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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

Asns

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] (Asns)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei97GlutamineBy similarity1
Binding sitei256ATP; via carbonyl oxygenBy similarity1
Binding sitei288ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei365Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi363 – 364ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13063
ReactomeiR-RNO-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayi
UPA00134;UER00195

Protein family/group databases

MEROPSiC44.974

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:Asns
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2162 Asns

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2303

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569142 – 561Asparagine synthetase [glutamine-hydrolyzing]Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385N6-acetyllysineBy similarity1
Modified residuei545PhosphothreonineBy similarity1
Modified residuei557PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP49088
PRIDEiP49088

PTM databases

iPTMnetiP49088
PhosphoSitePlusiP49088

Expressioni

Gene expression databases

BgeeiENSRNOG00000007546 Expressed in 10 organ(s), highest expression level in testis
GenevisibleiP49088 RN

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010079

Structurei

3D structure databases

ProteinModelPortaliP49088
SMRiP49088
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 191Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST190
Domaini213 – 536Asparagine synthetaseAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
GeneTreeiENSGT00390000001994
HOGENOMiHOG000027493
HOVERGENiHBG003103
InParanoidiP49088
KOiK01953
OMAiYFHAAPD
PhylomeDBiP49088
TreeFamiTF300603

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 2 hits
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49088-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL
60 70 80 90 100
AVVDPLFGMQ PIRVRKYPYL WLCYNGEIYN HKALQQRFEF EYQTNVDGEI
110 120 130 140 150
ILHLYDKGGI EKTICMLDGV FAFILLDTAN KKVFLGRDTY GVRPLFKALT
160 170 180 190 200
EDGFLAVCSE AKGLVSLKHS TTPFLKVEPF LPGHYEVLDL KPNGKVASVE
210 220 230 240 250
MVKYHHCTDE PLHAIYDSVE KLFPGFEIET VKNNLRILFN NAIKKRLMTD
260 270 280 290 300
RRIGCLLSGG LDSSLVAASL LKQLKEAQVP YALQTFAIGM EDSPDLLAAR
310 320 330 340 350
KVANYIGSEH HEVLFNSEEG IQSLDEVIFS LETYDITTVR ASVGMYLISK
360 370 380 390 400
YIRKNTDSVV IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLKELYLF
410 420 430 440 450
DVLRADRTTA AHGLELRVPF LDHRFSSYYL SLPPEMRIPK DGIEKHLLRE
460 470 480 490 500
TFEDSNLLPK EILWRPKEAF SDGITSVKNS WFKILQDFVE HQVDDAMMSE
510 520 530 540 550
ASQKFPFNTP QTKEGYYYRQ IFEHHYPGRA DWLTHYWMPK WINATDPSAR
560
TLTHYKSTAK A
Length:561
Mass (Da):64,247
Last modified:January 23, 2007 - v3
Checksum:i6C8C6438F424326F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti330S → P in AAA77672 (PubMed:7818476).Curated1
Sequence conflicti330S → P in AAA77671 (PubMed:7818476).Curated1
Sequence conflicti491H → Y in AAA77672 (PubMed:7818476).Curated1
Sequence conflicti491H → Y in AAA77671 (PubMed:7818476).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07202 mRNA Translation: AAA77672.1
U07201 mRNA Translation: AAA77671.1
BC081719 mRNA Translation: AAH81719.1
PIRiS53447
RefSeqiNP_037211.2, NM_013079.2
XP_006236151.1, XM_006236089.3
UniGeneiRn.11172

Genome annotation databases

EnsembliENSRNOT00000010079; ENSRNOP00000010079; ENSRNOG00000007546
ENSRNOT00000083481; ENSRNOP00000071140; ENSRNOG00000007546
GeneIDi25612
KEGGirno:25612
UCSCiRGD:2162 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07202 mRNA Translation: AAA77672.1
U07201 mRNA Translation: AAA77671.1
BC081719 mRNA Translation: AAH81719.1
PIRiS53447
RefSeqiNP_037211.2, NM_013079.2
XP_006236151.1, XM_006236089.3
UniGeneiRn.11172

3D structure databases

ProteinModelPortaliP49088
SMRiP49088
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010079

Chemistry databases

ChEMBLiCHEMBL2303

Protein family/group databases

MEROPSiC44.974

PTM databases

iPTMnetiP49088
PhosphoSitePlusiP49088

Proteomic databases

PaxDbiP49088
PRIDEiP49088

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010079; ENSRNOP00000010079; ENSRNOG00000007546
ENSRNOT00000083481; ENSRNOP00000071140; ENSRNOG00000007546
GeneIDi25612
KEGGirno:25612
UCSCiRGD:2162 rat

Organism-specific databases

CTDi440
RGDi2162 Asns

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
GeneTreeiENSGT00390000001994
HOGENOMiHOG000027493
HOVERGENiHBG003103
InParanoidiP49088
KOiK01953
OMAiYFHAAPD
PhylomeDBiP49088
TreeFamiTF300603

Enzyme and pathway databases

UniPathwayi
UPA00134;UER00195

BioCyciMetaCyc:MONOMER-13063
ReactomeiR-RNO-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

PROiPR:P49088

Gene expression databases

BgeeiENSRNOG00000007546 Expressed in 10 organ(s), highest expression level in testis
GenevisibleiP49088 RN

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 2 hits
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_RAT
AccessioniPrimary (citable) accession number: P49088
Secondary accession number(s): Q66HR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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