession number(s): D6VUQ6, E9P8Y0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names